標(biāo)題: Titlebook: Extracellular Vesicles in Diagnosis and Therapy; Maurizio Federico,Barbara Ridolfi Book 2022 The Editor(s) (if applicable) and The Author( [打印本頁] 作者: 浮華 時間: 2025-3-21 18:45
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書目名稱Extracellular Vesicles in Diagnosis and Therapy讀者反饋學(xué)科排名
作者: FICE 時間: 2025-3-21 23:01 作者: oncologist 時間: 2025-3-22 01:44 作者: preservative 時間: 2025-3-22 06:57
https://doi.org/10.1007/978-3-642-66092-4n. TEM enables visualization of the exosomes which often present a cup-shaped morphology. Western blot is used to detect commonly expressed exosome-associated proteins. Finally, salivary exosomes isolated using these protocols can further be characterized for downstream analysis according to their c作者: 可以任性 時間: 2025-3-22 09:08
Robert L. Baker,Birgitte R. Mednickly, an immunoaffinity-based EV separation method followed by EV characterization, quantification, and normalization, as well as consecutive miRNA isolation and miRNA profiling by small RNA sequencing, are described.作者: 考博 時間: 2025-3-22 14:00 作者: 考博 時間: 2025-3-22 20:19 作者: Epithelium 時間: 2025-3-22 21:27 作者: NEG 時間: 2025-3-23 03:45
Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytope specific EVs within a known diameter range and fluorescence?range. More specifically, this protocol describes how to determine the concentration of CD61+ (Integrin beta-3, platelet marker), CD235a+ (Glycophorin A, erythrocyte marker), and CD45+ (leukocyte common antigen) EVs in human blood plasma作者: 厭倦嗎你 時間: 2025-3-23 06:21 作者: In-Situ 時間: 2025-3-23 13:18
Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Dely, an immunoaffinity-based EV separation method followed by EV characterization, quantification, and normalization, as well as consecutive miRNA isolation and miRNA profiling by small RNA sequencing, are described.作者: 浸軟 時間: 2025-3-23 14:00 作者: Inflamed 時間: 2025-3-23 18:34
Extracellular Vesicles and Their Use as Vehicles of Immunogensting enveloped viral vectors and virus-like particles. Finally, we describe a both safe and original approach conceived for the induction of strong CTL immunity against antigens uploaded in EVs constitutively released by muscle cells.作者: extract 時間: 2025-3-23 23:40
1064-3745 agnosis and Therapy. serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication..978-1-0716-2343-5978-1-0716-2341-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 閑蕩 時間: 2025-3-24 03:28 作者: Handedness 時間: 2025-3-24 09:24
https://doi.org/10.1007/978-3-319-11155-1 the mouse melanoma cell line B16F10 that yields highly enriched EV samples for subsequent applications such as molecular and functional studies. Our protocol also includes an optional labeling step with the lipophilic dye DiD, which allows tracking of EV uptake by recipient cells in vitro and in vivo.作者: 虛弱 時間: 2025-3-24 11:47 作者: 戰(zhàn)勝 時間: 2025-3-24 15:27
Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells the mouse melanoma cell line B16F10 that yields highly enriched EV samples for subsequent applications such as molecular and functional studies. Our protocol also includes an optional labeling step with the lipophilic dye DiD, which allows tracking of EV uptake by recipient cells in vitro and in vivo.作者: 褻瀆 時間: 2025-3-24 19:24 作者: Indurate 時間: 2025-3-25 02:39 作者: Nuance 時間: 2025-3-25 07:08
Victor J. Ferrans,Jean-Fran?ois Bernaudinanalysis and clinical cancer detection. The assay described in this chapter allows for reliable, sensitive, and specific detection of cancer-derived exosomes using a colorimetric aptasensor that is promising for point-of-care testing.作者: TEN 時間: 2025-3-25 09:00 作者: exostosis 時間: 2025-3-25 13:08 作者: SPALL 時間: 2025-3-25 17:26
Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensoranalysis and clinical cancer detection. The assay described in this chapter allows for reliable, sensitive, and specific detection of cancer-derived exosomes using a colorimetric aptasensor that is promising for point-of-care testing.作者: 離開 時間: 2025-3-25 22:46 作者: LITHE 時間: 2025-3-26 02:28
1064-3745 expertsThis detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and is作者: 陶瓷 時間: 2025-3-26 04:52 作者: GROSS 時間: 2025-3-26 12:00
Understanding Your Influencing Environment, lavage fluid (BALF) and serum without going for an RNA isolation and purification step from EVs. It is an efficient method in several terms such as cost-wise, time, low expertise, and accuracy in results. This method may be helpful in diagnostic blood tests used in medical centers or research laboratories.作者: Urologist 時間: 2025-3-26 15:40 作者: 并排上下 時間: 2025-3-26 18:15 作者: 搖曳 時間: 2025-3-26 21:51 作者: 初學(xué)者 時間: 2025-3-27 04:41
Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesiclesethod for protein extraction, and PolyMAC-based enrichment of phosphopeptides. The combination of these methods provides a highly effective strategy for EV-based phosphoproteome analysis and leads to the discovery of novel phospho-markers previously undetectable.作者: 使殘廢 時間: 2025-3-27 05:39
