標(biāo)題: Titlebook: Epigenome Editing; Methods and Protocol Albert Jeltsch,Marianne G. Rots Book 2018 Springer Science+Business Media, LLC, part of Springer Na [打印本頁] 作者: CAP 時(shí)間: 2025-3-21 19:03
書目名稱Epigenome Editing影響因子(影響力)
書目名稱Epigenome Editing影響因子(影響力)學(xué)科排名
書目名稱Epigenome Editing網(wǎng)絡(luò)公開度
書目名稱Epigenome Editing網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Epigenome Editing被引頻次
書目名稱Epigenome Editing被引頻次學(xué)科排名
書目名稱Epigenome Editing年度引用
書目名稱Epigenome Editing年度引用學(xué)科排名
書目名稱Epigenome Editing讀者反饋
書目名稱Epigenome Editing讀者反饋學(xué)科排名
作者: 制定 時(shí)間: 2025-3-21 22:56
An Anthropological Study of Hospitalitypatiotemporal control. Nearly any brain region of interest can be targeted in rodents at every stage of postnatal life. Owing to the versatility, reproducibility, and utility of this technique, it is an important method for any laboratory interested in studying the cellular, circuit, and behavioral consequences of in vivo neuroepigenome editing.作者: 聲音刺耳 時(shí)間: 2025-3-22 04:18
Social Designation of Cultural Markers,rget the epitope of interest. The DNA is released from the proteins by heat and protease treatment, followed by degradation of contaminating RNAs with RNase. The resulting DNA is analyzed using various methods, including PCR, qPCR, or sequencing. This protocol outlines each of these steps for both yeast and human cells.作者: bronchiole 時(shí)間: 2025-3-22 07:38
Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniquespatiotemporal control. Nearly any brain region of interest can be targeted in rodents at every stage of postnatal life. Owing to the versatility, reproducibility, and utility of this technique, it is an important method for any laboratory interested in studying the cellular, circuit, and behavioral consequences of in vivo neuroepigenome editing.作者: landfill 時(shí)間: 2025-3-22 12:36 作者: hypotension 時(shí)間: 2025-3-22 13:51 作者: hypotension 時(shí)間: 2025-3-22 18:42 作者: 卜聞 時(shí)間: 2025-3-22 21:45 作者: 啞巴 時(shí)間: 2025-3-23 04:51 作者: defray 時(shí)間: 2025-3-23 07:11 作者: 新娘 時(shí)間: 2025-3-23 11:32 作者: UNT 時(shí)間: 2025-3-23 16:16 作者: 滋養(yǎng) 時(shí)間: 2025-3-23 19:11 作者: intoxicate 時(shí)間: 2025-3-23 22:41 作者: 秘方藥 時(shí)間: 2025-3-24 06:16 作者: Tinea-Capitis 時(shí)間: 2025-3-24 10:29 作者: 錯(cuò)誤 時(shí)間: 2025-3-24 12:32
Zinc Fingers, TALEs, and CRISPR Systems: A Comparison of Tools for Epigenome Editing researchers to functionally manipulate DNA, reconfigure chromatin structure, and ultimately reshape gene expression patterns. Epigenetic editing tools provide the ability to interrogate the relationship between epigenetic modifications and gene expression. Importantly, this information can be explo作者: 羅盤 時(shí)間: 2025-3-24 16:28 作者: Misgiving 時(shí)間: 2025-3-24 21:24 作者: TIGER 時(shí)間: 2025-3-25 02:49 作者: fibula 時(shí)間: 2025-3-25 05:55
Allele-Specific Epigenome Editingrect epigenetic editors to virtually any genomic site. This advancement in DNA-targeting technology brings allele-specific epigenome editing into reach, a “super-specific” variation of epigenome editing whose goal is an alteration of chromatin marks at only one selected allele of the target genomic 作者: palliative-care 時(shí)間: 2025-3-25 09:58
Key to Delivery: The (Epi-)genome Editing Vector Toolboxpy. For this, a functional copy of the gene in question together with elements controlling its expression is produced as a vector and introduced ex vivo into the patient’s own cells that subsequently are reinfused. Alternatively, vectors are administered directly in vivo. Although this strategy resu作者: deforestation 時(shí)間: 2025-3-25 12:39
CRISPR/dCas9 Switch Systems for Temporal Transcriptional Controlase-deactivated Cas9 (dCas9) provide scientists with tools to modulate transcription of genomic loci at will by targeting transcriptional effector domains. To interrogate the temporal order of events during transcriptional regulation, rapidly inducible CRISPR/dCas9 systems provide previously unmet m作者: 光明正大 時(shí)間: 2025-3-25 17:19
