標(biāo)題: Titlebook: Expression, Purification, and Structural Biology of Membrane Proteins; Camilo Perez,Timm Maier Book 2020 Springer Science+Business Media, [打印本頁] 作者: DUMMY 時間: 2025-3-21 16:03
書目名稱Expression, Purification, and Structural Biology of Membrane Proteins影響因子(影響力)
書目名稱Expression, Purification, and Structural Biology of Membrane Proteins影響因子(影響力)學(xué)科排名
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書目名稱Expression, Purification, and Structural Biology of Membrane Proteins被引頻次
書目名稱Expression, Purification, and Structural Biology of Membrane Proteins被引頻次學(xué)科排名
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書目名稱Expression, Purification, and Structural Biology of Membrane Proteins年度引用學(xué)科排名
書目名稱Expression, Purification, and Structural Biology of Membrane Proteins讀者反饋
書目名稱Expression, Purification, and Structural Biology of Membrane Proteins讀者反饋學(xué)科排名
作者: 榮幸 時間: 2025-3-21 22:28 作者: Accrue 時間: 2025-3-22 03:02 作者: LINE 時間: 2025-3-22 07:55
Expression and Purification of Membrane Proteins in ,tochondrial membrane proteins developed in our laboratory during the last 15?years. To optimize their expression in a functional form, different promoter systems as well as codon-optimization and complementation strategies were established. Purification approaches were developed which remove the mem作者: 碌碌之人 時間: 2025-3-22 10:58
Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System,st. Recombinant protein production is the first step in the protein tool generation for biochemical and biophysical studies. Here, we provide simplified protocols that facilitate the generation of high-quality virus and initial expression analysis for integral membrane protein targets utilizing the 作者: DAFT 時間: 2025-3-22 13:55 作者: DAFT 時間: 2025-3-22 21:04
Membrane Protein Solubilization and Quality Control: An Example of a Primary Active Transporter,o identify the best-suited detergent, which on the one hand would solubilize large amounts of the target protein but on the other hand would sustain the protein’s activity. Here we describe the solubilization screen and subsequent activity assay we have optimized for the bacterial P-type ATPase KdpF作者: 步兵 時間: 2025-3-23 01:17
GPCR Solubilization and Quality Control,ions, making them interesting pharmacological targets. In order to study their structure and function, GPCRs are traditionally extracted from membranes using detergents. However, due to their hydrophobic nature, intrinsic instability in aqueous solutions, and their?denaturing effects, the?isolation 作者: 刻苦讀書 時間: 2025-3-23 05:01
Affinity Purification of Membrane Proteins,s. One of the most powerful methods for isolation of the membrane protein of interest is affinity purification. This methodology typically relies on engineering an affinity tag into the protein of interest and an affinity resin that specifically recognizes the tag, allowing one to purify the target 作者: 珍奇 時間: 2025-3-23 08:27
Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage,t membrane protein is cloned into an expression plasmid and then introduced into . cells for overexpression. Membranes from bacterial cells are isolated and the tagged target membrane protein is solubilized in detergent and subsequently bound to an affinity matrix. Tagged proteins are commonly elute作者: archaeology 時間: 2025-3-23 11:19
Biotinylation of Membrane Proteins for Binder Selections,tegy determines how the target protein is presented to the binders and thereby directly affects the experimental outcome. This poses specific challenges for membrane proteins due to their inherent lack of stability and limited exposed hydrophilic surfaces. Here we detail methodologies for the select作者: 發(fā)生 時間: 2025-3-23 16:56 作者: 事與愿違 時間: 2025-3-23 21:08
