作者: Collar 時(shí)間: 2025-3-21 22:39 作者: 外來(lái) 時(shí)間: 2025-3-22 01:56 作者: EVADE 時(shí)間: 2025-3-22 07:30 作者: 爆米花 時(shí)間: 2025-3-22 10:30
A Simple Solid-Phase Competition Assay with Labeled Antigen, the antigen is immobilized, and a radiolabeled antibody and competing unlabeled antibodies are mixed in solution (.) (.,.). Although this method facilitates separation of free from bound antibody, it possesses the problem of labeling all antibodies to be tested. Since the number of MAbs to be scree作者: mucous-membrane 時(shí)間: 2025-3-22 12:57 作者: mucous-membrane 時(shí)間: 2025-3-22 19:01
Identifying Residues in Antigenic Determinants by Chemical Modification, proteolytic fragmentation, it played a major role in the pioneering efforts of Atassi and others to assign antigenic determinants on the surfaces of lysozyme and myoglobin (.,.). The principle of the method is that alteration of the structure of a key residue in an epitope by a chemical modificatio作者: 燕麥 時(shí)間: 2025-3-23 00:52
Epitope Mapping by Differential Chemical Modification of Antigens, in three-dimensional space. In the case of antibodies to native proteins, most or perhaps all epitopes are discontinuous (.). Because of the large size of a typical contact epitope in an antigen-antibody crystal, it is unlikely for an antibody to bind exclusively to a contiguous stretch of the poly作者: LAPSE 時(shí)間: 2025-3-23 05:21 作者: 是他笨 時(shí)間: 2025-3-23 07:24
Proteolytic Fragmentation for Epitope Mapping,dely used to provide antibodies with a defined specificity. One characteristic feature of this technology is that impure antigens can be used to produce monospecific antibodies that can be utilized to study the functional domains of protein molecules. In this chapter, the use of limited vs complete 作者: Asperity 時(shí)間: 2025-3-23 10:20
Epitope Mapping by Chemical Fragmentation,g fragments are screened with antibody on Western blots. It is widely applicable insofar as it is not restricted to recombinant antigens only, but the amino-acid sequence of the antigen must be known. It cannot be used for highly assembled epitopes, which will be affected by the denaturing condition作者: subordinate 時(shí)間: 2025-3-23 15:13 作者: Nonporous 時(shí)間: 2025-3-23 20:31
Epitope Mapping Using Multipin Peptide Synthesis,his protein? How long is the critical part of each epitope? Which epitopes are commonly recognized and which are rarely recognized? What are the affinities for each epitope? Which amino acids in the epitope are in contact with the antibody/TCR/MHC molecule? What variants of the epitope are still rec作者: ferment 時(shí)間: 2025-3-24 00:53
Tea Bag Synthesis of Positional Scanning Synthetic Combinatorial Libraries and Their Use for Mappind by monoclonal antibodies (MAbs). These libraries are typically composed, in total, of tens of millions of nonsupport-bound peptides, and can be readily screened using enzyme-linked immunosorbent assay (ELISA) to determine specific sequences that bind to a target antibody. From our studies using pe作者: Proponent 時(shí)間: 2025-3-24 05:29 作者: Melodrama 時(shí)間: 2025-3-24 06:33 作者: 表被動(dòng) 時(shí)間: 2025-3-24 14:32
Homolog Scanning,. They have thus become important tools for serotyping of viruses and bacteria (.–.), and for typing of homologous proteins from multigene families, such as subtypes of different human interferon molecules (.) or HLA markers (.).作者: 北京人起源 時(shí)間: 2025-3-24 17:58
Crystallographic Studies of Antigen-Antibody Interactions,n cases where structures of both liganded and unliganded forms of an antigen or antibody fragment are known, crystallographic studies have led to a more dynamic view of binding, often showing conformational changes (induced fit) in the antibody or antigen and displacement of water molecules at the binding interface.作者: Perceive 時(shí)間: 2025-3-24 19:02
Epitope Mapping Using Multipin Peptide Synthesis,ities for each epitope? Which amino acids in the epitope are in contact with the antibody/TCR/MHC molecule? What variants of the epitope are still recognized by the antibody/TCR/MHC molecule? Which peptides are antagonistic peptides for this epitope?作者: ORE 時(shí)間: 2025-3-24 23:19
https://doi.org/10.1007/978-1-4471-3903-4ase of tight binding and slow exchange, isotope filtering methods can be applied (.). Tsang et al. (.) used this technique to elucidate amide proton interactions in an antibody-bound peptide complex and to characterize the mobility of these protons on binding.作者: 卜聞 時(shí)間: 2025-3-25 04:21
