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標(biāo)題: Titlebook: Exocytosis Methods; Peter Thorn Book 2014 Springer Science+Business Media New York 2014 Exocytosis control.Hormone release.Imaging.Microsc [打印本頁]

作者: 技巧    時間: 2025-3-21 17:17
書目名稱Exocytosis Methods影響因子(影響力)




書目名稱Exocytosis Methods影響因子(影響力)學(xué)科排名




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書目名稱Exocytosis Methods網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Exocytosis Methods被引頻次




書目名稱Exocytosis Methods被引頻次學(xué)科排名




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書目名稱Exocytosis Methods年度引用學(xué)科排名




書目名稱Exocytosis Methods讀者反饋




書目名稱Exocytosis Methods讀者反饋學(xué)科排名





作者: 牢騷    時間: 2025-3-21 20:15

作者: Expurgate    時間: 2025-3-22 02:31

作者: 憤慨點吧    時間: 2025-3-22 07:17
Funktionelle Pathologie in der Chirurgieated with perinatal lethality. More recent methods to study exocytosis specifically in the intact tissues include polar tracers and lipophilic dyes that can be used to directly visualize exocytosis with multiphoton excitation microscopy. A hallmark of the microscopic approach is that it offers posit
作者: 翻布尋找    時間: 2025-3-22 08:52

作者: mighty    時間: 2025-3-22 14:49
0893-2336 e the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field..978-1-4939-6329-4978-1-62703-676-4Series ISSN 0893-2336 Series E-ISSN 1940-6045
作者: mighty    時間: 2025-3-22 18:51

作者: 漂泊    時間: 2025-3-22 22:57
Imaging of Insulin Exocytosis from Pancreatic Beta Cells granules exist as large–dense core structures that are discernible by electron microscopy as an electron-dense interior surrounded by a clear region in a 300–350-nm intracellular membrane-delineated compartment (Greider et al., J Cell Biol 41:162–166, 1969; Lange, J Ultrastruct Res 46:301–307, 1974
作者: 極為憤怒    時間: 2025-3-23 04:21

作者: 制度    時間: 2025-3-23 06:10
Rapid Analysis of Synaptic Vesicle Endocytosis in Synaptosomessured by the uptake of the fluorescent styryl dye FM4-64. The synaptosomes may be used fresh or frozen and stored in liquid nitrogen, with the latter option greatly expanding the utility of the assay, by reducing the animal numbers required and allowing experiments to be done “on demand.” The assay
作者: Defense    時間: 2025-3-23 12:07

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作者: FEAS    時間: 2025-3-23 22:44
Isolated Neurohypophysial Terminals: Model for Depolarization–Secretion Couplingcitance and amperometric measurements. We also present evidence which show that the isolated neurohypophysial nerve terminals represent a powerful tool for studying the mechanisms underlying depolarization–secretion coupling (D-SC).
作者: enhance    時間: 2025-3-24 04:19
Funktionelle Elektrostimulation (ALEX). Eliminating the need for surface immobilization of vesicles, ALEX discriminates docked and fused vesicles in solution and measures their kinetics. We describe the principle, the experimental details, and the applications of ALEX to fusion studies.
作者: narcissism    時間: 2025-3-24 08:04
,Zyklische Ver?nderungen am Uterus,cretion. Our goal is to provide the reader with guidelines on how to apply both qualitative and quantitative intravital microscopy to study regulated exocytosis and to highlight advantages and limitations of this approach.
作者: 禁令    時間: 2025-3-24 11:06

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作者: eardrum    時間: 2025-3-24 19:32
Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Licretion. Our goal is to provide the reader with guidelines on how to apply both qualitative and quantitative intravital microscopy to study regulated exocytosis and to highlight advantages and limitations of this approach.
作者: 釋放    時間: 2025-3-25 00:25
The Sea Urchin Egg and Cortical Vesicles as Model Systems to Dissect the Fast, Ca2+-Triggered Steps this process. Here we describe detailed methods to isolate these native secretory vesicles, monitor the fusion process, assess the minimal essential biochemical components, and identify their ultrastructural interactions that define the triggered exocytotic pathway.
作者: 夸張    時間: 2025-3-25 05:16
Book 2014he field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission.? The book begins with a section covering a range of techniques being applied to the study of single-vesicle fusion events, which are key in order to gain insig
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作者: 擋泥板    時間: 2025-3-26 05:11
Peter ThornIncludes cutting-edge techniques and methods for studying exocytosis in the brain.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
作者: Metamorphosis    時間: 2025-3-26 09:33
Neuromethodshttp://image.papertrans.cn/e/image/318626.jpg
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作者: 辮子帶來幫助    時間: 2025-3-26 20:35
Funktionelle Elektrostimulation in the exocytosis. In vitro fusion assays using reconstituted proteoliposomes have been widely used for studying the functions of the SNARE proteins as well as those of the regulatory factors. Recently, single-vesicle assays have provided mechanisms of fusion and their dynamic features in unprecede
作者: 陶醉    時間: 2025-3-27 00:25

