標題: Titlebook: Enzyme-Mediated Immunoassay; T. T. Ngo,H. M. Lenhoff Book 1985 Plenum Press, New York 1985 Pet.Plasma.Vine.antibody.biology.cell.cell biol [打印本頁] 作者: 吸收 時間: 2025-3-21 16:16
書目名稱Enzyme-Mediated Immunoassay影響因子(影響力)
書目名稱Enzyme-Mediated Immunoassay影響因子(影響力)學科排名
書目名稱Enzyme-Mediated Immunoassay網(wǎng)絡公開度
書目名稱Enzyme-Mediated Immunoassay網(wǎng)絡公開度學科排名
書目名稱Enzyme-Mediated Immunoassay被引頻次
書目名稱Enzyme-Mediated Immunoassay被引頻次學科排名
書目名稱Enzyme-Mediated Immunoassay年度引用
書目名稱Enzyme-Mediated Immunoassay年度引用學科排名
書目名稱Enzyme-Mediated Immunoassay讀者反饋
書目名稱Enzyme-Mediated Immunoassay讀者反饋學科排名
作者: fructose 時間: 2025-3-21 22:52
https://doi.org/10.1057/9780230355484s in biomedical sciences and has become a standard tool in laboratory medicine. Millions of clinical tests are now routinely performed by using RIA. The rapid and wide acceptance of RIA as a routine clinical method can be attributed to (1) the general applicability of the method, i.e., any compound 作者: Albinism 時間: 2025-3-22 03:06
https://doi.org/10.1007/978-1-349-24708-0that binding of an anti-drug antibody to an enzyme labeled with the same drug, modulated (inhibited or activated) catalytic activity. Thus a competitive immunoassay can be developed in which drug in the sample and enzyme-labeled drug compete for antibody. Modulation of enzyme activity is directly re作者: 下船 時間: 2025-3-22 08:22
https://doi.org/10.1007/978-3-662-62325-1label a ligand analyte (L). Such stable covalent enzyme modulator-ligand conjugates (M-L) is capable of modulating the activity of an indicator enzyme by either causing a significant inhibition of the enzyme activity or a dramatic activation.作者: 救護車 時間: 2025-3-22 09:28 作者: amphibian 時間: 2025-3-22 15:45
https://doi.org/10.1007/978-3-658-04422-0also known as Substrate-labeled Fluorescent Immunoassay) uses a modified fluorogenic enzyme substrate as a label to form a stable covalent substrate-analyte ligand conjugate. This is in contrast to most enzyme mediated immunoassays which use an enzyme rather than an enzyme substrate as the label (Ng作者: amphibian 時間: 2025-3-22 20:51 作者: Notorious 時間: 2025-3-22 23:55
Central Asia: The Illusion of a World Orderse vesicles can be prepared as large multilamellar structures containing many internal aqueous compartments or as smaller unilamellar structures with only one internal compartment. If prepared in the presence of marker molecules, such as soluble enzymes, ions or flurophores, the markers are entrappe作者: DECRY 時間: 2025-3-23 05:09
Laboratory based EIA methods have found important applications in the areas of clinical chemistry, veterinary medicine, microbiology, and human fertility. The great versatility of these methods, for both the identification and quantitation of clinically relevant antigens, is illustrated by example 作者: Nomadic 時間: 2025-3-23 08:04
https://doi.org/10.1057/9780230274044ng with the inherently better speed, stability, and cost have resulted in the commercial production of several EIA systems (Monroe, 1984). Most systems have colorimet-rically monitored the indicating enzyme reaction. With the appropriate considerations, a fluorescence monitor can further enhance the作者: 冰河期 時間: 2025-3-23 12:05 作者: GRATE 時間: 2025-3-23 17:27 作者: affect 時間: 2025-3-23 20:37 作者: CHART 時間: 2025-3-23 23:50
Eyewitness Identification by Theft Victims,in contrast to competitive radioimmunoassays or enzyme-immunoassays in which labeled-antigens are utilized in competition with sample antigen to bind to a limited number of antibody sites. The label applied to the antibody can take the form of a radioisotope, enzyme, fluorophore, luminescent tag, or作者: Bronchial-Tubes 時間: 2025-3-24 05:16
