標(biāo)題: Titlebook: Epidermal Cells; Methods and Protocol Kursad Turksen Book 2010Latest edition Humana Press 2010 DNA.Endoplasmatisches Reticulum.Fische.Micro [打印本頁(yè)] 作者: Helmet 時(shí)間: 2025-3-21 19:34
書目名稱Epidermal Cells影響因子(影響力)
書目名稱Epidermal Cells影響因子(影響力)學(xué)科排名
書目名稱Epidermal Cells網(wǎng)絡(luò)公開度
書目名稱Epidermal Cells網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Epidermal Cells被引頻次
書目名稱Epidermal Cells被引頻次學(xué)科排名
書目名稱Epidermal Cells年度引用
書目名稱Epidermal Cells年度引用學(xué)科排名
書目名稱Epidermal Cells讀者反饋
書目名稱Epidermal Cells讀者反饋學(xué)科排名
作者: 軍械庫(kù) 時(shí)間: 2025-3-22 00:09
Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Mse primitive keratinocytes give rise to the progenitors, which are the proliferating keratinocytes and which can be defined as early to late progenitors, according to their differentiation status. Because of the intrinsic heterogeneity of the basal layer, the development of new methods suitable for 作者: 是限制 時(shí)間: 2025-3-22 02:56 作者: Optometrist 時(shí)間: 2025-3-22 04:57 作者: considerable 時(shí)間: 2025-3-22 10:15 作者: Vldl379 時(shí)間: 2025-3-22 13:52 作者: Vldl379 時(shí)間: 2025-3-22 18:41 作者: 散布 時(shí)間: 2025-3-22 21:33
Expression and Analysis of Exogenous Proteins in Epidermal Cells,f their differentiation status allows the biochemical and molecular characterization of multiple proteins. We review methods to analyze exogenous protein abundance in transfected keratinocytes by immunoblot and immunoprecipitation. We also present protocols to determine the subcellular distribution 作者: 彎曲的人 時(shí)間: 2025-3-23 01:22 作者: Debark 時(shí)間: 2025-3-23 07:00 作者: 得罪人 時(shí)間: 2025-3-23 10:57
Scanning for Transcription Factor Binding by a Variant EMSA,rophoretic mobility shift assay (EMSA) has proven widely popular in this respect by integrating individual techniques (protein isolation, nucleic acid radiolabeling, and gel electrophoresis) into one protocol. However, relatively short DNA oligomers are often used which in many cases presupposes wha作者: 細(xì)胞 時(shí)間: 2025-3-23 14:36
Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes,ation of DNA sequences that interact with transcriptional regulatory proteins is an important step necessary to better understand the molecular mechanisms regulating gene expression. Chromatin immunoprecipitation (ChIP) is one such procedure that provides a snapshot of which transcription factors ar作者: HAIRY 時(shí)間: 2025-3-23 21:45 作者: 傳染 時(shí)間: 2025-3-24 00:55
Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection,d by its distance to the basal membrane. For that reason Laser Capture Microdissection (LCM) may serve as a perfect tool to compare the characteristics of cells that have been collected from different . of the epithelium. However, as cell boundaries are not visible in untreated tissue sections, samp作者: Relinquish 時(shí)間: 2025-3-24 04:17 作者: 長(zhǎng)矛 時(shí)間: 2025-3-24 07:58
Molecular Profiling of the Epidermis: A Proteomics Approach,ar mechanisms by which chemical carcinogens or tumor promoters cause the growth of cutaneous malignancies. In this regard, many transgenic or knockout mouse models have been generated in order to have a particular gene of interest overexpressed or knocked out in the epidermis of the skin. We have us作者: 欺騙世家 時(shí)間: 2025-3-24 14:44 作者: Expertise 時(shí)間: 2025-3-24 16:23
Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts,ostnatally upon hormonal stimulation at puberty and full functionality (i.e. lactation) is not achieved until after parturition. Since both the epidermis and mammary glands share the same developmental origin, many mouse models with epidermal phenotypes often also exhibit abnormal mammary gland deve作者: 出血 時(shí)間: 2025-3-24 22:42 作者: NAG 時(shí)間: 2025-3-25 01:42
Kursad TurksenCovers numerous state-of-the-art approaches to analyze epidermal differentiation.Provides step by step reproducible protocols for novices and experienced investigators.Comprises a comprehensive collec作者: Lethargic 時(shí)間: 2025-3-25 06:15 作者: BACLE 時(shí)間: 2025-3-25 10:33
https://doi.org/10.1007/978-1-60761-380-0DNA; Endoplasmatisches Reticulum; Fische; Microarray; Precursors; Progenitors; Proteomics; Vivo; gene expres作者: NOCT 時(shí)間: 2025-3-25 12:55 作者: Asymptomatic 時(shí)間: 2025-3-25 17:35 作者: SPECT 時(shí)間: 2025-3-25 22:58
