標題: Titlebook: Enzymes of Molecular Biology; Michael M. Burrell Book 1993 Humana Press 1993 [打印本頁] 作者: Corticosteroids 時間: 2025-3-21 18:52
書目名稱Enzymes of Molecular Biology影響因子(影響力)
書目名稱Enzymes of Molecular Biology影響因子(影響力)學科排名
書目名稱Enzymes of Molecular Biology網(wǎng)絡(luò)公開度
書目名稱Enzymes of Molecular Biology網(wǎng)絡(luò)公開度學科排名
書目名稱Enzymes of Molecular Biology被引頻次
書目名稱Enzymes of Molecular Biology被引頻次學科排名
書目名稱Enzymes of Molecular Biology年度引用
書目名稱Enzymes of Molecular Biology年度引用學科排名
書目名稱Enzymes of Molecular Biology讀者反饋
書目名稱Enzymes of Molecular Biology讀者反饋學科排名
作者: CANDY 時間: 2025-3-21 20:37
1064-3745 older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzy978-0-89603-234-7978-1-59259-503-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Mucosa 時間: 2025-3-22 03:21 作者: 采納 時間: 2025-3-22 05:32 作者: anthropologist 時間: 2025-3-22 12:47 作者: 憎惡 時間: 2025-3-22 15:14 作者: 憎惡 時間: 2025-3-22 19:48 作者: acclimate 時間: 2025-3-23 00:54
Aminopeptidases,peptides and proteins are genuine posttranslational modifications, or were introduced by cyclization of N-terminal glutamine during purification) or during sequence determination when glutamine was the newly liberated N-terminal amino acid. This cyclized derivative does not have a free amino group, 作者: famine 時間: 2025-3-23 04:11
https://doi.org/10.1007/978-3-662-60378-9ial can be gaged however by considering that several enzymes implicated in DNA replication, recombination, and repair have integral exo- or endodeoxy-ribonuclease activity. For example, the 5′-3′ and 3′–5′ exonuclease activity of DNA polymerases and the endo-DNase activity of topoisomerases (e.g., .作者: CONE 時間: 2025-3-23 06:19
Vorstellung der Fallstudie ?KnowBeer“on, parameters for carrying out first-strand cDNA synthesis from poly(A). mRNA populations with AMV RT have been optimized thoroughly (.); standard optima exist for pH, nucleotide concentration, and monovalent and divalent cation concentration; also essential stimulatory additives (such as sodium py作者: Cholagogue 時間: 2025-3-23 23:28 作者: lacrimal-gland 時間: 2025-3-24 02:33 作者: micturition 時間: 2025-3-24 08:57 作者: Dealing 時間: 2025-3-24 14:14 作者: 類型 時間: 2025-3-24 15:12
https://doi.org/10.1007/978-1-4842-2102-0ed), reverse transcriptases (EC 2.7.7.49, RNA-dependent DNA polymerases that utilize an RNA template), and terminal deoxynucleotidyl transferases (EC 2.7.7.31, which require no template). This chapter will consider only DNA-dependent DNA polymerases (EC 2.7.7.7, DNA nucleotidyltransferases, DNA-directed).作者: Flatus 時間: 2025-3-24 21:26
https://doi.org/10.1007/978-3-662-57305-1e N. or 5-C position of dC (EC 2.1.1.73) depending on the particular enzyme (.). The reaction is predominantly irreversible. Enzymes, such as ..-methylguanine DNA Mtase, that participate in DNA repair processes will not be discussed here.作者: instulate 時間: 2025-3-24 23:52
https://doi.org/10.1007/978-3-658-42460-2 located in many organs, including bone marrow, kidney, placenta, and intestinal mucosa), but have not been isolated from higher plants. The most commonly used alkaline phosphatases (AP) are those from calf intestinal mucosa (called CIAP, CIP, or CAP) and from the bacterium . (BAP).作者: STALL 時間: 2025-3-25 05:10
The BAL 31 Nucleases (EC 3.1.11),e shown (.,.) to be bacterial products. Only 10–20% of the nuclease activity is found in the periplasm (.). The American Type Culture Collection strain of . (ATCC 29659) produces BAL 31 nuclease as proficiently as the strain originally obtained from its discoverer.作者: 血友病 時間: 2025-3-25 09:14 作者: BLOT 時間: 2025-3-25 13:24
Pronase (EC 3.4.24.4),used, for example, to reveal the protein components of cell organelles by the hydrolysis of tissue slices (.), and as an alternative to proteinase K to remove protein during plasmid DNA (.), chromosomal DNA (.), and RNA isolation (.–.). Another use of pronase is the production of a protein hydrolysate suitable for amino acid analysis (.,.).作者: avarice 時間: 2025-3-25 19:32
Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1),lar biology, The Type II restriction endonucleases obviously fall into this category, however they are a large subject on their own and therefore are dealt with in a separate section of this book. DNases acting preferentially on single-stranded DNA (ss DNA) substrates are also dealt with in another section of this chapter.作者: 不可接觸 時間: 2025-3-25 23:27 作者: 惰性女人 時間: 2025-3-26 03:27 作者: COW 時間: 2025-3-26 06:27 作者: 亞麻制品 時間: 2025-3-26 11:53 作者: cumulative 時間: 2025-3-26 16:06