Palgrave Macmillan Memory Studiesmaleimide derivatives have been used for coupling the enzyme to proteins, such as immunoglobulins and their fragments (Ishikawa, 1983). Use of these coupling agents showed no appreciable reduction of enzyme activity due to the involvement of sulfhydryl groups of the enzyme in the coupling process. C作者: wall-stress 時間: 2025-3-24 07:50 作者: 圣歌 時間: 2025-3-24 13:42 作者: 大包裹 時間: 2025-3-24 16:17 作者: dearth 時間: 2025-3-24 20:14
https://doi.org/10.1057/9780230305137We describe here an approach to separation-free enzyme immunoassays based on the ability of specific antibodies to ligands to restrict the conformational mobility of an apoenzyme that has already been labeled with that ligand, to fold into a catalytically active conformation.作者: burnish 時間: 2025-3-25 01:57 作者: Lipoma 時間: 2025-3-25 06:55
https://doi.org/10.1007/978-3-662-62325-1label a ligand analyte (L). Such stable covalent enzyme modulator-ligand conjugates (M-L) is capable of modulating the activity of an indicator enzyme by either causing a significant inhibition of the enzyme activity or a dramatic activation.作者: left-ventricle 時間: 2025-3-25 08:42
https://doi.org/10.1057/9780230355316lity of a stable covalent ligand-prosthetic group conjugate to activate an apoenzyme which by itself is enzymatically inactive; and (2) the ability of anti-ligand antibody to bind ligand-prosthetic group conjugate such that the bound ligand-prosthetic group is incapable of activating the apoenzyme.作者: Blasphemy 時間: 2025-3-25 12:45 作者: 鞠躬 時間: 2025-3-25 17:40 作者: Gourmet 時間: 2025-3-25 23:58 作者: 錢財 時間: 2025-3-26 02:55
Prosthetic Group Labeled Enzyme Immunoassaylity of a stable covalent ligand-prosthetic group conjugate to activate an apoenzyme which by itself is enzymatically inactive; and (2) the ability of anti-ligand antibody to bind ligand-prosthetic group conjugate such that the bound ligand-prosthetic group is incapable of activating the apoenzyme.作者: 鞠躬 時間: 2025-3-26 05:33
Nonseparation Enzyme Immunoassays for Macromoleculesdase together with a macromolecular substrate (Gibbons et al., 1980). The second is based on the phenomenon of “enzyme channeling” (Litman et al., 1980) involving two or more enzymes. This chapter will discuss the principles, characteristics, performance and clinical utility of both types of assay.作者: GLIDE 時間: 2025-3-26 09:11 作者: 發(fā)怨言 時間: 2025-3-26 15:55
http://image.papertrans.cn/e/image/313102.jpg作者: 反復無常 時間: 2025-3-26 20:39
978-1-4684-5014-9Plenum Press, New York 1985作者: 分散 時間: 2025-3-26 23:56
Palgrave Macmillan Memory Studiestivity (Comoglio and Celada, 1976). In order to obivate such disadvantages, we utilized m-maleimidobenzoyl derivative of a hapten in order to couple it to sulfhydryl groups of the enzyme; a high efficiency of binding to the enzyme without appreciable reduction in enzyme activity were found.作者: Hallowed 時間: 2025-3-27 04:38
Maleimide Derivative of Hapten for Enzyme Coupling in EIAtivity (Comoglio and Celada, 1976). In order to obivate such disadvantages, we utilized m-maleimidobenzoyl derivative of a hapten in order to couple it to sulfhydryl groups of the enzyme; a high efficiency of binding to the enzyme without appreciable reduction in enzyme activity were found.作者: 碎石頭 時間: 2025-3-27 09:17 作者: fiscal 時間: 2025-3-27 13:14
Separation-Free Enzyme Immunoassay for Haptenslated to the analyte concentration in the sample and is monitored without separation of antibody bound and unbound fractions. This principle was later extended to a variety of enzymes and also applied to the measurement of hormones (Ullman et al., 1979) and proteins (Gibbons et al., 1980).作者: CAPE 時間: 2025-3-27 16:26
