標(biāo)題: Titlebook: Epidermal Cells; Methods and Protocol Kursad Turksen Book 20051st edition Humana Press 2005 DNA.PAC.PCR.Promoter.gene expression.genes.repr [打印本頁] 作者: Gram114 時間: 2025-3-21 16:21
書目名稱Epidermal Cells影響因子(影響力)
書目名稱Epidermal Cells影響因子(影響力)學(xué)科排名
書目名稱Epidermal Cells網(wǎng)絡(luò)公開度
書目名稱Epidermal Cells網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Epidermal Cells被引頻次
書目名稱Epidermal Cells被引頻次學(xué)科排名
書目名稱Epidermal Cells年度引用
書目名稱Epidermal Cells年度引用學(xué)科排名
書目名稱Epidermal Cells讀者反饋
書目名稱Epidermal Cells讀者反饋學(xué)科排名
作者: 前兆 時間: 2025-3-21 21:47
Serial Cultivation of Primary Adult Murine Keratinocytescritical first step in developing cellular therapies. Somatic stem cell biology is concerned with stem cells of the adult and how they may be utilized in regenerating tissue and ameliorating disease. Moreover, the incidence of disease increases with age, hence the demand for therapeutics is greatest作者: 現(xiàn)任者 時間: 2025-3-22 01:47
Keratinocyte Culture in the Absence of Substrate Attachment liquid media. This technique has proven to be a powerful tool in studying the effects of loss of extracellular matrix interaction on crucial aspects of epithelial cell biology. Specifically, examining cells in the absence of substrate attachment, as described in this chapter, will allow the investi作者: SLAG 時間: 2025-3-22 07:16
Application of Genetically Modified Feeder Cells for Culture of Keratinocytesd disturbances to such systems are believed to be a critical reason for many diseases, including cancer. Recently, many growth factors mediating epithelial-mesenchymal interactions have been revealed by molecular biological research. These growth factors are critical tools for both basic research an作者: Brain-Imaging 時間: 2025-3-22 09:06 作者: 有毛就脫毛 時間: 2025-3-22 13:47 作者: 有毛就脫毛 時間: 2025-3-22 19:09 作者: 非實(shí)體 時間: 2025-3-23 01:09 作者: 情感 時間: 2025-3-23 04:14
Method for the Harvest and Assay of In Vitro Clonogenic Keratinocytes Stem Cells From Mice washes in provodine iodine and ethanol solutions. The skin of the mice is removed and treated with trypsin to detach the dermis from the epidermis. Cells harvested from this proven method can be used for molecular biology, biochemistry, or a variety of cell culture techniques, including the clonoge作者: 外科醫(yī)生 時間: 2025-3-23 09:03 作者: 機(jī)械 時間: 2025-3-23 13:36
Isolation, Characterization, and Culture of Epithelial Stem Cellsifferentiated, self-renewing cells, which also produce daughter transit amplifying (TA) cells that make up the majority of the proliferative cell population in the tissues. Although still proliferative in nature, it is thought that TA cells can undergo only a finite number of cell divisions before t作者: 債務(wù) 時間: 2025-3-23 16:37 作者: flex336 時間: 2025-3-23 18:44
Epidermal Cell Analysis by RT-PCRzation is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction method to analyze gene expression both in vivo and in vitro.作者: mighty 時間: 2025-3-24 00:03 作者: Ophthalmoscope 時間: 2025-3-24 02:49 作者: Mettle 時間: 2025-3-24 08:53 作者: 管理員 時間: 2025-3-24 13:06 作者: Bumptious 時間: 2025-3-24 15:42
https://doi.org/10.1007/978-3-642-90992-4Immunohistochemistry is an efficient means of localizing specific proteins to their relative expression compartment in the epidermis thereby providing evidence as to their functionality. This chapter therefore describes a dependable method for immunolocalization within the epidermis.作者: 昏迷狀態(tài) 時間: 2025-3-24 20:50
Immunolocalization in the EpidermisImmunohistochemistry is an efficient means of localizing specific proteins to their relative expression compartment in the epidermis thereby providing evidence as to their functionality. This chapter therefore describes a dependable method for immunolocalization within the epidermis.作者: Conquest 時間: 2025-3-25 01:08
https://doi.org/10.1385/1592598307DNA; PAC; PCR; Promoter; gene expression; genes; reproducible techniques作者: Harrowing 時間: 2025-3-25 06:49