Outlook: Agile as the New Normal?,homononucleotides. This chapter focuses on the endoribonuclease RNase A (otherwise described as RNase, RNase I, or pancreatic ribonuclease), which shows some base specificity in where it cleaves RNA. The enzyme has been particularly well characterized at the molecular level.作者: 雄辯 時間: 2025-3-26 17:41
Agile in der Unternehmenspraxisused, for example, to reveal the protein components of cell organelles by the hydrolysis of tissue slices (.), and as an alternative to proteinase K to remove protein during plasmid DNA (.), chromosomal DNA (.), and RNA isolation (.–.). Another use of pronase is the production of a protein hydrolysate suitable for amino acid analysis (.,.).作者: fatty-acids 時間: 2025-3-27 00:14
Agile eHealth Usability Evaluationely degrade double-stranded DNA (.–.). Mung-bean nuclease 1 is also reported to show a separate 3′-ω-monophosphatase activity (.) (..). Mung-bean nuclease 1 is a zinc metalloenzyme that requires Zn. and a reducing agent, such as cysteine, for both activity and stability.作者: 現(xiàn)實 時間: 2025-3-27 01:27
Mung-Bean Nuclease 1 (EC 3.1.30.1),ely degrade double-stranded DNA (.–.). Mung-bean nuclease 1 is also reported to show a separate 3′-ω-monophosphatase activity (.) (..). Mung-bean nuclease 1 is a zinc metalloenzyme that requires Zn. and a reducing agent, such as cysteine, for both activity and stability.作者: 不妥協(xié) 時間: 2025-3-27 05:16 作者: 無意 時間: 2025-3-27 10:43 作者: 松軟 時間: 2025-3-27 16:19
https://doi.org/10.1007/978-3-662-60378-9al enzymes that cleave DNA molecules in a base-specific manner. These enzymes, the type II restriction endonucleases, are perhaps the best studied of the nucleases as to both their in vivo role and their use as tools in the techniques of molecular biology. However, the nucleases are ubiquitous in li作者: ineffectual 時間: 2025-3-27 18:48
Agile Strategy Management in the Digital Ageses that preferentially catalyze the hydrolysis of double-stranded DNA (ds DNA) and that have found a use in the various techniques employed in molecular biology, The Type II restriction endonucleases obviously fall into this category, however they are a large subject on their own and therefore are 作者: 情感脆弱 時間: 2025-3-28 00:46
https://doi.org/10.1007/978-1-4842-2102-0lassed as DNA-dependent DNA polymerases (EC 2.7.7.7, which require both a DNA template strand and a DNA primer to which the monomeric units can be added), reverse transcriptases (EC 2.7.7.49, RNA-dependent DNA polymerases that utilize an RNA template), and terminal deoxynucleotidyl transferases (EC 作者: 眨眼 時間: 2025-3-28 03:04 作者: 打谷工具 時間: 2025-3-28 06:56
Vorstellung der Fallstudie ?KnowBeer“ first in this process, retroviral RNA-directed DNA polymerase (reverse transcriptase), catalyzes the synthesis of a DNA copy of an RNA template in the presence of a suitable primer. Reverse transcriptase (RT) was discovered in 1970 (.,.) and was first used to copy a eukaryotic cell mRNA in 1971 (.–作者: carotid-bruit 時間: 2025-3-28 10:54
Skalierung – teamübergreifende Abstimmungynucleotide primers. Different from all the other known DNA polymerases, TdT-catalyzed DNA synthesis is not template-directed. The reaction can be described as: . Only one dNTP is necessary for polymerization, although any dNTP (and derivatives thereof) or any combination of dNTPs will serve as subs作者: 不持續(xù)就爆 時間: 2025-3-28 16:33 作者: 縱火 時間: 2025-3-28 20:29
https://doi.org/10.1007/978-3-662-57305-1ases within defined DNA sequences (.). Individual enzymes are specific for one or the other base, and modify at the 6-NH. of dA (EC 2.1.1.72) or at the N. or 5-C position of dC (EC 2.1.1.73) depending on the particular enzyme (.). The reaction is predominantly irreversible. Enzymes, such as ..-methy作者: PLUMP 時間: 2025-3-29 02:14 作者: 商談 時間: 2025-3-29 03:16 作者: FISC 時間: 2025-3-29 09:24
Outlook: Agile as the New Normal?,f RNA. Exonucleases may cleave the RNA in 3′-5′ direction or vice versa, whereas some endoribonucleases have a specific requirement for certain bases. For example the RNase from . cleaves at pyrimidine residues (.). Some enzymes produce 5′ phosphomononucleotides, whereas others give rise to 3′ phosp作者: Robust 時間: 2025-3-29 14:28
Agile in der Unternehmenspraxist least ten proteolytic components: five serine-type proteases, two Zn. endopeptidases, two Zn.-leucine aminopeptidases, and one Zn. carboxypeptidase (.,.). Pronase therefore has very broad specificity, and is used in cases where extensive or complete degradation of protein is required. It has been 作者: 愛好 時間: 2025-3-29 17:25
The enemy: Big Upfront Anything, by . proteolysis, a large number of relatively small (5–20 residues) peptides either for peptide mapping (. vol. 1, .) or for purification and subsequent manual sequence determination by the dansyl-Edman method (. vol. 1, Chapter 24). The second reason is to generate relatively large peptides (50–1作者: 龍蝦 時間: 2025-3-29 21:49
