作者: 丑惡 時(shí)間: 2025-3-21 20:27 作者: doxazosin 時(shí)間: 2025-3-22 03:13 作者: Creditee 時(shí)間: 2025-3-22 06:36
Chemoenzymatic Synthesis of Linear- and Head-to-Tail Cyclic Peptides Using Omniligase-1,peptides. Reactions are irreversible and proceed with unprotected peptides (μM–mM?concentration) in aqueous solution at slightly basic pH. Due to its high catalytic efficiency, only very low molar equivalents of omniligase-1 are required. In this chapter, a chemoenzymatic peptide synthesis (CEPS) ap作者: cuticle 時(shí)間: 2025-3-22 10:39
Site-Specific Labeling of Proteins Using the Formylglycine-Generating Enzyme (FGE),directly from nature of introducing a unique amino acid into the larger context of a protein. Formylglycine (fGly) is a crucial component of the active site of sulfatases, where it directly participates in the breakdown of sulfate ester substrates. In the context of bioconjugation this aldehyde cont作者: Scleroderma 時(shí)間: 2025-3-22 15:21
Butelase 1-Mediated Ligation of Peptides and Proteins,Asx) ligase activity and is virtually devoid of protease activity. Butelase 1 recognizes specifically a .-terminal Asx-containing tripeptide motif, Asx-His-Val, to form an Asx-Xaa peptide bond (Xaa?=?any amino acid), either intramolecularly or intermolecularly, resulting in cyclic peptides or site-s作者: Scleroderma 時(shí)間: 2025-3-22 17:51
Trypsiligase-Catalyzed Peptide and Protein Ligation,sents a major challenge to protein chemists. Chemical labeling methods often target multiple positions within a protein and therefore suffer from a lack of specificity. Enzymatic protein modification is an attractive alternative due to the inherent regioselectivity and stereoselectivity of enzymes. 作者: 常到 時(shí)間: 2025-3-23 00:19 作者: neutralize 時(shí)間: 2025-3-23 03:50 作者: evanescent 時(shí)間: 2025-3-23 07:36
SpyLigase-Catalyzed Modification of Antibodies,ifferent cell populations as well as for tracking intracellular pathways. In recent years, antibody–drug conjugates (ADCs) have emerged as promising therapeutics to treat cancer and have moved into the focus of interest of the pharmaceutical industry. Here we describe a conjugation method for the ge作者: reception 時(shí)間: 2025-3-23 11:02 作者: 遭受 時(shí)間: 2025-3-23 14:20
In Vitro and , Cyclization of Target Peptides Using an Asparaginyl Endopeptidase from ,ty or potential oral bioavailability. However, there are few tools available for carrying out this modification. Asparaginyl endopeptidases (AEPs) are a class of enzymes that typically work as proteases, but a subset is highly efficient at cyclization of the peptide backbone. In this chapter we desc作者: 彈藥 時(shí)間: 2025-3-23 20:44 作者: 甜得發(fā)膩 時(shí)間: 2025-3-24 00:51 作者: Oafishness 時(shí)間: 2025-3-24 03:51
BioID as a Tool for Protein-Proximity Labeling in Living Cells,romiscuous biotin ligase, called BioID, fused to a protein of interest that when expressed in cells can be induced to biotinylate interacting and proximate proteins over a period of hours, thus generating a history of protein associations. These biotinylated proteins are subsequently purified and id作者: adj憂郁的 時(shí)間: 2025-3-24 08:37 作者: rheumatology 時(shí)間: 2025-3-24 12:30 作者: FLEET 時(shí)間: 2025-3-24 17:57 作者: 出汗 時(shí)間: 2025-3-24 22:53 作者: 涂掉 時(shí)間: 2025-3-24 23:34
,Reprisals in Ireland, 1919–1921,ecursor peptide are coexpressed in the leaves of the model plant ., and cyclization of the target peptide occurs .. Using the in vitro method, purified recombinant OaAEP1. produced in bacteria is used to cyclize the target precursor peptide in vitro.作者: 夸張 時(shí)間: 2025-3-25 05:44 作者: Paradox 時(shí)間: 2025-3-25 08:42
Chemoenzymatic Synthesis of Linear- and Head-to-Tail Cyclic Peptides Using Omniligase-1, we provide protocols for the chemoenzymatic synthesis of the peptide therapeutic exenatide, a GLP-1 (glucagon-like peptide) analogue, and the macrocyclization and oxidative folding of the cyclotide MCoTI-II in a one-pot procedure.作者: NAIVE 時(shí)間: 2025-3-25 12:25 作者: 不感興趣 時(shí)間: 2025-3-25 15:56
BioID as a Tool for Protein-Proximity Labeling in Living Cells,d transient interactions within a relevant biological setting over a defined period of time. Here we briefly review the scientific progress enabled by the BioID technology, detail an updated protocol for applying the method to proteins in living cells, and offer insights for troubleshooting commonly encountered setbacks.作者: 高腳酒杯 時(shí)間: 2025-3-25 21:28
Cyclizing Disulfide-Rich Peptides Using Sortase A, application of SrtA as a tool for the cyclization of disulfide-rich peptides. Reactions are typically tailored to each disulfide-rich peptide with optimal conditions producing yields of 40–50% cyclized peptide.作者: Immortal 時(shí)間: 2025-3-26 03:21 作者: Inflated 時(shí)間: 2025-3-26 08:15 作者: foppish 時(shí)間: 2025-3-26 08:56 作者: BATE 時(shí)間: 2025-3-26 15:50
https://doi.org/10.1007/978-3-319-59132-2abeling, preparation of thioesters, and bioconjugation of dendrimers. Additionally, we provide an example using butelase 1 for protein cyclization in combination with genetic code expansion in order to incorporate unnatural building blocks.作者: 分貝 時(shí)間: 2025-3-26 19:47 作者: hurricane 時(shí)間: 2025-3-27 00:42 作者: 值得尊敬 時(shí)間: 2025-3-27 01:39
