標(biāo)題: Titlebook: Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation; Narendra Wajapeyee,Romi Gupta Book 2017 Springer Science+B [打印本頁] 作者: emanate 時(shí)間: 2025-3-21 20:03
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation影響因子(影響力)
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation影響因子(影響力)學(xué)科排名
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation網(wǎng)絡(luò)公開度
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation被引頻次
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation被引頻次學(xué)科排名
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation年度引用
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation年度引用學(xué)科排名
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation讀者反饋
書目名稱Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation讀者反饋學(xué)科排名
作者: 欲望小妹 時(shí)間: 2025-3-21 23:52 作者: clarify 時(shí)間: 2025-3-22 01:22 作者: 擁護(hù) 時(shí)間: 2025-3-22 05:11 作者: Habituate 時(shí)間: 2025-3-22 09:23 作者: 罵人有污點(diǎn) 時(shí)間: 2025-3-22 12:55
High-Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeor template for revealing short-term changes in gene expression, alterations in RNA decay rates, and the kinetics of RNA processing as well as the differentiation thereof. Here, we describe the metabolic labeling and purification of newly transcribed RNA with 4-thiouridine, by which these limitations are overcome.作者: 罵人有污點(diǎn) 時(shí)間: 2025-3-22 18:49
Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in ,,clude detailed methods for growing yeast cells, total RNA isolation, and Northern blotting. Although the chapter focuses on NMD, the methods can be easily adapted to assess the effect of other mRNA decay pathways.作者: chance 時(shí)間: 2025-3-23 01:03 作者: 飾帶 時(shí)間: 2025-3-23 03:20
https://doi.org/10.1007/b110207oliferation, apoptosis, DNA repair, and cell cycle. Therefore, unveiling the mechanisms of eIF3 action in tumorigenesis may help identify attractive targets for cancer therapy. Here, we describe a series of methods used in the study of eIF3 function in regulating protein synthesis, tumorigenesis, and cellular response to therapeutic treatments.作者: 溫和女人 時(shí)間: 2025-3-23 08:07
Alexander M. Polyakov,Fedor K. Popovmutant fungal species. The proteoforms exhibiting differential abundances can be subjected to further targeted studies by other MS or orthogonal (e.g., biochemical) assays. This method can be generally adapted for screening of changes in histone modifications between samples such as wild type vs. mutant or healthy vs. diseased.作者: ABOUT 時(shí)間: 2025-3-23 12:56 作者: humectant 時(shí)間: 2025-3-23 15:35 作者: ANTI 時(shí)間: 2025-3-23 20:25
https://doi.org/10.1007/978-0-387-75843-5ther cell types. We describe here a detailed protocol for the isolation of highly purified endocrine cell subtypes (beta, alpha, and delta cells) from human cadaveric islets, to perform cell-type-specific epigenomic analysis of histone modifications as well as global gene expression profiling.作者: Acupressure 時(shí)間: 2025-3-23 22:55 作者: fiction 時(shí)間: 2025-3-24 03:27
Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets,ther cell types. We describe here a detailed protocol for the isolation of highly purified endocrine cell subtypes (beta, alpha, and delta cells) from human cadaveric islets, to perform cell-type-specific epigenomic analysis of histone modifications as well as global gene expression profiling.作者: Employee 時(shí)間: 2025-3-24 10:27
Book 2017y-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,?.Eukaryotic Transcription and Post-Transcription Gene Expression Regulation?.aims to ensure successful results in the further study of this vital field..作者: 誤傳 時(shí)間: 2025-3-24 12:48
The , as Transcendental Phenomenologyoid, dependence on creatine phosphate. Important reaction components needed for optimal processing are discussed. We also provide an optimized procedure for preparing small-scale HeLa nuclear extracts from adherent cells for use in 3′ cleavage in vitro.作者: 休戰(zhàn) 時(shí)間: 2025-3-24 15:27
https://doi.org/10.1057/9780230283978 stable human embryonic stem cell line containing both Sp-dCas9-KRAB and sgRNA, followed by inducible expression of Sp-dCas9-KRAB to analyze functional effects of dCas9-KRAB at target loci in human embryonic stem cells.作者: 檔案 時(shí)間: 2025-3-24 20:43 作者: Statins 時(shí)間: 2025-3-25 02:29
Using an Inducible CRISPR-dCas9-KRAB Effector System to Dissect Transcriptional Regulation in Human stable human embryonic stem cell line containing both Sp-dCas9-KRAB and sgRNA, followed by inducible expression of Sp-dCas9-KRAB to analyze functional effects of dCas9-KRAB at target loci in human embryonic stem cells.作者: 半身雕像 時(shí)間: 2025-3-25 05:25 作者: 陳腐思想 時(shí)間: 2025-3-25 07:56 作者: Pigeon 時(shí)間: 2025-3-25 15:24
Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry,mutant fungal species. The proteoforms exhibiting differential abundances can be subjected to further targeted studies by other MS or orthogonal (e.g., biochemical) assays. This method can be generally adapted for screening of changes in histone modifications between samples such as wild type vs. mutant or healthy vs. diseased.作者: 幼稚 時(shí)間: 2025-3-25 16:07
Large-Scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressoror suppressor gene, using RASSF1A as an example. The approach we describe is a general RNAi screening strategy that can be applied to identify other factors that drive and/or maintain epigenetic modifications on specific genes, including cancer-related genes.作者: 水獺 時(shí)間: 2025-3-25 22:47 作者: AORTA 時(shí)間: 2025-3-26 01:00 作者: 集聚成團(tuán) 時(shí)間: 2025-3-26 05:40 作者: 舔食 時(shí)間: 2025-3-26 10:13 作者: TOXIN 時(shí)間: 2025-3-26 13:54
Systematic Discovery of Chromatin-Bound Protein Complexes from ChIP-seq Datasets,r, transcription factors rarely bind chromatin alone but often bind together with other cofactors, forming protein complexes. Here, we describe a computational method that integrates multiple ChIP-seq and RNA-seq datasets to discover protein complexes and determine their role as activators or repres作者: fructose 時(shí)間: 2025-3-26 20:46 作者: 合乎習(xí)俗 時(shí)間: 2025-3-26 23:42
Establishment of Time- and Cell-Specific RNAi in ,,cts of genes on various life phenomena. In particular, RNAi is a powerful tool that enables time- or cell-specific knockdown via heat shock-inducible RNAi or cell-specific RNAi. However, the conventional RNAi methods are insufficient for investigating pleiotropic genes with various sites of action a作者: debris 時(shí)間: 2025-3-27 01:25
Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing,nsists of two components: the Cas9 protein and a guide RNA. To date, delivery of these two components has been achieved using either plasmid or viral vectors or direct delivery of protein and RNA. Plasmid- and virus-free direct delivery of Cas9 protein and guide RNA has several advantages over the c作者: Outwit 時(shí)間: 2025-3-27 08:52 作者: Servile 時(shí)間: 2025-3-27 12:31
eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response,ost complex initiation factor consisting of 13 putative subunits. A growing number of studies suggest that eIF3 and its subunits may represent a new group of proto-oncogenes and associates with prognosis. They regulate translation of a subset of mRNAs involved in many cellular processes including pr作者: 尾隨 時(shí)間: 2025-3-27 17:17
High-Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Laben, transport), and degradation. Profiling these changes provides valuable information on the regulation of gene expression. Total cellular RNA is a poor template for revealing short-term changes in gene expression, alterations in RNA decay rates, and the kinetics of RNA processing as well as the dif作者: Senescent 時(shí)間: 2025-3-27 18:05
Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry,odifications (i.e., histone code). In this protocol, we describe a top-down workflow that employs liquid chromatography (LC) coupled to mass spectrometry (MS), for fast global profiling of changes in histone proteoforms, and apply LCMS top-down approach for comparative analysis of a wild-type and a 作者: forthy 時(shí)間: 2025-3-27 23:20 作者: Intuitive 時(shí)間: 2025-3-28 03:46
,Optimizing In Vitro Pre-mRNA 3′ Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HNA 3′ cleavage reaction, which defines the downstream end of the 3′ untranslated region and, in nearly all mRNA, prepares the message for addition of the poly(A) tail. The in vitro reconstitution of 3′ cleavage provides an experimental means to investigate the roles of the various multi-subunit clea作者: 腐敗 時(shí)間: 2025-3-28 09:08
Unbiased Interrogation of 3D Genome Topology Using Chromosome Conformation Capture Coupled to High-somes are folded in three-dimensional (3D) space. 3C and its derivatives have contributed tremendously to the now widely accepted view that genome topology plays an important role in many major cellular processes, at a chromosome-wide scale, but certainly also at the level of individual genetic loci作者: PET-scan 時(shí)間: 2025-3-28 12:29
Using an Inducible CRISPR-dCas9-KRAB Effector System to Dissect Transcriptional Regulation in Humanlatory networks in pluripotency and potentially in differentiation intermediates of all three germ layers makes this a valuable tool for the stem cell community. Catalytically inactive Cas9 fused to transcriptional/chromatin effector domains allows for silencing or activation of a genomic region of 作者: Visual-Acuity 時(shí)間: 2025-3-28 18:09 作者: theta-waves 時(shí)間: 2025-3-28 19:21
