標題: Titlebook: Estrogen Receptors; Methods and Protocol Kathleen M. Eyster Book 2016 Springer Science+Business Media New York 2016 RNAseq.estrogen recepto [打印本頁] 作者: papyrus 時間: 2025-3-21 18:01
書目名稱Estrogen Receptors影響因子(影響力)
書目名稱Estrogen Receptors影響因子(影響力)學科排名
書目名稱Estrogen Receptors網(wǎng)絡公開度
書目名稱Estrogen Receptors網(wǎng)絡公開度學科排名
書目名稱Estrogen Receptors被引頻次
書目名稱Estrogen Receptors被引頻次學科排名
書目名稱Estrogen Receptors年度引用
書目名稱Estrogen Receptors年度引用學科排名
書目名稱Estrogen Receptors讀者反饋
書目名稱Estrogen Receptors讀者反饋學科排名
作者: Neuralgia 時間: 2025-3-21 23:49
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/315807.jpg作者: Eeg332 時間: 2025-3-22 03:02
978-1-4939-5006-5Springer Science+Business Media New York 2016作者: 誹謗 時間: 2025-3-22 05:11
Estrogen Receptors978-1-4939-3127-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 預定 時間: 2025-3-22 10:42
https://doi.org/10.1007/978-1-349-21559-1NGS) to determine DNA-binding sites of a protein of interest on a genome-wide level, importantly, allowing for de novo discovery of binding events. Here we describe ChIP-seq using the well-established example of estrogen receptor-α mapping in the MCF7 breast cancer cell line.作者: 哀求 時間: 2025-3-22 16:54
gy can be used for the identification of differential gene expression, genetic mutations associated with diseases, DNA methylation, single-nucleotide polymorphisms, and microRNA expression, to name a few. This chapter describes microarray technology for the analysis of differential gene expression in response to estrogen treatment.作者: 哀求 時間: 2025-3-22 20:57 作者: Melodrama 時間: 2025-3-22 23:15 作者: 形狀 時間: 2025-3-23 02:46
https://doi.org/10.1007/978-1-4939-3127-9RNAseq; estrogen receptor; genomic nuclear estrogen receptors; nongenomic estrogen receptor; proximity l作者: Ovulation 時間: 2025-3-23 09:18 作者: musicologist 時間: 2025-3-23 12:47 作者: 違法事實 時間: 2025-3-23 17:03
Cultural and Social Justice Counseling transcription levels. The technique is especially useful for measuring estrogen receptor transcript levels as well as gene expression changes in response to estrogen stimulation as it is quick, accurate, robust, and allows the measurement of gene expression in a variety of tissues and cells. This c作者: Awning 時間: 2025-3-23 19:51
https://doi.org/10.1007/978-3-319-64039-6teroid hormone receptors . and .. These bind to DNA and modulate the expression of target genes. Identification of estrogen target genes is greatly facilitated by the use of transcriptomic methods, such as RNA-seq and expression microarrays, and chromatin immunoprecipitation with massively parallel 作者: 不在灌木叢中 時間: 2025-3-23 23:21 作者: 逃避系列單詞 時間: 2025-3-24 06:10 作者: palliative-care 時間: 2025-3-24 09:21
Peer S. Daugbjerg,Martin Krabbe Sillasene cell. There are two previously identified two canonical estrogen response elements (ERE1 and ERE2) present in the 5′-flanking region of the . gene which is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the . promoter i作者: jarring 時間: 2025-3-24 10:46
https://doi.org/10.1007/978-1-349-21559-1NGS) to determine DNA-binding sites of a protein of interest on a genome-wide level, importantly, allowing for de novo discovery of binding events. Here we describe ChIP-seq using the well-established example of estrogen receptor-α mapping in the MCF7 breast cancer cell line.作者: 乳汁 時間: 2025-3-24 17:10
Anne Edwards,Marilyn Fleer,Louise B?ttcherndance of isoforms and finding novel transcripts. In this chapter, we describe a protocol to construct an RNA-Seq library for sequencing on Illumina NGS platforms, and a computational pipeline to perform RNA-Seq data analysis. The protocols described in this chapter can be applied to the analysis of作者: Gudgeon 時間: 2025-3-24 21:57 作者: Arrhythmia 時間: 2025-3-24 23:49 作者: canvass 時間: 2025-3-25 03:54
https://doi.org/10.1057/978-1-137-53339-5teraction, and protein modifications in cells and tissues. The proximity ligation assay (PLA), a method of detection that combines immunologic and PCR-based approaches, was developed to overcome some of the drawbacks that are inherent to other detection methods. The PLA allows for very sensitive and作者: genesis 時間: 2025-3-25 11:22 作者: 聯(lián)邦 時間: 2025-3-25 14:48
Grundlagen und Gegenstand der Untersuchung,ate the receptor before and after estradiol stimulation. More often than not these experiments were performed using postmortem, lysed, or fixed tissue samples, whose tissue or cellular structure is typically severely altered or at times completely lost, making the definitive localization of estrogen作者: 消音器 時間: 2025-3-25 18:39 作者: Aggressive 時間: 2025-3-25 23:12
