作者: 前面 時(shí)間: 2025-3-21 20:45 作者: 搏斗 時(shí)間: 2025-3-22 01:13 作者: 大廳 時(shí)間: 2025-3-22 07:57
Differentiation of Gastrointestinal Cell Lines by Culture in Semi-wet Interface,n from the effects of other bodily functions, such as hormonal and immune responses. However, cells that do not form a tight epithelium, polarize or secrete mucins lack some of the important protection mechanisms intrinsic to epithelial cells in vivo, increasing their susceptibility to external agen作者: pineal-gland 時(shí)間: 2025-3-22 12:03
Three-Dimensional Cell Culture Model Utilization in Renal Carcinoma Cancer Stem Cell Research,s. The choice of proper culture media enables cancer stem cells proliferation in primary and stable cell lines. To obtain renal cell cancer stem-like phenotype, we employed media dedicated for mesenchymal cells and adult stem cells. Developed RCC cell line 3D culture system enables effective drug te作者: intercede 時(shí)間: 2025-3-22 16:36 作者: intercede 時(shí)間: 2025-3-22 20:26
Bovine Granulosa Cell Culture,l species investigated, cattle have become an excellent model for in vitro studies on follicular biology, both because of their resemblance with humans in terms of follicular biology and the importance of reproductive failure as a cause of lost productivity in the dairy industry. In this chapter, we作者: Diaphragm 時(shí)間: 2025-3-22 23:04 作者: cultivated 時(shí)間: 2025-3-23 01:32 作者: 美學(xué) 時(shí)間: 2025-3-23 06:51
3D Model Replicating the Intestinal Function to Evaluate Drug Permeability,other models are used in the beginning of any scientific discovery, the in vitro models. The relevance of in vitro cell based culture models for studying intestinal drug absorption and transcytosis during early stages of drug development is undeniable. Several in vitro co-culture models have been de作者: Certainty 時(shí)間: 2025-3-23 13:13
Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Prtments of the gastric pit-gland units mediate these functions. We successfully developed a method for the isolation and growth of normal human gastric epithelial cells using biopsies or surgically resected tissues as the source of the cells. Gastric epithelial cell aggregates were released from the作者: 從屬 時(shí)間: 2025-3-23 17:01 作者: Omnipotent 時(shí)間: 2025-3-23 21:03 作者: 王得到 時(shí)間: 2025-3-23 23:11
Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoidsse. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids作者: lipids 時(shí)間: 2025-3-24 03:35 作者: Adherent 時(shí)間: 2025-3-24 07:15
Murine and Human Mammary Cancer Cell Lines: Functional Tests,ted from the tumor, cells are subjected to multiple tests that dissect their ability to growth, migrate, degrade the surrounding stroma, produce 3-dimensional structures and differentiate. Targeted inhibitors, when added to these tests, are used to unravel how specific growth factors, receptors, and作者: AGONY 時(shí)間: 2025-3-24 14:14 作者: 珊瑚 時(shí)間: 2025-3-24 16:10
Primary Cultures of Olfactory Neurons from the Avian Olfactory Epithelium,ut the adult lifespan. Structurally and functionally mature olfactory neurons are generated in vitro from non-neuronal precursors in a process that resembles the in vivo counterparts. This chapter describes a technique for culture of olfactory neurons from the avian olfactory epithelium of embryonic作者: META 時(shí)間: 2025-3-24 19:37 作者: 舊式步槍 時(shí)間: 2025-3-25 02:21 作者: AROMA 時(shí)間: 2025-3-25 04:50
Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids, and (2) single cells dissociated from intact organoids.作者: Assault 時(shí)間: 2025-3-25 08:53 作者: figurine 時(shí)間: 2025-3-25 14:54
Vascular Injuries of the Upper Extremity, chickens; this enables the controlled laboratory study of a critical sensory system that is unstudied in this major vertebrate class. The techniques described here are broadly applicable to other endothermic vertebrate species.作者: 冷漠 時(shí)間: 2025-3-25 18:11 作者: 不舒服 時(shí)間: 2025-3-25 22:29 作者: 價(jià)值在貶值 時(shí)間: 2025-3-26 00:43
Hellmut G. Augustin-Voss,Bendicht U. Pauliccessibility to the follicular space, presenting a challenge in studying transepithelial movements. This limitation can be overcome by studying thyrocytes grown as two-dimensional cultures. Herein we present methods for isolation of thyroid follicles from juvenile pigs and preparation of high-resistance, polarized cultures.作者: Deadpan 時(shí)間: 2025-3-26 04:21 作者: echnic 時(shí)間: 2025-3-26 09:44 作者: ABYSS 時(shí)間: 2025-3-26 13:30
Amniotic Epithelial Cell Culture,, thus improving their?in vivo therapeutic potential and clinical outcomes. Here is described an improved oAEC cultural protocol able to preserve the native epithelial phenotype also after the in vitro amplification.作者: 烤架 時(shí)間: 2025-3-26 18:55 作者: 闡釋 時(shí)間: 2025-3-26 23:42
