標(biāo)題: Titlebook: Erythropoiesis; Methods and Protocol Joyce A. Lloyd Book 2018 Springer Science+Business Media LLC 2018 Animal models.Red blood cells.Progen [打印本頁] 作者: vein220 時(shí)間: 2025-3-21 17:21
書目名稱Erythropoiesis影響因子(影響力)
書目名稱Erythropoiesis影響因子(影響力)學(xué)科排名
書目名稱Erythropoiesis網(wǎng)絡(luò)公開度
書目名稱Erythropoiesis網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Erythropoiesis被引頻次
書目名稱Erythropoiesis被引頻次學(xué)科排名
書目名稱Erythropoiesis年度引用
書目名稱Erythropoiesis年度引用學(xué)科排名
書目名稱Erythropoiesis讀者反饋
書目名稱Erythropoiesis讀者反饋學(xué)科排名
作者: macular-edema 時(shí)間: 2025-3-21 20:58 作者: FECK 時(shí)間: 2025-3-22 02:57 作者: Mutter 時(shí)間: 2025-3-22 05:52 作者: INTER 時(shí)間: 2025-3-22 10:49
Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands,and maintain the integrity of the niche. To improve our understanding of the molecular regulation of erythroid cells in EBIs, we have developed protocols for immuno-gold labeling of erythroid surface antigens to combine with scanning electron microscopy. These protocols have allowed imaging of EBIs 作者: Metamorphosis 時(shí)間: 2025-3-22 15:46
Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells,t is possible to unambiguously distinguish erythroblasts at each developmental stage during murine terminal erythroid differentiation. We also developed methods for the analysis and isolation of human erythroid cells at all developmental stages. BFU-E and CFU-E are characterized by CD45.GPA.IL-3R.CD作者: Metamorphosis 時(shí)間: 2025-3-22 17:51 作者: Morsel 時(shí)間: 2025-3-22 21:45
High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure,unofluorescence staining and confocal imaging of various molecules, organelles and structures of interest in erythroblasts, and to present and quantitatively analyze the data obtained in these fluorescence images.作者: PTCA635 時(shí)間: 2025-3-23 02:49
Book 2018Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Erythropoie作者: 上腭 時(shí)間: 2025-3-23 05:35
,China’s Rural Economic Restructuring,ery useful cell lines have been developed by manipulating ES or fetal liver erythroid progenitor cells from knockout mouse models. In recent years, our understanding of erythropoiesis has improved, due to the ability to knock down genes in native human hematopoietic stem and progenitor cells derived作者: 取回 時(shí)間: 2025-3-23 12:42 作者: Relinquish 時(shí)間: 2025-3-23 17:26 作者: Arrhythmia 時(shí)間: 2025-3-23 21:01 作者: Detoxification 時(shí)間: 2025-3-24 01:47 作者: 努力趕上 時(shí)間: 2025-3-24 04:43
Chung-Hua Shen,Qi Liang,Xiang-Chao Haot is possible to unambiguously distinguish erythroblasts at each developmental stage during murine terminal erythroid differentiation. We also developed methods for the analysis and isolation of human erythroid cells at all developmental stages. BFU-E and CFU-E are characterized by CD45.GPA.IL-3R.CD作者: 恃強(qiáng)凌弱的人 時(shí)間: 2025-3-24 09:27
https://doi.org/10.1007/978-3-642-25887-9f large numbers of cells, which can be quantitated for both morphometric and fluorescent characteristics. Over the past 10?years, this approach has been increasingly used to study aspects of erythropoiesis. This chapter describes how to utilize IFC to enumerate multiple specific stages of erythropoi作者: 激怒某人 時(shí)間: 2025-3-24 11:11 作者: 悶熱 時(shí)間: 2025-3-24 18:25
1064-3745 opics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Erythropoie978-1-4939-8483-1978-1-4939-7428-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 間接 時(shí)間: 2025-3-24 20:44 作者: Entropion 時(shí)間: 2025-3-25 00:38
spectively. Another nuclei acid staining dye, SYTO16, is used for the assessment of human enucleation in combination with FSC. For human cells, the three populations that represent nucleated erythroblasts, reticulocyte, and extruded nuclei are identified as FSC. SYTO16., FSC. SYTO16., FSC.SYTO16., respectively.作者: patella 時(shí)間: 2025-3-25 04:11 作者: HALO 時(shí)間: 2025-3-25 10:14
Stress Erythropoiesis Model Systems,s despite having no effect on steady-state erythropoiesis. In this chapter, we will discuss in vivo and in vitro techniques to study stress erythropoiesis in mice and how the in vitro culture system can be extended to study human stress erythropoiesis.作者: 喃喃訴苦 時(shí)間: 2025-3-25 13:02
Flow Cytometric Analysis of Erythroblast Enucleation,spectively. Another nuclei acid staining dye, SYTO16, is used for the assessment of human enucleation in combination with FSC. For human cells, the three populations that represent nucleated erythroblasts, reticulocyte, and extruded nuclei are identified as FSC. SYTO16., FSC. SYTO16., FSC.SYTO16., respectively.作者: 大包裹 時(shí)間: 2025-3-25 18:38
Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Maingle dose (approx. 1–2?×?10.). In this chapter, we will review critical areas in the development and good manufacturing practice (GMP) translation of cellular therapeutics through to early phase clinical trials and how this learning can be applied to in vitro RBC therapies.作者: 割公牛膨脹 時(shí)間: 2025-3-26 00:02 作者: 失誤 時(shí)間: 2025-3-26 01:43 作者: GOAD 時(shí)間: 2025-3-26 08:14
1064-3745 Contains key notes and implementation advice from the expertThis detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include 作者: 手工藝品 時(shí)間: 2025-3-26 09:13 作者: JEER 時(shí)間: 2025-3-26 16:39
