標(biāo)題: Titlebook: Epiblast Stem Cells; Methods and Protocol Pierre Osteil Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license [打印本頁(yè)] 作者: Iridescent 時(shí)間: 2025-3-21 17:41
書目名稱Epiblast Stem Cells影響因子(影響力)
作者: 豐滿中國(guó) 時(shí)間: 2025-3-21 23:27 作者: 吹牛者 時(shí)間: 2025-3-22 02:50
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/313140.jpg作者: white-matter 時(shí)間: 2025-3-22 05:10
978-1-0716-2283-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Flatus 時(shí)間: 2025-3-22 12:41
Epiblast Stem Cells978-1-0716-2281-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 千篇一律 時(shí)間: 2025-3-22 16:32 作者: 千篇一律 時(shí)間: 2025-3-22 19:58 作者: 吸引力 時(shí)間: 2025-3-23 00:45
Air Pollution Effects on Biodiversityotency is a continuum of different states between the two extremes of na?ve embryonic stem cells (ESCs) and primed Epiblast Stem Cells (EpiSCs), which resemble the pre/peri-?and post- implantation embryo, respectively. The transition from na?ve to primed pluripotency can be induced by growing na?ve 作者: 憤慨點(diǎn)吧 時(shí)間: 2025-3-23 04:20
https://doi.org/10.1007/978-1-4757-4465-1 development of a mouse embryo allows us to identify the genetic basis of errors of development in animal disease models. Immunofluorescence is a powerful technique to study the localization and variation in expression pattern of specific proteins in cells, tissues, and organs. Detecting the antigen作者: 健談 時(shí)間: 2025-3-23 06:03
Perry J. Samson,Jennie L. Moodyue to innate differences in cellular characteristics, the efficiency of lipid-based transfection is variable across cell types. Pluripotent cells which exist in a “primed” state such as human embryonic stem cells (hESCs) and mouse epiblast stem cells (mEpiSCs) are notorious for being refractory to l作者: 確保 時(shí)間: 2025-3-23 12:03 作者: 設(shè)施 時(shí)間: 2025-3-23 17:09
PPI-Theory for Particle Dispersion microscopy analysis. Colocalization assessment of nuclear and cytoplasmic cell regions is detailed to demonstrate nuclear and cytoplasmic localization in mEpiSCs by confocal microscopy and orthogonal colocalization assessment. Protein colocalization within mESCs, mEpiLCs, and mEpiSCs can be efficie作者: mechanical 時(shí)間: 2025-3-23 19:04 作者: pantomime 時(shí)間: 2025-3-23 23:39
Air Quality Modeling in Complex Terrainsify putative regulatory regions, determine dynamics and mechanisms of transcription factors when coupled with ChIP-seq and predict interactions between proteins and chromatin. Compared to previous methods, MNase-seq and DNase-seq, ATAC-seq requires only 50,000 cells, orders of magnitude fewer cells.作者: Excitotoxin 時(shí)間: 2025-3-24 04:29
W. E. Davis,A. R. Olsen,B. T. Didierlk populations. However most of the methods focus on the 3′-end of polyadenylated transcripts using droplet-based technology. To achieve complete transcriptome, we describe single-cell 5′-end transcriptome protocol with random primed-cDNA harvesting on the Fluidigm C1? platform which can isolate and作者: anaerobic 時(shí)間: 2025-3-24 08:17 作者: collagen 時(shí)間: 2025-3-24 13:28 作者: Fatten 時(shí)間: 2025-3-24 18:18
P. Grossi,G. Graziani,C. Ceruttills (ESCs) aggregated in suspension culture are able to form 3D embryo-like structures called gastruloids that mimic features of the gastrulation process. Recent findings indicate that gastruloid formation efficiency decreases as pluripotency progresses from na?ve to primed state, and suggest that g作者: 蘑菇 時(shí)間: 2025-3-24 20:23
G. Kallos,V. Kotroni,K. Lagouvardosmbryos. The basis of this protocol mimicking the in vivo gastrulation process makes a contrast with those using sequential administration of pharmacological molecules and recombinant signaling proteins even at nonphysiological levels. In the experimental setup, EpiSCs are first freed from the dish-a作者: Asperity 時(shí)間: 2025-3-24 23:19 作者: 外露 時(shí)間: 2025-3-25 06:32
M. van Loon,P. J. H. Builtjes,A. J. SegersThe ability to generate primordial germ cell-like cells (PGCLCs) from murine embryonic stem cells (ESCs) has enabled in vitro investigation of the molecular mechanisms regulating this process without the use of a mouse model. Here we describe the procedures from the culture of ESCs to the detection of PGCLCs in the embryoid bodies (spheroids).作者: 啞劇 時(shí)間: 2025-3-25 11:00
