標題: Titlebook: Experiments in Molecular Biology; Robert J. Slater Book 1986 Springer Science+Business Media LLC 1986 biology.Colon.DNA.enzyme.enzymes.Esc [打印本頁] 作者: 獨裁者 時間: 2025-3-21 17:07
書目名稱Experiments in Molecular Biology影響因子(影響力)
書目名稱Experiments in Molecular Biology影響因子(影響力)學科排名
書目名稱Experiments in Molecular Biology網(wǎng)絡公開度
書目名稱Experiments in Molecular Biology網(wǎng)絡公開度學科排名
書目名稱Experiments in Molecular Biology被引頻次
書目名稱Experiments in Molecular Biology被引頻次學科排名
書目名稱Experiments in Molecular Biology年度引用
書目名稱Experiments in Molecular Biology年度引用學科排名
書目名稱Experiments in Molecular Biology讀者反饋
書目名稱Experiments in Molecular Biology讀者反饋學科排名
作者: 群居男女 時間: 2025-3-21 23:19 作者: Mettle 時間: 2025-3-22 01:53 作者: octogenarian 時間: 2025-3-22 07:29
Southern Blotting,The Southern blotting technique was developed by E. Southern in 1975 (.) to facilitate the identification of specific sequences within a complex mixture of DNA fragments. The technique itself has been modified over the years and is now an extremely sensitive and fast analytical technique used in almost every molecular biology laboratory.作者: 死亡 時間: 2025-3-22 10:53
Preparative Electrophoresis,Extraction of DNA or RNA fragments from agarose or acrylamide gels is an important tool in genetic engineering. A mixture of fragments can be separated by electrophoresis, and a particular fragment eluted and used for subsequent analysis or cloning.作者: 錯 時間: 2025-3-22 15:25
Vyacheslav Kharchenko,Oleg Illiashenkoodern molecular biology and provide an interesting contrast to the more traditional large-scale biochemical methodologies. The experiment itself will fit into a 3-h practical session and requires no expensive laboratory equipment.作者: 錯 時間: 2025-3-22 20:15
Microisolation and Microanalysis of Plasmid DNA,odern molecular biology and provide an interesting contrast to the more traditional large-scale biochemical methodologies. The experiment itself will fit into a 3-h practical session and requires no expensive laboratory equipment.作者: mortgage 時間: 2025-3-23 01:14
https://doi.org/10.1007/978-1-60327-405-0biology; Colon; DNA; enzyme; enzymes; Escherichia coli; Filtration; molecular biology; nucleic acid; protein; 作者: 微粒 時間: 2025-3-23 04:08
Cleveland Amory and the Fund for Animalss of genetic engineering in theory and would like to supplement their knowledge with practical experience. Many DNA manipulations, however, make use of equipment that is unsuitable for introductory classes with possibly large numbers of students, and require more time than that available in already 作者: 創(chuàng)造性 時間: 2025-3-23 09:33 作者: 鳥籠 時間: 2025-3-23 10:56 作者: perimenopause 時間: 2025-3-23 13:51 作者: Firefly 時間: 2025-3-23 19:25
https://doi.org/10.1007/978-3-319-55595-9 by considering the following problem. A scientific group wishes to clone in bacteria a particular human chromosomal gene. There would be no difficulty obtaining human DNA, cutting it with a restriction endonuclease, and ligating the many fragments obtained into vector molecules. This ligation mix c作者: endure 時間: 2025-3-23 22:37 作者: Obstreperous 時間: 2025-3-24 05:13
Maryna Kolisnyk,Vyacheslav Kharchenkoually associated. Cells of plants and animals contain from 1 to 100 pg of DNA per nucleus. This does not sound like a lot until one realizes that is represents 100–10,000 times as much DNA as in an . chromosome. When faced with such vast complexity, it is obviously an advantage if the DNA can be sep作者: negotiable 時間: 2025-3-24 09:40 作者: 貪婪的人 時間: 2025-3-24 12:09
https://doi.org/10.1057/978-1-137-58736-7A molecules are involved in the expression of the genetic material (DNA) and are, in fact, the initial products of gene expression that go on to direct protein synthesis. Knowledge of the structure, metabolism, and mode of action of RNA in protein synthesis is therefore fundamental to our understand作者: Magisterial 時間: 2025-3-24 15:27
Ionic Liquids in the Nuclear Industry, simple, but nevertheless achieves very reproducible results and high resolution. RNA is a polyanion and will therefore migrate toward the positive electrode in an electric field. If the migration occurs through a gel matrix of carefully chosen pore size, the mobility of the RNA molecules is related作者: Gentry 時間: 2025-3-24 21:57
