作者: 我要威脅 時(shí)間: 2025-3-21 22:56
Single-Molecule Analysis of RNA Dynamics in Living Cells Using Molecular Beacons,n length, function as key regulators of gene expression in cellular physiology and pathogenesis. Greater understanding of lincRNA activities, particularly in the context of subcellular localization and dynamic regulation at the single-molecule level, is expected to provide in-depth understanding of 作者: neutral-posture 時(shí)間: 2025-3-22 03:24
Visualization of , Long Noncoding RNA with a Fluorescent CRISPR/Cas9 System,chromosome in mammalian females. But the mechanism by which . localizes and spreads on the X chromosome and facilitates transcriptional silencing remains largely unknown. This limited understanding, at least in part, is due to the technical difficulties in the visualization and functional characteri作者: 不理會(huì) 時(shí)間: 2025-3-22 06:21 作者: analogous 時(shí)間: 2025-3-22 10:37 作者: AGGER 時(shí)間: 2025-3-22 15:28
,Direct Chemical Biotinylation of RNA 5′-Ends Using a Diazo Reagent,ng of in vitro transcribed RNA are scarce. Herein, we describe experimental details for direct labeling of the 5′-phosphate of RNA using a diazo biotin-reagent, as exemplified on a 110 nucleotide RNA obtained via in vitro transcription. The method exploits the fact that, under neutral buffer conditi作者: AGGER 時(shí)間: 2025-3-22 19:29 作者: 蛛絲 時(shí)間: 2025-3-22 21:12 作者: 招致 時(shí)間: 2025-3-23 05:25 作者: Deduct 時(shí)間: 2025-3-23 08:17
Genome-Wide Annotation of circRNAs and Their Alternative Back-Splicing/Splicing with CIRCexplorer Pwide across different species. Interestingly, one gene locus can generate multiple circRNAs through alternative back-splicing and/or alternative splicing, thus expanding our understanding on the diversity and complexity of transcriptomes. Precise annotation of circRNAs with their alternative back-sp作者: byline 時(shí)間: 2025-3-23 13:42
,Synthesis and Evaluation of Novel Neamine–Nucleoside Conjugates as Potential Antibiotic Targets forNeamine–nucleoside conjugates could bind to the groove of RNA while the nucleobase moiety would bind specifically to the sequence of the 16S rRNA A-site fragment. Thus the designed conjugate compound . was found to have the same dissociation constant as neamine for binding to 16S rRNA and the neamin作者: invert 時(shí)間: 2025-3-23 14:30 作者: 體貼 時(shí)間: 2025-3-23 18:04 作者: Terminal 時(shí)間: 2025-3-24 01:15
LncVar: Deciphering Genetic Variations Associated with Long Noncoding Genes,ctions of most lncRNAs remain to be explored. Previous studies have revealed that a large amount of disease-associated variations are located in the lncRNA gene regions. To evaluate the effects of genetic variations on lncRNAs, we constructed a database of genetic variations associated with long non作者: fodlder 時(shí)間: 2025-3-24 04:26
Guided Reconstruction of Full-Length Isoforms from Short Reads by CIDANE,logies (RNA-seq) provide an immediate measurement of local splicing events, the phasing of these events along full-length isoforms requires the computational inference of long-range dependencies from short-range data points. We introduce CIDANE, a tool for the assembly and quantification of full-len作者: POLYP 時(shí)間: 2025-3-24 06:40 作者: 夾死提手勢(shì) 時(shí)間: 2025-3-24 14:38 作者: Mercantile 時(shí)間: 2025-3-24 15:18
5-Methylcytosine Analysis by RNA-BisSeq,wn or novel m.C sites have been validated by using advanced high-throughput techniques combined with next-generation sequencing (NGS), especially RNA bisulfite sequencing (RNA-BisSeq). Here we introduce an optimized RNA-BisSeq method by using ACT random hexamers to prime the reverse transcription of作者: ordain 時(shí)間: 2025-3-24 20:09 作者: 能得到 時(shí)間: 2025-3-25 01:42 作者: maladorit 時(shí)間: 2025-3-25 03:56 作者: 2否定 時(shí)間: 2025-3-25 08:33 作者: 到婚嫁年齡 時(shí)間: 2025-3-25 13:02 作者: 使虛弱 時(shí)間: 2025-3-25 19:49
Book 2019 targeted and unbiased high-throughput analysis associated with post-transcriptional RNA modification. The chapters in this book also cover specific topics such as transcriptome-wide detection of 5-methylcytosine; HAMR; iRNA-2OM; genome-wide annotation of circRNAs; immune-northern blotting; and dete作者: BATE 時(shí)間: 2025-3-25 21:59
Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine, aspects need to be considered before starting such a project. Below we describe a detailed step-by-step protocol for planning and performing a transcriptome-wide bisulfite sequencing experiment and subsequent data analysis to determine methyl-cytosine in poly(A)RNA from cells and tissues.作者: 得意牛 時(shí)間: 2025-3-26 00:38 作者: 人充滿活力 時(shí)間: 2025-3-26 07:13
