標(biāo)題: Titlebook: Estrogen Receptors; Methods and Protocol Kathleen M. Eyster Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under [打印本頁] 作者: 有作用 時(shí)間: 2025-3-21 19:44
書目名稱Estrogen Receptors影響因子(影響力)
書目名稱Estrogen Receptors影響因子(影響力)學(xué)科排名
書目名稱Estrogen Receptors網(wǎng)絡(luò)公開度
書目名稱Estrogen Receptors網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Estrogen Receptors被引頻次
書目名稱Estrogen Receptors被引頻次學(xué)科排名
書目名稱Estrogen Receptors年度引用
書目名稱Estrogen Receptors年度引用學(xué)科排名
書目名稱Estrogen Receptors讀者反饋
書目名稱Estrogen Receptors讀者反饋學(xué)科排名
作者: arthroplasty 時(shí)間: 2025-3-21 21:36
,Immunoblot Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 /GPERlational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immunoblotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estro作者: Offensive 時(shí)間: 2025-3-22 01:28 作者: Bombast 時(shí)間: 2025-3-22 06:47 作者: 兵團(tuán) 時(shí)間: 2025-3-22 10:53 作者: Innocence 時(shí)間: 2025-3-22 13:36
Quantification of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate in Responsls, protein–protein interaction, and protein modifications in cells and tissues. The proximity ligation assay (PLA), a method of detection that combines immunologic and PCR-based approaches, was developed to overcome some of the drawbacks that are inherent with other detection methods. The PLA allow作者: Innocence 時(shí)間: 2025-3-22 20:42 作者: Interlocking 時(shí)間: 2025-3-22 21:58
Extraction of RNA and Analysis of Estrogen-Responsive Genes by RT-qPCR, measuring estrogen receptor transcript levels as well as gene expression changes in response to estrogen stimulation as it is quick, accurate, and robust and allows the measurement of gene expression in a variety of tissues and cells. This chapter describes the protocols used for RNA extraction and作者: 諂媚于人 時(shí)間: 2025-3-23 05:13 作者: STENT 時(shí)間: 2025-3-23 05:35
The Use of ERE-Luc Reporter Mice to Monitor Estrogen Receptor Transcriptional Activity in a ,-Temposociated with the menopause still represents a sort of sought after the “Holy Grail.”.Nowadays, the combination of estrogens and selective estrogen receptor modulators (SERMs), a class of compounds with a mixed agonist/antagonistic activity on the estrogen receptor (ER) in various tissues, represent作者: Constant 時(shí)間: 2025-3-23 15:32 作者: MUTED 時(shí)間: 2025-3-23 21:51
An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of Estrogen Receptor Beta,en and activate transcription through direct or indirect interactions with DNA. Revealing their interactions with the chromatin is key to understanding their transcriptional activities and their biological functions. Chromatin-immunoprecipitation followed by sequencing (ChIP-Seq) is a powerful techn作者: 埋葬 時(shí)間: 2025-3-24 02:15 作者: Hyperlipidemia 時(shí)間: 2025-3-24 03:04
Anota2seq Analysis for Transcriptome-Wide Studies of mRNA Translation,n responses to intra- and extracellular signals. Moreover, dysregulated mRNA translation is a common feature in disease states, including neurological disorders and cancer. Yet, most studies of gene expression focus on analysis of mRNA levels, leaving variations in translational efficiencies largely作者: 尊嚴(yán) 時(shí)間: 2025-3-24 06:55
Whole-Genome Genotyping Using DNA Microarrays for Population Genetics,–31, 2015). Tools to measure genetic variation have matured significantly throughout this advancement in knowledge (Lenoir and Giannella. J Biomed Discov Collab 1:11, 2006; Marzancola et al. Methods Mol Biol 1368:161–178, 2016). In this chapter, the focus is on the laboratory methods developed to pe作者: sorbitol 時(shí)間: 2025-3-24 11:26
Proteomics Analysis of the Estrogen Effects in the Rat Uterus Using Gel-LC and Tandem Mass Spectroms, tissues, biological fluids, secretome, etc.) is a useful strategy to identify proteins and analyze their changes. Samples processed through a gel-free approach provide a simple method for protein separation and profile comparison of different conditions, such as using fewer steps in the protocol,作者: Arroyo 時(shí)間: 2025-3-24 17:29
Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells,nd can also function as biomarkers. Here, we describe a method for robust characterization of estrogen-regulated microRNA profiles. The activity of estrogen is mediated by two nuclear receptors, estrogen receptor alpha and estrogen receptor beta, and a transmembrane G-protein coupled estrogen recept作者: Audiometry 時(shí)間: 2025-3-24 20:31
Molecular Cloning and Purification of the Protein Lysine Methyltransferase SMYD2 and its Co-crystalhis protocol describes SMYD2 molecular cloning and purification and crystallization of SMYD2 in complex with an ERα peptide. Recombinant SMYD2 is constructed and overexpressed in . cells. After release from the cells by French Press, SMYD2 is purified to apparent homogeneity with multiple chromatogr作者: 勤勉 時(shí)間: 2025-3-25 03:10 作者: DUCE 時(shí)間: 2025-3-25 03:40