Is Caste Already Part of UK Equality Law?,in imaging and diagnostics. Here we describe the modification of proteins using TTL in detail via a one-step as well as two-step procedure and highlight its practicability for applications in imaging, diagnostics, and cell biology.作者: insurrection 時(shí)間: 2025-3-27 06:00
Butelase 1-Mediated Ligation of Peptides and Proteins,abeling, preparation of thioesters, and bioconjugation of dendrimers. Additionally, we provide an example using butelase 1 for protein cyclization in combination with genetic code expansion in order to incorporate unnatural building blocks.作者: Excise 時(shí)間: 2025-3-27 10:57
SpyLigase-Catalyzed Modification of Antibodies,rom . cells, chemical solid-phase synthesis of the KTag peptide and its coupling to reporter molecules and cytotoxins as well as the transient expression from mammalian cells to produce Spy-tagged antibodies. Furthermore, we describe the purification and analytics of the formed conjugates.作者: CLASH 時(shí)間: 2025-3-27 14:24
Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation, handle that is then used for drug molecule attachment in a second step. The aim of this chapter is to outline detailed protocols for both labeling procedures, as well as to provide guidance on enzyme and substrate preparation.作者: STIT 時(shí)間: 2025-3-27 21:00 作者: 重力 時(shí)間: 2025-3-28 01:02
Book 2019to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial tra作者: fabricate 時(shí)間: 2025-3-28 02:58
1064-3745 ation advice from the experts.This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as s作者: overweight 時(shí)間: 2025-3-28 06:27
After-Sales-Feedback mit Kundenkonferenzenaction conditions. In this chapter, we describe proximity-based sortase-mediated ligation (PBSL), which uses the SpyTag–SpyCatcher peptide–protein pair to link SrtA to target proteins and dramatically improves reaction rate and ligation efficiency.作者: 極大痛苦 時(shí)間: 2025-3-28 10:55
https://doi.org/10.1007/978-981-10-7092-1 .terminal NMT recognition sequence are covalently modified with an azido fatty acid. Subsequent strain-promoted azide–alkyne cycloaddition allows for conjugation to cell-permeant fluorophores and imaging by fluorescence microscopy. Here we describe sample preparation and labeling protocols for imaging bacterial proteins in fixed and live cells.作者: glans-penis 時(shí)間: 2025-3-28 18:37 作者: 整頓 時(shí)間: 2025-3-28 22:10 作者: 不法行為 時(shí)間: 2025-3-29 02:23 作者: covert 時(shí)間: 2025-3-29 03:28 作者: Microaneurysm 時(shí)間: 2025-3-29 08:37
Le Carré Landscapes: The Cold Warheterologous expression and purification of these enzymes as well as performing in vitro biochemical reactions. Finally, approaches to recover the final product from an enzymatic reaction mixture are also discussed.作者: medieval 時(shí)間: 2025-3-29 13:12
Fabiola H. Gerpott,Alfred Kiesere describe a protocol to perform SPOCQ reaction on proteins, along with notes to optimize yield and reaction rates. Conjugation efficiencies of over 95% to antibodies have been reported using this protocol.作者: 彩色 時(shí)間: 2025-3-29 17:54 作者: obsolete 時(shí)間: 2025-3-29 22:47
Enzyme-Mediated Ligation Methods978-1-4939-9546-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 不在灌木叢中 時(shí)間: 2025-3-30 00:49 作者: 無(wú)法破譯 時(shí)間: 2025-3-30 07:47
After-Sales-Feedback mit Kundenkonferenzeng an LPXTG SrtA recognition motif. Unfortunately, SrtA suffers from low turnover rates, resulting in poor ligation efficiencies even with optimized reaction conditions. In this chapter, we describe proximity-based sortase-mediated ligation (PBSL), which uses the SpyTag–SpyCatcher peptide–protein pai作者: 放逐某人 時(shí)間: 2025-3-30 09:38 作者: liaison 時(shí)間: 2025-3-30 13:00
Elaine Clanton Harpine,Adam Pazdapeptides. Reactions are irreversible and proceed with unprotected peptides (μM–mM?concentration) in aqueous solution at slightly basic pH. Due to its high catalytic efficiency, only very low molar equivalents of omniligase-1 are required. In this chapter, a chemoenzymatic peptide synthesis (CEPS) ap作者: 綁架 時(shí)間: 2025-3-30 18:04
https://doi.org/10.1007/978-3-319-59141-4directly from nature of introducing a unique amino acid into the larger context of a protein. Formylglycine (fGly) is a crucial component of the active site of sulfatases, where it directly participates in the breakdown of sulfate ester substrates. In the context of bioconjugation this aldehyde cont作者: 頌揚(yáng)國(guó)家 時(shí)間: 2025-3-30 21:35
https://doi.org/10.1007/978-3-319-59132-2Asx) ligase activity and is virtually devoid of protease activity. Butelase 1 recognizes specifically a .-terminal Asx-containing tripeptide motif, Asx-His-Val, to form an Asx-Xaa peptide bond (Xaa?=?any amino acid), either intramolecularly or intermolecularly, resulting in cyclic peptides or site-s作者: aspect 時(shí)間: 2025-3-31 02:27 作者: 門窗的側(cè)柱 時(shí)間: 2025-3-31 06:17 作者: olfction 時(shí)間: 2025-3-31 10:31 作者: DECRY 時(shí)間: 2025-3-31 14:55 作者: judicial 時(shí)間: 2025-3-31 18:16 作者: vector 時(shí)間: 2025-4-1 00:52
