標題: Titlebook: Epizootic Hemorrhagic Disease Virus; Methods and Protocol Carrie Batten Book 2024 The Editor(s) (if applicable) and The Author(s), under ex [打印本頁] 作者: Sediment 時間: 2025-3-21 18:11
書目名稱Epizootic Hemorrhagic Disease Virus影響因子(影響力)
作者: 媒介 時間: 2025-3-21 21:29
Recognition of Field Signs, Necropsy Procedures, and Evaluation of Macroscopic Lesions of Cervids Iase study for describing the field signs and necropsy lesions associated with EHD. Here we describe the field signs that commonly define EHD outbreaks in North America, a basic approach to a gross necropsy examination of white-tailed deer, description of the gross lesions that may be present, and di作者: Judicious 時間: 2025-3-22 01:42
Epizootic Hemorrhagic Disease Virus Titration,n of visible cytopathic effect (CPE) in the monolayer of susceptible mammalian cell cultures. However, not all susceptible cell lines demonstrate visible CPE upon EHDV infection, including cell lines such as KC cells, which are derived from the EHDV biological insect vector, .. This chapter presents作者: 地牢 時間: 2025-3-22 07:56
Virus Neutralization Test for Detecting and Quantifying Serum-Neutralizing Antibodies to Epizootic neutralizing antibodies, the VNT can discriminate the EHDV serotype responsible for an infection and provides insights into the time of infection. It is considered the gold standard test for identifying and quantifying antibodies against EHDV serotypes present in test serum samples. The assay is per作者: multiply 時間: 2025-3-22 08:51
https://doi.org/10.1007/978-3-658-36550-9ating the risk assessment. The guidance in this chapter is not just applicable to biosafety professionals, laboratory scientists, or facility managers but can be used by any competent worker familiar with the activity being assessed..This chapter uses the guidance from the WHO to apply the principle作者: Constant 時間: 2025-3-22 16:01
https://doi.org/10.1007/978-3-322-84221-3ase study for describing the field signs and necropsy lesions associated with EHD. Here we describe the field signs that commonly define EHD outbreaks in North America, a basic approach to a gross necropsy examination of white-tailed deer, description of the gross lesions that may be present, and di作者: Constant 時間: 2025-3-22 20:17 作者: 新娘 時間: 2025-3-22 22:44 作者: 情節(jié)劇 時間: 2025-3-23 02:25
1064-3745 Virus: Methods and Protocols .aims to be a?useful up-to-date reference work for researchers currently in the field and to those entering the field..978-1-0716-4037-1978-1-0716-4035-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: LAST 時間: 2025-3-23 07:57
Carrie BattenIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 壁畫 時間: 2025-3-23 13:27
Methods in Molecular Biologyhttp://image.papertrans.cn/f/image/320697.jpg作者: AGATE 時間: 2025-3-23 16:14
https://doi.org/10.1007/978-1-0716-4035-7Orbivirus; Non-contagious viral disease; Ruminants; Serotypes; EHDV作者: 縱火 時間: 2025-3-23 18:37
978-1-0716-4037-1The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: refraction 時間: 2025-3-24 00:17 作者: monopoly 時間: 2025-3-24 03:00 作者: aphasia 時間: 2025-3-24 09:00
https://doi.org/10.1007/978-3-322-84221-3ong ruminant hosts and . species vectors. A variety of domestic and wild ruminant species are susceptible to EHDV infection, but infection outcome is highly variable between species, as well as between individuals of the same species. Thus, this disease system inherently operates at the wildlife-liv作者: browbeat 時間: 2025-3-24 10:57 作者: 1分開 時間: 2025-3-24 18:28 作者: 壓倒性勝利 時間: 2025-3-24 19:41 作者: hemorrhage 時間: 2025-3-25 01:26
https://doi.org/10.1007/978-3-642-58875-4ral component of epizootic hemorrhagic disease virus (EHDV) research, including estimating infectivity, calculating multiplicity of infection, and confirming virus propagation in cell culture. However, the ability to quantify infectious EHDV is also critical for disease control, particularly in the 作者: 信徒 時間: 2025-3-25 04:54
https://doi.org/10.1007/978-3-658-31222-0sitive and specific test. As with most neutralization assays, the EHDV VNT does not react with all virus-targeting antibodies, but specifically with those antibodies that bind to VP2, the outermost capsid structural protein of the virus. The interaction between VP2 and neutralizing antibodies can bl作者: Obvious 時間: 2025-3-25 07:29
https://doi.org/10.1007/978-3-642-57621-8ation lines in a semisolid matrix. Here we describe the preparation of agar gel plates, the method to test serum samples by AGID for the presence of EHDV antibodies, and the interpretation of test results. This test has known cross-reactivity to bluetongue antibodies; therefore positive samples by t作者: 生氣的邊緣 時間: 2025-3-25 12:48 作者: HILAR 時間: 2025-3-25 19:34 作者: 我們的面粉 時間: 2025-3-25 20:27
https://doi.org/10.1007/978-3-662-67801-5d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10?years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous co作者: 易怒 時間: 2025-3-26 04:05
https://doi.org/10.1007/978-3-642-05064-0f viral diseases of livestock. A major advantage of RT-LAMP is that it can be used either as a rapid field test or as a high-throughput screening tool in veterinary laboratories, with sensitivity comparable to the real-time RT-PCR assay. Unlike conventional or qPCR, RT-LAMP uses a strand displacemen作者: 其他 時間: 2025-3-26 04:46 作者: FIG 時間: 2025-3-26 09:57 作者: inflame 時間: 2025-3-26 12:37
https://doi.org/10.1007/978-3-658-43004-7 viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary mechanisms as genetic reassortment. Here, we present a detailed method for obtaining full genome sequ作者: 鄙視 時間: 2025-3-26 19:27