Affinity Column Mediated Immunoenzymometric Assays almost any tag ultimately capable of generating a signal, even through some complex coupling mechanism. Two fundamentally different immunometric assay configurations have been described: the one-site immunometric assay and the two site (sandwich) immunometric assay.作者: 黃油沒有 時間: 2025-3-27 20:19 作者: 我不怕犧牲 時間: 2025-3-27 23:01 作者: 最高峰 時間: 2025-3-28 05:19
lity. The great versatility of these methods, for both the identification and quantitation of clinically relevant antigens, is illustrated by example throughout this volume and is largely attributable to the stability and amplification power of enzyme labels.作者: 友好 時間: 2025-3-28 09:41
https://doi.org/10.1057/9780230274044s have colorimet-rically monitored the indicating enzyme reaction. With the appropriate considerations, a fluorescence monitor can further enhance the sensitivity and selectivity of such methods without introducing significant sacrifices in the ease, speed, or cost of analysis (Kelly and Christian, 1982).作者: comely 時間: 2025-3-28 13:20
An American Worldview; An American World, i.e., sequential (noncompetitive) and competitive. The sequential, heterogenous immunoassay technique, otherwise known as the Enzyme Linked Immunosorbent Assay or ELISA, has been most widely adopted.作者: NIB 時間: 2025-3-28 15:23 作者: 圓桶 時間: 2025-3-28 20:34
Test Strip Enzyme Immunoassaylity. The great versatility of these methods, for both the identification and quantitation of clinically relevant antigens, is illustrated by example throughout this volume and is largely attributable to the stability and amplification power of enzyme labels.作者: FLAGR 時間: 2025-3-29 02:21 作者: 冬眠 時間: 2025-3-29 03:05 作者: chemical-peel 時間: 2025-3-29 08:03
https://doi.org/10.1057/9780230355484can be analyzed by a RIA so long as an antibody specific to that compound is available; (2) the selectivity and specificity of the method; (3) the sensitivity of a radioactive isotope as the label in RIA and (4) the minimal inference and ease of performing the test.作者: Between 時間: 2025-3-29 12:48 作者: BILL 時間: 2025-3-29 18:02
Eyewitness Identification by Theft Victims, almost any tag ultimately capable of generating a signal, even through some complex coupling mechanism. Two fundamentally different immunometric assay configurations have been described: the one-site immunometric assay and the two site (sandwich) immunometric assay.作者: 不可思議 時間: 2025-3-29 19:49
Book 1985ulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number o作者: 流出 時間: 2025-3-30 02:55 作者: 不可侵犯 時間: 2025-3-30 05:03 作者: Heart-Attack 時間: 2025-3-30 09:27 作者: 檢查 時間: 2025-3-30 15:02 作者: Misgiving 時間: 2025-3-30 17:56
https://doi.org/10.1007/978-3-030-43343-7ng the substrate reaction. The result is a system with improved sensitivity and broad flexibility. The a-ELISA serves as a useful means of measuring the isotypic distribution of specific immune responses in many species.作者: GROWL 時間: 2025-3-30 23:10
https://doi.org/10.1057/978-1-137-50956-7ght. Using fluorimetric microscopy, Rotman (1961) was able to detect single molecules of the enzyme β-galactosidase. Under more practical circumstances, the detection limits of fluorimetry are frequently limited by background fluorescence such as that arising from serum or other biological samples.作者: bourgeois 時間: 2025-3-31 04:49 作者: HAUNT 時間: 2025-3-31 08:37 作者: 短程旅游 時間: 2025-3-31 11:38
Fluorogenic Substrate Labeled Separation-Free Enzyme Mediated Immunoassays for Haptens and MacromoleBy definition, a fluorogenic enzyme substrate should not fluoresce at the wavelength used to monitor the assay; however, its enzymatic product should exhibit strong fluorescence at the appropriate monitoring wavelengths. The substrate-analyte ligand conjugate in FSIA serves as a modified enzyme subs作者: 混合 時間: 2025-3-31 13:33 作者: frozen-shoulder 時間: 2025-3-31 21:07 作者: 緯線 時間: 2025-3-31 23:51