作者: clarify    時間: 2025-3-27 03:07
https://doi.org/10.1007/978-3-662-07346-9 many significant biological pathways. These molecules, including neurotransmitters, hormones, growth factors, and peptides, are released from cells via a process called exocytosis. Our laboratory has utilized a noninvasive method of measuring the release of oxidizable molecules from cells, known as
作者: SHOCK    時間: 2025-3-27 07:37
https://doi.org/10.1007/978-3-662-68025-4s at the level of exocytosis of insulin, transcriptional rate of the insulin gene, translation of the mRNA, and processing of the proinsulin to mature insulin (Mahato et al., Biochem J 174:517–526, 1978; Hedeskov, Physiol Rev 60:442–509, 1980; Welsh et al., J Biol Chem 260:13590–13594, 1985; Welsh e
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作者: 天真    時間: 2025-3-27 17:26
https://doi.org/10.1007/978-3-662-25758-6 which reflect membrane area changes due to fusion or fission of secretory vesicles. This parameter can be monitored directly by the electrophysiologic patch-clamp technique developed by Erwin Neher and Bert Sakmann in 1976. It is still widely used, primarily for the study of currents across ion cha
作者: 搬運工    時間: 2025-3-27 21:41
Funktionelle Pathologie in der Chirurgiedescription of a selection of the methods that have been adapted to study regulated exocytosis in endocrine tissue slices is provided in this chapter. Such adaptation of the methods has been associated with a plethora of open issues that required innovative solutions, but innovation has been and sha
作者: Epithelium    時間: 2025-3-28 00:21
,Zyklische Ver?nderungen am Uterus, recent application of intravital microscopy to image subcellular structures in vivo has enabled researchers to investigate the machinery controlling regulated exocytosis in live rodents. Here, we describe selected experimental models that have been used to investigate the dynamics of the secretory
作者: Aesthete    時間: 2025-3-28 02:59
Neuromuscular Dysfunction and Paraplegia,lecules that target SVE may prove to be useful not only for basic research into synaptic function but also in treating diseases that involve pathological neurotransmission. However, there are relatively few small molecules targeting SVE that have known molecular targets, and the development of these
作者: dandruff    時間: 2025-3-28 07:17
https://doi.org/10.1007/3-211-30826-1ituitaries). These nerve terminals release the two neuropeptides, oxytocin and vasopressin, which are easily assayed by radioimmunoassay and/or ELISA. Depolarization-induced exocytosis is dependent on the same parameters as those regulating release from the whole, intact neurohypophysis. The isolate
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作者: 窩轉(zhuǎn)脊椎動物    時間: 2025-3-29 03:15

作者: Frenetic    時間: 2025-3-29 11:05
Carbon-Fiber Amperometry in the Study of Exocytosis many significant biological pathways. These molecules, including neurotransmitters, hormones, growth factors, and peptides, are released from cells via a process called exocytosis. Our laboratory has utilized a noninvasive method of measuring the release of oxidizable molecules from cells, known as
作者: 改正    時間: 2025-3-29 12:03

作者: BIDE    時間: 2025-3-29 19:12

作者: 地名詞典    時間: 2025-3-29 21:47
Electrophysiologic Measurements of Membrane Capacitance in Hormone-Secreting Cells which reflect membrane area changes due to fusion or fission of secretory vesicles. This parameter can be monitored directly by the electrophysiologic patch-clamp technique developed by Erwin Neher and Bert Sakmann in 1976. It is still widely used, primarily for the study of currents across ion cha
作者: Nibble    時間: 2025-3-30 00:21
Measuring Exocytosis in Endocrine Tissue Slicesdescription of a selection of the methods that have been adapted to study regulated exocytosis in endocrine tissue slices is provided in this chapter. Such adaptation of the methods has been associated with a plethora of open issues that required innovative solutions, but innovation has been and sha
作者: mucous-membrane    時間: 2025-3-30 04:14
Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Li recent application of intravital microscopy to image subcellular structures in vivo has enabled researchers to investigate the machinery controlling regulated exocytosis in live rodents. Here, we describe selected experimental models that have been used to investigate the dynamics of the secretory




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