,Direct Chemical Biotinylation of RNA 5′-Ends Using a Diazo Reagent,n-reagent, as exemplified on a 110 nucleotide RNA obtained via in vitro transcription. The method exploits the fact that, under neutral buffer conditions (~pH 6.8), the 5′-phosphate carries the only mildly acidic proton in the RNA molecule, which allows for selective functionalization at that site using diazo reagents.作者: Subjugate 時(shí)間: 2025-3-26 09:03
Identification of Methylated Transcripts Using the TRIBE Approach,ion. Here, we describe a complementary technique of standard meRIP-seq/miCLIP-seq approaches to identify methylated RNA using a?low amount of?material. We believe this approach can be applied in vivo to identify methylated targets in specific tissues or subpopulations of cells.作者: GOAD 時(shí)間: 2025-3-26 16:23 作者: Foolproof 時(shí)間: 2025-3-26 20:16
Peter Kopietz,Lorenz Bartosch,Florian SchützPR/Cas9-based approach. This strategy is?relatively simple approach to track?. at different stages of cell differentiation, providing mechanistic insights into the initiation, maintenance, and establishment of X inactivation.作者: 獨(dú)行者 時(shí)間: 2025-3-26 22:02
Visualization of , Long Noncoding RNA with a Fluorescent CRISPR/Cas9 System,PR/Cas9-based approach. This strategy is?relatively simple approach to track?. at different stages of cell differentiation, providing mechanistic insights into the initiation, maintenance, and establishment of X inactivation.作者: implore 時(shí)間: 2025-3-27 03:54 作者: novelty 時(shí)間: 2025-3-27 06:08 作者: demote 時(shí)間: 2025-3-27 10:05
https://doi.org/10.1007/978-3-322-98482-1ofile m.A along the transcriptome. We present here a protocol for m.A crosslinking immunoprecipitation sequencing (m.A-CLIP-seq), which profiles m.A on mRNA at high resolution from as little as 1?μg of poly(A)-selected mRNA.作者: needle 時(shí)間: 2025-3-27 15:56
Ewald Balcar,Stephen W. Loveseyn-reagent, as exemplified on a 110 nucleotide RNA obtained via in vitro transcription. The method exploits the fact that, under neutral buffer conditions (~pH 6.8), the 5′-phosphate carries the only mildly acidic proton in the RNA molecule, which allows for selective functionalization at that site using diazo reagents.作者: 同步左右 時(shí)間: 2025-3-27 18:24 作者: QUAIL 時(shí)間: 2025-3-28 01:08
Effects of Air Pollutants on Vegetation,bisulfite sequencing (RNA-BisSeq). Here we introduce an optimized RNA-BisSeq method by using ACT random hexamers to prime the reverse transcription of bisulfite-treated RNA samples to detect the m.C sites.作者: inspiration 時(shí)間: 2025-3-28 02:31 作者: Mettle 時(shí)間: 2025-3-28 07:41 作者: mendacity 時(shí)間: 2025-3-28 12:14 作者: 浮雕 時(shí)間: 2025-3-28 17:18
Energy Equilibrium and Nuclear Reactions,coding genes, LncVar. In this chapter, we describe the process of collecting data (including lncRNAs, transcription factor binding sites and m.A modification sites of lncRNAs, putatively translated open reading frames in lncRNAs) and steps of evaluating the effects of variations on the transcriptional regulation and modification of lncRNAs.作者: 螢火蟲 時(shí)間: 2025-3-28 21:01
https://doi.org/10.1007/978-94-011-9228-6gth isoforms from short read RNA-seq data. CIDANE bridges the gap between RNA quantification methods that rely on a complete annotation of a species’ transcriptome, and transcript assembly methods that will detect novel isoforms at the cost of a lower accuracy.作者: 脫水 時(shí)間: 2025-3-29 00:13
Larry A. Lambe,David E. Radfordt completely understood. Similarly, the mechanisms by which viral infections may alter the dynamics of the host RNA methylome have yet to be elucidated. Here, we describe an experimental protocol to obtain m.A profiles of ZIKV RNA and the host cell mRNA using methylated RNA immunoprecipitation–sequencing (MeRIP-Seq).作者: fallible 時(shí)間: 2025-3-29 04:07
A Model of the Language of Law,n promote tumorigenesis. Here, we present a mRNA expression analysis-based approach to comprehensively determine the expression of RNA readers/writers/erasers using DNA damage as an example, and then to validate the effect of altered RNA reader/writer/erasers in regulating the DNA damage response.作者: 相符 時(shí)間: 2025-3-29 11:18
Genome-Wide Annotation of circRNAs and Their Alternative Back-Splicing/Splicing with CIRCexplorer Plicing and alternative splicing events is the basis for the functional characterization of different categories of circRNAs. Here we describe a step-by-step computational scheme to annotate circRNAs from publicly available RNA sequencing datasets with the CIRCexplorer2 pipeline.作者: reptile 時(shí)間: 2025-3-29 14:16
