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標(biāo)題: Titlebook: Enzymes, Receptors, and Carriers of Biological Membranes; A Laboratory Manual Angelo Azzi,Urs Brodbeck,Peter Zahler Book 1984 Springer-Verl [打印本頁]

作者: sesamoiditis    時(shí)間: 2025-3-21 16:59
書目名稱Enzymes, Receptors, and Carriers of Biological Membranes影響因子(影響力)




書目名稱Enzymes, Receptors, and Carriers of Biological Membranes影響因子(影響力)學(xué)科排名




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書目名稱Enzymes, Receptors, and Carriers of Biological Membranes網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Enzymes, Receptors, and Carriers of Biological Membranes被引頻次




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書目名稱Enzymes, Receptors, and Carriers of Biological Membranes年度引用學(xué)科排名




書目名稱Enzymes, Receptors, and Carriers of Biological Membranes讀者反饋




書目名稱Enzymes, Receptors, and Carriers of Biological Membranes讀者反饋學(xué)科排名





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Simon Brugger,Rebecca Haubenreisser work was based on the previously developed technique for the transfer of nucleic acids onto papers or membranes of derivatized cellulose and, together with the technical know-how of several manufacturers, opened a wide new area of experimental procedures in protein analysis (for a review see . of B
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,Durchführung agiles Innovationsmanagement,ed by their solubilization and sedimentation behavior. One class comprises the high salt-soluble enzyme forms (HSS-AChE), the other the detergent-soluble enzyme (DS-AChE). Both classes can be purified by affinity chromatography. HSS-AChE is an asymmetric collagen tailed form sedimenting at 17S and 1
作者: 引水渠    時(shí)間: 2025-3-22 11:25
https://doi.org/10.1007/978-3-658-42577-7for characterization of these hydrophobic components: These include hydrophobic labelling, sequence determination, hydrophobic chromatography and phase partition. An elegant version of the latter technique was described by Bordier (1981). This consists of using a non-ionic detergent with a cloud-poi
作者: 污點(diǎn)    時(shí)間: 2025-3-22 14:27
https://doi.org/10.1007/978-3-658-22706-7s and their fatty acid composition. The scope of the experiment is the separation of a lipid mixture by two-dimensional TLC and the subsequent separation and quantitative determination by GLC of the fatty acids. From the total amount of fatty acids, the amount of particular lipid components is calcu
作者: 污點(diǎn)    時(shí)間: 2025-3-22 20:22
Agiles Marketing Performance Managementter layer consists mainly of choline-containing phospholipids, whereas the aminophospholipids are found preferentially in the inner layer. Phospholipid asymmetry is furthermore detectable in plasma membranes of eukaryotic cells, in virus membranes, and in various bacterial membrane systems.
作者: 勾引    時(shí)間: 2025-3-22 21:24
Agiles Prozessmanagement im Krankenhauss X-ray crystallography. Crystallography depends on the availability of large, well-ordered crystals. Membrane proteins could not be crystallized until recently. The main reason for the lack of success in the crystallization of membrane proteins lies in the amphiphilic nature of the membrane protein
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作者: 畸形    時(shí)間: 2025-3-23 08:22
https://doi.org/10.1007/978-3-658-31001-1ATP, a key process in the cellular energy supply of aerobic organism (for review see Klingenberg 1980; Vignais 1976; Vignais et al. 1982). In beef heart mitochondria the translocator is the most abundant integral protein, being about 10% of the total mitochondrial protein. The M. of the monomeric tr
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Oedipus and the Meaning of Agingxygen, and as a proton pump, thus participating as a redox pump in the conversion of metabolic energy during the synthesis of ATP (Azzi 1980; Wainio 1983). Cytochrome oxidase has been purified from mitochondria of different sources. The most common technique used to purify beef heart cytochrome oxid
作者: 苦惱    時(shí)間: 2025-3-24 01:46
Molecular Basis of Androgenetic Alopecia,s of that intrinsic to the proteins have provided a considerable insight into the structure and mobility of these systems. The use of fluorescent probes to study membrane proteins is illustrated in the practical exercise described here, where the interaction of the mercurial reagent fluorescein merc
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作者: 大量殺死    時(shí)間: 2025-3-24 12:17