https://doi.org/10.1007/978-3-030-46126-3his protocol describes SMYD2 purification and crystallization of SMYD2 in complex with an ERα peptide. Recombinant SMYD2 is overexpressed in . cells. After release from the cells by French Press, SMYD2 is purified to apparent homogeneity with multiple chromatography methods. Nickel affinity column p作者: Dislocation 時間: 2025-3-26 02:32
G. D. Ehrlich,P. J. DeMeo,J. W. Costerton function. The interaction of ERs with DNA sequences, known as estrogen response elements (EREs) (a palindromic repeat separated by three-base spacer, 5′GGTCAnnnTGACC-3′), is required for estrogen regulation of target gene expression. Here, we describe a simple “mix-and-measure”-based method for det作者: maudtin 時間: 2025-3-26 06:35
Political Economy of New Silk Road Culture,nding of estrogen with the receptors shows changes in the resonance structure and movement of protons. We cloned ERβ and its trans-activation domain (TAD) and ligand-binding domain (LBD), expressed them in prokaryotic expression vectors, purified them, and studied their interaction with estradiol. I作者: DOLT 時間: 2025-3-26 09:03
https://doi.org/10.1007/978-3-031-53836-0his process occurs with the contribution of protein and peptide partners (also called coactivators) that can modulate the structure of ERα, and therefore its specificity of action. As with most transcription factors, ERα exhibits a high content of α helix, making it difficult to routinely run spectr作者: Gerontology 時間: 2025-3-26 12:46 作者: 停止償付 時間: 2025-3-26 19:34
https://doi.org/10.1007/978-3-319-46328-5 rat uterus after estrogen (ethinylestradiol) treatment. The steps of the protocol involve sample preparation (digestion), 2D-nanoLC-MS/MS analysis, and shotgun proteomics analysis including bioinformatics tools for data conversion, organization, and interpretation.作者: filicide 時間: 2025-3-26 22:51 作者: 輕彈 時間: 2025-3-27 04:43
Competitive Binding Assay for the G-Protein-Coupled Receptor 30 (GPR30) or G-Protein-Coupled Estroges are essential to elucidate the underlying effects of this novel estrogen metabolite and to validate its targets; therefore, this competitive receptor-binding assay protocol was developed in order to assess the membrane receptor binding and affinity of 2-methyoxyestradiol.作者: 不知疲倦 時間: 2025-3-27 06:09
Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two-Dimensional Liquid Chromato rat uterus after estrogen (ethinylestradiol) treatment. The steps of the protocol involve sample preparation (digestion), 2D-nanoLC-MS/MS analysis, and shotgun proteomics analysis including bioinformatics tools for data conversion, organization, and interpretation.作者: 毀壞 時間: 2025-3-27 12:46
Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction Between the Estroly structured in α-helix, is a key coactivator for ERα activity. Here, we show how circular dichroism can be used to study the interaction of ERα with Ca.-calmodulin. Our approach allows the determination not only of the conformational changes induced upon complex formation but also the dissociation constant (.) of this interaction.作者: AGONY 時間: 2025-3-27 14:03
Book 2016n this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen rece作者: abolish 時間: 2025-3-27 18:10 作者: Sad570 時間: 2025-3-27 22:40 作者: CIS 時間: 2025-3-28 02:27 作者: 神圣在玷污 時間: 2025-3-28 06:56
Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of , in TM4 Sertoli Cells,hich is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the . promoter in TM4 mouse Sertoli cells. This chapter describes the protocol for chromatin immunoprecipitation applied to the estrogen response elements in the . promoter.作者: 豎琴 時間: 2025-3-28 10:52 作者: Systemic 時間: 2025-3-28 17:22
,In Situ Hybridization of Estrogen Receptors α and β and GPER in the Human Testis,ning various cell populations. Here, we describe the detection and cellular localization of three estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) as well as the membrane-bound G-protein-coupled estrogen receptor 1 (GPER) in the human testis.作者: 偉大 時間: 2025-3-28 21:40 作者: Lime石灰 時間: 2025-3-29 01:39
Political Economy of New Silk Road Culture,n this chapter, a detailed method of preparation of recombinant proteins, SDS-PAGE, silver staining, and NMR are described. Such methods are useful to check the biological activity of bacterially expressed proteins and are applicable to basic and applied research.作者: ITCH 時間: 2025-3-29 05:27 作者: 流動才波動 時間: 2025-3-29 08:25