Epidermal Cells978-1-59259-830-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 逢迎白雪 時間: 2025-3-25 07:41
Werner Siems,Andreas Bremer,Julia Przyklenkocytes from a single, newborn mouse pup for long-term culture. The protocols we describe will be especially useful for the isolation and analysis of cells harvested from transgenic or knockout mice. We explain how to use a supplemented fibroblast-conditioned medium, along with mouse collagen IV-coat作者: 徹底檢查 時間: 2025-3-25 12:33
https://doi.org/10.1007/978-3-322-93525-0critical first step in developing cellular therapies. Somatic stem cell biology is concerned with stem cells of the adult and how they may be utilized in regenerating tissue and ameliorating disease. Moreover, the incidence of disease increases with age, hence the demand for therapeutics is greatest作者: 尖叫 時間: 2025-3-25 15:49
Die Gewinnung von mechanischer Arbeit, liquid media. This technique has proven to be a powerful tool in studying the effects of loss of extracellular matrix interaction on crucial aspects of epithelial cell biology. Specifically, examining cells in the absence of substrate attachment, as described in this chapter, will allow the investi作者: cavity 時間: 2025-3-25 20:34 作者: adipose-tissue 時間: 2025-3-26 01:55
,Die atmosph?rischen Bewegungsgesetze,anner histologically and temporally similar to the process in vivo. In this culture system, epidermal growth factor specifically and completely inhibited the hair follicle formation with marginal effects on interfollicular epidermis. Using an adenovirus vector, one can transduce a gene into the epid作者: 低三下四之人 時間: 2025-3-26 08:09
https://doi.org/10.1007/978-3-662-29400-0s covered by proliferating and differentiating keratinocytes. Epithelial-mesenchymal interactions are clearly manifest in these skin equivalents. Therefore, they have proven to be suitable experimental tools for a broad range of applications, e.g., for studies on the the paracrine regulation of kera作者: FACET 時間: 2025-3-26 09:26 作者: Latency 時間: 2025-3-26 15:36 作者: Congeal 時間: 2025-3-26 19:57
https://doi.org/10.1007/978-3-642-48202-1 washes in provodine iodine and ethanol solutions. The skin of the mice is removed and treated with trypsin to detach the dermis from the epidermis. Cells harvested from this proven method can be used for molecular biology, biochemistry, or a variety of cell culture techniques, including the clonoge作者: grounded 時間: 2025-3-26 22:46 作者: Motilin 時間: 2025-3-27 01:33
Giftallergie (Idiosynkrasie gegen Gifte),ifferentiated, self-renewing cells, which also produce daughter transit amplifying (TA) cells that make up the majority of the proliferative cell population in the tissues. Although still proliferative in nature, it is thought that TA cells can undergo only a finite number of cell divisions before t作者: Genome 時間: 2025-3-27 05:51 作者: Cumulus 時間: 2025-3-27 13:09
,Die Zweckm??igkeitslehre oder Teleologie,zation is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction method to analyze gene expression both in vivo and in vitro.作者: 責(zé)難 時間: 2025-3-27 13:54
Allgemeine Eigenschaften der Protoplasten,n, formation of epidermal-derived appendages such as hair follicles, and formation of the protective barrier. Such analyses have emphasized the heterogenous nature of skin development, perhaps not surprising because epidermal development is programmed by heterogenous underlying mesenchyme. It is nec作者: 投射 時間: 2025-3-27 21:32
https://doi.org/10.1007/978-3-642-47418-7divergence, there are important similarities between the epidermis of fish and mammals. In addition, recent work suggests that the genes required for epidermal development in the zebrafish also are essential for this process in higher vertebrates, indicating that analysis of the zebrafish system wil作者: Ancillary 時間: 2025-3-28 00:44