Identifying Conformation-Selective Heavy-Chain-Only Antibodies Against Membrane Proteins by a Therm as well as therapeutics. Their generation has improved with the help of innovative new methods such as the sybody technology; however, identifying conformation-selective compounds against membrane proteins remains a major challenge. In this chapter, we apply a thermal shift scintillation proximity 作者: Infiltrate 時間: 2025-3-23 22:47
Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analysesimportance for chemical and electrical signaling between cells, however, makes them fascinating targets for investigators interested in the regulation of cellular and physiological processes. Since membrane proteins shunt the barrier imposed by the cell membrane, they also serve as entry points for 作者: enflame 時間: 2025-3-24 02:45 作者: COWER 時間: 2025-3-24 09:45 作者: 首創(chuàng)精神 時間: 2025-3-24 12:01 作者: deriver 時間: 2025-3-24 16:09 作者: 陰謀 時間: 2025-3-24 19:57 作者: 意外 時間: 2025-3-25 00:16 作者: FAST 時間: 2025-3-25 06:14 作者: periodontitis 時間: 2025-3-25 08:13
Sarah C. Vigmostad,H.S. Udaykumar methods can easily be used to assess the thermostability and functionality of a GPCR sample exposed to different conditions, such as the?use of various detergents, addition of lipids and ligands, making them valuable for obtaining an optimal sample quality?for structural and functional studies.作者: BRAWL 時間: 2025-3-25 12:50
https://doi.org/10.1007/978-1-4419-6679-7e for enlarging nanobodies on their C-terminal end to generate “macrobodies,” without interfering with their original characteristics. These enlarged nanobodies extend the application as a chaperone in crystallography and can serve to increase the mass for small targets in single particle electron c作者: Prosaic 時間: 2025-3-25 19:38 作者: 沒有貧窮 時間: 2025-3-25 21:44
Bone Density and Mechanical Property,nctional data on these proteins under defined conditions. To that end, different techniques have been developed to stabilize integral membrane proteins in native-like environments that allow both structural and biophysical investigations—amphipols, lipid bicelles, and lipid nanodiscs. In this chapte作者: 苦澀 時間: 2025-3-26 02:01
Geometric Analysis of Light Field Renderingamily of scramblases also function as nonselective ion channels. To better understand the physiological relevance of this channel function as well as its relationship to the scrambling activity of the TMEM16s we also describe in detail an in vitro flux assay to measure nonselective channel activity.作者: 機制 時間: 2025-3-26 05:17 作者: 無動于衷 時間: 2025-3-26 08:36 作者: 高腳酒杯 時間: 2025-3-26 15:16 作者: Meander 時間: 2025-3-26 17:22 作者: 使入迷 時間: 2025-3-26 22:15 作者: triptans 時間: 2025-3-27 03:13 作者: 直覺沒有 時間: 2025-3-27 06:04
Reconstitution of Proteoliposomes for Phospholipid Scrambling and Nonselective Channel Assays,amily of scramblases also function as nonselective ion channels. To better understand the physiological relevance of this channel function as well as its relationship to the scrambling activity of the TMEM16s we also describe in detail an in vitro flux assay to measure nonselective channel activity.作者: Hippocampus 時間: 2025-3-27 09:47
Expression, Purification, and Structural Biology of Membrane Proteins作者: 警告 時間: 2025-3-27 16:55 作者: 吝嗇性 時間: 2025-3-27 21:39
Expression, Purification, and Structural Biology of Membrane Proteins978-1-0716-0373-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: absorbed 時間: 2025-3-27 23:23 作者: 不能仁慈 時間: 2025-3-28 02:15
Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs,t allows the use of membrane-mimicking tools, which can be crucial for a comprehensive understanding of the structure-function relationship of the protein in its native environment. In this chapter we focus on the application of nanodiscs and use our recent studies on the TMEM16 family as an example.作者: 葡萄糖 時間: 2025-3-28 07:30 作者: yohimbine 時間: 2025-3-28 14:18
Hardware for Motion Estimation,onment. The development of the pBEVY vector system has further increased the potential of . as an expression system by creating a method for expressing multiple proteins simultaneously. This vector system is amenable to the expression and purification of multi-subunit protein complexes. Here we desc作者: daredevil 時間: 2025-3-28 18:30
