標題: Titlebook: Enzyme Engineering; Methods and Protocol Francesca Magnani,Chiara Marabelli,Francesca Parad Book 2022 The Editor(s) (if applicable) and The [打印本頁] 作者: 正當理由 時間: 2025-3-21 16:09
書目名稱Enzyme Engineering影響因子(影響力)
書目名稱Enzyme Engineering影響因子(影響力)學科排名
書目名稱Enzyme Engineering網(wǎng)絡(luò)公開度
書目名稱Enzyme Engineering網(wǎng)絡(luò)公開度學科排名
書目名稱Enzyme Engineering被引頻次
書目名稱Enzyme Engineering被引頻次學科排名
書目名稱Enzyme Engineering年度引用
書目名稱Enzyme Engineering年度引用學科排名
書目名稱Enzyme Engineering讀者反饋
書目名稱Enzyme Engineering讀者反饋學科排名
作者: GRUEL 時間: 2025-3-21 21:58
Resurrecting Enzymes by Ancestral Sequence Reconstructionunderstanding physicochemical properties of proteins, among others. In this context, the aim of this chapter is to present the ASR approach applied to the reconstruction of enzymes—.., proteins with catalytic roles. The spirit of this contribution is to provide a basic, hands-to-work guide for bioch作者: 切碎 時間: 2025-3-22 00:42 作者: commonsense 時間: 2025-3-22 04:55 作者: faultfinder 時間: 2025-3-22 11:31 作者: vasospasm 時間: 2025-3-22 12:58
Book 2022ly, .Enzyme Engineering: Methods and Protocols. is an ideal guide for both the novice and the veteran researcher interested in biocatalysis..Chapter 13 is available open access under a CC BY 4.0 license..作者: vasospasm 時間: 2025-3-22 20:31
Entering the Emotional Practices of Teaching the user makes about the likelihood of each recombination event, yet most currently available ASR tools provide limited, if any, opportunities for evaluating indel placement in a reconstructed sequence. Graphical Representation of Ancestral Sequence Predictions (GRASP) is an ASR tool that maps inde作者: CEDE 時間: 2025-3-22 23:54 作者: 變白 時間: 2025-3-23 01:22 作者: 疏忽 時間: 2025-3-23 06:43 作者: 嚴厲批評 時間: 2025-3-23 11:25
Marcella Niglio,Cosimo Damiano Vitalete entrance tunnels in order to fine-tune enzymatic activity, regulate substrate specificity, or control reaction promiscuity..In this chapter, we describe the use of a rational in silico design and screening method to engineer the access tunnel of a fructosyl peptide oxidase with the aim to facilit作者: fiscal 時間: 2025-3-23 15:18
1064-3745 and timely, .Enzyme Engineering: Methods and Protocols. is an ideal guide for both the novice and the veteran researcher interested in biocatalysis..Chapter 13 is available open access under a CC BY 4.0 license..978-1-0716-1828-8978-1-0716-1826-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 厭惡 時間: 2025-3-23 19:29 作者: bifurcate 時間: 2025-3-23 23:34 作者: 土產(chǎn) 時間: 2025-3-24 04:15 作者: Pseudoephedrine 時間: 2025-3-24 09:10 作者: 激怒某人 時間: 2025-3-24 12:56
Recombination of Compatible Substitutions by 2GenReP and InSiRePic properties. However, the question of how to efficiently explore the protein sequence space when ten or more beneficial substitutions are identified has not yet been addressed. Two recombination strategies 2GenReP and InSiReP employing CompassR are systematically investigated to minimize experimen作者: opalescence 時間: 2025-3-24 16:29 作者: corpus-callosum 時間: 2025-3-24 19:12
Resurrecting Enzymes by Ancestral Sequence Reconstructionisclosing the evolutionary history—.., the phylogeny—of a protein family of interest and then inferring the sequences of its ancestors—.., the nodes in the phylogeny. Assisted by gene synthesis, the selected ancestors can be resurrected in the lab and experimentally characterized. The crucial step t作者: comely 時間: 2025-3-25 01:48 作者: 種屬關(guān)系 時間: 2025-3-25 05:35
Rational-Design Engineering to Improve Enzyme Thermostabilityrehension of the thermal stability regulating factors of proteins is needful for the engineering of their thermostability. Protein engineering aims to overcome their natural limitations in tough conditions by refining protein stability and activity. Rational-design approach requires a crystal struct作者: 逗它小傻瓜 時間: 2025-3-25 07:46 作者: 性上癮 時間: 2025-3-25 12:44
In Silico Engineering of Enzyme Access Tunnelscy, stability, or specificity. By introducing partial modifications to their sequence and to their structural features, enzyme engineering can transform natural enzymes into more efficient, specific and resistant biocatalysts and render them suitable for virtually countless industrial processes. Cur作者: 頌揚國家 時間: 2025-3-25 16:01