https://doi.org/10.1007/978-94-007-5107-1ary gland. The purpose of this discussion of the estrogen receptors is to provide a brief overview of the estrogen receptors and estrogen action from perspectives such as the historical, physiological, pharmacological, pathological, structural, and ligand perspectives.作者: conception 時間: 2025-3-29 13:27 作者: 山間窄路 時間: 2025-3-29 18:22 作者: CHOP 時間: 2025-3-29 21:14
Peer S. Daugbjerg,Martin Krabbe Sillasenhich is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the . promoter in TM4 mouse Sertoli cells. This chapter describes the protocol for chromatin immunoprecipitation applied to the estrogen response elements in the . promoter.作者: IDEAS 時間: 2025-3-30 01:45
Anne Edwards,Marilyn Fleer,Louise B?ttcherGS platforms, and a computational pipeline to perform RNA-Seq data analysis. The protocols described in this chapter can be applied to the analysis of differential gene expression in control versus 17β-estradiol treatment of in vivo or in vitro systems.作者: 撤退 時間: 2025-3-30 06:49
Culture Hacks strategisch einsetzenning various cell populations. Here, we describe the detection and cellular localization of three estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) as well as the membrane-bound G-protein-coupled estrogen receptor 1 (GPER) in the human testis.作者: ATRIA 時間: 2025-3-30 08:24
Bioinformatics Analysis of Estrogen-Responsive Genes,sequencing (ChIP-seq). Combining transcriptomic and ChIP-seq data enables a distinction to be drawn between direct and indirect estrogen target genes. This chapter discusses some methods of identifying estrogen target genes that do not require any expertise in programming languages or complex bioinformatics.作者: 信條 時間: 2025-3-30 15:51 作者: 膠狀 時間: 2025-3-30 16:53
https://doi.org/10.1057/978-1-137-53339-5y factors, e.g., angiopoietin-1 (Ang-1) in the endometrium, vascular endothelial growth factor (VEGF) in the placenta, and melanocortin 2 receptor (MC2R)/accessory protein (MRAP) in the fetal adrenal of the nonhuman primate.作者: 商議 時間: 2025-3-30 22:27 作者: mitral-valve 時間: 2025-3-31 01:12
G. D. Ehrlich,P. J. DeMeo,J. W. Costertonalso accurately detect all 15 singly mutated EREs (i.e., three possible base substitutions at each of one to five positions from left to right of the 5′ end half site, GGTCA) for their binding energy to ER. This method is compatible with 96-well plate format for high-throughput analysis.作者: 外形 時間: 2025-3-31 06:57
Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Py factors, e.g., angiopoietin-1 (Ang-1) in the endometrium, vascular endothelial growth factor (VEGF) in the placenta, and melanocortin 2 receptor (MC2R)/accessory protein (MRAP) in the fetal adrenal of the nonhuman primate.作者: 單色 時間: 2025-3-31 10:30
Colocalization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by ptors, this novel fluorescent probe is also suitable for the identification of unconventional targets such membrane estrogen receptors as well as other noncanonical targets, some of which are likely responsible for the number of undesired side effects reported during long-term tamoxifen treatments.作者: fluffy 時間: 2025-3-31 13:53 作者: 靈敏 時間: 2025-3-31 18:19
1064-3745 etailed and helpful resource for scientists who are intrigued by the many facets of estrogen..The chapter ‘Bioinformatics Analysisof Estrogen-Responsive Genes‘ is open access under a CC BY 4.0 license.?.978-1-4939-5006-5978-1-4939-3127-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Abjure 時間: 2025-4-1 01:26
Book 2016wn pitfalls..Cutting-edge and thorough, .Estrogen Receptors: Methods and Protocols .is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen..The chapter ‘Bioinformatics Analysisof Estrogen-Responsive Genes‘ is open access under a CC BY 4.0 license.?.作者: Decimate 時間: 2025-4-1 03:15 作者: EXUDE 時間: 2025-4-1 08:02 作者: 牛馬之尿 時間: 2025-4-1 12:26 作者: INTER 時間: 2025-4-1 17:50
Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors,omoters of estrogen-dependent genes is a common mechanism to activate or enhance gene transcription in breast cancer thus promoting tumor progression. In this chapter, we demonstrate a stepwise protocol for ChIP assay using binding of ERα to its genomic targets after stimulation with 17β-estradiol (
