標(biāo)題: Titlebook: Ewing Sarcoma; Methods and Protocol Florencia Cidre-Aranaz,Thomas G. P. Grünewald Book 2021 Springer Science+Business Media, LLC, part of S [打印本頁] 作者: Forestall 時(shí)間: 2025-3-21 17:26
書目名稱Ewing Sarcoma影響因子(影響力)
書目名稱Ewing Sarcoma影響因子(影響力)學(xué)科排名
書目名稱Ewing Sarcoma網(wǎng)絡(luò)公開度
書目名稱Ewing Sarcoma網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Ewing Sarcoma被引頻次
書目名稱Ewing Sarcoma被引頻次學(xué)科排名
書目名稱Ewing Sarcoma年度引用
書目名稱Ewing Sarcoma年度引用學(xué)科排名
書目名稱Ewing Sarcoma讀者反饋
書目名稱Ewing Sarcoma讀者反饋學(xué)科排名
作者: atopic-rhinitis 時(shí)間: 2025-3-21 23:10 作者: Bureaucracy 時(shí)間: 2025-3-22 03:25 作者: 專心 時(shí)間: 2025-3-22 05:06
Florencia Cidre-Aranaz,Thomas G. P. GrünewaldIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: insolence 時(shí)間: 2025-3-22 09:43 作者: 斷言 時(shí)間: 2025-3-22 14:33
978-1-0716-1022-0Springer Science+Business Media, LLC, part of Springer Nature 2021作者: 斷言 時(shí)間: 2025-3-22 18:08
Ewing Sarcoma978-1-0716-1020-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 相符 時(shí)間: 2025-3-22 22:52
Russell Wanhill,Simon Barter,Loris Molent date, genetic investigations have identified key somatic mutations and germline variants of importance for EwS risk. While substantial progress is being made in uncovering the genetic etiology of EwS, considerable gaps in knowledge remain. Herein, we provide a summary of methodological approaches f作者: 疾馳 時(shí)間: 2025-3-23 05:12
Fatigue Crack Growth in Rubber Materialsion about the level of protein expression in different cell conditions, in comparison with other tissues or upon induced molecular changes. Based on the specific pattern of protein expression of the tissue, as well as on the characteristics of the protein of interest, the antibodies and protocol of 作者: HEW 時(shí)間: 2025-3-23 06:22 作者: HAIRY 時(shí)間: 2025-3-23 10:07
Erdogan Madenci,Ibrahim Guven,Bahattin Kiliceased from tumor cells or in response to tumor growth can be used for quantification of the tumor burden. However, only a fraction of pediatric tumors has none of these serum markers, but tumor-specific genetic alterations represent reliable alternatives. Here we describe a method for using genomic 作者: nutrition 時(shí)間: 2025-3-23 14:34
https://doi.org/10.1007/978-4-431-73464-2However, the first steps of the diagnostic process are crucial to achieve the maximum possible diagnostic performance. In this chapter we will review how to handle the diagnostic specimen from its collection, how to prepare it for diagnosis, how to make a complete pathology report, and provide guida作者: Embolic-Stroke 時(shí)間: 2025-3-23 19:38 作者: Arthritis 時(shí)間: 2025-3-24 00:06 作者: HARD 時(shí)間: 2025-3-24 05:15
https://doi.org/10.1007/978-3-319-56227-8a variety of chimeric genes. Reference laboratories are increasingly implementing NGS-based techniques to overcome several limitations of conventional singleplex determinations. We have been early adopters of a targeted-RNA sequencing method based on Anchored multiplex PCR, which allows assessing se作者: 的是兄弟 時(shí)間: 2025-3-24 06:51
Uwe Zerbst,Mauro Madia,Carla Beckmann viral transduction have revolutionized the field of molecular biology and paved the ground for various gene-editing strategies such as TALEN, zinc finger nucleases, and ultimately CRISPR. In Ewing sarcoma (EwS), almost as many genes are repressed by the expression of EWSR1-FLI1 as are upregulated b作者: apiary 時(shí)間: 2025-3-24 11:52
