標(biāo)題: Titlebook: Exocytosis and Endocytosis; Methods and Protocol Florence Niedergang,Nicolas Vitale,Stéphane Gasman Book 2021 Springer Science+Business Med [打印本頁(yè)] 作者: 婉言 時(shí)間: 2025-3-21 16:53
書目名稱Exocytosis and Endocytosis影響因子(影響力)
書目名稱Exocytosis and Endocytosis影響因子(影響力)學(xué)科排名
書目名稱Exocytosis and Endocytosis網(wǎng)絡(luò)公開度
書目名稱Exocytosis and Endocytosis網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Exocytosis and Endocytosis被引頻次
書目名稱Exocytosis and Endocytosis被引頻次學(xué)科排名
書目名稱Exocytosis and Endocytosis年度引用
書目名稱Exocytosis and Endocytosis年度引用學(xué)科排名
書目名稱Exocytosis and Endocytosis讀者反饋
書目名稱Exocytosis and Endocytosis讀者反饋學(xué)科排名
作者: 延期 時(shí)間: 2025-3-21 23:08 作者: hypotension 時(shí)間: 2025-3-22 01:38 作者: 禮節(jié) 時(shí)間: 2025-3-22 06:54 作者: 常到 時(shí)間: 2025-3-22 09:22
https://doi.org/10.1007/978-3-658-00037-0ms, classified depending on critical proteins involved, speed, morphology of the derived intracellular vesicles, or substance trafficked. Pharmacological targeting of specific endocytosis pathways has a proven utility for diverse clinical applications from epilepsy to cancer. A multiplexable, high-c作者: 推遲 時(shí)間: 2025-3-22 13:03 作者: 推遲 時(shí)間: 2025-3-22 17:33
Felix Felleisen,Bj?rn Heidecke,Janis Sussick, the dominant mechanism of SV retrieval is activity-dependent bulk endocytosis (ADBE). Here, we describe a method to monitor ADBE in isolation from other SV endocytosis modes, via the uptake of large fluorescent fluid-phase markers in primary neuronal culture. Furthermore, we outline how to monitor作者: 得體 時(shí)間: 2025-3-22 21:44 作者: MUTE 時(shí)間: 2025-3-23 01:42
https://doi.org/10.1007/978-3-658-23345-7 However, with the advancement of secreted luciferase tools, it has become feasible for small laboratories to screen large numbers of compounds for effects on insulin secretion. The purpose of this chapter is to outline the methods involved in high-throughput screening for small molecules that chron作者: 修改 時(shí)間: 2025-3-23 07:59
Designing for Fun: User-Testing Case Studiesion and vesiculation of the sperm plasma membrane and outer acrosomal membrane, releasing the acrosomal content. Given the importance of the acrosome secretion in fertilization, many different methods have been developed to detect the acrosome reaction of sperm. In this chapter, we describe detailed作者: Benign 時(shí)間: 2025-3-23 09:44 作者: MEAN 時(shí)間: 2025-3-23 14:20
Furniere — Sperrholz Schichtholz are genetically manipulated by transient transfections provide a powerful tool for deciphering the underlying mechanisms of mast cell exocytosis. However, common methods to study regulated exocytosis in bulk culture of mast cells suffer from the drawback of high signal-to-noise ratio because of the作者: 死亡率 時(shí)間: 2025-3-23 19:43
Furniere — Sperrholz Schichtholzoped super-resolution microscopy technologies have generated unparalleled opportunities to help understand the molecular mechanisms regulating neutrophil vesicular trafficking, exocytosis, and associated functions at the molecular level. Here, we describe super-resolution and total internal reflecti作者: 極力證明 時(shí)間: 2025-3-24 01:05 作者: 外貌 時(shí)間: 2025-3-24 06:18 作者: Dysarthria 時(shí)間: 2025-3-24 08:25
Production Flow in Furniture Manufacturing,e role of specific proteins and reactions in this cascade requires a measurement method with high temporal resolution. Patch-clamp recordings of cell membrane capacitance in combination with calcium measurements, calcium uncaging, and carbon-fiber amperometry allow for the accurate determination of 作者: 使服水土 時(shí)間: 2025-3-24 11:46 作者: Digitalis 時(shí)間: 2025-3-24 17:59
https://doi.org/10.1007/978-1-0716-1044-2Amperometry; SNARE; Proteomics; Clathrin; flow cytometry; macropinocytosis作者: Encoding 時(shí)間: 2025-3-24 20:58 作者: 聯(lián)想記憶 時(shí)間: 2025-3-25 03:10