A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq DataSingle-cell RNA-sequencing (scRNA-Seq) is a widely used technology to reveal the heterogeneity and dynamics of tissues, organisms, and complex diseases. Here, a workflow is presented for preprocessing of scRNA-seq data to quantify gene abundances in individual cells followed by visualization and annotation of cells.作者: intrigue 時(shí)間: 2025-3-25 13:57
In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-like Cells The ability to generate primordial germ cell-like cells (PGCLCs) from murine embryonic stem cells (ESCs) has enabled in vitro investigation of the molecular mechanisms regulating this process without the use of a mouse model. Here we describe the procedures from the culture of ESCs to the detection of PGCLCs in the embryoid bodies (spheroids).作者: Ingrained 時(shí)間: 2025-3-25 19:29
Heinz Brauer,Yalamanchili B. G. Varmat stem cells as they share similar properties, such as their incapability to contribute to the formation of an embryo after injection into blastocyst. The epiblast stem cells (EpiSC) have delineated a novel status of pluripotency called “primed.” How to establish EpiSC from mouse embryo is described in detail in this chapter.作者: Notorious 時(shí)間: 2025-3-25 23:55
PPI-Theory for Particle Dispersion microscopy analysis. Colocalization assessment of nuclear and cytoplasmic cell regions is detailed to demonstrate nuclear and cytoplasmic localization in mEpiSCs by confocal microscopy and orthogonal colocalization assessment. Protein colocalization within mESCs, mEpiLCs, and mEpiSCs can be efficiently completed using these optimized protocols.作者: 胎兒 時(shí)間: 2025-3-26 03:56
Establishment of Mouse Epiblast Stem Cellst stem cells as they share similar properties, such as their incapability to contribute to the formation of an embryo after injection into blastocyst. The epiblast stem cells (EpiSC) have delineated a novel status of pluripotency called “primed.” How to establish EpiSC from mouse embryo is described in detail in this chapter.作者: osculate 時(shí)間: 2025-3-26 06:21
3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells microscopy analysis. Colocalization assessment of nuclear and cytoplasmic cell regions is detailed to demonstrate nuclear and cytoplasmic localization in mEpiSCs by confocal microscopy and orthogonal colocalization assessment. Protein colocalization within mESCs, mEpiLCs, and mEpiSCs can be efficiently completed using these optimized protocols.作者: 同步左右 時(shí)間: 2025-3-26 08:31 作者: fibroblast 時(shí)間: 2025-3-26 14:21
Air Quality Modeling in Complex Terrainsn proteins and chromatin. Compared to previous methods, MNase-seq and DNase-seq, ATAC-seq requires only 50,000 cells, orders of magnitude fewer cells. In addition, the ATAC-seq protocol takes one day to progress from cells to sequencing ready libraries.作者: 歪曲道理 時(shí)間: 2025-3-26 19:46
Jutta Graf,Hans Schlager,Monika Krautstrunkrdinated to allow proper organismal development. Here, using mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) as model systems, we describe a robust protocol that allows comprehensive and comparative structural analysis of the glycome.作者: 邊緣 時(shí)間: 2025-3-27 00:21
Generation of Epiblast-Like CellsESCs in EpiSCs medium, containing bFGF and Activin. Here we report the detailed protocol to generate and characterize the epiblast-like cells (EpiLCs), which correspond to a primed intermediate state between na?ve ESCs and EpiSCs.作者: 阻擋 時(shí)間: 2025-3-27 04:14 作者: 特別容易碎 時(shí)間: 2025-3-27 07:08 作者: anthropologist 時(shí)間: 2025-3-27 11:04 作者: 頑固 時(shí)間: 2025-3-27 15:05 作者: 裝勇敢地做 時(shí)間: 2025-3-27 20:29 作者: 閑聊 時(shí)間: 2025-3-27 23:59
G. Kallos,V. Kotroni,K. Lagouvardos the endoderm precursors to interact with the Matrigel mimicking the laminin-rich basement membrane underlying the egg cylinder epiblast in embryos, and let the precursors migrate into the Matrigel-filled external zone and develop into endodermal epithelial tissues.作者: 蛙鳴聲 時(shí)間: 2025-3-28 03:25
