作者: TIA742 時(shí)間: 2025-3-21 23:30
Strategies for Undertaking Expressed Sequence Tag (EST) Projects,rposes. They are especially important for enhancing the utility of a genome sequence or for providing a gene catalog for a genome that has not or will not be sequenced. In planning and executing a cDNA project, several criteria must be considered. One should clearly define the project purpose, inclu作者: ascend 時(shí)間: 2025-3-22 02:55 作者: 蒸發(fā) 時(shí)間: 2025-3-22 05:32 作者: CLAY 時(shí)間: 2025-3-22 11:03
Preparation of Full-Length cDNA Libraries: Focus on Metazoans,btraction of redundant transcripts. The use of trehalose and sorbiol stabilizes the activity of the reverse transcriptase leading to efficient cDNA synthesis and the cap-trapping method is used for efficient full-length cDNA selection. Through the incorporation of additional normalization and subtra作者: 出來(lái) 時(shí)間: 2025-3-22 14:46 作者: 出來(lái) 時(shí)間: 2025-3-22 17:25
Generating EST Libraries: ,-Spliced cDNAs,r a fraction) of their mRNAs. This feature may be exploited for the preparation of full-length-enriched cDNA libraries from these organisms (a diverse group including euglenozoa and dinoflagellates, as well as members from five metazoan phyla: Cnidaria, Rotifera, Nematoda, Platyhelminths and Chordat作者: CANDY 時(shí)間: 2025-3-23 00:59
Large-Scale Sequencing and Analytical Processing of ESTs,, their multipurpose uses, such as in probe design for microarrays, determining alternative splicing, verifying open reading frames, and confirming exon/intron and gene boundaries, to name a few, further justify their inclusion in many genomic characterization projects. Hence, there has been a const作者: liaison 時(shí)間: 2025-3-23 02:58
EST Processing: From Trace to Sequence,bjects. Here we describe the usage of a software pipeline (available from .), which has been developed to make the most of EST datasets. This modular software solution is targeted toward small- to medium-sized EST projects and comprises a series of Perl scripts. The software design is based on our e作者: 闡釋 時(shí)間: 2025-3-23 09:27 作者: CON 時(shí)間: 2025-3-23 12:18
EST Databases and Web Tools for EST Projects, emphasis is put on the design of an EST database suited to the specific needs of EST projects and how to choose the most suitable tools. Using TBestDB as an example, we illustrate the essential factors to be considered for database construction and the steps for data population and annotation. This作者: macrophage 時(shí)間: 2025-3-23 14:23
Phylogenomic Analysis of EST Datasets,0 eukaryotic organisms, these exceptional sequence resources offer new opportunities to explore phylogenetic relationships and species diversity. The identification of highly diverse sequences specific to an EST-based sequence dataset offers insights into the extent of genetic novelty within that da作者: 知識(shí) 時(shí)間: 2025-3-23 19:53 作者: IDEAS 時(shí)間: 2025-3-24 02:04 作者: TRUST 時(shí)間: 2025-3-24 04:56 作者: 奇怪 時(shí)間: 2025-3-24 07:40
John ParkinsonEssential reading for any scientist beginning their own EST project.Takes the reader through the entire process of how to successfully complete an EST project.Covers a wide range of cDNA library const作者: 雪白 時(shí)間: 2025-3-24 11:52 作者: uveitis 時(shí)間: 2025-3-24 15:33
https://doi.org/10.1007/978-1-60327-136-3Alternative transcripts; Bur; Expression profiling; Gene discovery; Genetic diversity; Microarray; Phyloge作者: Shuttle 時(shí)間: 2025-3-24 21:18
https://doi.org/10.1007/978-3-658-14848-5cDNA libraries. To date, over 45 million ESTs have been generated from over 1400 different species of eukaryotes. For the most part, EST projects are used to either complement existing genome projects or serve as low-cost alternatives for purposes of gene discovery. However, with improvements in acc作者: 共同生活 時(shí)間: 2025-3-25 03:03