Quantum-Mechanical/Molecular-Mechanical (QM/MM) Simulations for Understanding Enzyme Dynamicspproaches, a portion of the enzyme of great interest (e.g., where a chemical reaction is occurring) is treated with QM, whereas the surrounding region is treated with MM. A critical challenge with these methods is the choice of the region to partition into QM and which to treat with MM along with nu作者: jagged 時間: 2025-3-25 21:22
Computational Enzyme Design at Zymvolce exploration; both require screening of thousands (if not millions) of variants to achieve a target function. Computer-driven approaches can limit laboratorial screening to a few hundred candidates, enabling and accelerating the development of industrial enzymes. In this book chapter, the technolo作者: CHANT 時間: 2025-3-26 01:43
Advanced Enzyme Immobilization Technologies: An Eco-friendly Support, a Polymer-Stabilizing Immobilition from the final product. However, none of them have reached a generalized implementation and new immobilization technologies are continuously being developed to improve the properties of the immobilized biocatalysts. In this chapter, we describe three advanced strategies looking at the key point作者: 評論者 時間: 2025-3-26 04:54
Book 2022bing library preparation and screening, state of the art techniques in directed evolution and rational design, followed by examples of immobilization of enzymes on sustainable polymers, as well as biocatalytic conversions mediated by homogenous enzymatic preparations or whole cells. Written for the 作者: Charitable 時間: 2025-3-26 09:50
Preparation of Soil Metagenome Libraries and Screening for Gene-Specific Amplicons Here we describe methods for the construction of a cosmid metagenome library from a soil sample, and the process of screening that library for individual cosmid clones containing aromatic polyketide BGCs using degenerate primers that target the ketosynthase alpha (KSα) gene.作者: Pepsin 時間: 2025-3-26 15:22 作者: Microaneurysm 時間: 2025-3-26 18:23
1064-3745 expertsThis book provides comprehensive methods and protocols about enzyme design. The chapters are grouped by main topic, starting with methodologies describing library preparation and screening, state of the art techniques in directed evolution and rational design, followed by examples of immobil作者: FLAIL 時間: 2025-3-27 00:41
Advances in Swine in Biomedical Researchibosyl transferases using FACS as a more widespread and accessible alternative than microfluidic on-chip sorters. This protocol can be easily adapted to directed evolution libraries by replacing the library construction steps and to other enzyme activities, e.g., oxidases, by replacing the proposed coupled assay.作者: 昆蟲 時間: 2025-3-27 02:58 作者: 變白 時間: 2025-3-27 07:23
Markku Ohenoja,Marko Paavola,Kauko Leivisk?n-ligand interaction fingerprinting analysis, to identify specific positions for site-saturation mutagenesis toward manipulating substrate specificity/stereoselectivity of an alcohol dehydrogenase, and improving activity of a carboxylic acid reductase, respectively.作者: 大范圍流行 時間: 2025-3-27 09:39 作者: deceive 時間: 2025-3-27 15:38
https://doi.org/10.1007/978-981-15-9877-7nvolved in preparing protein structures, choosing the appropriate QM region size and electronic structure methods, preparing all necessary input files, and troubleshooting common errors for QM/MM simulations of enzymes.作者: Organonitrile 時間: 2025-3-27 21:00 作者: Priapism 時間: 2025-3-28 01:19
Recombination of Compatible Substitutions by 2GenReP and InSiRePbstitutions and discuss some important practical issues that should be considered for the application of 2GenReP and InSiReP, such as placing the substitutions into subsets. The core part of the protocol (Step1 to Step5) is transferable to other enzymes and any recombination of potential substitutions.作者: organic-matrix 時間: 2025-3-28 05:15 作者: 變態(tài) 時間: 2025-3-28 08:26 作者: 羊齒 時間: 2025-3-28 14:13
Computational Enzyme Design at Zymvolaboratorial screening to a few hundred candidates, enabling and accelerating the development of industrial enzymes. In this book chapter, the technology adopted at Zymvol is described. An overview of the current development and future directions in the company is also provided.作者: 膝蓋 時間: 2025-3-28 18:16 作者: Density 時間: 2025-3-28 20:35
