標(biāo)題: Titlebook: Exosomes and Microvesicles; Methods and Protocol Andrew F Hill Book 2017 Springer Science+Business Media LLC 2017 extracellular vesicles.ce [打印本頁(yè)] 作者: Glitch 時(shí)間: 2025-3-21 17:19
書(shū)目名稱Exosomes and Microvesicles影響因子(影響力)
書(shū)目名稱Exosomes and Microvesicles影響因子(影響力)學(xué)科排名
書(shū)目名稱Exosomes and Microvesicles網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱Exosomes and Microvesicles網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱Exosomes and Microvesicles被引頻次
書(shū)目名稱Exosomes and Microvesicles被引頻次學(xué)科排名
書(shū)目名稱Exosomes and Microvesicles年度引用
書(shū)目名稱Exosomes and Microvesicles年度引用學(xué)科排名
書(shū)目名稱Exosomes and Microvesicles讀者反饋
書(shū)目名稱Exosomes and Microvesicles讀者反饋學(xué)科排名
作者: CESS 時(shí)間: 2025-3-21 20:45
A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extraclarge vesicles, subcellular organelles, or debris. The level of EV secretion and content are determined by assays for acetylcholinesterase activity and total protein estimation, and exosomal identification and protein content are analyzed by Western blot and immuno-electron microscopy. Additionally,作者: instructive 時(shí)間: 2025-3-22 02:55
Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission,aracterized this cell-free form of prions and showed that infectivity was associated with exosomes. Since exosomes are produced by a variety of cells, including cells that actively accumulate prions, they could be a vehicle for infectivity in body fluids and could participate to the dissemination of作者: 放棄 時(shí)間: 2025-3-22 08:11
Bioinformatics Tools for Extracellular Vesicles Research, ExoCarta, EVpedia, and Vesiclepedia catalog multiple datasets. These resources aid in elucidating molecular mechanism and pathophysiology underlying different disease conditions from which EVs are isolated. Here, the existing bioinformatics tools to perform integrated analysis to identify key funct作者: bacteria 時(shí)間: 2025-3-22 10:29 作者: Iatrogenic 時(shí)間: 2025-3-22 14:37 作者: Iatrogenic 時(shí)間: 2025-3-22 20:51
Fundamental Theorems in Fuzzy Normed Spaces,large vesicles, subcellular organelles, or debris. The level of EV secretion and content are determined by assays for acetylcholinesterase activity and total protein estimation, and exosomal identification and protein content are analyzed by Western blot and immuno-electron microscopy. Additionally,作者: 一條卷發(fā) 時(shí)間: 2025-3-22 22:34 作者: 避開(kāi) 時(shí)間: 2025-3-23 03:30 作者: 褻瀆 時(shí)間: 2025-3-23 07:31 作者: badinage 時(shí)間: 2025-3-23 10:04 作者: Goblet-Cells 時(shí)間: 2025-3-23 14:51
1064-3745 ation advice from the experts.Includes supplementary materia.This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle作者: FACT 時(shí)間: 2025-3-23 19:59
Sungsoon Hwang,Jean-Claude Thillodologies to characterize nano-sized EVs have been developed. This chapter aims to summarize physical principles of novel and conventional technologies to be used in the EV field and to discuss advantages and limitations.作者: enumaerate 時(shí)間: 2025-3-24 01:10 作者: diathermy 時(shí)間: 2025-3-24 06:18
Fuzzy Multiple Objective Decision Making,erform multiplexed phenotyping of EVs in an open platform. This protocol outlines the microarray printing procedure followed by the steps of capture and detection of small extracellular vesicles from plasma/serum or cell culture supernatants. The principles of data treatment and analysis are thoroughly described as well.作者: trigger 時(shí)間: 2025-3-24 07:52 作者: 哺乳動(dòng)物 時(shí)間: 2025-3-24 11:54
Studies in Fuzziness and Soft Computing from exosomes, thus making library construction difficult. Here, this protocol describes an optimized protocol for constructing small RNA libraries from low yields of RNA, in particular, extracted from exosomes isolated from biological fluids.作者: opinionated 時(shí)間: 2025-3-24 16:12
,Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM? an from exosomes, thus making library construction difficult. Here, this protocol describes an optimized protocol for constructing small RNA libraries from low yields of RNA, in particular, extracted from exosomes isolated from biological fluids.作者: 輕快帶來(lái)危險(xiǎn) 時(shí)間: 2025-3-24 22:43 作者: 大廳 時(shí)間: 2025-3-25 02:07 作者: liaison 時(shí)間: 2025-3-25 06:17
https://doi.org/10.1007/978-3-030-61282-5ld since their initial discovery. Here, we describe the use of two transmission electron microscopy (TEM) techniques for imaging and quantifying EVs. Cryo-TEM combined with receptor-specific gold labeling is applied to reveal the morphology, size, and phenotype of EVs, while their enumeration is achieved after high-speed sedimentation on EM grids.作者: BRAWL 時(shí)間: 2025-3-25 10:06
https://doi.org/10.1007/978-981-99-5154-3n cells. Therefore, EVs are increasingly being considered as potential therapeutic siRNA delivery systems..In this chapter we describe a method for preparing siRNA-loaded EVs, including a robust, scalable method to isolate them from cell culture supernatants.作者: 憤怒歷史 時(shí)間: 2025-3-25 15:32 作者: 責(zé)問(wèn) 時(shí)間: 2025-3-25 18:54 作者: Obvious 時(shí)間: 2025-3-25 22:37 作者: Pulmonary-Veins 時(shí)間: 2025-3-26 02:31
Preparation and Isolation of siRNA-Loaded Extracellular Vesicles,n cells. Therefore, EVs are increasingly being considered as potential therapeutic siRNA delivery systems..In this chapter we describe a method for preparing siRNA-loaded EVs, including a robust, scalable method to isolate them from cell culture supernatants.作者: Cleave 時(shí)間: 2025-3-26 06:44 作者: 冷漠 時(shí)間: 2025-3-26 10:54
Cluster Analysis for Relational Data,ermine their concentration. This chapter describes a method for identifying the size and concentration of a subpopulation of vesicles in biological samples, using nanoparticle tracking analysis. Characterization of distinct exosomes is enabled by specific marker antibodies, coupled to fluorescent quantum dots.作者: 羊齒 時(shí)間: 2025-3-26 13:09
Structural Properties of Basic Operations,ome release by increasing glutamatergic synapse activity, and purify exosomes by differential centrifugations followed by density separation using sucrose gradients. These protocols allow purification of neuronal exosomes released within minutes of activation of glutamatergic synapses.作者: 山崩 時(shí)間: 2025-3-26 20:36
Hugh F. VanLandingham,Vladik Kreinovichor both platelet-derived and/or megakaryocyte-derived EVs. PDEVs can be isolated from blood or from isolated platelets after activation. In this chapter, we describe all commonly used PDEV isolation methods from blood and prepurified platelets.作者: 饑荒 時(shí)間: 2025-3-27 00:24 作者: affect 時(shí)間: 2025-3-27 02:29 作者: Concerto 時(shí)間: 2025-3-27 05:44 作者: antidote 時(shí)間: 2025-3-27 11:50
Methods to Analyze EVs,odologies to characterize nano-sized EVs have been developed. This chapter aims to summarize physical principles of novel and conventional technologies to be used in the EV field and to discuss advantages and limitations.作者: Heresy 時(shí)間: 2025-3-27 15:04 作者: chapel 時(shí)間: 2025-3-27 20:25
Multiplexed Phenotyping of Small Extracellular Vesicles Using Protein Microarray (EV Array),erform multiplexed phenotyping of EVs in an open platform. This protocol outlines the microarray printing procedure followed by the steps of capture and detection of small extracellular vesicles from plasma/serum or cell culture supernatants. The principles of data treatment and analysis are thoroughly described as well.作者: Triglyceride 時(shí)間: 2025-3-28 00:06 作者: 靦腆 時(shí)間: 2025-3-28 03:14 作者: 饑荒 時(shí)間: 2025-3-28 06:47