Exocytosis and Endocytosis978-1-0716-1044-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: ALLAY 時(shí)間: 2025-3-25 05:29
Florence Niedergang,Nicolas Vitale,Stéphane GasmanIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 透明 時(shí)間: 2025-3-25 09:23 作者: Enervate 時(shí)間: 2025-3-25 13:15
Book 2021ent pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins involved in exocytosis, specific protocols for exocytosis in specialized cells such as neutrophils or neuroendocrine cells, as well as secretion of e作者: 無(wú)脊椎 時(shí)間: 2025-3-25 19:49 作者: 微塵 時(shí)間: 2025-3-25 21:52 作者: 他很靈活 時(shí)間: 2025-3-26 00:53 作者: fibula 時(shí)間: 2025-3-26 06:08 作者: 愛管閑事 時(shí)間: 2025-3-26 10:50
Stoichiometry of Receptors at the Plasma Membrane During Their Endocytosis Using Total Internal Reflg a GFP-tagged receptor in a physiological relevant environment, a calibration technique for single-molecule analysis of GFP, and detection and tracking with an open-source software. This method allows to determine the number of receptor molecules at the plasma membrane in real time.作者: START 時(shí)間: 2025-3-26 13:19 作者: 哀悼 時(shí)間: 2025-3-26 17:43 作者: Isolate 時(shí)間: 2025-3-26 22:46 作者: CANON 時(shí)間: 2025-3-27 01:12 作者: anchor 時(shí)間: 2025-3-27 09:04 作者: 親屬 時(shí)間: 2025-3-27 11:03
Super-Resolution Microscopy and Particle-Tracking Approaches for the Study of Vesicular Trafficking hil vesicular trafficking, exocytosis, and associated functions at the molecular level. Here, we describe super-resolution and total internal reflection fluorescence (TIRF) microscopy approaches for the analysis of vesicular trafficking and associated functions of primary neutrophils.作者: BROTH 時(shí)間: 2025-3-27 15:00
An Approach to Monitor Exocytosis in White Adipocytesion of cells (SVF) within adipose tissue and how to differentiate SVF and cultured 3T3-L1 cells into adipocytes suitable for patch-clamp studies. We also give detailed protocols of how to record and analyze exocytosis in the differentiated cells.作者: acheon 時(shí)間: 2025-3-27 19:09
https://doi.org/10.1007/978-3-658-23345-7ariability in the assay. Using this approach in 384-well format, at least 48 and as many as 144 plates can theoretically be processed per week. This protocol serves as a guideline and can be modified as required for alternate stimulation paradigms and improved upon as new technologies become available.作者: 發(fā)展 時(shí)間: 2025-3-28 01:25
,Coomologia Sulle Varieta’ Complesse, I.,ay based on the successive transformation of adrenaline into adrenochrome and adrenolutine for fluorescence measurements. We also provide some guidelines for efficient cell recovery and for the use of this assay in the laboratory.作者: CODA 時(shí)間: 2025-3-28 02:22 作者: 溺愛 時(shí)間: 2025-3-28 07:10
High-Throughput Screening for Insulin Secretion Modulatorsariability in the assay. Using this approach in 384-well format, at least 48 and as many as 144 plates can theoretically be processed per week. This protocol serves as a guideline and can be modified as required for alternate stimulation paradigms and improved upon as new technologies become available.作者: 反感 時(shí)間: 2025-3-28 13:25
Bovine Chromaffin Cells: Culture and Fluorescence Assay for Secretionay based on the successive transformation of adrenaline into adrenochrome and adrenolutine for fluorescence measurements. We also provide some guidelines for efficient cell recovery and for the use of this assay in the laboratory.作者: jungle 時(shí)間: 2025-3-28 15:47 作者: expeditious 時(shí)間: 2025-3-28 22:44
Funktionssysteme der Gesellschaftteins that have reached the Golgi apparatus via the retrograde route from those that have not. The choice of antibodies or natural ligands allows to adjust these methods to virtually any chosen biological system.作者: MITE 時(shí)間: 2025-3-29 00:24