Delivery of Designer Epigenome Modifiers into Primary Human T Cellsies toward further understanding how gene expression is regulated and opportunities to harness these properties for therapeutic purposes. In contrast to gene knockout strategies, targeted epigenome modifications, such as editing of DNA methylation, can mediate gene expression modulation without chan作者: 拒絕 時(shí)間: 2025-3-25 22:38 作者: Aromatic 時(shí)間: 2025-3-26 00:50
Stable Expression of Epigenome Editors via Viral Delivery and Genomic IntegrationA-binding proteins. However, the delivery of plasmid DNA to express these fusion proteins via conventional transient transfection has certain consequences which need to be considered during the experimental design. Transient transfection achieves peak gene expression between 24 and 96?h post-transfe作者: 火車車輪 時(shí)間: 2025-3-26 07:30 作者: muffler 時(shí)間: 2025-3-26 08:32
Non-viral Methodology for Efficient Co-transfectionand functions of the genomic elements and epigenome modifications in an endogenous context and as new methods for treatment of diseases. Application of such technologies has drawn attention, however, to the prevailing lack of effective delivery methods. Promising viral and non-viral methods both cur作者: 聽覺 時(shí)間: 2025-3-26 14:08
Chromatin Immunoprecipitation in Human and Yeast Cellszation of transcription factors and posttranslational modification of histones. DNA and proteins are reversibly and covalently crosslinked using formaldehyde. Then the cells are lysed to release the chromatin. The chromatin is fragmented into smaller sizes either by micrococcal nuclease (MNase) or s作者: 陰險(xiǎn) 時(shí)間: 2025-3-26 20:42 作者: Rotator-Cuff 時(shí)間: 2025-3-26 23:15 作者: Chronic 時(shí)間: 2025-3-27 01:15 作者: 身體萌芽 時(shí)間: 2025-3-27 07:14 作者: KIN 時(shí)間: 2025-3-27 10:42 作者: 撤退 時(shí)間: 2025-3-27 14:51
Medieval Europe: Latin and Macaronic,ive DNA repair, or they are used as programmable DNA-binding units for positioning epigenome-modifying domains to predefined target sequences. However, for reaching its full potential, these components need to be delivered into cells in an efficient and safe manner. Here, we summarize current viral 作者: 定點(diǎn) 時(shí)間: 2025-3-27 20:09
Anna Amelina,Kenneth Horvath,Bruno Meeus9 enhancements. Specifically we provide details on their flaws and strengths and on the differences in molecular design between the switch systems. With this we aim to provide a selection guide for researchers with keen interest in rapid temporal control over transcriptional modulation through the C作者: muscle-fibers 時(shí)間: 2025-3-28 01:56
An Anthropological Journey into Well-Beinglected reagents in the target cells. Here, we describe a detailed protocol, for safe delivery of TALE-based designer epigenome modifiers in the form of in vitro transcribed mRNA into primary human CD4. T cells to efficiently silence the expression of an exemplary human gene (i.e., .).作者: 整體 時(shí)間: 2025-3-28 04:38 作者: remission 時(shí)間: 2025-3-28 07:36 作者: WAG 時(shí)間: 2025-3-28 14:28 作者: 厚顏無恥 時(shí)間: 2025-3-28 15:41 作者: 可能性 時(shí)間: 2025-3-28 19:06 作者: dandruff 時(shí)間: 2025-3-29 01:33
Key to Delivery: The (Epi-)genome Editing Vector Toolboxive DNA repair, or they are used as programmable DNA-binding units for positioning epigenome-modifying domains to predefined target sequences. However, for reaching its full potential, these components need to be delivered into cells in an efficient and safe manner. Here, we summarize current viral 作者: Sarcoma 時(shí)間: 2025-3-29 04:17 作者: 鋪?zhàn)?nbsp; 時(shí)間: 2025-3-29 08:11 作者: sebaceous-gland 時(shí)間: 2025-3-29 11:56 作者: 自然環(huán)境 時(shí)間: 2025-3-29 17:45 作者: 是限制 時(shí)間: 2025-3-29 20:46 作者: 護(hù)身符 時(shí)間: 2025-3-30 02:31
Albert Jeltsch,Marianne G. RotsIncludes cutting-edge techniques for the study of epigenome editing.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 敵意 時(shí)間: 2025-3-30 07:53
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/313256.jpg作者: 呼吸 時(shí)間: 2025-3-30 09:50
978-1-4939-9281-2Springer Science+Business Media, LLC, part of Springer Nature 2018作者: 云狀 時(shí)間: 2025-3-30 15:03 作者: 使絕緣 時(shí)間: 2025-3-30 17:11 作者: CODA 時(shí)間: 2025-3-30 21:48 作者: APNEA 時(shí)間: 2025-3-31 02:08
