標(biāo)題: Titlebook: Extracellular Matrix Protocols; Charles H. Streuli,Michael E. Grant Book 20001st edition Springer Science+Business Media New York 2000 [打印本頁] 作者: 公款 時(shí)間: 2025-3-21 19:30
書目名稱Extracellular Matrix Protocols影響因子(影響力)
作者: 使隔離 時(shí)間: 2025-3-21 21:40
Quantitative Determination of Collagen Crosslinkse stabilized to provide mechanical strength by a series of intermolecular crosslinks. These links are formed by oxidative deamination of the ε-amino group of the single lysine in the amino and carboxy-telopeptides by lysyl oxidase. The aldehyde thus formed reacts with an ε-amino group of a lysine at作者: 荒唐 時(shí)間: 2025-3-22 03:44
Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cellsctivities in basement membrane glycoproteins. This has been particularly valuable in the case of laminins where the relationship between structure and function has been difficult to establish. Several characteristics of the laminins lie at the heart of the problem. First, the molecules are very larg作者: Decongestant 時(shí)間: 2025-3-22 07:57
Recombinant Collagen Trimers from Insect Cells and Yeast their isolation for characterization at the protein level has so far been impossible. Some of the fibril-forming collagens are now in medical use, in applications ranging from the use of type I collagen as a biomaterial and a delivery system for several drugs to trials for the potential of type II 作者: 善于騙人 時(shí)間: 2025-3-22 11:17
Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Str advantages. First, eukaryotic expression can be expected under optimum conditions to produce a large proportion of correctly folded molecules. ECM proteins are made from a group of 25 structurally known (.) and about 200 cDNA derived domains many of which regularly reappear in the different protein作者: 違法事實(shí) 時(shí)間: 2025-3-22 13:17
Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structurtructure, and function. However, their primary structures have revealed that their polypeptide components are frequently comprised of a series of sequence repeats or “modules” (.). This mosaic nature of ECM macromolecules permits their properties to be analyzed by a “dissection” strategy (.), where 作者: 違法事實(shí) 時(shí)間: 2025-3-22 18:42
Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayersion, cell migration, proliferation, differentiation, and gene expression (.,.). Integrins in their native environment can diffuse in the plane of the membrane, and this mobility is required for processes like the assembly of integrins to focal contacts or cell migration (.,.).作者: EXPEL 時(shí)間: 2025-3-22 22:35
Confocal-FRAP Analysis of ECM Molecular Interactions). It is the ECM that defines the architecture, the form, and the biomechanical properties of different tissues (.,.). Among the nonfibrillar macromolecules, the highly charged proteoglycans and hyaluronan are major components that occur at high concentration and greatly influence the movement of so作者: JECT 時(shí)間: 2025-3-23 02:31 作者: Guaff豪情痛飲 時(shí)間: 2025-3-23 07:05
Atomic Force Microspopy Measurements of Intermolecular Binding Strengthining their functional contribution to the maintenance of the anatomical integrity of the adult and embryonal multicellular organism, to fertilization, to blood cell adhesiveness in normal and pathological conditions, to tumor cell adhesion, to parasite-host interactions, and to cellular association作者: Left-Atrium 時(shí)間: 2025-3-23 12:50 作者: 溫和女人 時(shí)間: 2025-3-23 17:24 作者: microscopic 時(shí)間: 2025-3-23 19:53 作者: lymphoma 時(shí)間: 2025-3-24 01:52 作者: 容易做 時(shí)間: 2025-3-24 05:50 作者: 情節(jié)劇 時(shí)間: 2025-3-24 08:46 作者: EXTOL 時(shí)間: 2025-3-24 14:03 作者: pessimism 時(shí)間: 2025-3-24 16:22
https://doi.org/10.1007/978-94-009-4135-9ion, cell migration, proliferation, differentiation, and gene expression (.,.). Integrins in their native environment can diffuse in the plane of the membrane, and this mobility is required for processes like the assembly of integrins to focal contacts or cell migration (.,.).作者: Thyroiditis 時(shí)間: 2025-3-24 21:07
Infections of Cardiac Implantable Devicesspecific macromolecules are bound onto the fibril surface influencing both the assembly and the interaction of the fibril with the surrounding matrix. The electron cryomicroscopy procedures described here form part of a program of work to determine the structure and assembly of tissue fibrils containing surface-associated components.作者: 流動(dòng)性 時(shí)間: 2025-3-25 02:26
Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayersion, cell migration, proliferation, differentiation, and gene expression (.,.). Integrins in their native environment can diffuse in the plane of the membrane, and this mobility is required for processes like the assembly of integrins to focal contacts or cell migration (.,.).作者: palpitate 時(shí)間: 2025-3-25 06:49 作者: 學(xué)術(shù)討論會(huì) 時(shí)間: 2025-3-25 08:44 作者: 過份艷麗 時(shí)間: 2025-3-25 15:18
ECM Macromolecules: Rotary Shadowing and Scanning Transmission Electron MicroscopyThe examination of ECM macromolecules by conventional transmission electron microscopy (TEM) or scanning transmission EM (STEM) can provide important information on macromolecular organization and interactions.作者: monologue 時(shí)間: 2025-3-25 18:25 作者: ECG769 時(shí)間: 2025-3-25 21:42 作者: 到婚嫁年齡 時(shí)間: 2025-3-26 00:23
https://doi.org/10.1007/b105341odification of proteins entering the secretory pathway (.). The procedure involves treating cells grown in culture with the detergent digitonin and isolating the cells free from their cytosolic component (.). The expression of proteins in an SP cell system allows protein assembly to be studied in an作者: Flatter 時(shí)間: 2025-3-26 08:13 作者: Dendritic-Cells 時(shí)間: 2025-3-26 08:41
Infections in Neutropenic Cancer Patientsctivities in basement membrane glycoproteins. This has been particularly valuable in the case of laminins where the relationship between structure and function has been difficult to establish. Several characteristics of the laminins lie at the heart of the problem. First, the molecules are very larg作者: 畸形 時(shí)間: 2025-3-26 16:12 作者: Addictive 時(shí)間: 2025-3-26 19:59 作者: invade 時(shí)間: 2025-3-26 22:18
Principles of Treatment: Decision Making,tructure, and function. However, their primary structures have revealed that their polypeptide components are frequently comprised of a series of sequence repeats or “modules” (.). This mosaic nature of ECM macromolecules permits their properties to be analyzed by a “dissection” strategy (.), where 作者: Magnitude 時(shí)間: 2025-3-27 02:42
https://doi.org/10.1007/978-94-009-4135-9ion, cell migration, proliferation, differentiation, and gene expression (.,.). Integrins in their native environment can diffuse in the plane of the membrane, and this mobility is required for processes like the assembly of integrins to focal contacts or cell migration (.,.).作者: 劇本 時(shí)間: 2025-3-27 09:03 作者: 吹牛者 時(shí)間: 2025-3-27 11:01 作者: 多樣 時(shí)間: 2025-3-27 16:22 作者: 神圣不可 時(shí)間: 2025-3-27 19:16
Clinical Topics in Infectious Diseasey mild to severe and lethal forms (.-.). There are over 100 unique well-characterized chondrodysplasia phenotypes and remarkable progress has been made in the last few years identifying the underlying genetic basis of many of these disorders (.). In most cases, a molecular genetics approach was empl作者: GAVEL 時(shí)間: 2025-3-28 01:09 作者: –LOUS 時(shí)間: 2025-3-28 06:10
Hepatitis C Virus-Associated Cancers,t phenotypic overlaps, like the genodermatoses. Homologous gene targe ing in embryonic stem (ES) cells is increasingly favored over the transgen approach because it allows the study of mutations in homozygosity and without altering the remainder of the genome (1). Homologous gene targeting has been 作者: Cabg318 時(shí)間: 2025-3-28 08:04
Conclusions: Are We There Yet?,s, expression of ECM genes is regulated temporally and spatially. Identification of regulatory elements that direct tissue- and stage-specific expression is important for understanding of the role of ECM genes in cellular differentiation and function. A significant number of ECM genes have been clon作者: indubitable 時(shí)間: 2025-3-28 10:53
Frank J. Jenkins,Linda J. Hoffmanal analysis of protein can be studied by suppression of gene expression, thus introducing a plasmid which expresses an antisense RNA in mammalian cells. Several extracellular matrix proteins require an assembly of subunits to form functional heteromultimers with distinct features. Thus, expression o作者: Spina-Bifida 時(shí)間: 2025-3-28 17:20