Introduction to Time-Delay Systems used to determine the sequence of the fragments recognized by the MAb 4A. Therefore, reaction times and reagents presented in this chapter may require some modification when a different protein antigen is under investigation.作者: 無(wú)聊點(diǎn)好 時(shí)間: 2025-3-25 10:33 作者: 山崩 時(shí)間: 2025-3-25 15:41 作者: 挑剔小責(zé) 時(shí)間: 2025-3-25 18:11 作者: 的事物 時(shí)間: 2025-3-25 22:58
Proteolytic Fragmentation for Epitope Mapping, used to determine the sequence of the fragments recognized by the MAb 4A. Therefore, reaction times and reagents presented in this chapter may require some modification when a different protein antigen is under investigation.作者: rheumatology 時(shí)間: 2025-3-26 00:19 作者: 過(guò)時(shí) 時(shí)間: 2025-3-26 07:32 作者: angiography 時(shí)間: 2025-3-26 11:32
Book 1996o- gists from a wide range of disciplines in which antibodies are used as molecu- lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoimmune disea作者: 使更活躍 時(shí)間: 2025-3-26 14:00 作者: mortgage 時(shí)間: 2025-3-26 20:50
,Numerical Simulations and?Experiments, the chosen reagent, removal of byproducts after the reaction, and immunoassay of the modified protein for the expression of the epitope. The chemical modification is usually performed in solution, but in a convenient variation described here, the antigen is modified when it is already adsorbed to an ELISA plate (.).作者: Oration 時(shí)間: 2025-3-26 21:21
Introduction to Thin Film Transistorsof peptide release from the free antigen and from the antigen-MAb complex. By analogy to DNase footprinting, a method to localize protein-binding sites on DNA by nuclease digestion of protein-bound DNA (.), proteolysis of antigen-MAb complexes to identify epitopes on protein antigens has been referred to as “protein footprinting” (.).作者: 佛刊 時(shí)間: 2025-3-27 04:58
https://doi.org/10.1007/978-981-16-6092-4cyanogen bromide (CNBr) cleaves C-terminal to methionine residues (.), nitrothiocyanobenzoic acid (NTCB) at Cys (.), iodosobenzoic acid (IBA) at Trp (.), and formic acid between Asp-Pro bonds (.). The fragments on Western blots can often be recognized unequivocally using ..s predicted from the sequence.作者: 改革運(yùn)動(dòng) 時(shí)間: 2025-3-27 08:08 作者: Engulf 時(shí)間: 2025-3-27 13:22 作者: COW 時(shí)間: 2025-3-27 13:50
Identifying Residues in Antigenic Determinants by Chemical Modification, the chosen reagent, removal of byproducts after the reaction, and immunoassay of the modified protein for the expression of the epitope. The chemical modification is usually performed in solution, but in a convenient variation described here, the antigen is modified when it is already adsorbed to an ELISA plate (.).作者: Obvious 時(shí)間: 2025-3-27 19:28 作者: 一夫一妻制 時(shí)間: 2025-3-28 00:14
Epitope Mapping by Chemical Fragmentation,cyanogen bromide (CNBr) cleaves C-terminal to methionine residues (.), nitrothiocyanobenzoic acid (NTCB) at Cys (.), iodosobenzoic acid (IBA) at Trp (.), and formic acid between Asp-Pro bonds (.). The fragments on Western blots can often be recognized unequivocally using ..s predicted from the sequence.作者: 窩轉(zhuǎn)脊椎動(dòng)物 時(shí)間: 2025-3-28 02:07
Epitope Mapping Using Phage-Displayed Peptide Libraries,tein folding. These determinants are present on native protein, but are lost on denaturation or fragmentation. Linear or continuous epitopes contain at least four to six adjacent amino acid residues of the primary sequence and can be identified on denatured as well as native protein (.–.).作者: MIRE 時(shí)間: 2025-3-28 09:01
1064-3745 ed by biolo- gists from a wide range of disciplines in which antibodies are used as molecu- lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoim作者: 笨拙處理 時(shí)間: 2025-3-28 14:10 作者: 責(zé)難 時(shí)間: 2025-3-28 17:42
Introduction to Transfer Learningities for each epitope? Which amino acids in the epitope are in contact with the antibody/TCR/MHC molecule? What variants of the epitope are still recognized by the antibody/TCR/MHC molecule? Which peptides are antagonistic peptides for this epitope?作者: 緊張過(guò)度 時(shí)間: 2025-3-28 21:20 作者: JOT 時(shí)間: 2025-3-29 00:58
Book 1996esearch and most of them elected to do so. After an introductory chapter, the protocols are arranged in three groups: The first group of twelve methods uses (or can use) whole, native antigens and may therefore be suitable for conformational epitopes; the second group of five uses peptides or peptid作者: 歡樂(lè)東方 時(shí)間: 2025-3-29 05:58 作者: 驚惶 時(shí)間: 2025-3-29 07:19 作者: plasma 時(shí)間: 2025-3-29 14:28