https://doi.org/10.1007/978-3-322-93309-6rposes. They are especially important for enhancing the utility of a genome sequence or for providing a gene catalog for a genome that has not or will not be sequenced. In planning and executing a cDNA project, several criteria must be considered. One should clearly define the project purpose, inclu作者: atopic 時(shí)間: 2025-3-25 05:49 作者: Postmenopause 時(shí)間: 2025-3-25 09:54 作者: 熟練 時(shí)間: 2025-3-25 14:04
Selbsterneuerung eines Immigranten,btraction of redundant transcripts. The use of trehalose and sorbiol stabilizes the activity of the reverse transcriptase leading to efficient cDNA synthesis and the cap-trapping method is used for efficient full-length cDNA selection. Through the incorporation of additional normalization and subtra作者: 胎兒 時(shí)間: 2025-3-25 18:17
Biographieforschung am bewegten Subjekt, become particularly important with recent expanded efforts in complete genome sequencing of understudied, nonmodel eukaryotes such as protists and algae. For these projects, ESTs provide an invaluable source of data for gene identification and prediction of exon-intron boundaries. The generation of作者: 安定 時(shí)間: 2025-3-25 21:41
Politik und Literatur in der DDR,r a fraction) of their mRNAs. This feature may be exploited for the preparation of full-length-enriched cDNA libraries from these organisms (a diverse group including euglenozoa and dinoflagellates, as well as members from five metazoan phyla: Cnidaria, Rotifera, Nematoda, Platyhelminths and Chordat作者: 膝蓋 時(shí)間: 2025-3-26 01:23 作者: insurrection 時(shí)間: 2025-3-26 05:13 作者: 繁重 時(shí)間: 2025-3-26 11:40
https://doi.org/10.1007/978-3-663-10327-1e to sequencing errors and typically define incomplete transcripts, making downstream annotation difficult. Annotation would be greatly improved with robust polypeptide translations. Many current solutions for EST translation require a large number of full-length gene sequences for training purposes作者: Institution 時(shí)間: 2025-3-26 13:17
,Die Frau im ?ffentlichen Leben, emphasis is put on the design of an EST database suited to the specific needs of EST projects and how to choose the most suitable tools. Using TBestDB as an example, we illustrate the essential factors to be considered for database construction and the steps for data population and annotation. This作者: fluffy 時(shí)間: 2025-3-26 18:11
https://doi.org/10.1007/978-3-322-95788-70 eukaryotic organisms, these exceptional sequence resources offer new opportunities to explore phylogenetic relationships and species diversity. The identification of highly diverse sequences specific to an EST-based sequence dataset offers insights into the extent of genetic novelty within that da作者: 重畫(huà)只能放棄 時(shí)間: 2025-3-26 23:14 作者: AV-node 時(shí)間: 2025-3-27 03:16 作者: Arable 時(shí)間: 2025-3-27 05:41 作者: mydriatic 時(shí)間: 2025-3-27 10:00
Expressed Sequence Tags (ESTs)978-1-60327-136-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Arboreal 時(shí)間: 2025-3-27 15:30
Biographieforschung am bewegten Subjekt, serial subtraction of normalized libraries to maintain the discovery rate. Here we describe in detail, protocols for normalization and subtraction of cDNA libraries followed by an example using the toxic dinoflagellate ..作者: Optic-Disk 時(shí)間: 2025-3-27 19:12 作者: 強(qiáng)制性 時(shí)間: 2025-3-27 23:17 作者: Criteria 時(shí)間: 2025-3-28 05:05
Construction of cDNA Libraries: Focus on Protists and Fungi,y are nonaxenic and/or difficult to cultivate. In this chapter, we describe protocols that have been applied successfully to construct and normalize a variety of cDNA libraries from many different species of free-living protists and fungi, and that require only small quantities of cell material.作者: Esophagus 時(shí)間: 2025-3-28 10:10
