標題: Titlebook: Expressed Protein Ligation; Methods and Protocol Miquel Vila-Perelló Book 2020 Springer Science+Business Media, LLC, part of Springer Natur [打印本頁] 作者: 味覺沒有 時間: 2025-3-21 19:42
書目名稱Expressed Protein Ligation影響因子(影響力)
書目名稱Expressed Protein Ligation影響因子(影響力)學科排名
書目名稱Expressed Protein Ligation網(wǎng)絡公開度
書目名稱Expressed Protein Ligation網(wǎng)絡公開度學科排名
書目名稱Expressed Protein Ligation被引頻次
書目名稱Expressed Protein Ligation被引頻次學科排名
書目名稱Expressed Protein Ligation年度引用
書目名稱Expressed Protein Ligation年度引用學科排名
書目名稱Expressed Protein Ligation讀者反饋
書目名稱Expressed Protein Ligation讀者反饋學科排名
作者: Diskectomy 時間: 2025-3-21 21:03
Expressed Protein Ligation: General Experimental Protocols,mal ligation sites are discussed. In addition, several methods used to generate the reactive fragments required for EPL are highlighted in practical details. Finally, strategies that one can implement to achieve efficient ligation reactions are presented.作者: 闡明 時間: 2025-3-22 00:23
Book 2020ed proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perfor作者: Toxoid-Vaccines 時間: 2025-3-22 07:43 作者: deadlock 時間: 2025-3-22 10:07
Ulrike Liebert,Hans-Joachim Lauth peptide-based therapeutics by using molecular evolution and/or peptide epitope grafting techniques. In this chapter, we provide protocols to recombinantly produce a natively folded cyclotide making use of a standard bacterial expression system in combination with an intein-mediated backbone cyclization with concomitant oxidative folding.作者: 整頓 時間: 2025-3-22 13:25 作者: 整頓 時間: 2025-3-22 19:10 作者: Rustproof 時間: 2025-3-22 23:56
https://doi.org/10.1007/978-3-642-32675-2ction of inteins can have significant impact for biotechnology applications. Here, we provide biochemical methods to study splicing activity and NMR methods to study intein structure and the catalytic mechanism.作者: Ferritin 時間: 2025-3-23 04:13 作者: MURAL 時間: 2025-3-23 09:28 作者: 有節(jié)制 時間: 2025-3-23 11:04
Patricia M. Davies MCSP Dip. Phys. Ed. for EPL modification of both yeast surface displayed and secreted proteins with bioorthogonal chemical groups are described. These methods allow for the site-specific modification of intein-fused proteins produced in yeast.作者: 外向者 時間: 2025-3-23 16:13 作者: Ceramic 時間: 2025-3-23 20:15
Methods to Study the Structure and Catalytic Activity of ,-Splicing Inteins,ction of inteins can have significant impact for biotechnology applications. Here, we provide biochemical methods to study splicing activity and NMR methods to study intein structure and the catalytic mechanism.作者: 不能妥協(xié) 時間: 2025-3-23 22:45
Peptide Hydrazides as Thioester Equivalents for the Chemical Synthesis of Proteins,peptide hydrazides can be rapidly converted to peptide thioesters, which then selectively react with recombinant protein containing an N-terminal cysteine (Cys) to form a native peptide bond, thereby linking the two peptide segments without isolating any intermediates.作者: Proclaim 時間: 2025-3-24 05:29 作者: 并置 時間: 2025-3-24 06:45
Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation, for EPL modification of both yeast surface displayed and secreted proteins with bioorthogonal chemical groups are described. These methods allow for the site-specific modification of intein-fused proteins produced in yeast.作者: 特征 時間: 2025-3-24 12:02 作者: 溫和女孩 時間: 2025-3-24 18:43 作者: progestin 時間: 2025-3-24 22:52
,Die innere Uhr - Was l?sst uns ticken?,tion of nature’s inteins which are protein modules that catalyze protein splicing. This chapter discusses the basic principles of expressed protein ligation and recent advances and applications in this protein semisynthesis field. Comparative strengths and weaknesses of the method and future challenges are highlighted.作者: 無能性 時間: 2025-3-24 23:32