Designing for Fun: User-Testing Case Studiesscopy. Further, we discuss the level of complexity, costs, and the reasons why a researcher should choose each technique..This chapter is designed to provide the user with sufficient background to measure acrosomal exocytosis in human sperm.作者: Delectable 時(shí)間: 2025-3-29 05:11
https://doi.org/10.1007/978-3-030-57835-0ombination with selective protein immobilization (SPI). SPI is based on the coating of coverslips with anti-green fluorescent protein (GFP) antibodies, which capture the GFP-tagged RUSH cargos once exposed to the cell surface after its release by the addition of biotin.作者: MUT 時(shí)間: 2025-3-29 10:25 作者: PALMY 時(shí)間: 2025-3-29 14:46 作者: dagger 時(shí)間: 2025-3-29 19:37 作者: 偶然 時(shí)間: 2025-3-29 22:34 作者: 極微小 時(shí)間: 2025-3-30 00:23
https://doi.org/10.1007/978-3-662-68720-8hat are applicable to study the internalization of flaviviruses; dextrans; transporters, such as, glutamate transporter vGlut1; and peptidergic signaling molecules, including atrial natriuretic peptide into astrocytes, the most heterogeneous neuroglial cells, which play a key homeostatic role in the central nervous system.作者: Boycott 時(shí)間: 2025-3-30 08:05 作者: floodgate 時(shí)間: 2025-3-30 09:28 作者: puzzle 時(shí)間: 2025-3-30 13:41
,Oberfl?chengl?ttung von Lagenh?lzern,ion of cells (SVF) within adipose tissue and how to differentiate SVF and cultured 3T3-L1 cells into adipocytes suitable for patch-clamp studies. We also give detailed protocols of how to record and analyze exocytosis in the differentiated cells.作者: 有毛就脫毛 時(shí)間: 2025-3-30 19:15 作者: 不適當(dāng) 時(shí)間: 2025-3-31 00:04 作者: 柔聲地說(shuō) 時(shí)間: 2025-3-31 04:41
Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocyticen widely exploited to study constitutive endocytosis or ligand-induced receptor endocytosis. Compensatory endocytosis is the mechanism by which components of secretory vesicles are retrieved after vesicle fusion with the plasma membrane in response to cell stimulation and a rise in intracellular ca作者: 遺留之物 時(shí)間: 2025-3-31 06:56 作者: 他姓手中拿著 時(shí)間: 2025-3-31 10:26
High-Content Drug Discovery Screening of Endocytosis Pathwaysms, classified depending on critical proteins involved, speed, morphology of the derived intracellular vesicles, or substance trafficked. Pharmacological targeting of specific endocytosis pathways has a proven utility for diverse clinical applications from epilepsy to cancer. A multiplexable, high-c作者: 上流社會(huì) 時(shí)間: 2025-3-31 16:03
Methods for Monitoring Endocytosis in Astrocytesnism linked to different types of endocytosed cargo, including pathogens; therefore, several approaches can be applied. Here, we describe techniques that are applicable to study the internalization of flaviviruses; dextrans; transporters, such as, glutamate transporter vGlut1; and peptidergic signal作者: Obsessed 時(shí)間: 2025-3-31 21:14
Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent D, the dominant mechanism of SV retrieval is activity-dependent bulk endocytosis (ADBE). Here, we describe a method to monitor ADBE in isolation from other SV endocytosis modes, via the uptake of large fluorescent fluid-phase markers in primary neuronal culture. Furthermore, we outline how to monitor作者: conspicuous 時(shí)間: 2025-4-1 01:03
Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretionensitive to changes in cell number, and a significant amount of effort has to be spent to overcome this. We have developed a panel of quantitative flow cytometry-based assays and reporter cell lines that can be used to measure constitutive secretion. These assays are insensitive to changes in cell n