closely resemble adult erythroid cells. This chapter describes culture protocols for the maintenance and erythroid differentiation of HUDEP-2 cells. Methods to genetically manipulate HUDEP-2 cells using a CRISPR/Cas9 nuclease-based approach are also discussed.作者: habitat 時(shí)間: 2025-3-26 18:51
Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models,l disease. Analysis of erythroid parameters provides insights into the RBC physiologic or pathophysiologic status. Mice expressing both murine and human globin genes can be investigated using adapted protocols provided herein.作者: Clinch 時(shí)間: 2025-3-26 21:38 作者: 宇宙你 時(shí)間: 2025-3-27 04:35
nces and stringent functional tests must also be applied. Here, we present methodology suitable for 3C experiments that can also be applied as the basis for related 4C, 5C, and Hi-C approaches. These procedures are widely applicable to erythroid cell lines, progenitor cells, and tissues.作者: GEM 時(shí)間: 2025-3-27 05:41 作者: liaison 時(shí)間: 2025-3-27 11:07 作者: 釘牢 時(shí)間: 2025-3-27 14:35 作者: DEBT 時(shí)間: 2025-3-27 20:46
,Expansion of China’s Textile Exports,ss small hairpin RNA (shRNA) is an efficient method to downregulate genes. Flow cytometric analyses are used to enrich for erythroid cells. Using these methods, one can generate in vitro differentiated cells to use for quantitative reverse transcriptase PCR and other purposes.作者: 簡(jiǎn)潔 時(shí)間: 2025-3-28 01:22
Mouse Models of Erythropoiesis and Associated Diseases,uts, and genome editing, provide a significant resource to the research community to test a plethora of hypotheses. This review summarizes the mouse models available for studying a wide variety of erythroid-related questions, as well as the properties inherent in each one.作者: PRO 時(shí)間: 2025-3-28 05:27
In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Corss small hairpin RNA (shRNA) is an efficient method to downregulate genes. Flow cytometric analyses are used to enrich for erythroid cells. Using these methods, one can generate in vitro differentiated cells to use for quantitative reverse transcriptase PCR and other purposes.作者: 成績上升 時(shí)間: 2025-3-28 07:57 作者: 招人嫉妒 時(shí)間: 2025-3-28 11:10 作者: Anthem 時(shí)間: 2025-3-28 14:46
978-1-4939-8483-1Springer Science+Business Media LLC 2018作者: Accede 時(shí)間: 2025-3-28 18:54
Erythropoiesis978-1-4939-7428-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 水獺 時(shí)間: 2025-3-29 02:33
,China’s Rural Economic Restructuring, was examined in the mouse, chicken, sheep, goat, and rabbit, among other vertebrates. Erythroid cell lines derived from human blood cancers were also very useful, as they could be genetically manipulated more easily than whole animals. Genetic models in the mouse, zebrafish, and frog have provided 作者: intercede 時(shí)間: 2025-3-29 03:17 作者: 昏迷狀態(tài) 時(shí)間: 2025-3-29 08:56 作者: transplantation 時(shí)間: 2025-3-29 13:42 作者: 頭盔 時(shí)間: 2025-3-29 15:50
https://doi.org/10.1007/978-981-15-9227-0 the senescent erythrocytes that are removed by macrophages in the spleen. In contrast, anemic or hypoxic stress induces a physiological response designed to increase oxygen delivery to the tissues. Stress erythropoiesis is a key component of this response. It is best understood in mice where it is 作者: 晚來的提名 時(shí)間: 2025-3-29 23:18
https://doi.org/10.1007/978-3-030-53959-7us approaches to evaluate the global red blood cell properties in mouse models of human hemoglobin disorders, in particular thalassemia and sickle cell disease. Analysis of erythroid parameters provides insights into the RBC physiologic or pathophysiologic status. Mice expressing both murine and hum作者: 現(xiàn)暈光 時(shí)間: 2025-3-30 01:45
Financing China’s Electricity Sectore progenitors and has greatly contributed to our understanding of erythropoiesis. Studies since the 1970s have led to the development of a model of the erythron, whereby the earliest erythroid-committed progenitor, the immature burst-forming unit erythroid (BFU-E), gives rise sequentially to late-st作者: antiandrogen 時(shí)間: 2025-3-30 08:07 作者: Glossy 時(shí)間: 2025-3-30 12:17
Chung-Hua Shen,Qi Liang,Xiang-Chao Haot. During the past decade, considerable progress has been made on the development of flow cytometry (FCM) and fluorescence-activated cell sorting (FACS) methods for the analysis and isolation of both murine and human erythroid cells at distinct stages of erythropoiesis, based on changes in the expre作者: 說笑 時(shí)間: 2025-3-30 16:24 作者: Pcos971 時(shí)間: 2025-3-30 18:52
reticulocyte. Herein, we describe the flow cytometry-based assays for enucleation assessment. The separation of nucleated erythroblasts, reticulocytes, and extruded nuclei by flow cytometry is based on DNA staining, surface expression of erythrocyte specific markers, or forward scatter (FSC). The en作者: 樹上結(jié)蜜糖 時(shí)間: 2025-3-30 21:45 作者: 不舒服 時(shí)間: 2025-3-31 04:16
Jerry McBeath,Jenifer Huang McBeathNA and protein with bi-functional reagents such as formaldehyde and precipitation of the protein with a specific antibody permit PCR amplification (ChIP) or sequencing (ChIP-seq) to identify the bound sites. Here, we present methodology for these approaches that are widely applicable to erythroid ce