Determination of Alternate Splicing Events Using Transcriptome Arrays,tes. High-throughput expression profiling analysis of splice variants has thus far been limited by sample requirements and an appropriate platform for quantitation and analysis. Here we describe Affymetrix GeneChip Human Transcriptome Array 2.0, which is employed for comprehensive examination of all作者: COW 時(shí)間: 2025-3-29 01:54
Large-Scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor provided the opportunity to identify factors and pathways involved in complex biological processes by performing unbiased loss-of-function screens on a genome-wide scale. Here we describe a genome-wide RNAi screening strategy to identify factors that regulates epigenetic silencing of a specific tum作者: 消瘦 時(shí)間: 2025-3-29 04:49 作者: 厚臉皮 時(shí)間: 2025-3-29 10:58
https://doi.org/10.1007/978-94-015-3963-0tion. Here we describe a TAP-tag purification of chromatin-bound proteins along with associated nucleosomes, which allow exhaustive identification of protein partners. Moreover, this method allows exhaustive identification of the post-translational modifications (PTMs) of the associated histones. Th作者: 荒唐 時(shí)間: 2025-3-29 12:13
https://doi.org/10.1007/978-3-030-04966-9ot of many chromatin features in vivo. These chromatin profiles are obtained by immunoprecipitation of cross-linked chromatin fragments to enrich the feature of interest. Sequencing and aligning the underlying DNA sequences to the genome make it possible to virtually reconstruct the global distribut作者: 征服 時(shí)間: 2025-3-29 17:53
Martin Buber and the Life of Dialoguer, transcription factors rarely bind chromatin alone but often bind together with other cofactors, forming protein complexes. Here, we describe a computational method that integrates multiple ChIP-seq and RNA-seq datasets to discover protein complexes and determine their role as activators or repres作者: GUILE 時(shí)間: 2025-3-29 22:40
https://doi.org/10.1057/9781403978516d nucleosome positioning. Genome-wide sequencing is performed utilizing adapter sequences inserted by a prokaryotic transposase, Tn5, into the accessible regions of chromatin. Here we describe the use of ATAC-seq in the zebrafish embryo and thereby the applicability of this approach in whole vertebr作者: Deject 時(shí)間: 2025-3-30 02:22
Communication in the Mathematics Classroom,cts of genes on various life phenomena. In particular, RNAi is a powerful tool that enables time- or cell-specific knockdown via heat shock-inducible RNAi or cell-specific RNAi. However, the conventional RNAi methods are insufficient for investigating pleiotropic genes with various sites of action a作者: 甜食 時(shí)間: 2025-3-30 04:48
https://doi.org/10.1007/978-1-4471-1731-5nsists of two components: the Cas9 protein and a guide RNA. To date, delivery of these two components has been achieved using either plasmid or viral vectors or direct delivery of protein and RNA. Plasmid- and virus-free direct delivery of Cas9 protein and guide RNA has several advantages over the c作者: antiandrogen 時(shí)間: 2025-3-30 10:16 作者: Arroyo 時(shí)間: 2025-3-30 15:20 作者: 寵愛 時(shí)間: 2025-3-30 17:48
https://doi.org/10.1057/9781137470690n, transport), and degradation. Profiling these changes provides valuable information on the regulation of gene expression. Total cellular RNA is a poor template for revealing short-term changes in gene expression, alterations in RNA decay rates, and the kinetics of RNA processing as well as the dif作者: 可卡 時(shí)間: 2025-3-30 23:37
Alexander M. Polyakov,Fedor K. Popovodifications (i.e., histone code). In this protocol, we describe a top-down workflow that employs liquid chromatography (LC) coupled to mass spectrometry (MS), for fast global profiling of changes in histone proteoforms, and apply LCMS top-down approach for comparative analysis of a wild-type and a 作者: 無禮回復(fù) 時(shí)間: 2025-3-31 03:43 作者: 恃強(qiáng)凌弱的人 時(shí)間: 2025-3-31 05:39 作者: confide 時(shí)間: 2025-3-31 09:27 作者: Indebted 時(shí)間: 2025-3-31 16:01 作者: 慷慨不好 時(shí)間: 2025-3-31 20:02
Thinking About Indigenous Legal Orderss often fine-tuned by epigenetic modifications, including DNA methylation and histone modifications. One such histone modification is ubiquitination that predominately occurs in mono-ubiquitinated forms on histone H2A and H2B. We recently identified and characterized a novel E3 ligase called TRIM37 作者: 證明無罪 時(shí)間: 2025-3-31 22:26 作者: 不容置疑 時(shí)間: 2025-4-1 03:07 作者: 催眠 時(shí)間: 2025-4-1 08:44 作者: 減至最低 時(shí)間: 2025-4-1 12:28
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/316450.jpg作者: SOBER 時(shí)間: 2025-4-1 17:49