作者: 分散    時(shí)間: 2025-3-24 16:34
Reconstitution of Na, K-ATPaseThe Na, K-ATPase (EC 3.6.1.2.) or sodium pump is a membrane-spanning protein-lipid complex composed of a catalytic α subunit (about 100,000 mol wt), of a β subunit (35–65,000 mol wt) and of phospholipid and cholesterol (for a recent review, see J?rgensen 1982).
作者: Aphorism    時(shí)間: 2025-3-24 21:04
978-3-540-13751-1Springer-Verlag Berlin Heidelberg 1984
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Localization of Phosphatidylglycerol in the Membrane of Acholeplasma laidlawiiter layer consists mainly of choline-containing phospholipids, whereas the aminophospholipids are found preferentially in the inner layer. Phospholipid asymmetry is furthermore detectable in plasma membranes of eukaryotic cells, in virus membranes, and in various bacterial membrane systems.
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Investigation of Amphiphilic Nature of Different Forms of Acetylcholinesterase from Torpedo Marmoratre attached to collagen-like tails forming a triple helix. DS-AChE is a dimer with a S-value of 6.5S in the presence of Triton X-100 and has a subunit molecular weight of 66,000. By limited digestion of DS-AChE with proteinase K a catalytically active enzyme sedimenting at 7.5S is formed.
作者: Simulate    時(shí)間: 2025-3-27 03:17
Separation of Hydrophobic Membrane Proteins by Phase Partition: Characterization by Two-Dimensional e partition. An elegant version of the latter technique was described by Bordier (1981). This consists of using a non-ionic detergent with a cloud-point somewhat higher than room temperature to partition proteins into hydrophobic and hydrophilic fractions.
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Selective Labeling of the ADP/ATP Translocator with Eosin-5-Maleimidecontains 4 cysteins over 297 amino acids. Masking or modification of sulfydryl groups, which are essential for the nucleotide translocation, causes the inhibition of the translocation activity. At least one sulfydryl group is maked when CAT is bound to the translocator.
作者: 使痛苦    時(shí)間: 2025-3-28 12:30
Site-Directed Hydrophobic Labeling of Membrane Proteins: NBD-Modification of Bacteriorhodopsinintramembraneous disposition of a protein arises from hydrophobic labeling studies. Those segments of intrinsic membrane proteins that are in close association with the hydrocarbon core of a lipid bilayer may be identified by using hydrophobic photogenerated reagents (Gitler and Bercovici 1980, Brunner and Semenza 1981, Ross et al. 1982).
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Electrophoretic Transfer of Chloroplast Membrane Proteins from SDS-Gels onto Nitrocellulose and Thei work was based on the previously developed technique for the transfer of nucleic acids onto papers or membranes of derivatized cellulose and, together with the technical know-how of several manufacturers, opened a wide new area of experimental procedures in protein analysis (for a review see . of B
作者: Axon895    時(shí)間: 2025-3-29 00:09
Investigation of Amphiphilic Nature of Different Forms of Acetylcholinesterase from Torpedo Marmorated by their solubilization and sedimentation behavior. One class comprises the high salt-soluble enzyme forms (HSS-AChE), the other the detergent-soluble enzyme (DS-AChE). Both classes can be purified by affinity chromatography. HSS-AChE is an asymmetric collagen tailed form sedimenting at 17S and 1
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Site-Directed Hydrophobic Labeling of Membrane Proteins: NBD-Modification of Bacteriorhodopsinembranes are presumed to span the lipid bilayer. The proteins’ transmembrane disposition can be assayed by various techniques including labeling procedures with hydrophilic chemical reagents, enzyme-mediated modifications, protease accessibility and antibody interaction. Convincing evidence for the
作者: 癡呆    時(shí)間: 2025-3-30 11:17
Import of Proteins by Isolated Mitochondria bulk of mitochondrial protein is encoded on nuclear genes. Nuclear gene products are synthesized in the extramitochondrial cytoplasm and then incorporated into mitochondria by a process called import. This process consists of a few distinct steps: translation of the proteins as precursors, recognit
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