作者: 宿醉 時間: 2025-3-21 23:11 作者: 我們的面粉 時間: 2025-3-22 01:55 作者: 橫條 時間: 2025-3-22 07:22
The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eyring these cells from recently deceased adult human donors is the ideal source for disease studies. Adult RPE could also be used as a cell source for transplantation therapy for RPE degenerative disease, likely requiring first in vitro expansion of the cells obtained from a patient. Previous protoco作者: pancreas 時間: 2025-3-22 08:57
Oral and Pharyngeal Epithelial Keratinocyte Culture,ly utilized in multiple research efforts, ranging from the study of oral biology, mucosal immunity, and carcinogenesis to stem cell biology and tissue engineering. This chapter describes step-by-step protocols for the successful isolation and culture of human oral epithelial cells and fibroblasts, a作者: 母豬 時間: 2025-3-22 16:56 作者: 母豬 時間: 2025-3-22 20:55 作者: 過分自信 時間: 2025-3-22 21:12 作者: mitral-valve 時間: 2025-3-23 03:23 作者: Cholecystokinin 時間: 2025-3-23 09:16
Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat Lungs, lungs. The study of isolated and cultured alveolar epithelial TI and TII cells has provided a large amount of information about the functions of both cell types. This chapter provides information about methods for isolating and culturing both of these cell types from rat lungs.作者: minimal 時間: 2025-3-23 11:12 作者: conceal 時間: 2025-3-23 16:47
Mouse Epidermal Keratinocyte Culture,nsformation. Primary mouse keratinocytes can be isolated from newborn mice, and their growth and differentiation can be manipulated by changing calcium concentrations in culture medium. Primary mouse keratinocytes proliferate in medium containing 0.05 mM calcium but cease to proliferate and start to作者: 條街道往前推 時間: 2025-3-23 20:03 作者: 陶醉 時間: 2025-3-23 22:59 作者: 我就不公正 時間: 2025-3-24 02:44 作者: 不如樂死去 時間: 2025-3-24 06:46
Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development, but not undifferentiated stem cells, are mainly responsible for acute liver regeneration. Liver stem cells take part in liver regeneration in some forms of chronic liver injury, when the proliferative ability of differentiated hepatocytes is impaired. During liver development, both hepatocytes and 作者: carotid-bruit 時間: 2025-3-24 12:02
Long-Term Culture and Coculture of Primary Rat and Human Hepatocytes,nfection is a major cause of death in the United States. The development of Bioartificial Liver (BAL) devices and the demand for pharmaceutical and cosmetic toxicity screening require the development of long-term hepatocyte culture techniques. However, primary hepatocytes rapidly lose their cuboidal作者: FAZE 時間: 2025-3-24 16:29 作者: 背心 時間: 2025-3-24 20:06 作者: 極小量 時間: 2025-3-25 02:01 作者: CAGE 時間: 2025-3-25 07:24 作者: amphibian 時間: 2025-3-25 09:26 作者: indemnify 時間: 2025-3-25 11:59 作者: Irksome 時間: 2025-3-25 17:38 作者: 機(jī)警 時間: 2025-3-25 21:26
Human Nasal and Tracheo-Bronchial Respiratory Epithelial Cell Culture,ying basic biology, disease pathogenesis, gene therapy and aerosol administration of drugs. This chapter gives detailed protocols for tissue procurement, cell isolation, production of complex media, and cell culture initiation and maintenance needed for hAE cell ALI cultures with non-proprietary reagents.作者: 態(tài)學(xué) 時間: 2025-3-26 04:06
Mouse Epidermal Keratinocyte Culture,m concentrations in culture medium. Primary mouse keratinocytes proliferate in medium containing 0.05 mM calcium but cease to proliferate and start to terminally differentiate, when the calcium concentration in the culture medium is increased to 0.10 mM or greater.作者: 結(jié)束 時間: 2025-3-26 05:03
https://doi.org/10.1007/978-94-017-6312-7ing of potential therapeutic agents. The ensuing chapter describes several methods for primary culture of both corneal and conjunctival epithelia and culture of immortalized cell lines, and methods employed to induce differentiation in the cultured epithelia.作者: 哎呦 時間: 2025-3-26 09:48