Nietzsche, Anti-Semitism and Mass Murder,PB, the conserved motif within the DBD activates the . gene. When this motif was mutated, the activation of . was suppressed significantly. This chapter also describes the use of a phospho-peptide antibody (αP-S216) in the chromatin immunoprecipitation (ChIP) assay, and the co-immunoprecipitation (Co-IP) assay visualized by Western blot analysis.作者: glucagon 時(shí)間: 2025-3-25 11:12
Communication, Culture and Change in Asiassion of angioregulatory factors, e.g., endothelial nitric oxide synthase (eNOS) in the vasculature, vascular endothelial growth factor (VEGF) in the placenta, and melanocortin 2 receptor (MC2R)/accessory protein (MRAP) in the fetal adrenal of the nonhuman primate.作者: 最有利 時(shí)間: 2025-3-25 14:35
Antibody Validation for Estrogen Receptor Beta, well-characterized positive and negative controls. The validation scheme presented is applicable for immunohistochemistry and Western blotting. The protocol includes evaluation of mRNA evidence, use of public databases, assessment of on- and off-target binding, and an optional step for corroboration with immunoprecipitation and mass spectrometry.作者: humectant 時(shí)間: 2025-3-25 19:29
,Immunoprecipitation Analyses of Estrogen Receptor α Phosphorylated at Serine 216 in the Mouse LiverPB, the conserved motif within the DBD activates the . gene. When this motif was mutated, the activation of . was suppressed significantly. This chapter also describes the use of a phospho-peptide antibody (αP-S216) in the chromatin immunoprecipitation (ChIP) assay, and the co-immunoprecipitation (Co-IP) assay visualized by Western blot analysis.作者: eucalyptus 時(shí)間: 2025-3-25 20:17 作者: 經(jīng)典 時(shí)間: 2025-3-26 00:26 作者: AUGUR 時(shí)間: 2025-3-26 05:12
Cultural Analysis and Climate Resilience,s (shRNAs) specifically designed to silence ERα, the construction of the adeno-associated viral (AAV) vector for delivery of the shRNA, the procedures to confirm the silencing of ERα (in vitro and in vivo) and in vivo delivery of the shRNAs to the brains of animals.作者: infinite 時(shí)間: 2025-3-26 10:18
the estrogenic activity (i.e., both receptor-mediated agonism and/or antagonism) of these compounds. This chapter focuses on methods that allow determination of whether an ERα-binding compound acts as an agonist or antagonist of the receptor and whether the compound induces degradation of the receptor.作者: IST 時(shí)間: 2025-3-26 15:39
https://doi.org/10.1007/978-1-349-07867-7, however, have been more difficult, in part due to a lack of endogenous expression in cell lines and lack of specific antibodies. In this chapter, we provide an optimized stepwise ChIP protocol for a well-validated ERβ antibody, which is applicable for ChIP-Seq analysis of cell lines with exogenous expression of ERβ.作者: vocation 時(shí)間: 2025-3-26 18:55
,Immunofluorescent Verification of Silencing Estrogen Receptor α with siRNA in the Intact Rodent Bras (shRNAs) specifically designed to silence ERα, the construction of the adeno-associated viral (AAV) vector for delivery of the shRNA, the procedures to confirm the silencing of ERα (in vitro and in vivo) and in vivo delivery of the shRNAs to the brains of animals.作者: expunge 時(shí)間: 2025-3-26 22:15
Assessing Estrogenic Activity of Classical Estrogen Receptor -Binding Compounds, the estrogenic activity (i.e., both receptor-mediated agonism and/or antagonism) of these compounds. This chapter focuses on methods that allow determination of whether an ERα-binding compound acts as an agonist or antagonist of the receptor and whether the compound induces degradation of the receptor.作者: badinage 時(shí)間: 2025-3-27 03:42
An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of Estrogen Receptor Beta,, however, have been more difficult, in part due to a lack of endogenous expression in cell lines and lack of specific antibodies. In this chapter, we provide an optimized stepwise ChIP protocol for a well-validated ERβ antibody, which is applicable for ChIP-Seq analysis of cell lines with exogenous expression of ERβ.作者: 親屬 時(shí)間: 2025-3-27 09:05
Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells,or 1. This chapter details how to prepare cells for optimal estrogen response, directions for estrogen treatment, RNA extraction, different microRNA profiling approaches, and subsequent confirmations.作者: Carcinogenesis 時(shí)間: 2025-3-27 09:34
,Immunoblot Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 /GPERd subsequent immunoblotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estrogen receptor (mER), GPR30 or GPER1. Hence, phosphorylation represents a crosstalk event between GPR30 and ERα and may be important in estrogen-regulated physiology.作者: 虛弱的神經(jīng) 時(shí)間: 2025-3-27 15:19