https://doi.org/10.1007/978-3-211-77141-9ding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methods for the infection of . with EHDV in the laboratory via oral infection using an artificial membrane syste作者: commensurate 時間: 2025-3-26 21:20
https://doi.org/10.1007/978-3-531-90655-3quality of starting material. Here we describe the preparation and isolation of epizootic hemorrhagic disease virus (EHDV) from blood and tissue samples in tissue culture flasks (TCFs) through the inoculation of susceptible cell lines including Vero, BHK, and KC cells.作者: dominant 時間: 2025-3-27 02:43
https://doi.org/10.1007/978-3-662-67801-5d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10?years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous control gene—beta-actin.作者: Daily-Value 時間: 2025-3-27 07:19 作者: 長處 時間: 2025-3-27 09:44
Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus,d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10?years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous control gene—beta-actin.作者: Multiple 時間: 2025-3-27 13:57
Managing Risk When Working with Orbiviruses with a Special Emphasis on Epizootic Hemorrhagic Diseassessment provides stepwise guidance for completing a risk assessment, from information gathering and identifying hazards to evaluating the risks, developing, and implementing controls and review..To support the development of a mature safety culture within laboratories, it is important that all staf作者: PRISE 時間: 2025-3-27 18:43
Recognition of Field Signs, Necropsy Procedures, and Evaluation of Macroscopic Lesions of Cervids Iong ruminant hosts and . species vectors. A variety of domestic and wild ruminant species are susceptible to EHDV infection, but infection outcome is highly variable between species, as well as between individuals of the same species. Thus, this disease system inherently operates at the wildlife-liv作者: 先鋒派 時間: 2025-3-27 23:24 作者: perpetual 時間: 2025-3-28 02:52
Purification of Epizootic Hemorrhagic Disease Virus (and Other Orbiviruses) Particles from Infectedosts EHDV, like bluetongue virus (BTV), it has mainly been associated with infection of endothelial cells of capillaries as well as leukocyte subsets. Furthermore, EHDV infects and replicates within its biological vector, . biting midges and .derived cells. A wide range of common laboratory cell lin作者: myopia 時間: 2025-3-28 07:19 作者: 工作 時間: 2025-3-28 10:45
Epizootic Hemorrhagic Disease Virus Titration,ral component of epizootic hemorrhagic disease virus (EHDV) research, including estimating infectivity, calculating multiplicity of infection, and confirming virus propagation in cell culture. However, the ability to quantify infectious EHDV is also critical for disease control, particularly in the 作者: Intentional 時間: 2025-3-28 18:22
Virus Neutralization Test for Detecting and Quantifying Serum-Neutralizing Antibodies to Epizootic sitive and specific test. As with most neutralization assays, the EHDV VNT does not react with all virus-targeting antibodies, but specifically with those antibodies that bind to VP2, the outermost capsid structural protein of the virus. The interaction between VP2 and neutralizing antibodies can bl作者: A精確的 時間: 2025-3-28 19:59 作者: Chronological 時間: 2025-3-29 02:22 作者: adroit 時間: 2025-3-29 04:24
Real-Time RT-PCR for the Diagnosis of Epizootic Hemorrhagic Disease Virus,seases such as epizootic hemorrhagic disease (EHD). Here we provide a protocol for the detection of epizootic hemorrhagic disease virus (EHDV) genetic material in blood and tissue samples, using a real-time RT-PCR that targets a conserved region in segment 9 of the EHDV genome. This protocol can be 作者: facilitate 時間: 2025-3-29 07:56
Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus,d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10?years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous co作者: COMA 時間: 2025-3-29 15:06
Rapid Laboratory Diagnosis of Epizootic Hemorrhagic Disease Virus Using a Loop-Mediated Isothermal f viral diseases of livestock. A major advantage of RT-LAMP is that it can be used either as a rapid field test or as a high-throughput screening tool in veterinary laboratories, with sensitivity comparable to the real-time RT-PCR assay. Unlike conventional or qPCR, RT-LAMP uses a strand displacemen作者: 新陳代謝 時間: 2025-3-29 19:03
End-Point RT-PCR Assays for Detection and Typing of Epizootic Hemorrhagic Disease Virus,h this virus. Viral genome testing can be performed on whole blood under anticoagulant, spleen, and bloody organ homogenates from ruminants. It can also be performed on cell culture following viral isolation tests. Various so-called classical or end-point RT-PCRs will be described, which permit the 作者: 光明正大 時間: 2025-3-29 20:53 作者: lavish 時間: 2025-3-30 01:49
Whole Genome Sequencing of Epizootic Hemorrhagic Disease Virus, viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary mechanisms as genetic reassortment. Here, we present a detailed method for obtaining full genome sequ作者: 船員 時間: 2025-3-30 07:14 作者: 全面 時間: 2025-3-30 08:12
Book 2024, with the aim of facilitating and promoting further research into this understudied .Orbivirus.. Chapters guide readers through methods to enable the characterization of EHDV .in vitro. and .in vivo.. Written in the highly successful?.Methods in Molecular Biology.?series format, chapters include in作者: NAVEN 時間: 2025-3-30 13:36
Book 2024y tips on troubleshooting and avoiding known pitfalls...?..Authoritative and cutting-edge,?.Epizootic Hemorrhagic Disease Virus: Methods and Protocols .aims to be a?useful up-to-date reference work for researchers currently in the field and to those entering the field..作者: 卷發(fā) 時間: 2025-3-30 17:47 作者: 高興一回 時間: 2025-3-31 00:02 作者: Infraction 時間: 2025-3-31 00:51