Epitope Mapping by Surface Plasmon Resonance in the BIAcore,n matrix. Light covering a span of angles of incidence falls on the glass side and is reflected into a 2D array detector where the intensity of the reflected light is measured. SPR occurs at a certain angle and is seen as a minimum in reflected light intensity. When the refractive index close to the作者: 高調(diào) 時(shí)間: 2025-3-29 16:42
Epitope Mapping by Differential Chemical Modification of Antigens,mate location and outline of a protein epitope may be established by a combination of methods that can be applied to the antigen—antibody complex in solution. One such method is the differential chemical modification of the free and antibody-bound protein. Other methods complementing differential ch作者: Functional 時(shí)間: 2025-3-29 21:00 作者: 辯論的終結(jié) 時(shí)間: 2025-3-30 02:11
Insurance from the Shariah Perspective,n a protein surface that can elicit an antibody response, at the end of which one might claim to have produced an “epitope map” of the protein antigen (.). This information might be very useful, for example, to someone wishing to produce antiviral vaccines. Implicit in this view of epitopes is that 作者: 潔凈 時(shí)間: 2025-3-30 07:11 作者: meritorious 時(shí)間: 2025-3-30 09:06
Introduction to Thermal Analysisn matrix. Light covering a span of angles of incidence falls on the glass side and is reflected into a 2D array detector where the intensity of the reflected light is measured. SPR occurs at a certain angle and is seen as a minimum in reflected light intensity. When the refractive index close to the作者: 無(wú)脊椎 時(shí)間: 2025-3-30 14:31
The second law of thermodynamics,mate location and outline of a protein epitope may be established by a combination of methods that can be applied to the antigen—antibody complex in solution. One such method is the differential chemical modification of the free and antibody-bound protein. Other methods complementing differential ch作者: thrombus 時(shí)間: 2025-3-30 17:16 作者: POWER 時(shí)間: 2025-3-30 23:02
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/313383.jpg作者: ORE 時(shí)間: 2025-3-31 02:32
Epitope Mapping Protocols978-1-59259-552-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: CRASS 時(shí)間: 2025-3-31 07:02
https://doi.org/10.1007/978-3-642-76214-7Most epitope mapping techniques can be classified into two groups: those that use antigenic fragments (subunits, peptides, and so forth) and the specific antibody-binding regions are directly determined, and those that use intact antigen and the epitope determination is usually indirect.作者: lethal 時(shí)間: 2025-3-31 10:44 作者: exacerbate 時(shí)間: 2025-3-31 13:20
Epitope Mapping by Antibody Competition,Most epitope mapping techniques can be classified into two groups: those that use antigenic fragments (subunits, peptides, and so forth) and the specific antibody-binding regions are directly determined, and those that use intact antigen and the epitope determination is usually indirect.作者: conduct 時(shí)間: 2025-3-31 20:57
SPOT Synthesis,SPOT synthesis is an easy and very flexible technique for simultaneous parallel chemical synthesis on membrane supports. This method gives immunologists rapid and low-cost access to large numbers of peptides both as solid-phase-bound and solution-phase products for systematic epitope analysis.作者: enchant 時(shí)間: 2025-3-31 23:55
Homayoun Nikookar,Ramjee Prasad. They have thus become important tools for serotyping of viruses and bacteria (.–.), and for typing of homologous proteins from multigene families, such as subtypes of different human interferon molecules (.) or HLA markers (.).作者: Permanent 時(shí)間: 2025-4-1 03:32
Homolog Scanning,. They have thus become important tools for serotyping of viruses and bacteria (.–.), and for typing of homologous proteins from multigene families, such as subtypes of different human interferon molecules (.) or HLA markers (.).作者: charisma 時(shí)間: 2025-4-1 09:52 作者: enmesh 時(shí)間: 2025-4-1 12:38 作者: insomnia 時(shí)間: 2025-4-1 17:42 作者: inspiration 時(shí)間: 2025-4-1 19:13
THz Propagation and Related Topics,for purifying, binding, or labeling the antigen or the MAb. These procedures require some technical proficiency, may present methodological problems, and are based on much trial and error, but, worst of all, these manipulations, including the simple adsorption of molecules to surfaces, may induce th