https://doi.org/10.1007/978-3-642-47558-0se primitive keratinocytes give rise to the progenitors, which are the proliferating keratinocytes and which can be defined as early to late progenitors, according to their differentiation status. Because of the intrinsic heterogeneity of the basal layer, the development of new methods suitable for 作者: Conflict 時(shí)間: 2025-3-26 02:14
Allgemeine und Spezielle ElektrochirurgieIn this perspective, it is important to define minimal culture conditions which allow proper growth and differentiation of epithelial cells in vitro. We propose a simple experimental system which permits the generation of three-dimensional epidermal constructs using a collagen layer as a scaffold mi作者: 開始沒(méi)有 時(shí)間: 2025-3-26 07:52
Martin Kirschner,N. Guleke,O. Kleinschmidtne of defence against environmental physical, chemical and biological agents. The subsequent layer, the dermis, which is mainly formed by fibroblasts, has a more supportive function, containing large amounts of collagen, blood vessels and nerve endings and is less directly affected by external insul作者: monochromatic 時(shí)間: 2025-3-26 11:06
https://doi.org/10.1007/978-3-642-91205-4s. In this case, recovery and cultivation of keratinocytes from these animals provide an opportunity for in vitro studies. Working with isolated keratinocytes is also interesting for certain experiments which cannot be performed in live animals..Primary human keratinocytes can be kept in culture for作者: 遭受 時(shí)間: 2025-3-26 15:12
https://doi.org/10.1007/978-3-642-91566-6tions. These conditions have proven adequate for studies of epidermal differentiation by measurements of the expression of typical early and late differentiation markers. The chapter also deals with the use of quantitative RT-PCR in order to determine the epidermal marker gene expression levels by c作者: 逢迎春日 時(shí)間: 2025-3-26 17:42 作者: Cosmopolitan 時(shí)間: 2025-3-26 21:21 作者: oracle 時(shí)間: 2025-3-27 02:35
https://doi.org/10.1007/978-3-662-00014-4 and differentiation. This chapter describes methods used in our laboratory to maintain HaCaT cells in an undifferentiated state and to use the siRNA technology to efficiently deplete a gene product of interest from these cells. We also provide protocols on how to couple siRNA knockdown with a clona作者: 緯度 時(shí)間: 2025-3-27 06:14
https://doi.org/10.1007/978-3-662-39571-4teins and promise to yield a powerful therapeutic means to dampen the level of proteins that are mutated or frequently overexpressed in skin disease. The efficient and tractable delivery of siRNAs into epidermal keratinocytes is seminal to this process. Here, we describe techniques for transient and作者: 吞吞吐吐 時(shí)間: 2025-3-27 12:23 作者: 執(zhí) 時(shí)間: 2025-3-27 17:33
,Sicherheitsleistung. (232–340.),ation of DNA sequences that interact with transcriptional regulatory proteins is an important step necessary to better understand the molecular mechanisms regulating gene expression. Chromatin immunoprecipitation (ChIP) is one such procedure that provides a snapshot of which transcription factors ar作者: Oratory 時(shí)間: 2025-3-27 20:33
Zeitlicher Geltungsbereich der Rechtsnormen. Here we review the methodology for expression profiling analysis of skin tissue or purified keratinocytes from mice. We explained the methodology and protocols for RNA preservation and purification, RNA quality and integrity tests, and DNA microarray technology types that can be used. Furthermore,作者: BARGE 時(shí)間: 2025-3-27 23:35
Beginn und Ende der natürlichen Persond by its distance to the basal membrane. For that reason Laser Capture Microdissection (LCM) may serve as a perfect tool to compare the characteristics of cells that have been collected from different . of the epithelium. However, as cell boundaries are not visible in untreated tissue sections, samp作者: Ibd810 時(shí)間: 2025-3-28 02:40 作者: Arctic 時(shí)間: 2025-3-28 06:53 作者: Vldl379 時(shí)間: 2025-3-28 12:50 作者: 不能約 時(shí)間: 2025-3-28 14:41
Thermodynamic approach to catalysis,ostnatally upon hormonal stimulation at puberty and full functionality (i.e. lactation) is not achieved until after parturition. Since both the epidermis and mammary glands share the same developmental origin, many mouse models with epidermal phenotypes often also exhibit abnormal mammary gland deve作者: 織物 時(shí)間: 2025-3-28 21:04