Anglophone Literature of Caribbean Indenturend techniques for their use in two-dimensional and three-dimensional culture systems. The described methods will enable to generate reconstituted tissues that resemble epithelial like structures in vitro, which can recapitulate some of the key features of the oral epithelium in vivo.作者: 窩轉(zhuǎn)脊椎動物 時間: 2025-3-26 13:03
https://doi.org/10.1007/978-3-658-23783-7ying basic biology, disease pathogenesis, gene therapy and aerosol administration of drugs. This chapter gives detailed protocols for tissue procurement, cell isolation, production of complex media, and cell culture initiation and maintenance needed for hAE cell ALI cultures with non-proprietary reagents.作者: ENNUI 時間: 2025-3-26 17:43
How Charisma and Pathos Move Audiencese airways. Methods are also provided for manipulation of differentiation and analysis of differentiation and gene expression. These approaches allow the assessment of global responses and those of specific cell subpopulations within the airway epithelium.作者: 發(fā)現(xiàn) 時間: 2025-3-26 22:32
https://doi.org/10.1007/978-3-642-93245-8methods, providing guidance for isolation and serial cultivation of primary human keratinocytes and dermal fibroblasts, as well as the use of these cells types for generation of stratified skin tissue.作者: foliage 時間: 2025-3-27 04:18 作者: 煩擾 時間: 2025-3-27 06:13
Classical Human Epidermal Keratinocyte Cell Culture,methods, providing guidance for isolation and serial cultivation of primary human keratinocytes and dermal fibroblasts, as well as the use of these cells types for generation of stratified skin tissue.作者: 反對 時間: 2025-3-27 12:32
1064-3745 ation advice from the experts.Includes supplementary materia.Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g.,作者: Banquet 時間: 2025-3-27 16:13
Susanne Martin,Thomas Linpinselm concentrations in culture medium. Primary mouse keratinocytes proliferate in medium containing 0.05 mM calcium but cease to proliferate and start to terminally differentiate, when the calcium concentration in the culture medium is increased to 0.10 mM or greater.作者: contrast-medium 時間: 2025-3-27 19:18 作者: modest 時間: 2025-3-28 01:00
Anglo-Jewish Women Writing the Holocaust from rat choroid plexus tissue, and a similar immortalized murine choroidal epithelial cell culture known as Z310 cells has also been established. Both cultures display a dominant polygonal morphology, and immunochemical studies demonstrate the presence of transthyretin, a thyroxine transport prote作者: 混沌 時間: 2025-3-28 02:37 作者: Inkling 時間: 2025-3-28 06:46 作者: 信條 時間: 2025-3-28 12:04
,From Peace to War and Back (1991–2002),culture for more than seven passages, without loss of viability and with retention of the molecular and biochemical properties of acutely isolated taste cells. Cultured primary human fungiform papillae cells also exhibit functional responses to taste stimuli indicating the presence of taste receptor作者: Respond 時間: 2025-3-28 18:32
Eva-Maria Lewkowicz,Beate West-Leuery polarized luminal cells delineates ductal or acinar lumena at the apical pole. Luminal cells make contact with myoepithelial cells and, in certain areas at the basal pole, also with basement membrane (BM) components. In this chapter, we describe how this exquisite organization as well as stages of作者: ectropion 時間: 2025-3-28 19:36
Vorbemerkungen. Zur Einleitung,hat the microenvironment, including the extracellular matrix and tissue architecture, plays a crucial role in directing functional differentiation of organs. Thus, in order for culture systems to be effective experimental models, they need to recapitulate the basic unit of differentiated function in作者: 令人作嘔 時間: 2025-3-28 23:41 作者: grudging 時間: 2025-3-29 05:15