Maurizio Federico,Barbara RidolfiIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 支形吊燈 時間: 2025-3-27 13:31 作者: 內(nèi)部 時間: 2025-3-27 16:21
https://doi.org/10.1007/978-1-0716-2341-1Disease biomarkers; EV detection and isolation; Macromolecule delivery; Biofluids; Vesicle engineering; I作者: 無辜 時間: 2025-3-27 19:14 作者: 戰(zhàn)勝 時間: 2025-3-27 21:55
Extracellular Vesicles in Diagnosis and Therapy978-1-0716-2341-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Abrupt 時間: 2025-3-28 02:42 作者: epicondylitis 時間: 2025-3-28 08:21
Victor J. Ferrans,Jean-Fran?ois Bernaudinxosomes are potential biomarkers for cancer diagnosis. Biosensors are useful analytical tools for quantification of biomarkers and targeted molecules. An aptasensor uses the aptamer as the biorecognition element to bind to the target and is one main type of biosensors that is promising for exosomes 作者: Vo2-Max 時間: 2025-3-28 12:49
https://doi.org/10.1007/978-3-030-20327-6ifferent cell sources that constitute an attractive target for biomarker studies. However, there is no consensus on the best approach to isolate EVs from blood. Non-EV proteins and lipoproteins in plasma/serum tend to contaminate isolated EVs and confound functional experiments. Here we describe a s作者: 前面 時間: 2025-3-28 16:14
https://doi.org/10.1007/978-3-658-37278-1lating small extracellular vesicles (sEV, diameter: 30–150?nm) are known to play an important role in intercellular communication processes, and proteomics profiling has been explored to develop minimally invasive assays for disease monitoring and diagnosis. Due to the genetic and physiological simi作者: 不適當(dāng) 時間: 2025-3-28 22:17 作者: entrance 時間: 2025-3-29 02:14 作者: reperfusion 時間: 2025-3-29 04:25
Canbing Li,Yijia Cao,Yonghong Kuang,Bin Zhoucted hearts in mouse and nonhuman primate myocardial infarction models. To fully achieve their values, it is essential to establish an efficient method for the isolation of EVs from hPSC-CVPCs. Here we describe the protocols for efficient isolation and characterization of EVs from the conditioned me作者: Isometric 時間: 2025-3-29 09:34
https://doi.org/10.1007/978-3-642-66092-4ciated exosomes with various roles in tumorigenesis and explored their potential as a source of biomarkers for diagnosis and prognosis in cancer research. Exosomes can be isolated from several body fluids, including those that are noninvasively accessible, such as human saliva. This book chapter pro作者: 易改變 時間: 2025-3-29 14:07 作者: amenity 時間: 2025-3-29 16:19
Understanding Your Influencing Environment,urrently, the column-based purification method is used to purify miRNAs from EVs. However, this method of purification is complex, time-consuming, and expensive. Therefore, a simple and cost-effective single-step quantitative reverse transcription-polymerase chain reaction (RT-qPCR) method is requir作者: 護(hù)航艦 時間: 2025-3-29 19:47
https://doi.org/10.1007/978-3-030-93310-4gued plasma-based biomarker discovery. Here, I detail a procedure, which combines EVtrap-based high-recovery EV isolation, phase-transfer surfactant method for protein extraction, and PolyMAC-based enrichment of phosphopeptides. The combination of these methods provides a highly effective strategy f作者: Chandelier 時間: 2025-3-30 01:59
https://doi.org/10.1007/978-1-4684-5239-6eover, lipidomic analyses are useful for identifying novel lipid biomarkers, especially for various metabolic and malignant diseases in humans. Extracellular vesicles (EVs) are lipid bilayer-encapsulated nanoparticles secreted from various cells into the extracellular space. In particular, circulati作者: synovial-joint 時間: 2025-3-30 08:02
Models of family and small community spread,racellular vesicles (EVs). EVs can be distinguished in exosomes and microvesicles. Biological and biomedical research on EVs is an emerging field that is rapidly growing. Many EV features including biogenesis, cell uptake, and functions still require unambiguous elucidation. Nevertheless, it has bee作者: ABOUT 時間: 2025-3-30 09:52
https://doi.org/10.1007/978-3-319-11155-1, and are critically involved in the pathophysiology of various diseases, including tumors. In order to study the interaction of tumor cell-derived EVs with their target cells and to investigate their biological functions in comparison to other tumor cell-released factors, efficient isolation of EVs作者: Myocyte 時間: 2025-3-30 15:36 作者: CRACK 時間: 2025-3-30 17:08 作者: 笨拙處理 時間: 2025-3-30 21:43 作者: 同步左右 時間: 2025-3-31 02:57 作者: 反抗者 時間: 2025-3-31 06:06 作者: 雜役 時間: 2025-3-31 12:18
Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatographyifferent cell sources that constitute an attractive target for biomarker studies. However, there is no consensus on the best approach to isolate EVs from blood. Non-EV proteins and lipoproteins in plasma/serum tend to contaminate isolated EVs and confound functional experiments. Here we describe a s