Definitive Endoderm from EpiSC Aggregates in Matrigel the endoderm precursors to interact with the Matrigel mimicking the laminin-rich basement membrane underlying the egg cylinder epiblast in embryos, and let the precursors migrate into the Matrigel-filled external zone and develop into endodermal epithelial tissues.作者: 朦朧 時(shí)間: 2025-3-28 09:30 作者: 描述 時(shí)間: 2025-3-28 12:42 作者: 無(wú)能性 時(shí)間: 2025-3-28 14:57
Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cellsrdinated to allow proper organismal development. Here, using mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) as model systems, we describe a robust protocol that allows comprehensive and comparative structural analysis of the glycome.作者: insolence 時(shí)間: 2025-3-28 20:31 作者: FATAL 時(shí)間: 2025-3-29 02:45 作者: 對(duì)手 時(shí)間: 2025-3-29 06:34
Perry J. Samson,Jennie L. Moodyipid-based transfection systems. Herein we describe a forward transfection protocol which we routinely use to achieve upwards of 70% transfection efficiency rates in mEpiSCs. Our protocol also includes a suggested transfection timeline and details pertaining to the techniques we use to validate transfection success.作者: 雄偉 時(shí)間: 2025-3-29 08:42 作者: 動(dòng)機(jī) 時(shí)間: 2025-3-29 15:27
W. E. Davis,A. R. Olsen,B. T. Didier process up to 96 cells from a single run with custom library preparation. The method enables detection of Transcription Start Site (TSS) at the single-cell resolution yielding a more comprehensive overview of gene regulatory elements governing in the EpiSC-like cell (EpiLC) including non-polyadenylated RNA and enhancer RNA activities.作者: 白楊 時(shí)間: 2025-3-29 18:35 作者: 暫時(shí)休息 時(shí)間: 2025-3-29 23:20
Volatile Organic Compounds (VOCs) Control,persed colonies, have an increased proliferation rate, and a predisposition to neural fate. EPL cells can be used to study the cell states along the pluripotency continuum, peri-implantation embryogenesis, and as a starting point for efficient neuronal differentiation.作者: Devastate 時(shí)間: 2025-3-30 03:14 作者: Glaci冰 時(shí)間: 2025-3-30 06:14
L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Plurpersed colonies, have an increased proliferation rate, and a predisposition to neural fate. EPL cells can be used to study the cell states along the pluripotency continuum, peri-implantation embryogenesis, and as a starting point for efficient neuronal differentiation.作者: overweight 時(shí)間: 2025-3-30 10:41 作者: Gustatory 時(shí)間: 2025-3-30 12:53 作者: 類似思想 時(shí)間: 2025-3-30 17:08 作者: Brocas-Area 時(shí)間: 2025-3-30 23:26 作者: 直覺(jué)沒(méi)有 時(shí)間: 2025-3-31 02:09 作者: transplantation 時(shí)間: 2025-3-31 07:57
Establishment of Mouse Epiblast Stem Cellst stem cells as they share similar properties, such as their incapability to contribute to the formation of an embryo after injection into blastocyst. The epiblast stem cells (EpiSC) have delineated a novel status of pluripotency called “primed.” How to establish EpiSC from mouse embryo is described作者: 美學(xué) 時(shí)間: 2025-3-31 10:36
L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Plurg from the least mature “ground state” to being “primed” to differentiate. Cells along this continuum are demarcated by differences in gene expression, X chromosome inactivation, ability to form chimeras and epigenetic marks. We have developed a protocol to differentiate “na?ve” mESCs to a “partiall作者: 脫離 時(shí)間: 2025-3-31 15:18 作者: 凝視 時(shí)間: 2025-3-31 19:57
Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence development of a mouse embryo allows us to identify the genetic basis of errors of development in animal disease models. Immunofluorescence is a powerful technique to study the localization and variation in expression pattern of specific proteins in cells, tissues, and organs. Detecting the antigen作者: 朝圣者 時(shí)間: 2025-3-31 22:35
Small Interfering RNA (siRNA) Transfection in Epiblast Stem Cellsue to innate differences in cellular characteristics, the efficiency of lipid-based transfection is variable across cell types. Pluripotent cells which exist in a “primed” state such as human embryonic stem cells (hESCs) and mouse epiblast stem cells (mEpiSCs) are notorious for being refractory to l