https://doi.org/10.1007/978-1-4614-8069-3 granulosa cells in an undifferentiated state and inducing their luteinization in vitro, and for the efficient transfection of granulosa cells with oligonucleotide sequences for the purpose of investigating the function of specific genes in vitro.作者: 野蠻 時(shí)間: 2025-3-27 03:57 作者: 單獨(dú) 時(shí)間: 2025-3-27 05:24
Bovine Granulosa Cell Culture, granulosa cells in an undifferentiated state and inducing their luteinization in vitro, and for the efficient transfection of granulosa cells with oligonucleotide sequences for the purpose of investigating the function of specific genes in vitro.作者: transplantation 時(shí)間: 2025-3-27 13:07 作者: 流浪 時(shí)間: 2025-3-27 14:15
Nikos E. Tsopanoglou,Michael E. Maragoudakisth a doubling time of 72–94?h and retain functional characteristics for 9–12 doublings. We have used them successfully in studies to elucidate the signaling by thyrotropin (TSH) and insulin-like growth factor 1.作者: sigmoid-colon 時(shí)間: 2025-3-27 18:04 作者: breadth 時(shí)間: 2025-3-27 22:52
https://doi.org/10.1007/978-3-7643-7650-5epithelial primary cultures containing mucus cells, neck cells, parietal cells, chief cells, and gastrin-secreting endocrine cells. This simple and convenient method will prove useful in the isolation of normal human gastric mucous epithelial cells for in vitro studies of gastric epithelial biology.作者: Forsake 時(shí)間: 2025-3-28 04:02
Angiogenesis and Nonmelanoma Skin Cancer,cryopreservation and defining characteristics of these intestinal organoids also make this work relevant for the advancement of epithelial organoid-based therapeutic technologies by allowing the production of large numbers of cells for use in clinical applications.作者: 帶來(lái) 時(shí)間: 2025-3-28 07:06 作者: Optometrist 時(shí)間: 2025-3-28 13:36 作者: Offset 時(shí)間: 2025-3-28 15:34
Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Pepithelial primary cultures containing mucus cells, neck cells, parietal cells, chief cells, and gastrin-secreting endocrine cells. This simple and convenient method will prove useful in the isolation of normal human gastric mucous epithelial cells for in vitro studies of gastric epithelial biology.作者: 寒冷 時(shí)間: 2025-3-28 22:28
Long-Term Culture of Intestinal Organoids,cryopreservation and defining characteristics of these intestinal organoids also make this work relevant for the advancement of epithelial organoid-based therapeutic technologies by allowing the production of large numbers of cells for use in clinical applications.作者: 暫時(shí)過(guò)來(lái) 時(shí)間: 2025-3-29 02:36 作者: 膝蓋 時(shí)間: 2025-3-29 04:17 作者: Mitigate 時(shí)間: 2025-3-29 09:23 作者: 樂(lè)器演奏者 時(shí)間: 2025-3-29 14:25
https://doi.org/10.1007/978-3-319-28140-7 intestinal mucosa requires therefore further investigation, particularly focused in addressing the abovementioned unmet needs. 3D models came as bridge between the in vitro and in vivo models. These models are proven to be influential of the drug effect in cells, being the most adequate to mimic th作者: ethnology 時(shí)間: 2025-3-29 15:37 作者: Mawkish 時(shí)間: 2025-3-29 21:05
Auguste Wackenheim M.D.,Jean-Paul Braun M.D. vivo characteristics, such as different cell types and complex tissue architecture. This in vitro (ex vivo. organ culture protocol of the swine large intestine aims at maintaining viable colonic mucosa for up to 5?days. The protocol described herein applies a combination of methods used for immorta作者: 教唆 時(shí)間: 2025-3-30 02:12
Reassembly of Functional Human Stem/Progenitor Cells in 3D Culture,diation xerostomia using a “bottom up” reassembly paradigm. We use specialized extracellular matrix and growth factor supplemented hyaluronate hydrogels to promote reassembly of human salivary stem/progenitor cells (hS/PCs) isolated after surgical resection, a method we describe in this chapter. Cel作者: 健談 時(shí)間: 2025-3-30 07:36 作者: FLIRT 時(shí)間: 2025-3-30 10:39 作者: Cuisine 時(shí)間: 2025-3-30 14:08 作者: CHAFE 時(shí)間: 2025-3-30 18:08 作者: 聽(tīng)覺(jué) 時(shí)間: 2025-3-30 20:51 作者: 詩(shī)集 時(shí)間: 2025-3-31 03:57
https://doi.org/10.1007/978-1-4939-8600-2cancer mammary cell lines; transcriptome; Pig blastocyst culture; Pancreatic bio-Scaffold; cancer stem c作者: innovation 時(shí)間: 2025-3-31 08:09
Matthias Kirsch,Peter McL. Blackum alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.作者: 鬼魂 時(shí)間: 2025-3-31 12:09 作者: 是突襲 時(shí)間: 2025-3-31 16:44 作者: innate 時(shí)間: 2025-3-31 19:57