https://doi.org/10.1007/978-3-531-90468-9mal ligation sites are discussed. In addition, several methods used to generate the reactive fragments required for EPL are highlighted in practical details. Finally, strategies that one can implement to achieve efficient ligation reactions are presented.作者: Insatiable 時間: 2025-3-25 03:27 作者: 夾死提手勢 時間: 2025-3-25 09:34
Identification, Characterization, and Optimization of Split Inteins, the protein splicing reaction, information that can guide further engineering efforts. These techniques provide useful alternatives to common assays that utilize SDS-PAGE to analyze splicing reaction progress.作者: 身心疲憊 時間: 2025-3-25 11:50
,Die innere Uhr - Was l?sst uns ticken?,N-cysteine containing synthetic peptides in a chemoselective ligation. The recombinant protein C-terminal thioesters are produced by exploiting the action of nature’s inteins which are protein modules that catalyze protein splicing. This chapter discusses the basic principles of expressed protein li作者: ENACT 時間: 2025-3-25 15:51 作者: formula 時間: 2025-3-25 23:48 作者: 強行引入 時間: 2025-3-26 01:01 作者: septicemia 時間: 2025-3-26 05:30 作者: Rustproof 時間: 2025-3-26 12:18
Maarten J. Arentsen,Doris Fuchsession. Peptide hydrazides are widely used as thioester equivalents in the total chemical synthesis and semisynthesis of proteins as they can be easily prepared using standard solid phase peptide synthesis (SPPS) and recombinant peptide techniques. Via treatment with NaNO. and subsequent thiolysis, 作者: 清醒 時間: 2025-3-26 16:38 作者: 不斷的變動 時間: 2025-3-26 19:00
https://doi.org/10.1007/978-3-663-20507-4ir ultrafast splicing kinetics allow for the efficient production of native proteins from two separate polypeptides both in vitro and in cells. They can also be used to generate proteins with C-terminal thioesters for downstream applications. In this chapter, we describe a method based on a genetica作者: HERE 時間: 2025-3-26 23:56
https://doi.org/10.1007/978-3-7091-9833-9o driven innovations in protein science. Expressed protein ligation (EPL) is a widely used method to generate chemically modified proteins. However, EPL has some limitations, particularly relevant to modify challenging proteins such as antibodies. The method termed streamlined expressed protein liga作者: 妨礙議事 時間: 2025-3-27 03:47 作者: FEAT 時間: 2025-3-27 05:58
Patricia M. Davies MCSP Dip. Phys. Ed.i of proteins. In this way, protein activity can be preserved while functionalizing the target protein with a wide range of chemical handles. Here, we describe methods for EPL-based modification of proteins produced by yeast, employing an engineered, non–self-cleaving intein known as 202-08. Methods作者: Formidable 時間: 2025-3-27 12:34 作者: Pelvic-Floor 時間: 2025-3-27 15:30
https://doi.org/10.1007/978-3-531-92156-3ons form a combinatorial language which defines the functional state of the underlying genome. As each histone type exists in two copies in a nucleosome, the modification patterns can differ between the individual histones, resulting in asymmetry and increasing combinatorial complexity. To systemati作者: Budget 時間: 2025-3-27 17:50 作者: 記憶 時間: 2025-3-28 01:42 作者: 變色龍 時間: 2025-3-28 02:49 作者: 褲子 時間: 2025-3-28 08:15 作者: scrutiny 時間: 2025-3-28 11:12
https://doi.org/10.1007/978-1-0716-0434-2protein engineering; split inteins; C-terminal thioesters; hydrazide oxidation; protein splicing作者: CRAFT 時間: 2025-3-28 18:25 作者: FOLD 時間: 2025-3-28 21:27
Expressed Protein Ligation978-1-0716-0434-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Mettle 時間: 2025-3-29 01:25 作者: grenade 時間: 2025-3-29 06:52
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/319767.jpg作者: 通知 時間: 2025-3-29 11:13