,Synthesis and Evaluation of Novel Neamine–Nucleoside Conjugates as Potential Antibiotic Targets fore–amino acid substituted nucleoside conjugate . and . showed 6.3 and 4.8 times greater RNA binding affinity, respectively, as compared with neamine. The results obtained successfully demonstrate the need for chemically modifying neamine and probe the changes induced using NMR protocols to assist in the discovery of new aminoglycoside antibiotics.作者: Anticonvulsants 時(shí)間: 2025-3-29 18:00 作者: mortgage 時(shí)間: 2025-3-29 22:13
LncVar: Deciphering Genetic Variations Associated with Long Noncoding Genes,coding genes, LncVar. In this chapter, we describe the process of collecting data (including lncRNAs, transcription factor binding sites and m.A modification sites of lncRNAs, putatively translated open reading frames in lncRNAs) and steps of evaluating the effects of variations on the transcriptional regulation and modification of lncRNAs.作者: 極端的正確性 時(shí)間: 2025-3-30 00:19
Guided Reconstruction of Full-Length Isoforms from Short Reads by CIDANE,gth isoforms from short read RNA-seq data. CIDANE bridges the gap between RNA quantification methods that rely on a complete annotation of a species’ transcriptome, and transcript assembly methods that will detect novel isoforms at the cost of a lower accuracy.作者: single 時(shí)間: 2025-3-30 05:14 作者: 附錄 時(shí)間: 2025-3-30 11:01
RNA Modification Regulatory Genes in DNA Damage,n promote tumorigenesis. Here, we present a mRNA expression analysis-based approach to comprehensively determine the expression of RNA readers/writers/erasers using DNA damage as an example, and then to validate the effect of altered RNA reader/writer/erasers in regulating the DNA damage response.作者: 閑蕩 時(shí)間: 2025-3-30 12:53 作者: 脆弱帶來(lái) 時(shí)間: 2025-3-30 19:49
,Yee Algorithm for Maxwell’s Equations, to the target RNA sequence. Binding of the MBs to the tandem repeats could illuminate the target RNA as a bright spot when imaged by conventional fluorescence microscopy, making the MB-based imaging approach a versatile tool for RNA analysis across laboratories. In this chapter, we describe the dev作者: 法律 時(shí)間: 2025-3-30 21:06
https://doi.org/10.1007/978-94-010-1655-1eveloped web-based interfaces to analyze the associations between RNA modification sites with miRNA target sites and disease-related single-nucleotide polymorphisms (SNPs). Moreover, RMBase provides a genome browser and a web-based modTool to query, annotate, and visualize various RNA modifications.作者: 提名 時(shí)間: 2025-3-31 02:40
Non-wheeled Vehicles and Rovers,d mRNA fragments and their 3′-uridylation in human cells under physiological conditions. The SLA-RT-PCR methods have been demonstrated as a sensitive, cost-efficient, and high-throughput tool to systematically detect miRNA-targeted mRNA cleavage sites and fragments with 3′-uridylation in human cells作者: 6Applepolish 時(shí)間: 2025-3-31 08:07
The Main Formats of the SP Industry,st, the second method, based on site-specific cleavage/labeling, nuclease digestion, and TLC, is quantitative, but can be used to analyze only one site at a time. These two methods can be used independently or in combination.作者: Obverse 時(shí)間: 2025-3-31 10:02
1064-3745 aboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and comprehensive, .Epitranscriptomics: Methods and Protocols. is an important resource for both expert and novice scientists who are interested in learning more aboutthis field..978-1-4939-8808-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Common-Migraine 時(shí)間: 2025-3-31 15:55 作者: 扔掉掐死你 時(shí)間: 2025-3-31 21:22
Decoding the Atlas of RNA Modifications from Epitranscriptome Sequencing Data,eveloped web-based interfaces to analyze the associations between RNA modification sites with miRNA target sites and disease-related single-nucleotide polymorphisms (SNPs). Moreover, RMBase provides a genome browser and a web-based modTool to query, annotate, and visualize various RNA modifications.作者: CORD 時(shí)間: 2025-3-31 21:51
,Detection of MicroRNA-Mediated Target mRNA Cleavage and 3′-Uridylation in Human Cells by a SLA-RT-Pd mRNA fragments and their 3′-uridylation in human cells under physiological conditions. The SLA-RT-PCR methods have been demonstrated as a sensitive, cost-efficient, and high-throughput tool to systematically detect miRNA-targeted mRNA cleavage sites and fragments with 3′-uridylation in human cells作者: Galactogogue 時(shí)間: 2025-4-1 05:47 作者: 中子 時(shí)間: 2025-4-1 09:53
Springer Science+Business Media, LLC, part of Springer Nature 2019