Acoustic Emission and Barkhausen Pulses,f these DNA sequences is especially paramount for its main driver mutation is a fusion transcription factor that binds different motifs than its wild-type constituents. Here, we describe the process of analyzing the enhancer activity of regulatory DNA sequences using transfection-based dual-lucifera作者: 錯(cuò)誤 時(shí)間: 2025-3-24 18:19
https://doi.org/10.1007/978-3-031-13498-2ssion/cell division, cell death, and cellular senescence. Deregulation of cell proliferation is a key feature of cancer cells, making assessment of proliferation a central methodological issue in cancer research. Especially in Ewing sarcoma (EwS) that exhibit a high proliferative capacity, experimen作者: phytochemicals 時(shí)間: 2025-3-24 19:33 作者: Cloudburst 時(shí)間: 2025-3-24 23:36 作者: verdict 時(shí)間: 2025-3-25 05:50
The Minerals, Metals & Materials Seriest to obtain the cell-of-origin of Ewing sarcoma that is tolerant of . expression. Here we describe the generation of the .-expressing mouse model for Ewing sarcoma by selecting embryonic chondrogenic progenitor, eSZ cells that contain Ewing sarcoma precursors.作者: sclera 時(shí)間: 2025-3-25 09:20
Raghavan Srinivasan,M. Ashraf Imamppropriate animal models for Ewing sarcoma, subcutaneous murine xenograft models currently offer the simplest way to investigate antineoplastic effects of therapeutics or biological functions of target genes in vivo. In order to properly carry out tumor growth analysis via subcutaneous xenografts of作者: IRATE 時(shí)間: 2025-3-25 15:10
Germline Variation and Somatic Alterations in Ewing Sarcoma date, genetic investigations have identified key somatic mutations and germline variants of importance for EwS risk. While substantial progress is being made in uncovering the genetic etiology of EwS, considerable gaps in knowledge remain. Herein, we provide a summary of methodological approaches f作者: 使服水土 時(shí)間: 2025-3-25 16:26 作者: Veneer 時(shí)間: 2025-3-25 21:04 作者: 愛哭 時(shí)間: 2025-3-26 02:19 作者: Measured 時(shí)間: 2025-3-26 07:06 作者: 大罵 時(shí)間: 2025-3-26 09:39
Molecular Approaches to Diagnosis in Ewing Sarcoma: Fluorescence In Situ Hybridization (FISH)and molecular features. Though the implementation of broad RNA sequencing in diagnostic molecular pathology routines has substantially changed the standards of molecular affirmation of diagnoses, fluorescence in situ hybridization (FISH) on formalin-fixed, paraffin-embedded (FFPE) tissue sections is作者: Formidable 時(shí)間: 2025-3-26 13:06
Molecular Approaches to Diagnosis in Ewing Sarcoma: RT-PCRations that give rise to fusion transcripts that codify for aberrant transcription factors. More than 95% of Ewing sarcoma harbor translocations that produce the fusion of the EWSR1 gene with the transcription factors FLI1 or ERG. This feature can be used to diagnose this entity unambiguously..In th作者: gregarious 時(shí)間: 2025-3-26 17:14
Molecular Approaches to Diagnosis in Ewing Sarcoma: Targeted RNA Sequencinga variety of chimeric genes. Reference laboratories are increasingly implementing NGS-based techniques to overcome several limitations of conventional singleplex determinations. We have been early adopters of a targeted-RNA sequencing method based on Anchored multiplex PCR, which allows assessing se作者: 我不重要 時(shí)間: 2025-3-26 23:19 作者: 引起 時(shí)間: 2025-3-27 04:32 作者: macular-edema 時(shí)間: 2025-3-27 08:55 作者: 撤退 時(shí)間: 2025-3-27 11:05 作者: fidelity 時(shí)間: 2025-3-27 15:55 作者: Extort 時(shí)間: 2025-3-27 19:32 作者: armistice 時(shí)間: 2025-3-27 22:33