Generation of Full-Length cDNA Libraries: Focus on Plants,od using the trehalose-thermostabilized reverse transcriptase has been developed and has become an efficient method for construction of high-content full-length cDNA libraries. We have constructed full-length cDNA libraries from various plants and animals using this method. The protocol of the method is described in this chapter.作者: 閑聊 時(shí)間: 2025-3-28 12:31 作者: 男學(xué)院 時(shí)間: 2025-3-28 15:28
1064-3745 ete an EST project.Covers a wide range of cDNA library const.Over the past two decades, expressed sequence tags (ESTs - single pass reads from randomly selected cDNAs), have proven to be a remarkably cost-effective route for the purposes of gene discovery. Gaining in popularity, millions of ESTs hav作者: Delectable 時(shí)間: 2025-3-28 21:35 作者: menopause 時(shí)間: 2025-3-29 01:51
https://doi.org/10.1007/978-3-658-14505-7od using the trehalose-thermostabilized reverse transcriptase has been developed and has become an efficient method for construction of high-content full-length cDNA libraries. We have constructed full-length cDNA libraries from various plants and animals using this method. The protocol of the method is described in this chapter.作者: Irksome 時(shí)間: 2025-3-29 04:56
A Pyramidal Haar-Transform Implementationranscript variants among other utilities. In this review I will discuss the major features of the collection of ESTs available in the public domain giving a special emphasis on how this dataset has been used in studies about human diseases.作者: Mediocre 時(shí)間: 2025-3-29 08:38 作者: 嬉耍 時(shí)間: 2025-3-29 12:17
https://doi.org/10.1007/978-3-531-94139-4sary for the submission of the sequences to the public repository dbEST. . provides facilities for clustering and assembling the ESTs into putative gene objects or unigenes and organizes the data in a relational database. Additional tools are available for annotation and for making the data accessible via the World Wide Web.作者: 放縱 時(shí)間: 2025-3-29 17:44 作者: 有幫助 時(shí)間: 2025-3-29 23:28 作者: largesse 時(shí)間: 2025-3-30 00:55
EST Processing: From Trace to Sequence,sary for the submission of the sequences to the public repository dbEST. . provides facilities for clustering and assembling the ESTs into putative gene objects or unigenes and organizes the data in a relational database. Additional tools are available for annotation and for making the data accessible via the World Wide Web.作者: 溺愛(ài) 時(shí)間: 2025-3-30 07:44
Obtaining Accurate Translations from Expressed Sequence Tags,ave developed a polypeptide prediction pipeline, prot4EST. It incorporates freely available software to produce final translations that are more accurate than those derived from any single method. We describe how this integrated approach goes a long way to overcoming the deficit in training data.作者: cauda-equina 時(shí)間: 2025-3-30 10:45
Selbsterneuerung eines Immigranten,ction steps that eliminate the size bias and expressed gene frequency, it is possible to attain cDNA libraries that include larger or rarely expressed genes. This chapter describes an efficient method to construct a full-length cDNA library, with a focus on metazoan samples.作者: aqueduct 時(shí)間: 2025-3-30 14:43 作者: placebo 時(shí)間: 2025-3-30 17:01 作者: 詞匯表 時(shí)間: 2025-3-30 21:03
Preparation of Full-Length cDNA Libraries: Focus on Metazoans,ction steps that eliminate the size bias and expressed gene frequency, it is possible to attain cDNA libraries that include larger or rarely expressed genes. This chapter describes an efficient method to construct a full-length cDNA library, with a focus on metazoan samples.作者: Intact 時(shí)間: 2025-3-31 04:00
EST Databases and Web Tools for EST Projects, process employs technologies such as ., . and . to build the database and interface, and tools such as . for data processing and annotation. We discuss these in comparison to other available technologies and tools, and explain the reasons for our choices.作者: caldron 時(shí)間: 2025-3-31 08:36
