作者: monogamy 時(shí)間: 2025-3-21 22:24
https://doi.org/10.1007/978-3-322-80801-1R subsequent to stimulation with the receptor ligand EGF is described. Finally, protocols to quantitatively measure degradation of the EGFR by pulse chase experiments or using radiolabeled ligands such as .I-EGF are presented.作者: 隱藏 時(shí)間: 2025-3-22 02:38 作者: patriarch 時(shí)間: 2025-3-22 04:40
Assays to Monitor Degradation of the EGF Receptor,R subsequent to stimulation with the receptor ligand EGF is described. Finally, protocols to quantitatively measure degradation of the EGFR by pulse chase experiments or using radiolabeled ligands such as .I-EGF are presented.作者: Costume 時(shí)間: 2025-3-22 12:38
1064-3745 ctions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.978-1-61737-557-6978-1-59745-012-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 免除責(zé)任 時(shí)間: 2025-3-22 15:20 作者: 免除責(zé)任 時(shí)間: 2025-3-22 18:34 作者: 疼死我了 時(shí)間: 2025-3-22 23:07 作者: muster 時(shí)間: 2025-3-23 04:45 作者: 泥瓦匠 時(shí)間: 2025-3-23 08:54
In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases, degradation. In this chapter we describe two methods that contrast polyubiquitination with monoubiquitination of EGFR. Because monoubiquitination enables evasion from proteasomal degradation, the methods we describe may be useful for the analysis of other monoubiquitination events.作者: GROG 時(shí)間: 2025-3-23 12:20
Methods for Determining the Proliferation of Cells in Response to EGFR Ligands, equipment available, the experimental design, and the questions being addressed. In this chapter we will describe methods for the use of a hemocytometer, [H.]-thymidine incorporation, cell cycle analysis with propidium iodide by flow cytometry, and evaluation of cellular metabolic activity with the MTS reagent.作者: canonical 時(shí)間: 2025-3-23 16:16 作者: 同義聯(lián)想法 時(shí)間: 2025-3-23 20:55
1064-3745 factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of thei作者: Axon895 時(shí)間: 2025-3-23 23:10 作者: 六邊形 時(shí)間: 2025-3-24 05:43
,Erkl?rungsans?tze strategischer Allianzen, these events requires an understanding of the controls of EGFR-mediated motility. Deconstruction of motility into its component events enables this deeper insight. Herein we describe methods that measure the overall motility and its parameters as well as the biophysical processes extension, de-adhesion/ retraction, and contraction.作者: Synchronism 時(shí)間: 2025-3-24 06:46 作者: 語(yǔ)源學(xué) 時(shí)間: 2025-3-24 13:17
Motility Signaled From the EGF Receptor and Related Systems, these events requires an understanding of the controls of EGFR-mediated motility. Deconstruction of motility into its component events enables this deeper insight. Herein we describe methods that measure the overall motility and its parameters as well as the biophysical processes extension, de-adhesion/ retraction, and contraction.作者: 迎合 時(shí)間: 2025-3-24 18:48
Typologies of Allies and Alliancesproteolytic processing of EGFR ligands. This chapter describes a sensitive and semiquantitative method to measure ectodomain shedding of EGFR ligands that was designed to facilitate studies of this process in cells.作者: 忍受 時(shí)間: 2025-3-24 20:46
A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptoproteolytic processing of EGFR ligands. This chapter describes a sensitive and semiquantitative method to measure ectodomain shedding of EGFR ligands that was designed to facilitate studies of this process in cells.作者: Overstate 時(shí)間: 2025-3-25 01:24
Book 2006ow the successful Methods in Molecular Biology? series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.作者: 枯萎將要 時(shí)間: 2025-3-25 04:49
https://doi.org/10.1007/978-3-8350-9505-2y EGF or other high-affinity ligands often results in increased migration of cells in physiological and pathological situations. There are numerous approaches for evaluating cell migratory response following EGF stimulation. Both qualitative and quantitative techniques will be presented in this chapter.作者: agglomerate 時(shí)間: 2025-3-25 10:24
https://doi.org/10.1057/9780230348936 drugs have been developed that are progressing rapidly in oncology clinical trials. This chapter provides an overview of EGFR inhibitors and key clinical trial results that are helping to define a future role for these molecular agents in cancer treatment.作者: Infant 時(shí)間: 2025-3-25 15:17
Epithelial Cell Migration in Response to Epidermal Growth Factor,y EGF or other high-affinity ligands often results in increased migration of cells in physiological and pathological situations. There are numerous approaches for evaluating cell migratory response following EGF stimulation. Both qualitative and quantitative techniques will be presented in this chapter.作者: 擴(kuò)音器 時(shí)間: 2025-3-25 17:42
Clinical Advancement of EGFR Inhibitors in Cancer Therapy, drugs have been developed that are progressing rapidly in oncology clinical trials. This chapter provides an overview of EGFR inhibitors and key clinical trial results that are helping to define a future role for these molecular agents in cancer treatment.作者: STIT 時(shí)間: 2025-3-25 21:58 作者: 善變 時(shí)間: 2025-3-26 03:31
Tarun B. Patel,Paul J. BerticsIncludes supplementary material: 作者: 不能約 時(shí)間: 2025-3-26 07:11 作者: 晚來(lái)的提名 時(shí)間: 2025-3-26 08:50
https://doi.org/10.1007/978-3-662-39569-1been shown to have various effects on numerous cellular systems. The biological and physiological role that EGF plays during development and in adult animals led to the identification of its receptor (EGFR) as well as the other members of the EGF family of growth factors and their receptors. In this作者: 有花 時(shí)間: 2025-3-26 14:21
