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標(biāo)題: Titlebook: Enzymes in Nonaqueous Solvents; Methods and Protocol Evgeny N. Vulfson,Peter J. Halling,Herbert L. Holl Book 2001 Humana Press 2001 biochem [打印本頁]

作者: 粗野的整個    時間: 2025-3-21 18:05
書目名稱Enzymes in Nonaqueous Solvents影響因子(影響力)




書目名稱Enzymes in Nonaqueous Solvents影響因子(影響力)學(xué)科排名




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書目名稱Enzymes in Nonaqueous Solvents網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Enzymes in Nonaqueous Solvents被引頻次




書目名稱Enzymes in Nonaqueous Solvents被引頻次學(xué)科排名




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書目名稱Enzymes in Nonaqueous Solvents讀者反饋




書目名稱Enzymes in Nonaqueous Solvents讀者反饋學(xué)科排名





作者: mendacity    時間: 2025-3-21 21:02
Imprinting Enzymes for Use in Organic Mediactrostatic and hydrogen-bonding interactions among the surface residues of the protein in organic solvents (.). Despite this rigidity, enzymes remain active and selective in organic solvents, and this has led to a large number of applications that have impacted the chemical, pharmaceutical, and poly
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作者: prosthesis    時間: 2025-3-23 06:56
Very High Activity Biocatalysts for Low-Water Systems of magnitude. Because this can make the difference between whether a particular biotransformation is practically useful or not, it is important to control enzyme history carefully. The basic problem is simple: how to transfer the enzyme from an aqueous environment to one where it is dehydrated whil
作者: 含糊其辭    時間: 2025-3-23 10:22
Methods for Measurement and Control of Water in Nonaqueous Biocatalysisic) media. Most biocatalysts are inactive if fully dehydrated, and the reaction rate is stimulated by increasing hydration, at least at first. The rate of the desired reaction can become slower again if water levels increase too much, particularly when a hydrolytic side reaction becomes significant.
作者: Inveterate    時間: 2025-3-23 16:06

作者: 耐寒    時間: 2025-3-23 18:15
Water Activity Control for Lipase-Catalyzed Reactions in Nonaqueous Mediaemoving the water produced during the reaction. Many researchers have proposed several methods for water removal, such as headspace evacuation (.,.), pervaporation (.), use of molecular sieve (.), salt hydrate pairs (.,.), saturated salt solution, adsorption (.), and sparging of dry inert gas throug
作者: –LOUS    時間: 2025-3-23 23:31
Immobilization of Enzymes and Control of Water Activity in Low-Water Mediaticles with controlled pore sizes (.). This type of porous Celite differs from Celite powder in its capacity to adsorb water (more than 90% of Celite weight). Recently, it has been demonstrated that Celite R-640 in organic solvent adsorbs and releases water such that water activity (a.) is maintaine
作者: Ataxia    時間: 2025-3-24 05:57
Enzyme Activity and Enantioselectivity Measurements in Organic Mediahe solvent and the presence of water on enzyme activity and stereoselectivity has been studied extensively (.-.). New tools and methods had to be developed to undertake these studies. In the present chapter, we will describe some methods that were developed in our laboratory to measure the rates of
作者: 嬉耍    時間: 2025-3-24 08:11
Calorimetric Methods in Evaluating Hydration and Solvation of Solid Proteins Immersed in Organic Sol may be drastically changed by an organic solvent. Considering the variety of opportunities in use of different nonaqueous media, correct selection of organic environments presents a great challenge to biotechnological scientists and engineers. Such a selection needs the understanding of molecular m
作者: 商店街    時間: 2025-3-24 12:40

作者: 興奮過度    時間: 2025-3-24 16:42
The Effects of Crown Ethers on the Activity of Enzymes in Organic Solventsrther boosted by the recognition that enzymes can operate in organic solvents. The use of nonaqueous media for enzymatic conversions offers a number of advantages, like enhanced thermal stability of the enzyme, increased substrate solubility, a shift of the equilibrium in favor of synthesis over hyd
作者: Meditative    時間: 2025-3-24 20:08
Bieterpr?sentationen organisierenr catalytic activity at synthetically useful levels. In these cases, conventional aqueous-organic two-phase systems are still widely used, although the rates of mass transfer and inactivation of biocatalysts at the interface can be a serious drawback (.-.).
作者: Flustered    時間: 2025-3-25 02:01

作者: 可觸知    時間: 2025-3-25 05:42
Karen Heard-Lauréote,Carina Buckleythe importance of retaining native structure is well recognized, potential detrimental changes to the protonation state are often not considered. Usually, it is assumed that the enzyme will exhibit “pH memory” of the previous aqueous solution. As discussed in detail in Chapter 19, this is not always a valid assumption.
作者: 確定方向    時間: 2025-3-25 08:49