Jan Nill,Ulrich Petschow,Matthias Jahnckeh prokaryotic and eukaryotic systems have been developed, but for this experiment only a eukaryotic system will be considered. In 1973, Roberts and Paterson (.) demonstrated that a cell-free extract of wheat germ would faithfully translate rabbit globin mRNA and tobacco mosaic virus RNA to yield ful作者: 殺死 時間: 2025-3-25 00:32 作者: 未開化 時間: 2025-3-25 05:38 作者: coalition 時間: 2025-3-25 08:57
https://doi.org/10.1007/978-981-16-6383-3cellulose paper. Once transferred to the immobilizing medium, a variety of analytical procedures may be carried out on the proteins that would otherwise have proved difficult or impossible in the gel. Such procedures may include hybridization with labeled DNA or RNA probes, detection with antibodies作者: 發(fā)電機 時間: 2025-3-25 14:07 作者: Anthrp 時間: 2025-3-25 19:22 作者: nonsensical 時間: 2025-3-25 23:52
978-0-89603-082-4Springer Science+Business Media LLC 1986作者: Phenothiazines 時間: 2025-3-26 02:18
Experiments in Molecular Biology978-1-60327-405-0Series ISSN 1949-2448 Series E-ISSN 1949-2456 作者: ADJ 時間: 2025-3-26 07:35 作者: CUMB 時間: 2025-3-26 10:20
Rock-Filled Concrete with Manufactured Sand,e chromosomal DNA, but can contain genes, not only for antibiotic resistance, but also for antibiotic synthesis, toxin production, nitrogen fixation, production of degradative enzymes, and conjugation. The plasmids can be transferred from one cell to another, and therefore act as carriers or “vectors” of the extrachromosomal genes they contain.作者: CRAMP 時間: 2025-3-26 14:31
Green IT: Technologies and Applicationsive to main-band and other satellite DNAs so that they can be resolved separately. Cesium chloride-actinomycin D gradients have been used to isolate sea urchin histone genes (.) and plant ribosomal DNA (.), and to separate genes coding for plant 25S and 18S from 5S RNA genes (.) and as a prelude to the cloning of ribosomal DNA (.).作者: hedonic 時間: 2025-3-26 19:19
https://doi.org/10.1057/978-1-137-58736-7ing of the control of gene expression, cell division, growth, and development. Furthermore, the recent spectacular advances in genetic engineering depend on a working knowledge of RNA synthesis and its control.作者: 人類學家 時間: 2025-3-26 21:33 作者: blithe 時間: 2025-3-27 03:04 作者: 狼群 時間: 2025-3-27 08:57
Isolation of Plasmid DNA,e chromosomal DNA, but can contain genes, not only for antibiotic resistance, but also for antibiotic synthesis, toxin production, nitrogen fixation, production of degradative enzymes, and conjugation. The plasmids can be transferred from one cell to another, and therefore act as carriers or “vectors” of the extrachromosomal genes they contain.作者: Bouquet 時間: 2025-3-27 10:22
Purification of Plant Ribosomal DNA,ive to main-band and other satellite DNAs so that they can be resolved separately. Cesium chloride-actinomycin D gradients have been used to isolate sea urchin histone genes (.) and plant ribosomal DNA (.), and to separate genes coding for plant 25S and 18S from 5S RNA genes (.) and as a prelude to the cloning of ribosomal DNA (.).作者: Spinous-Process 時間: 2025-3-27 15:03
The Extraction and Affinity Chromatography of Messenger RNA,ing of the control of gene expression, cell division, growth, and development. Furthermore, the recent spectacular advances in genetic engineering depend on a working knowledge of RNA synthesis and its control.作者: immunity 時間: 2025-3-27 20:44
SDS Polyacrylamide Gel Electrophoresis of Proteins, variation made it difficult to directly compare one sample with another. This problem was overcome by the introduction of the slab gel system (.), in which a large number of samples can be compared on a single run. It is this system that is described in this experiment.作者: alleviate 時間: 2025-3-27 21:57 作者: analogous 時間: 2025-3-28 05:28 作者: Sinus-Rhythm 時間: 2025-3-28 08:30 作者: 造反,叛亂 時間: 2025-3-28 13:52
