標(biāo)題: Titlebook: Epstein-Barr Virus Protocols; Joanna B. Wilson,Gerhard H. W. May Book 2001 Humana Press 2001 [打印本頁] 作者: 帳簿 時(shí)間: 2025-3-21 19:48
書目名稱Epstein-Barr Virus Protocols影響因子(影響力)
作者: 相反放置 時(shí)間: 2025-3-21 23:10 作者: Myofibrils 時(shí)間: 2025-3-22 03:13
Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell LeveNA migrate with known and different mobilities, the Gardella gel (.), and combined it with a highly sensitive DNA polymerase chain reaction (PCR) system (.,., and . in this volume), that can be used to detect a single copy of the viral genome in 10. uninfected cells. The result is a technique that c作者: 過分 時(shí)間: 2025-3-22 07:38
Virus Isolationne (.) and the Akata cell line (.). B95-8 cells and P3HR1 cells are usually induced with phorbol esters, sodium butyrate, or a mixture of the two (.). In addition, replication can be induced in the Akata cell line by crosslinking cell surface immunoglobulin (.).作者: Brittle 時(shí)間: 2025-3-22 12:27 作者: 眉毛 時(shí)間: 2025-3-22 14:40
Transient Gene Expression and MACS Enrichmentociated with a selection process (.,.). This selection process prevents the analysis of early effects of expressed viral genes and could also lead to secondary cell culture effects not associated with the viral gene. Often, inducible gene expression systems are difficult to modulate and can be leaky作者: 眉毛 時(shí)間: 2025-3-22 19:33
1064-3745 enic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late978-1-61737-137-0978-1-59259-227-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: prodrome 時(shí)間: 2025-3-22 21:38
Book 2001arch, namely, to gain sufficient insight into the viral–host interaction to be able to intercept the pathogenic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late作者: 馬賽克 時(shí)間: 2025-3-23 02:01 作者: heartburn 時(shí)間: 2025-3-23 08:00 作者: 懦夫 時(shí)間: 2025-3-23 11:02
https://doi.org/10.1007/978-1-4757-3500-0NA migrate with known and different mobilities, the Gardella gel (.), and combined it with a highly sensitive DNA polymerase chain reaction (PCR) system (.,., and . in this volume), that can be used to detect a single copy of the viral genome in 10. uninfected cells. The result is a technique that c作者: Bereavement 時(shí)間: 2025-3-23 15:54 作者: tangle 時(shí)間: 2025-3-23 20:04 作者: 無政府主義者 時(shí)間: 2025-3-24 00:51
Sociological Aspects of European Integrationociated with a selection process (.,.). This selection process prevents the analysis of early effects of expressed viral genes and could also lead to secondary cell culture effects not associated with the viral gene. Often, inducible gene expression systems are difficult to modulate and can be leaky作者: 有惡臭 時(shí)間: 2025-3-24 05:00 作者: Innocence 時(shí)間: 2025-3-24 07:39
Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCRated DNAs in both short-term and long-term assays (.). Quantitative, competitive PCR can be used to study both the contribution of proteins to the replication of oriP-based plasmids (.) as well as the requirements for specific DNA sequences to support replication of a plasmid (.). Advantages of this作者: GEAR 時(shí)間: 2025-3-24 10:53 作者: Rebate 時(shí)間: 2025-3-24 17:45
Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcriptsm a common promoter that exhibit a complex splicing pattern and contain a number of small open reading frames with the potential to encode a number of proteins (.). The CSTs have been detected in most types of EBV infection, including nasopharyngeal carcinoma (NPC) (.), oral hairy leukoplakia (.), E作者: reserve 時(shí)間: 2025-3-24 19:44
Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Ceir functions in relation to the physiology of the virus remain enigmatic. In recent years, the main interest in the EBERs has been in connection with the use of these RNAs as targets for identification of EBV infection using . hybridization. However evidence for a possible function for the EBERs is 作者: 完成 時(shí)間: 2025-3-25 01:05 作者: Coma704 時(shí)間: 2025-3-25 03:27
Detection of Epstein-Barr Virus DNA and Viral Gene Productsers of EBV-carrying B cells have been identified in the peripheral blood as well as in lymphoid and nonlymphoid tissues of chronic virus carriers (.,.). This is relevant to the study of human tumors. The detection of EBV DNA in a tumor by polymerase chain reaction (PCR) usually does not permit concl作者: Generic-Drug 時(shí)間: 2025-3-25 09:42 作者: Anonymous 時(shí)間: 2025-3-25 12:44 作者: spinal-stenosis 時(shí)間: 2025-3-25 18:05 作者: 止痛藥 時(shí)間: 2025-3-25 22:10
Virus Isolationer spontaneously move from latency into a lytic cycle of replication in a very small percentage of the population of most B-cell lines in culture and this number can, with varying degrees of efficiency, be increased by use of inducing agents. Typically, current production methods involve induction o作者: ALIBI 時(shí)間: 2025-3-26 00:32 作者: 入伍儀式 時(shí)間: 2025-3-26 05:32 作者: 偽造者 時(shí)間: 2025-3-26 11:56
Introduction of Plasmid Vectors into Cells Via Electroporation viral protein on the host cell separately from the effects of other viral proteins that are being concurrently expressed during a normal infection. Electroporation is a method of introducing a plasmid expressing the viral protein of interest into cells and, for long-term studies, generating cell li作者: oracle 時(shí)間: 2025-3-26 14:55
Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Geneinoma). Among about 90 genes encoded by the virus (.), seven latent genes—(Epstein-Barr viral nuclear antigen 1 (EBNA1), EBNA2, EBNA3A, EBNA3C, latent membrane protein 1 (LMP1), and EBERs—were found to be indispensable for B cell immortalization (.). LMP1 induces malignant transformation in rodent c作者: Priapism 時(shí)間: 2025-3-26 18:53
Transient Gene Expression and MACS Enrichment the efficiency of the transfection method. Lymphoid cells are effectively transfected by electroporation (.–.). However, the transfection efficiency varies usually between 5 and 20% of the living cells, depending on the cell line used. In the case of primary lymphoid cells, the transfection efficie作者: 顯微鏡 時(shí)間: 2025-3-26 23:37 作者: myelography 時(shí)間: 2025-3-27 02:27 作者: Hangar 時(shí)間: 2025-3-27 07:43 作者: Efflorescent 時(shí)間: 2025-3-27 09:33
Annals of Theoretical Psychologym a common promoter that exhibit a complex splicing pattern and contain a number of small open reading frames with the potential to encode a number of proteins (.). The CSTs have been detected in most types of EBV infection, including nasopharyngeal carcinoma (NPC) (.), oral hairy leukoplakia (.), E作者: antipsychotic 時(shí)間: 2025-3-27 16:08 作者: hematuria 時(shí)間: 2025-3-27 20:57 作者: 使更活躍 時(shí)間: 2025-3-28 01:00
Annegamento Soccorso tecnico e sanitarioers of EBV-carrying B cells have been identified in the peripheral blood as well as in lymphoid and nonlymphoid tissues of chronic virus carriers (.,.). This is relevant to the study of human tumors. The detection of EBV DNA in a tumor by polymerase chain reaction (PCR) usually does not permit concl作者: Antioxidant 時(shí)間: 2025-3-28 04:54 作者: 領(lǐng)導(dǎo)權(quán) 時(shí)間: 2025-3-28 07:50 作者: CRUDE 時(shí)間: 2025-3-28 14:26 作者: 打算 時(shí)間: 2025-3-28 15:52 作者: 潰爛 時(shí)間: 2025-3-28 18:57 作者: 高談闊論 時(shí)間: 2025-3-29 01:49 作者: Congeal 時(shí)間: 2025-3-29 03:58
https://doi.org/10.1007/978-94-015-3889-3 viral protein on the host cell separately from the effects of other viral proteins that are being concurrently expressed during a normal infection. Electroporation is a method of introducing a plasmid expressing the viral protein of interest into cells and, for long-term studies, generating cell li作者: Obliterate 時(shí)間: 2025-3-29 08:44 作者: Rankle 時(shí)間: 2025-3-29 12:39
Sociological Aspects of European Integration the efficiency of the transfection method. Lymphoid cells are effectively transfected by electroporation (.–.). However, the transfection efficiency varies usually between 5 and 20% of the living cells, depending on the cell line used. In the case of primary lymphoid cells, the transfection efficie作者: Assignment 時(shí)間: 2025-3-29 17:10
Epstein-Barr Virus Protocols978-1-59259-227-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Canary 時(shí)間: 2025-3-29 20:53
Annuaire Européen / European Yearbookxtracellular signals, EBV infects resting B-lymphocytes and drives the infected cells into the cell-division cycle. Recently, there has been much interest in defining the molecular mechanisms by which EBV reprograms the cell cycle control machinery in B-lympho-cytes (.–.).作者: 殺死 時(shí)間: 2025-3-30 02:54 作者: Dappled 時(shí)間: 2025-3-30 07:26 作者: Corral 時(shí)間: 2025-3-30 11:59 作者: Estimable 時(shí)間: 2025-3-30 15:03
Book 2001tion of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor’s etiology (Nature194:232–234, 1962). Since then, molecular and cellular biological and computational technologies have progressed by leaps and bounds. The advent of recombinant DNA tech作者: 心神不寧 時(shí)間: 2025-3-30 16:35
Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids the EBV nuclear antigens EBNA2, EBNA3a, and EBNA3c, and the latent membrane protein 1 (LMP1) (.–.). Since EBNA1 maintains the status of the EBV genomes in the proliferating B cells, it might also be indispensable (.).作者: linguistics 時(shí)間: 2025-3-30 23:14 作者: 氣候 時(shí)間: 2025-3-31 01:34
Visualizing EBV Expression Patterns by FISH has also been applied to visualization of specific viral RNAs within the nuclei of cells latently infected with EBV (.,.). . two-color detection of EBV-specific nuclear RNA allows the study of different viral expression programs at the cellular level (.).作者: NICHE 時(shí)間: 2025-3-31 07:05
https://doi.org/10.1007/978-1-4757-9191-4 assay include an increased sensitivity and a decreased time required to analyze samples relative to DNA blots, the traditional assay used to study replication of oriP-containing plasmids in the presence of EBNA-1 (.–.).作者: Lyme-disease 時(shí)間: 2025-3-31 11:32
Annegamento Soccorso tecnico e sanitariousions as to whether this is due to the presence of the virus in the tumor cell population or to the presence of EBV-carrying “by-stander” B cells in the tissue. For a meaningful analysis of EBV infection, it is therefore necessary in many instances to establish the cellular location of the virus using morphology-based techniques.