Testing-Based Formal Verification,tide bonds C-terminal to a number of amino acids. The enzyme is produced, together with other proteases and an aminopeptidase, during stationary phase when the fungus is grown by submerged culture. The enzyme is so named because the organism can grow on native keratin as sole carbohydrate and nitrog作者: CANON 時間: 2025-3-30 00:46
Agiles Projektmanagement im Einkauf,ey are exoproteases. A number of such enzymes have been isolated from plant and animal sources, each differing in their chemical and physical properties and the rate at which they release particular amino acids. The major use of carboxypeptidases in molecular biology is in the determination of the C作者: FLIP 時間: 2025-3-30 05:43 作者: 無目標 時間: 2025-3-30 08:44
https://doi.org/10.1007/978-3-658-42460-2active at an alkaline pH. The enzymes have been isolated from a variety of sources, including bacteria, fungi, invertebrates, fish, and mammals (being located in many organs, including bone marrow, kidney, placenta, and intestinal mucosa), but have not been isolated from higher plants. The most comm作者: 愛了嗎 時間: 2025-3-30 14:55
Use of Tools in Agile Projects,alyzes the template-directed polymerization of dNTPs at high temperatures. . When . polymerase was isolated in 1976, nobody could have known the impact the application of . polymerase to the polymerase chain reaction (PCR) would have on molecular biology (.,.). In an issue of . in 1989, the enzyme was dubbed molecule of the year (.).作者: 聯(lián)邦 時間: 2025-3-30 18:06 作者: 開始沒有 時間: 2025-3-30 22:22
https://doi.org/10.1007/978-1-4842-7161-2 identified in many prokaryotic organisms and are considered to be part of a defense system directed against foreign DNA. Site-specific endodeoxyribonucleases are given EC numbers, e.g., EC 3.1.21.4 for a Type II enzyme ..作者: 暖昧關(guān)系 時間: 2025-3-31 02:34
, Polymerase (EC 2.7.7.7),alyzes the template-directed polymerization of dNTPs at high temperatures. . When . polymerase was isolated in 1976, nobody could have known the impact the application of . polymerase to the polymerase chain reaction (PCR) would have on molecular biology (.,.). In an issue of . in 1989, the enzyme was dubbed molecule of the year (.).作者: CEDE 時間: 2025-3-31 08:32 作者: 征服 時間: 2025-3-31 10:30 作者: 漂亮才會豪華 時間: 2025-3-31 15:30 作者: 水土 時間: 2025-3-31 18:45
Kelly A. Basile,T. Alexandra BeauregardLigases are a class of enzymes that catalyze the joining of nucleic acid molecules by the formation of phosphodiester bonds between their termini (.). The nucleic acid substrate to be linked may be DNA or RNA depending on the type of ligase involved.作者: Admonish 時間: 2025-3-31 22:20
Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6),DNA-dependent RNA polymerases (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) catalyze the synthesis of RNA from ribonucleoside triphosphates in the presence of a DNA template and divalent cation (.).作者: Figate 時間: 2025-4-1 03:39 作者: convert 時間: 2025-4-1 08:44 作者: 橡子 時間: 2025-4-1 12:52
Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1),ses that preferentially catalyze the hydrolysis of double-stranded DNA (ds DNA) and that have found a use in the various techniques employed in molecular biology, The Type II restriction endonucleases obviously fall into this category, however they are a large subject on their own and therefore are 作者: laparoscopy 時間: 2025-4-1 16:39 作者: 遺留之物 時間: 2025-4-1 21:58 作者: Dendritic-Cells 時間: 2025-4-2 00:47
Reverse Transcriptase (EC 2.7.7.49), first in this process, retroviral RNA-directed DNA polymerase (reverse transcriptase), catalyzes the synthesis of a DNA copy of an RNA template in the presence of a suitable primer. Reverse transcriptase (RT) was discovered in 1970 (.,.) and was first used to copy a eukaryotic cell mRNA in 1971 (.–作者: oxidize 時間: 2025-4-2 06:43
Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31),ynucleotide primers. Different from all the other known DNA polymerases, TdT-catalyzed DNA synthesis is not template-directed. The reaction can be described as: . Only one dNTP is necessary for polymerization, although any dNTP (and derivatives thereof) or any combination of dNTPs will serve as subs作者: nonradioactive 時間: 2025-4-2 07:41
Restriction Enzymes, identified in many prokaryotic organisms and are considered to be part of a defense system directed against foreign DNA. Site-specific endodeoxyribonucleases are given EC numbers, e.g., EC 3.1.21.4 for a Type II enzyme ..作者: diskitis 時間: 2025-4-2 13:29