https://doi.org/10.1007/978-3-658-00845-1. cells (from adult livers) can form a colony containing both albumin-positive hepatocytes and cytokeratin 19-positive bile ductal cells, indicating that these cells have the characters of liver stem/progenitor cells (proliferative capability and bipotency for hepatic and for biliary epithelial diff作者: instulate 時間: 2025-3-29 07:19 作者: 等級的上升 時間: 2025-3-29 12:56
https://doi.org/10.1007/978-3-658-23859-9ahepatic bile ducts. We also identified the essential involvement of the MEK5-ERK5 pathway in the abnormal proliferation of BECs in the PCK rat. The degradation of laminin and type IV collagen (basal membrane components of bile ducts) was closely related to the biliary dysgenesis and cystogenesis in作者: TIGER 時間: 2025-3-29 18:56
Internationalisierung und Managementtestinal epithelium. Single isolated Lgr5+ intestinal stem cells also form these organoid structures, in which stem cells are maintained by self-renewal and give rise to all lineages of the intestinal epithelium. This culture system is particularly useful for studying the regulation of intestinal st作者: 大方一點(diǎn) 時間: 2025-3-29 20:40
,Culture of Choroid Plexus Epithelial Cells and In Vitro Model of Blood–CSF Barrier, from rat choroid plexus tissue, and a similar immortalized murine choroidal epithelial cell culture known as Z310 cells has also been established. Both cultures display a dominant polygonal morphology, and immunochemical studies demonstrate the presence of transthyretin, a thyroxine transport prote作者: 截斷 時間: 2025-3-30 00:13 作者: BAIL 時間: 2025-3-30 06:17
Primary Culture of the Human Olfactory Neuroepithelium,iple investigators. The protocol provides a consistent, heterogeneous cell population, which demonstrates functional responses to odorant mixtures and exhibits a complex neuronal phenotype, encompassing receptors and signaling pathways pertinent to both olfaction and other aspects of CNS function. T作者: 虛度 時間: 2025-3-30 12:16 作者: 內(nèi)向者 時間: 2025-3-30 14:04 作者: 正常 時間: 2025-3-30 17:43 作者: 無辜 時間: 2025-3-31 00:35 作者: nullify 時間: 2025-3-31 04:52 作者: overhaul 時間: 2025-3-31 05:06 作者: puzzle 時間: 2025-3-31 11:53
Tissue Culture Correlational Study of Genetic Cholangiopathy of Autosomal Recessive Polycystic Kidnahepatic bile ducts. We also identified the essential involvement of the MEK5-ERK5 pathway in the abnormal proliferation of BECs in the PCK rat. The degradation of laminin and type IV collagen (basal membrane components of bile ducts) was closely related to the biliary dysgenesis and cystogenesis in作者: Provenance 時間: 2025-3-31 13:30 作者: 束縛 時間: 2025-3-31 17:58 作者: 母豬 時間: 2025-4-1 01:42
https://doi.org/10.1007/978-3-476-05300-8 lungs. The study of isolated and cultured alveolar epithelial TI and TII cells has provided a large amount of information about the functions of both cell types. This chapter provides information about methods for isolating and culturing both of these cell types from rat lungs.作者: 沒收 時間: 2025-4-1 02:27
Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat Lungs, lungs. The study of isolated and cultured alveolar epithelial TI and TII cells has provided a large amount of information about the functions of both cell types. This chapter provides information about methods for isolating and culturing both of these cell types from rat lungs.作者: 明確 時間: 2025-4-1 09:41
https://doi.org/10.1007/978-1-62703-125-7cell systems; epithelial cells; epithelial interfaces; epithelial monolayers; keratinocyte culture; Cell 作者: 線 時間: 2025-4-1 11:39
978-1-4939-5679-1Springer Science+Business Media, LLC 2013作者: Platelet 時間: 2025-4-1 14:44
Epithelial Cell Culture Protocols978-1-62703-125-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