https://doi.org/10.1007/978-3-319-93946-9gned. On the contrary, the enhanced quality of synthetic DNA libraries makes the exploration of sequence space more efficient. Here, methods for the effective utilization of synthetic DNA libraries are described. The overall procedure allows the generation of ready-to-screen libraries within two weeks from synthetic DNA acquisition.作者: 清晰 時間: 2025-3-28 23:02 作者: 全能 時間: 2025-3-29 03:33
Sri Addepalli,Yifan Zhao,Lawrence Tinsleylymer is used during the enzyme immobilization procedure to prevent the subunit dissociation of multimeric enzymes as well as to avoid excessive rigidification of the covalently immobilized enzyme. Finally, the reversible co-immobilization of cofactors has been improved by increasing the reactive groups of the support.作者: 爆米花 時間: 2025-3-29 10:41
Expression and In Vivo Loading of De Novo Proteins with Tetrapyrrole Cofactorsns in vivo. In addition, we describe the imaging of hydrophobic proteins and cofactor-rich protein droplets by electron and fluorescence microscopy, and how cofactors can be stripped from the de novo proteins to aid in vitro identification.作者: 大氣層 時間: 2025-3-29 13:39
Advanced Enzyme Immobilization Technologies: An Eco-friendly Support, a Polymer-Stabilizing Immobililymer is used during the enzyme immobilization procedure to prevent the subunit dissociation of multimeric enzymes as well as to avoid excessive rigidification of the covalently immobilized enzyme. Finally, the reversible co-immobilization of cofactors has been improved by increasing the reactive groups of the support.作者: 身心疲憊 時間: 2025-3-29 18:25
Francesca Magnani,Chiara Marabelli,Francesca ParadIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 個阿姨勾引你 時間: 2025-3-29 20:36
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/313062.jpg作者: INTER 時間: 2025-3-29 23:52
https://doi.org/10.1007/978-1-4613-1229-1ties. The large insert size (～35?kb) of such libraries means they are compatible with downstream expression of large biosynthetic gene clusters (BGCs). This allows the discovery of previously undescribed natural products that would be inaccessible using traditional culture-based discovery pipelines.作者: Heart-Attack 時間: 2025-3-30 07:58
Advances in Swine in Biomedical Researchgent consumption and lower costs than conventional robotic alternatives. Here we describe an example of metagenomic screening for nucleoside 2′-deoxyribosyl transferases using FACS as a more widespread and accessible alternative than microfluidic on-chip sorters. This protocol can be easily adapted 作者: 我的巨大 時間: 2025-3-30 09:51 作者: BOOST 時間: 2025-3-30 13:04
Markku Ohenoja,Marko Paavola,Kauko Leivisk?mutagenesis, site-directed or site-saturation mutagenesis requires a semi-rational selection of the amino acid positions or the substituted residues, which can dramatically reduce the screening efforts in protein engineering. To this end, in silico prediction methods play a pivotal role in targeting作者: conifer 時間: 2025-3-30 16:47 作者: 舔食 時間: 2025-3-30 22:09 作者: conjunctiva 時間: 2025-3-31 03:27
Ainor Khaliah Mohd Isa,Nurul Shahira Azuddinisclosing the evolutionary history—.., the phylogeny—of a protein family of interest and then inferring the sequences of its ancestors—.., the nodes in the phylogeny. Assisted by gene synthesis, the selected ancestors can be resurrected in the lab and experimentally characterized. The crucial step t作者: Malleable 時間: 2025-3-31 07:17
Manuel Ferre,Martin Buss,Carlos Balaguerand there is much interest in exploiting their functional and catalytic capabilities within minimal, de novo designed protein scaffolds. Here we describe how, using only natural biosynthetic and post-translational modification pathways, de novo designed soluble and hydrophobic proteins can be equipp作者: 道學氣 時間: 2025-3-31 10:59
V. Adhikar,A. Karmakar,B. Biswas,C. Saharehension of the thermal stability regulating factors of proteins is needful for the engineering of their thermostability. Protein engineering aims to overcome their natural limitations in tough conditions by refining protein stability and activity. Rational-design approach requires a crystal struct作者: Inflamed 時間: 2025-3-31 14:41 作者: Dissonance 時間: 2025-3-31 19:06