Cluster Analysis for Relational Data,ntercellular signalling become clearer, so does their potential utility as both diagnostic biomarkers for disease and as therapeutic vectors. Accurate assessment of exosomes, both their number and their cargo, is important for continued advancement in the field of vesicle research. To that end, seve作者: 繼而發(fā)生 時(shí)間: 2025-3-28 13:09
https://doi.org/10.1007/978-3-030-61282-5siopathological roles and their potential biomedical applications. However, the characterization and quantification of EVs constitute major challenges, mainly due to their small size and the lack of methods adapted for their study. Electron microscopy has made significant contributions to the EV fie作者: evasive 時(shí)間: 2025-3-28 17:25 作者: Coma704 時(shí)間: 2025-3-28 20:08 作者: Generic-Drug 時(shí)間: 2025-3-29 00:37
Fuzzy Multiple Attribute Decision Makingadigm. Recent studies have highlighted EVs carry preassembled complex biological information that elicit pleiotropic responses in target cells. It is well recognized that cells secrete essentially two EV subtypes that can be partially separated by differential centrifugation (DC): the larger size cl作者: CRACK 時(shí)間: 2025-3-29 03:06
Fuzzy Multiple Objective Decision Making,rom unpurified starting material in a high-throughput manner (J?rgensen et al., J Extracell vesicles 2:1–9, 2013). The technology was established to perform multiplexed phenotyping of EVs in an open platform. This protocol outlines the microarray printing procedure followed by the steps of capture a作者: Mitigate 時(shí)間: 2025-3-29 09:45 作者: 其他 時(shí)間: 2025-3-29 11:32
Fundamental Theorems in Fuzzy Normed Spaces,widely isolated and characterized from conditioned culture media and bodily fluids. The difficulty in isolating EV from tissues, however, has hindered their study in vivo. Here, we describe a novel method designed to isolate EV and characterize exosomes from the extracellular space of brain tissues.作者: Minuet 時(shí)間: 2025-3-29 17:42
Alexander Yazenin,Ilia Soldatenkoleased in the extracellular medium from cells when multivesicular bodies fuse with the plasma membrane, whereas microvesicles (i.e., shedding vesicles, 100 nm to 1 μm sized) bud from the plasma membrane. Exosomes and microvesicles carry functional proteins and nucleic acids (especially mRNAs and mic作者: 確定無(wú)疑 時(shí)間: 2025-3-29 22:41 作者: 配置 時(shí)間: 2025-3-30 01:35
Fuzzy Relation Equations with Words,ue class of extracellular organelles which play pivotal role in intercellular communication are conserved across prokaryotes and eukaryotes. Depending upon the cell origin and the functional state, the molecular cargo including proteins, lipids, and RNA within the EVs are modulated. Owing to this, E作者: 豐富 時(shí)間: 2025-3-30 04:41 作者: 舊石器時(shí)代 時(shí)間: 2025-3-30 09:19 作者: 能夠支付 時(shí)間: 2025-3-30 15:56
Multi-criteria Fuzzy Portfolio Optimization,y resolved on the flow cytometer available in their lab or facility. Although high-resolution flow cytometers are available and are being used for EV analysis, the use of these instruments for studying EVs requires careful use and validation by experienced small-particle flow cytometrists, beyond th作者: 嚴(yán)厲譴責(zé) 時(shí)間: 2025-3-30 19:16
978-1-4939-8284-4Springer Science+Business Media LLC 2017作者: Gyrate 時(shí)間: 2025-3-30 23:55
Exosomes and Microvesicles978-1-4939-6728-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Nutrient 時(shí)間: 2025-3-31 03:28 作者: entreat 時(shí)間: 2025-3-31 08:27 作者: Hyperalgesia 時(shí)間: 2025-3-31 09:55
https://doi.org/10.1007/978-1-4939-6728-5extracellular vesicles; cellular biology; cell-cell communication; electron microscopy; tunable resistan