Protein Splicing: From the Foundations to the Development of Biotechnological Applications,ent of biotechnological applications including the study of disease progression and validation of potential drug candidates. Here, we review almost three decades of research to uncover the chemical and biochemical enigmas of protein splicing and the development of inteins as potent protein engineeri作者: Moderate 時間: 2025-3-29 15:18 作者: 發(fā)炎 時間: 2025-3-29 18:26 作者: Kidnap 時間: 2025-3-29 23:22 作者: confide 時間: 2025-3-30 01:24 作者: diskitis 時間: 2025-3-30 06:17
,Investigating the Effects of O-GlcNAc Modifications in Parkinson’s Disease Using Semisynthetic α-Synuclein bearing an O-GlcNAc modification at threonine 72, termed α-synuclein(gT72). However, this general approach utilizing two recombinant fragments and one synthetic peptide is applicable to other sites and types of modifications and should be transferable to various other protein targets, includ作者: 黃瓜 時間: 2025-3-30 09:22
,Highly Precise Protein Semisynthesis through Ligation–Desulfurization Chemistry in Combination withoesters, withstands standard ligation, desulfurization and reversed phase HPLC conditions, and can be smoothly removed. We have previously demonstrated the utility of this protecting group through the semisynthesis of two model proteins, human small heat shock protein Hsp27 and Prion protein, in whi作者: 出處 時間: 2025-3-30 15:20
Book 2020o obtain precisely modified proteins.Written in the highly successful?.Methods in MolecularBiology?.series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troublesho作者: fidelity 時間: 2025-3-30 19:09 作者: 注射器 時間: 2025-3-30 23:38
https://doi.org/10.1007/978-3-662-67219-8oval conditions. These new synthetic methods enable the easy introduction of PTMs in the thioester fragment. In this chapter, we describe an approach for the synthesis and use of C-terminal α-N-acylbenzimidazolinone (Nbz) and α-N-acyl-N′-methylbenzimidazolinone (MeNbz) peptides in NCL. Following ste作者: 含糊 時間: 2025-3-31 03:52 作者: Certainty 時間: 2025-3-31 07:54 作者: 火光在搖曳 時間: 2025-3-31 12:48
https://doi.org/10.1007/978-3-531-92156-3ynthesis of H3, coupled with the use of the protease cleavable “.-tag,” we provide an efficient and traceless method for the controlled semisynthesis and reconstitution of asymmetrically modified nucleosomes.作者: 供過于求 時間: 2025-3-31 15:21 作者: 后天習得 時間: 2025-3-31 19:43 作者: 抑制 時間: 2025-3-31 22:17 作者: deforestation 時間: 2025-4-1 05:24
Methods and Applications of Expressed Protein Ligation,N-cysteine containing synthetic peptides in a chemoselective ligation. The recombinant protein C-terminal thioesters are produced by exploiting the action of nature’s inteins which are protein modules that catalyze protein splicing. This chapter discusses the basic principles of expressed protein li作者: 枕墊 時間: 2025-4-1 08:39
Protein Splicing: From the Foundations to the Development of Biotechnological Applications,odifications, and biophysical probes into proteins of any size. This methodology has been developed based on the knowledge obtained from protein splicing. Protein splicing is a multistep biochemical reaction that includes the concomitant cleavage and formation of peptide bonds carried out by self-pr作者: Constrain 時間: 2025-4-1 12:24
Identification, Characterization, and Optimization of Split Inteins,es, most notably protein ligation. The development of these approaches is dependent on the identification and/or design of split inteins with robust activity, stability, and solubility. Here, we describe two approaches to characterize and compare the activities of newly identified or engineered spli作者: Anticlimax 時間: 2025-4-1 17:52
Methods to Study the Structure and Catalytic Activity of ,-Splicing Inteins,tein splicing has been studied by overexpression in . of intein fusion proteins with nonnative exteins. Inteins can be used to generate reactive α-thioesters, as well as proteins with N-terminal Cys residues, to facilitate expressed protein ligation. As such, a more detailed understanding of the fun作者: Junction 時間: 2025-4-1 20:54