作者: considerable    時間: 2025-3-25 13:30
Agile Structures: Scaling Agilitystudied, but the optimal water activity was virtually the same in all solvents (.). Similarly, the optimal water activity was the same when different support materials were used for immobilization of the enzyme but the optimal water concentration varied depending on the water-absorbing capacity of the support (.).
作者: 妨礙議事    時間: 2025-3-25 17:12
Microencapsulation of Enzymes and Cells for Nonaqueous Biotransformationsr catalytic activity at synthetically useful levels. In these cases, conventional aqueous-organic two-phase systems are still widely used, although the rates of mass transfer and inactivation of biocatalysts at the interface can be a serious drawback (.-.).
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作者: laparoscopy    時間: 2025-3-26 03:37
Very High Activity Biocatalysts for Low-Water Systemsthe importance of retaining native structure is well recognized, potential detrimental changes to the protonation state are often not considered. Usually, it is assumed that the enzyme will exhibit “pH memory” of the previous aqueous solution. As discussed in detail in Chapter 19, this is not always a valid assumption.
作者: 煞費(fèi)苦心    時間: 2025-3-26 06:11

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作者: 最高峰    時間: 2025-3-26 16:28
Book 2001ne chemicals, and food ingredients. The high selectivity and mild reaction con- tions associated with enzymatic transformations have made this approach an attractive alternative in the synthesis of complex bioactive compounds, which are often difficult to obtain by standard chemical routes. However,
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作者: heart-murmur    時間: 2025-3-27 04:32
https://doi.org/10.1007/978-3-031-04212-6echanisms governing the interactions of proteins in nonaqueous media. This understanding should help also in elucidating the mechanisms controlling the conformational stability of the protein macromolecules, protein aggregation in solid state, aging of the protein preparation, and solubility of proteins in nonaqueous media.
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作者: 大門在匯總    時間: 2025-3-27 16:09
Excel-Kompetenz statt Copy-Paste-Reporting,n increase in active-site polarity, which correlates closely with a sharp increase in enzyme activity (.). The correlation between increased activity and increased active-site polarity suggests various strategies for activating enzymes in organic media.
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Detection of Structural Changes of Enzymes in Nonaqueous Media by Fluorescence and CD Spectroscopyis reasonably assumed that changes in activity and specificity of enzymes are mainly ascribed to changes in the higher structures of enzymes. Therefore, analysis of enzyme structure is important for the selection of the solvent, but methods to analyze dispersed or immobilized enzymes in organic solvents are rather limited.
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作者: esthetician    時間: 2025-3-29 07:04
Evolution of Project Managementctrostatic and hydrogen-bonding interactions among the surface residues of the protein in organic solvents (.). Despite this rigidity, enzymes remain active and selective in organic solvents, and this has led to a large number of applications that have impacted the chemical, pharmaceutical, and poly
作者: 加花粗鄙人    時間: 2025-3-29 09:35
Agile Development with ICONIX Processof photo-crosslinkable resin prepolymers having photosensitive functional groups, irradiation of near-ultraviolet (UV) light (maximum intensity at 365 nm) induces radical polymerization of the prepolymers and gel formation completes within a few minutes. Various types of prepolymers with different c
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作者: 瘋狂    時間: 2025-3-29 19:35
From Ad Hoc to Agility: A General Framework,orbed onto insoluble materials, copolymerized with a reactive monomer, encapsulated in gels, crosslinked with a bifunctional reagent, covalently bound to an insoluble carrier (.) or entrapped within an insoluble gel matrix of natural or synthetic resin (.).
作者: 連接    時間: 2025-3-29 23:11
https://doi.org/10.1007/978-3-658-41332-3r the purpose of applying proteins to biomedical and biotechnological processes (.-.). In the biomedical field, the purpose of the chemical modification of enzymes with PEG includes the reduction of their immunoreactivity and immunogenicity as well as the prolongation of the plasma half-life of the
作者: 污穢    時間: 2025-3-30 00:04

作者: 官僚統(tǒng)治    時間: 2025-3-30 04:34
https://doi.org/10.1007/978-981-10-3358-2ic synthesis and numerous examples also in the fields of food-related conversions and analysis. However, enzymes show a lower catalytic efficiency (up to three or four orders of magnitude) when employed in organic solvents rather than in aqueous buffer. It is evident that such behavior represents an
作者: 違法事實(shí)    時間: 2025-3-30 12:11

作者: facilitate    時間: 2025-3-30 16:20
Karen Heard-Lauréote,Carina Buckley of magnitude. Because this can make the difference between whether a particular biotransformation is practically useful or not, it is important to control enzyme history carefully. The basic problem is simple: how to transfer the enzyme from an aqueous environment to one where it is dehydrated whil
作者: Meager    時間: 2025-3-30 19:34

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作者: 他一致    時間: 2025-3-31 04:32
,Die Marke als Wertsch?pfungsfaktor,emoving the water produced during the reaction. Many researchers have proposed several methods for water removal, such as headspace evacuation (.,.), pervaporation (.), use of molecular sieve (.), salt hydrate pairs (.,.), saturated salt solution, adsorption (.), and sparging of dry inert gas throug
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作者: slow-wave-sleep    時間: 2025-3-31 13:06
In- Versus Outsourcing and Vendor Selection,he solvent and the presence of water on enzyme activity and stereoselectivity has been studied extensively (.-.). New tools and methods had to be developed to undertake these studies. In the present chapter, we will describe some methods that were developed in our laboratory to measure the rates of
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https://doi.org/10.1385/1592591124biochemical engineering
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