Die Behandlung in der Allgemeinmedizin,ve been associated with many types of cancers. Therefore, understanding the mechanisms that modulate receptor activity and function is essential to the development of treatments for many of these cancers. However, to address this issue by either conventional or high-throughput screening methods requ作者: Processes 時(shí)間: 2025-3-26 20:28 作者: 滔滔不絕地說(shuō) 時(shí)間: 2025-3-27 00:46
https://doi.org/10.1007/978-3-319-49619-1uch as the heart, partotid gland, and luteal cells, activation of the epidermal growth factor (EGF) receptor also stimulates second-messenger systems that involve the heterotrimeric G proteins. For instance, in the heart EGF increases contractility and heart rate by elevating cellular cyclic adenosi作者: Monocle 時(shí)間: 2025-3-27 04:11
https://doi.org/10.1057/9780230505780 expression is also induced by the epidermal growth factor receptor (EGFR) cascade in some tissues, including the follicle cells of the ovary, the wing, and eye imaginal disc, and acts to abolish mitogen-activated protein (MAP) kinase activated by EGFR signaling. Sprouty is an intracellular protein 作者: 束縛 時(shí)間: 2025-3-27 09:19
https://doi.org/10.1007/978-3-030-37258-3activation represents the so far best investigated cross-talk mechanism comprising heterogeneous receptor families. In this signaling process G protein-coupled receptor (GPCR) stimulation induces phosphorylation of the EGFR, combining the broad diversity of GPCRs with the potent signaling capacities作者: 帽子 時(shí)間: 2025-3-27 12:35 作者: 離開真充足 時(shí)間: 2025-3-27 14:50
https://doi.org/10.1007/978-981-15-2533-9d molecule at the cell surface. Desensitization of signaling initiates when active receptors are recruited to clathrin-coated regions of the plasma membrane and subsequently sorted to intracellular degradation in lysosomes. Sorting for lysosomal degradation entails receptor conjugation with ubiquiti作者: 法律 時(shí)間: 2025-3-27 20:43
https://doi.org/10.1007/978-3-322-80801-1s followed by their internalization and degradation has, in particular, been extensively studied. This chapter describes the basic methods used to measure ubiquitination and degradation of RTKs using the example of the epidermal growth factor receptor (EGFR). Common sources for endogenous and recomb作者: Fracture 時(shí)間: 2025-3-28 00:44 作者: 好忠告人 時(shí)間: 2025-3-28 02:53
https://doi.org/10.1007/978-3-8350-9505-2y EGF or other high-affinity ligands often results in increased migration of cells in physiological and pathological situations. There are numerous approaches for evaluating cell migratory response following EGF stimulation. Both qualitative and quantitative techniques will be presented in this chap作者: 平 時(shí)間: 2025-3-28 08:01 作者: 有危險(xiǎn) 時(shí)間: 2025-3-28 13:05 作者: Rinne-Test 時(shí)間: 2025-3-28 14:58
https://doi.org/10.1057/9780230348936 drugs have been developed that are progressing rapidly in oncology clinical trials. This chapter provides an overview of EGFR inhibitors and key clinical trial results that are helping to define a future role for these molecular agents in cancer treatment.作者: Moderate 時(shí)間: 2025-3-28 19:54
Epidermal Growth Factor978-1-59745-012-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 原始 時(shí)間: 2025-3-29 02:21
A Historical Perspective of the EGF Receptor and Related Systems,been shown to have various effects on numerous cellular systems. The biological and physiological role that EGF plays during development and in adult animals led to the identification of its receptor (EGFR) as well as the other members of the EGF family of growth factors and their receptors. In this作者: 平庸的人或物 時(shí)間: 2025-3-29 03:47 作者: grandiose 時(shí)間: 2025-3-29 08:11
Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay,nked immunosorbent assay (ALISA) developed by Baron and coworkers (.). This ALISA has been shown to be specific for epidermal growth factor receptor (EGFR) and the isoforms of EGFR encoded by alternate transcripts (i.e., sEGFRs); it, therefore, does not detect other EGFR/ErbB receptor family members作者: 不自然 時(shí)間: 2025-3-29 13:55
,Analysis of EGF Receptor Interactions With the α Subunit of the Stimulatory GTP Binding Protein of uch as the heart, partotid gland, and luteal cells, activation of the epidermal growth factor (EGF) receptor also stimulates second-messenger systems that involve the heterotrimeric G proteins. For instance, in the heart EGF increases contractility and heart rate by elevating cellular cyclic adenosi作者: Gyrate 時(shí)間: 2025-3-29 17:39
Regulator of Epidermal Growth Factor Signaling, expression is also induced by the epidermal growth factor receptor (EGFR) cascade in some tissues, including the follicle cells of the ovary, the wing, and eye imaginal disc, and acts to abolish mitogen-activated protein (MAP) kinase activated by EGFR signaling. Sprouty is an intracellular protein 作者: FLIRT 時(shí)間: 2025-3-29 20:06
Dissecting the Epidermal Growth Factor Receptor Signal Transactivation Pathway,activation represents the so far best investigated cross-talk mechanism comprising heterogeneous receptor families. In this signaling process G protein-coupled receptor (GPCR) stimulation induces phosphorylation of the EGFR, combining the broad diversity of GPCRs with the potent signaling capacities作者: 多山 時(shí)間: 2025-3-30 01:14
A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Recepto from the plasma membrane. This process, which is referred to as protein ectodomain shedding, is emerging as a key regulator of the function of EGFR ligands. In light of the important roles of EGFR signaling in development and disease, it will be important to understand more about the regulation of