1064-3745 . However, we believe that they can readily be transported and established by other researchers. Thus, the purpose of Extracellular Matrix Protocols is to present some of these complicated tech978-1-59259-063-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 無關(guān)緊要 時(shí)間: 2025-3-28 21:43
Book 20001st editionf these technologies are highly sophisticated and are currently available only in a ha- ful of laboratories. However, we believe that they can readily be transported and established by other researchers. Thus, the purpose of Extracellular Matrix Protocols is to present some of these complicated tech作者: 寡頭政治 時(shí)間: 2025-3-28 23:17
Luma Dababneh,William Shomali,Issam I. Raadydro-hydroxylysinonorleucine (A-HLNL), and hydroxylysino-keto-norleucine (HLKNL) may vary considerably between tissues, e.g., rat tail tendon and skin contain A-HLNL whereas cartilage and bone contain predominantly HLKNL.作者: 加強(qiáng)防衛(wèi) 時(shí)間: 2025-3-29 07:07
Home Intravenous Antibiotic Therapy, cells have insufficient levels of it. Prolyl 4-hydroxylase, an α.β. tetramer in vertebrates, plays a central role in the synthesis of all collagens, as 4-hydroxyproline-deficient collagen polypeptide chains cannot form triple helices that are stable at 37°C (.,.). All attempts to assemble an active作者: 逃避系列單詞 時(shí)間: 2025-3-29 07:52
Clinical Topics in Infectious Diseasecal tissue, such as cartilage, for the isolation of mRNA. This problem is compounded by the complex genomic structure of many genes that encode cartilage structural ECM molecules. For most cartilage diseases, a combination of these difficulties has necessitated screening for mutations in a large num作者: conservative 時(shí)間: 2025-3-29 13:58
Frank J. Jenkins,Linda J. Hoffmanerest into tissue-culture cells with success rates approaching 100%. The retrovirus inserts the viral genome into the chromosome of the infected cell permanently, usually without any measurable effect on the viability of the infected cells. The result is an efficient gene-transfer system in which mo作者: 牽索 時(shí)間: 2025-3-29 17:25 作者: 討厭 時(shí)間: 2025-3-29 21:08 作者: Immunotherapy 時(shí)間: 2025-3-30 01:41 作者: 可卡 時(shí)間: 2025-3-30 07:17
Retroviral Delivery of ECM Genes to Cellserest into tissue-culture cells with success rates approaching 100%. The retrovirus inserts the viral genome into the chromosome of the infected cell permanently, usually without any measurable effect on the viability of the infected cells. The result is an efficient gene-transfer system in which mo作者: 沙發(fā) 時(shí)間: 2025-3-30 08:32 作者: 踉蹌 時(shí)間: 2025-3-30 14:48 作者: 蕁麻 時(shí)間: 2025-3-30 18:33 作者: Medicaid 時(shí)間: 2025-3-31 00:45
Enhancer Analysis of the , and , Collagen Genes in Transfected Chondrocytes and Transgenic Miceer, we describe methods for the identification of promoters and enhancers of cartilage collagen genes for the .(.) and .(.) chains using transgenic mice and transient transfection in cell cultures (..).作者: 變異 時(shí)間: 2025-3-31 03:58 作者: homocysteine 時(shí)間: 2025-3-31 07:49 作者: 奴才 時(shí)間: 2025-3-31 09:59
Confocal-FRAP Analysis of ECM Molecular Interactionslutes and water between the tissue and the circulation, and control the access to cells of nutrients, metabolites, growth factors, and chemokines (.–.). This local environmental regulation may have important consequences on cellular functions, especially in tissues with large dense ECMs, such as articular cartilage.作者: Handedness 時(shí)間: 2025-3-31 14:21 作者: 褻瀆 時(shí)間: 2025-3-31 18:39
https://doi.org/10.1007/b105341ents can be added in order to facilitate the study of interaction between proteins within the endoplasmic reticulum (ER) lumen (.,.). Furthermore, as the ER remains morphologically intact, the spatial localization of folding and transport processes within the reticular network may also be examined.作者: 我就不公正 時(shí)間: 2025-3-31 22:10 作者: confide 時(shí)間: 2025-4-1 04:00
Infections in Elderly Patients,he protein folds into such a conformation. Second, most ECM proteins are at least to some extent glycosylated and often heavily so, and the use of the mammalian system offers the best approximation to the sugar structures present in the native form of the molecule.作者: 圣人 時(shí)間: 2025-4-1 07:43 作者: hemophilia 時(shí)間: 2025-4-1 12:24