Tumor Growth Analysis of Ewing Sarcoma Cell Lines Using Subcutaneous Xenografts in Miceppropriate animal models for Ewing sarcoma, subcutaneous murine xenograft models currently offer the simplest way to investigate antineoplastic effects of therapeutics or biological functions of target genes in vivo. In order to properly carry out tumor growth analysis via subcutaneous xenografts of作者: 是剝皮 時(shí)間: 2025-3-28 03:30 作者: Ingrained 時(shí)間: 2025-3-28 10:00 作者: 轉(zhuǎn)換 時(shí)間: 2025-3-28 12:47 作者: 動(dòng)物 時(shí)間: 2025-3-28 18:09
Uwe Zerbst,Mauro Madia,Carla Beckmannnal) reexpression of a gene of interest. We provide an extensive instruction on molecular cloning, plasmid generation, viral transduction, and expression validation. Finally, we address common problems and highlight potential pitfalls, which can easily be avoided by thoughtful guidance.作者: 咆哮 時(shí)間: 2025-3-28 18:44 作者: Employee 時(shí)間: 2025-3-29 02:06 作者: 翻布尋找 時(shí)間: 2025-3-29 04:34 作者: 令人發(fā)膩 時(shí)間: 2025-3-29 10:44 作者: Dysplasia 時(shí)間: 2025-3-29 13:08 作者: 沐浴 時(shí)間: 2025-3-29 16:48 作者: overreach 時(shí)間: 2025-3-29 23:36 作者: 原來 時(shí)間: 2025-3-30 01:30
https://doi.org/10.1007/978-4-431-73464-2how to handle the diagnostic specimen from its collection, how to prepare it for diagnosis, how to make a complete pathology report, and provide guidance for the reasonable use of immunohistochemical techniques in this malignancy.作者: Fecal-Impaction 時(shí)間: 2025-3-30 05:22 作者: 同位素 時(shí)間: 2025-3-30 09:48
Russell Wanhill,Simon Barter,Loris Molentor future genomic investigations of EwS. We anticipate this recommended analytical framework for germline and somatic investigations, along with genomic data from growing EwS case series, will aid in accelerating new genomic discoveries in EwS and expand knowledge of the genetic architecture of EwS.作者: dithiolethione 時(shí)間: 2025-3-30 14:51
Fatigue and Fracture Reliability Engineeringis chapter we describe a RT-PCR method that allows for the detection of the most frequent alterations with elevated specificity and sensitivity which is able to distinguish among the different types of fusions. The method is fast and economical, and can be carried out with the conventional equipment available in any molecular biology laboratory.作者: 種族被根除 時(shí)間: 2025-3-30 20:06
https://doi.org/10.1007/978-3-319-56227-8veral fusion transcripts simultaneously with previous knowledge of only one partner gene. Here we describe in detail our protocol and tips for nucleic acid extraction, library preparation, sequencing, and reporting of gene fusions.作者: 不幸的人 時(shí)間: 2025-3-30 21:48 作者: acclimate 時(shí)間: 2025-3-31 04:56
https://doi.org/10.1007/978-3-031-13498-2tal assessment of proliferation in preclinical research plays an important role. Among the variety of applicable methods, trypan blue exclusion is described here as a robust, easy-to-perform, and cost-effective method to assess cell proliferation in an experimental setting.作者: 索賠 時(shí)間: 2025-3-31 05:12 作者: 預(yù)兆好 時(shí)間: 2025-3-31 12:50 作者: 植物學(xué) 時(shí)間: 2025-3-31 15:52 作者: harbinger 時(shí)間: 2025-3-31 18:11 作者: 誘惑 時(shí)間: 2025-3-31 22:06
Analysis of Regulatory DNA Sequences by Dual-Luciferase Reporter Assays in Ewing Sarcomase reporter assays in Ewing sarcoma cell lines. To this end, we provide a protocol for cloning sequences of interest from genomic DNA into a firefly luciferase-containing plasmid, transfecting Ewing sarcoma cells with plasmids and measuring luciferase expression by luminescence. The entire procedure can be completed in 14?days.