Negative Katalyse und Antiklopfmittel,on, formation of epidermal-derived appendages such as hair follicles and formation of the protective barrier. Such analyses have emphasised the heterogenous nature of skin development, perhaps not surprisingly because epidermal development is programmed by heterogenous underlying mesenchyme. It is n作者: HUSH 時(shí)間: 2025-3-29 00:32
https://doi.org/10.1007/978-3-642-91566-6tions. These conditions have proven adequate for studies of epidermal differentiation by measurements of the expression of typical early and late differentiation markers. The chapter also deals with the use of quantitative RT-PCR in order to determine the epidermal marker gene expression levels by comparison with adequate housekeeping genes.作者: 公共汽車 時(shí)間: 2025-3-29 04:12
Amtsrat Wolfgang Te?,Heinz Lohmeyerby in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.作者: 北京人起源 時(shí)間: 2025-3-29 10:12 作者: 適宜 時(shí)間: 2025-3-29 12:59
Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridizationby in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.作者: 引水渠 時(shí)間: 2025-3-29 16:11 作者: hurricane 時(shí)間: 2025-3-29 23:42 作者: harangue 時(shí)間: 2025-3-30 03:39
Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice,ansfection with SV40T or HPV E6E7 genes. This is, however, not suitable if established or hypothetical biochemical links exist between these genes and the pathways or processes to be analysed in the respective experiment..We describe an easy and reproducible method of establishing permanent keratino作者: CURB 時(shí)間: 2025-3-30 07:25 作者: 職業(yè) 時(shí)間: 2025-3-30 10:10
Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes, exact DNA sequence that a transcriptional regulatory protein binds, the precise timing of this association, and what other factors are involved in these interactions are important steps that will shed light on the transcriptional control mechanisms that dictate the biology of all cells, including k作者: 不滿分子 時(shí)間: 2025-3-30 15:06 作者: vitreous-humor 時(shí)間: 2025-3-30 16:37 作者: 大方一點(diǎn) 時(shí)間: 2025-3-31 00:10 作者: 正常 時(shí)間: 2025-3-31 02:30
https://doi.org/10.1007/978-3-642-91205-4ansfection with SV40T or HPV E6E7 genes. This is, however, not suitable if established or hypothetical biochemical links exist between these genes and the pathways or processes to be analysed in the respective experiment..We describe an easy and reproducible method of establishing permanent keratino作者: 油氈 時(shí)間: 2025-3-31 05:19 作者: 附錄 時(shí)間: 2025-3-31 09:32
,Sicherheitsleistung. (232–340.), exact DNA sequence that a transcriptional regulatory protein binds, the precise timing of this association, and what other factors are involved in these interactions are important steps that will shed light on the transcriptional control mechanisms that dictate the biology of all cells, including k作者: 竊喜 時(shí)間: 2025-3-31 16:46 作者: 愛了嗎 時(shí)間: 2025-3-31 21:34 作者: 做方舟 時(shí)間: 2025-4-1 00:24
1064-3745 nregenerative medicine. The protocols follow the successful .Methods in Molecular Biology.? series format, providing introductions to their respective topics, lists of the necessary materials and reagents, and 978-1-61779-663-0978-1-60761-380-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 宏偉 時(shí)間: 2025-4-1 05:47
Book 2010Latest editionasic science research and in regenerative medicine, including would healing and transplantation. .Epidermal Cells: Methods and Protocols, Second Edition. expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step deta作者: 僵硬 時(shí)間: 2025-4-1 06:25
https://doi.org/10.1007/978-3-662-43079-8capacity to terminally differentiate. The use of defined media throughout differentiation allows for detailed characterization of keratinocyte lineage specification from hES cells through the use of gene expression and immunofluorescence analyses.作者: 憤慨一下 時(shí)間: 2025-4-1 12:20 作者: 乳汁 時(shí)間: 2025-4-1 16:52 作者: 吃掉 時(shí)間: 2025-4-1 19:18
Comprehensive Transcriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents, ants, L2L and Venn diagrams for comparing multiple lists of genes, and oPOSSUM for identification of statistically over-represented transcription factor binding sites in the promoter regions of gene sets. The work can be a primer for researchers embarking on skinomics, the comprehensive analysis of transcriptional changes in the skin.作者: syncope 時(shí)間: 2025-4-1 23:19