Book 1986 new laboratory techniques that allow very precise fractionation and analysis of nucleic acids and proteins, as well as the construction of recom- binant DNA molecules that can then be cloned and expressed in host cells. Progress has been so rapid that there has been a shortfall in the training of a作者: 共同生活 時間: 2025-3-28 16:20 作者: 江湖騙子 時間: 2025-3-28 19:09 作者: 令人苦惱 時間: 2025-3-29 01:33 作者: 責怪 時間: 2025-3-29 06:50
https://doi.org/10.1007/978-3-319-55595-9ired gene. Human chromosomal genes are most unlikely to be expressed in bacteria, and even less likely to give the colony a recognizable new phenotype. So how is it possible to select the one required clone from perhaps 100,000 others? By directly probing for the required DNA segment, colony hybridization provides a solution to this problem.作者: 不利 時間: 2025-3-29 10:31 作者: 厚顏無恥 時間: 2025-3-29 12:10
Restriction Endonuclease Digestion and Agarose Gel Electrophoresis of DNA,ll molecules of the genomic DNA must be cut identically, so that each gives the same set of restriction fragments. With luck, one of these fragments will contain the desired gene, and further restriction of the isolated fragment may allow recovery of a smaller fragment containing little more than the gene.作者: Annotate 時間: 2025-3-29 16:44 作者: Exuberance 時間: 2025-3-29 23:17
Protein (Western) Blotting with Immunodetection,us to the method used to transfer DNA from agarose gels (the Southern Blot, . Chapter 5) and has been given the name “Western” blotting (.–.). There are a number of advantages to working with a protein blot rather than the original gel. These include:作者: echnic 時間: 2025-3-30 03:12
DNA Isolation and Transformation in ,,s of genetic engineering in theory and would like to supplement their knowledge with practical experience. Many DNA manipulations, however, make use of equipment that is unsuitable for introductory classes with possibly large numbers of students, and require more time than that available in already 作者: Morbid 時間: 2025-3-30 06:18 作者: CHASM 時間: 2025-3-30 11:15 作者: pulse-pressure 時間: 2025-3-30 14:59 作者: objection 時間: 2025-3-30 18:39
Detection of Plasmid by Colony Hybridization, by considering the following problem. A scientific group wishes to clone in bacteria a particular human chromosomal gene. There would be no difficulty obtaining human DNA, cutting it with a restriction endonuclease, and ligating the many fragments obtained into vector molecules. This ligation mix c作者: 特別容易碎 時間: 2025-3-30 22:05 作者: 有幫助 時間: 2025-3-31 04:56
Isopycnic Centrifugation of DNA,ually associated. Cells of plants and animals contain from 1 to 100 pg of DNA per nucleus. This does not sound like a lot until one realizes that is represents 100–10,000 times as much DNA as in an . chromosome. When faced with such vast complexity, it is obviously an advantage if the DNA can be sep作者: 才能 時間: 2025-3-31 08:54
Purification of Plant Ribosomal DNA,sed in the preceeding chapter. This chapter describes a modification of the procedure that enhances the separation of specific DNA sequences from main-band DNA. The method is based on the fact that actinomycin D binds preferentially to DNA molecules with a high-GC content. This . their density relat作者: 輕快走過 時間: 2025-3-31 09:52
The Extraction and Affinity Chromatography of Messenger RNA,A molecules are involved in the expression of the genetic material (DNA) and are, in fact, the initial products of gene expression that go on to direct protein synthesis. Knowledge of the structure, metabolism, and mode of action of RNA in protein synthesis is therefore fundamental to our understand作者: 過份艷麗 時間: 2025-3-31 16:06
Agarose Gel Electrophoresis of RNA, simple, but nevertheless achieves very reproducible results and high resolution. RNA is a polyanion and will therefore migrate toward the positive electrode in an electric field. If the migration occurs through a gel matrix of carefully chosen pore size, the mobility of the RNA molecules is related作者: BIDE 時間: 2025-3-31 19:59
In Vitro Protein Synthesis,h prokaryotic and eukaryotic systems have been developed, but for this experiment only a eukaryotic system will be considered. In 1973, Roberts and Paterson (.) demonstrated that a cell-free extract of wheat germ would faithfully translate rabbit globin mRNA and tobacco mosaic virus RNA to yield ful作者: AMEND 時間: 2025-3-31 22:44