https://doi.org/10.1007/978-3-642-48202-1 washes in provodine iodine and ethanol solutions. The skin of the mice is removed and treated with trypsin to detach the dermis from the epidermis. Cells harvested from this proven method can be used for molecular biology, biochemistry, or a variety of cell culture techniques, including the clonogenic cell assay presented here.作者: 急急忙忙 時間: 2025-3-28 05:30
,Die Zweckm??igkeitslehre oder Teleologie,zation is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction method to analyze gene expression both in vivo and in vitro.作者: 入會 時間: 2025-3-28 09:20 作者: Fracture 時間: 2025-3-28 13:43
Epidermal Cell Analysis by RT-PCRzation is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction method to analyze gene expression both in vivo and in vitro.作者: ELATE 時間: 2025-3-28 16:58 作者: MURAL 時間: 2025-3-28 18:53
https://doi.org/10.1007/978-3-662-41090-5their expression of keratin 19 will be described in detail. The methods include the double labeling of tissues for keratin 19 and label-retaining cells (autoradiography of .H-thymidine) .. The labeling of keratin 19 by immunofluorescence of by flow cytometry is described for cells in vitro.作者: Frequency-Range 時間: 2025-3-28 23:18
Isolation, Characterization, and Culture of Epithelial Stem Cellsoped the method described here to isolate this population of epidermal stem cells (EpiSC). We showed that EpiSC are all keratin 14. and thus of keratinocyte origin and not of mesenchymal or hematopoietic origin. We have also demonstrated that EpiSC can regenerate the epidermis, that they can permane作者: 基因組 時間: 2025-3-29 05:53 作者: blackout 時間: 2025-3-29 07:22 作者: CRANK 時間: 2025-3-29 14:02
1064-3745 ible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.978-1-59259-830-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: abysmal 時間: 2025-3-29 15:58 作者: 鄙視 時間: 2025-3-29 21:26 作者: 重力 時間: 2025-3-30 02:12
Allgemeine Eigenschaften der Protoplasten,ormation of the protective barrier is the endpoint of epidermal terminal differentiation and defects in this process are reflected in failure, acceleration, or delay in barrier formation. Hence, these latter assays are of particular value as a rapid initial assay for epidermal developmental defects in genetically modified organisms.作者: 祖?zhèn)髫敭a(chǎn) 時間: 2025-3-30 06:14
https://doi.org/10.1007/978-3-642-47418-7The aim of this chapter is to provide the researcher with basic protocols to examine gene expression, cell proliferation, and cell death in the developing zebrafish. Although only a few specific epidermal markers have been identified in the zebrafish thus far, we also provide a list of useful genes for epidermal analysis.作者: 音樂等 時間: 2025-3-30 08:22 作者: allude 時間: 2025-3-30 12:33
Serial Cultivation of Primary Adult Murine Keratinocytesand serially culture adult basal keratinocytes from the epidermis of the mouse in a supplemented culture medium that selects for epithelial lineages and enhances their proliferation while inhibiting differentiation through many passages.作者: 一再困擾 時間: 2025-3-30 18:18 作者: 庇護(hù) 時間: 2025-3-30 20:57
Analysis of Early Epidermal Development in ZebrafishThe aim of this chapter is to provide the researcher with basic protocols to examine gene expression, cell proliferation, and cell death in the developing zebrafish. Although only a few specific epidermal markers have been identified in the zebrafish thus far, we also provide a list of useful genes for epidermal analysis.作者: Musculoskeletal 時間: 2025-3-31 03:06 作者: Brain-Imaging 時間: 2025-3-31 08:15 作者: 上漲 時間: 2025-3-31 10:56
In Vivo Labeling and Analysis of Epidermal Stem Cellsulsion and allowed to dry in light tight box for 4 wk at 4°C. After fixation with photographic fixer, the slides were washed, lightly stained, and viewed with a light microscope to observe and quantify cells that retained the labeling. Label-retaining cells were identified by the black silver grains over their nuclei.作者: 討好美人 時間: 2025-3-31 16:01
Book 20051st editionpidermal cells and their characterization, and applications in regenerative medicine.These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.