https://doi.org/10.1007/b101495f recombinant membrane proteins. Purification of homogeneous samples of membrane protein expressed in . is a significant bottleneck for researchers, and the protocol we present here for the overexpression and purification of membrane proteins in . will provide a solid basis to develop lab- and prote作者: Muffle 時間: 2025-3-28 19:51
Image and Video-Based Artistic Stylisationrapeutic targets. The gram-positive bacterium . is an attractive option for heterologous membrane protein expression and purification thanks to advantageous characteristics such as mild proteolytic activity and small genome size. Vectors designed for gene transcription under the control of inducible作者: Retrieval 時間: 2025-3-29 02:37 作者: 膽小懦夫 時間: 2025-3-29 04:37 作者: 同來核對 時間: 2025-3-29 10:06 作者: CLAY 時間: 2025-3-29 14:49
o identify the best-suited detergent, which on the one hand would solubilize large amounts of the target protein but on the other hand would sustain the protein’s activity. Here we describe the solubilization screen and subsequent activity assay we have optimized for the bacterial P-type ATPase KdpF作者: Urgency 時間: 2025-3-29 16:06 作者: Immunization 時間: 2025-3-29 21:32 作者: 事情 時間: 2025-3-30 01:28 作者: 洞穴 時間: 2025-3-30 05:02 作者: 群居動物 時間: 2025-3-30 08:12 作者: 圍巾 時間: 2025-3-30 15:21
Image-Based Modeling of Plants and Trees as well as therapeutics. Their generation has improved with the help of innovative new methods such as the sybody technology; however, identifying conformation-selective compounds against membrane proteins remains a major challenge. In this chapter, we apply a thermal shift scintillation proximity 作者: Urologist 時間: 2025-3-30 18:37
Bone Density and Mechanical Property,importance for chemical and electrical signaling between cells, however, makes them fascinating targets for investigators interested in the regulation of cellular and physiological processes. Since membrane proteins shunt the barrier imposed by the cell membrane, they also serve as entry points for 作者: 傳染 時間: 2025-3-30 20:50 作者: 過分自信 時間: 2025-3-31 04:30 作者: 傳授知識 時間: 2025-3-31 07:45 作者: 吸氣 時間: 2025-3-31 12:15
Fractional-Order Control for TITO Systemsure elucidation. Quick and reliable methods to go from protein purification and optimization to grid preparation will significantly improve the reach and power of cryo-EM. Such methods would particularly constitute a tremendous advantage in structural biology of membrane proteins, whose published st作者: transplantation 時間: 2025-3-31 15:27
Ein Ansatz zum ganzheitlichen Image-Design,osphate repeating units anchored to the plasma membrane. The anchor molecule is a lipid-linked disaccharide (anchor-LLD) synthesized at the cytoplasmic leaflet of the membrane. The anchor lipid becomes accessible at the outer leaflet of the membrane after the flippase LtaA catalyzes translocation. R作者: AVOID 時間: 2025-3-31 20:50
Camilo Perez,Timm MaierIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: dry-eye 時間: 2025-3-31 23:51
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/319772.jpg作者: faction 時間: 2025-4-1 02:43 作者: Nuance 時間: 2025-4-1 06:45
Image and Video-Based Artistic Stylisationsion of different gene products. The expressed protein engineered with a suitable tag can be readily detected and purified from crude membrane extracts. The purpose of this protocol chapter is to detail the procedures of cloning, expression, isolation of the membrane vesicles, and affinity purification of a membrane protein of interest in 作者: neutral-posture 時間: 2025-4-1 12:07
Computer-Aided Detection of Lung Cancerof the final protein sample. Here, we describe several protocols for affinity purification of TSPO, a small membrane protein. The techniques we use include immobilized metal affinity chromatography (IMAC) and strep-II tag-based streptavidin affinity chromatography.作者: Narcissist 時間: 2025-4-1 15:28