Extraction of RNA and Analysis of Estrogen-Responsive Genes by RT-qPCR,bust and allows the measurement of gene expression in a variety of tissues and cells. This chapter describes the protocols used for RNA extraction and analysis as well as for RT-qPCR assay using hydrolysis (TaqMan-type) probes.作者: Hemodialysis 時(shí)間: 2025-3-27 18:27 作者: Expostulate 時(shí)間: 2025-3-28 01:27 作者: 圍裙 時(shí)間: 2025-3-28 05:53
1064-3745 expertsThis fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ) as well as the non-genomic membrane-boun作者: PAN 時(shí)間: 2025-3-28 10:08
https://doi.org/10.1007/978-3-031-44294-0d subsequent immunoblotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estrogen receptor (mER), GPR30 or GPER1. Hence, phosphorylation represents a crosstalk event between GPR30 and ERα and may be important in estrogen-regulated physiology.作者: 優(yōu)雅 時(shí)間: 2025-3-28 14:18
The Italian Communist Party and Culturebust and allows the measurement of gene expression in a variety of tissues and cells. This chapter describes the protocols used for RNA extraction and analysis as well as for RT-qPCR assay using hydrolysis (TaqMan-type) probes.作者: VEIL 時(shí)間: 2025-3-28 16:48
https://doi.org/10.1007/978-3-031-44995-6knowledge of estrogen receptor specificity of different ligands as well as dynamics of estrogen signaling. Coupled to image analysis, the model can provide quantitative concentration-response information on estrogenic activity of chemical compounds.作者: Obliterate 時(shí)間: 2025-3-28 22:41 作者: 未開化 時(shí)間: 2025-3-29 00:50
https://doi.org/10.1007/978-3-031-44294-0lational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immunoblotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estro作者: DEAWL 時(shí)間: 2025-3-29 04:44 作者: Euthyroid 時(shí)間: 2025-3-29 07:19 作者: Exclaim 時(shí)間: 2025-3-29 11:31 作者: 外觀 時(shí)間: 2025-3-29 17:24 作者: Feature 時(shí)間: 2025-3-29 20:21
Afia Akhter Lipi,Yukiko Nakano,Matthias Rehmssion to a distinct cell population. Therefore, ISH may be used in basic cell biology to detect the expression of certain genes within a tissue containing various cell populations. Here, we describe the detection and cellular localization of two estrogen receptors, both isoforms of the genomic estro作者: goodwill 時(shí)間: 2025-3-30 01:08 作者: 異常 時(shí)間: 2025-3-30 08:05 作者: cortex 時(shí)間: 2025-3-30 10:36 作者: 不能和解 時(shí)間: 2025-3-30 13:23
https://doi.org/10.1007/978-3-031-44995-6n reporter zebrafish as an in vivo model for the identification of estrogenic properties of compounds. Live imaging of these transgenic fish provides knowledge of estrogen receptor specificity of different ligands as well as dynamics of estrogen signaling. Coupled to image analysis, the model can pr作者: hyperuricemia 時(shí)間: 2025-3-30 20:02
articular as antagonists for the treatment of ERα-positive breast cancers. However, ERα-targeted agonists have also been clinically useful (e.g., for the treatment of osteoporosis). The breast cancer field is regularly identifying novel ERα-binding compounds with the goal of identifying new potentia作者: 不真 時(shí)間: 2025-3-30 21:49 作者: incarcerate 時(shí)間: 2025-3-31 01:20 作者: 大方不好 時(shí)間: 2025-3-31 07:11
https://doi.org/10.1057/9780230106437n responses to intra- and extracellular signals. Moreover, dysregulated mRNA translation is a common feature in disease states, including neurological disorders and cancer. Yet, most studies of gene expression focus on analysis of mRNA levels, leaving variations in translational efficiencies largely作者: 組裝 時(shí)間: 2025-3-31 11:52
The Politics of Culture and Community,–31, 2015). Tools to measure genetic variation have matured significantly throughout this advancement in knowledge (Lenoir and Giannella. J Biomed Discov Collab 1:11, 2006; Marzancola et al. Methods Mol Biol 1368:161–178, 2016). In this chapter, the focus is on the laboratory methods developed to pe作者: 有限 時(shí)間: 2025-3-31 16:50
Culture and Immigration in Contexts, tissues, biological fluids, secretome, etc.) is a useful strategy to identify proteins and analyze their changes. Samples processed through a gel-free approach provide a simple method for protein separation and profile comparison of different conditions, such as using fewer steps in the protocol,作者: FLORA 時(shí)間: 2025-3-31 18:51
The Culture and Institutions of Japan,nd can also function as biomarkers. Here, we describe a method for robust characterization of estrogen-regulated microRNA profiles. The activity of estrogen is mediated by two nuclear receptors, estrogen receptor alpha and estrogen receptor beta, and a transmembrane G-protein coupled estrogen recept作者: 宴會(huì) 時(shí)間: 2025-4-1 01:17 作者: 誘導(dǎo) 時(shí)間: 2025-4-1 05:22 作者: LEERY 時(shí)間: 2025-4-1 08:01 作者: Dysarthria 時(shí)間: 2025-4-1 12:35
Kathleen M. EysterIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 說不出 時(shí)間: 2025-4-1 15:50 作者: surrogate 時(shí)間: 2025-4-1 22:20 作者: alleviate 時(shí)間: 2025-4-2 02:31 作者: ATOPY 時(shí)間: 2025-4-2 02:56
