標(biāo)題: Titlebook: Environmental Monitoring of Bacteria; Clive Edwards Book 1999 Humana Press 1999 [打印本頁] 作者: 嚴(yán)厲 時(shí)間: 2025-3-21 16:20
書目名稱Environmental Monitoring of Bacteria影響因子(影響力)
書目名稱Environmental Monitoring of Bacteria影響因子(影響力)學(xué)科排名
書目名稱Environmental Monitoring of Bacteria網(wǎng)絡(luò)公開度
書目名稱Environmental Monitoring of Bacteria網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Environmental Monitoring of Bacteria被引頻次
書目名稱Environmental Monitoring of Bacteria被引頻次學(xué)科排名
書目名稱Environmental Monitoring of Bacteria年度引用
書目名稱Environmental Monitoring of Bacteria年度引用學(xué)科排名
書目名稱Environmental Monitoring of Bacteria讀者反饋
書目名稱Environmental Monitoring of Bacteria讀者反饋學(xué)科排名
作者: 撫育 時(shí)間: 2025-3-21 20:39
Sampling Sediment and Soil,ing the presence and/or diversity of microorganisms to their function/activity in that environment (.,.). By combining the two approaches this obstacle can be overcome. Paramount in this respect are . sampling procedures that produce samples in a form representative of that environment or habitat (.作者: 無能性 時(shí)間: 2025-3-22 01:26 作者: 贊成你 時(shí)間: 2025-3-22 06:43 作者: 小樣他閑聊 時(shí)間: 2025-3-22 09:20 作者: 碌碌之人 時(shí)間: 2025-3-22 14:51
Magnetic Particle-Based Separation Techniques for Monitoring Bacteria from Natural Environments,les, nontarget cells and biological inhibitors and highly enriched in the target cells or molecules of interest. The processed sample will be ready for the next part of the overall experimental protocol; e.g., a culture step, or a molecular biological procedure, and greater confidence in a successfu作者: 碌碌之人 時(shí)間: 2025-3-22 18:23 作者: 泛濫 時(shí)間: 2025-3-23 01:03 作者: 露天歷史劇 時(shí)間: 2025-3-23 02:25
Analysis of DNA Sequences,t (.) and mammalian (.,.) tissue and food or water samples (.,.). The techniques most widely used in detection methods are the polymerase chain reaction (PCR) and oligonucleotide probing. PCR exploits primers targeting a region of ribosomal RNA (rRNA) known to be specific to the organism of interest作者: 不能強(qiáng)迫我 時(shí)間: 2025-3-23 06:53
Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes nonrepresentative nature of traditional analytical methods, as a result of their requirement for prior cultivation, have led to the introduction of molecular biological approaches to these areas of study (.). Considerable effort has led to the development and application of a number of molecular pr作者: EXALT 時(shí)間: 2025-3-23 13:28 作者: 個(gè)阿姨勾引你 時(shí)間: 2025-3-23 16:24
Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology, development of molecular biological techniques has facilitated this task, primarily via the cloning and sequencing of microbial genes retrieved from the environment. However, the labor-intensive nature of a cloning procedure, as well as the biases that it can introduce, have generated the need for 作者: 有角 時(shí)間: 2025-3-23 21:38 作者: 骯臟 時(shí)間: 2025-3-23 22:50
Characterizing Microorganisms in the Environment by Fatty Acid Analysis,bial ecologists. There are many reasons why assessment of microbes in the environment is so demanding, not least their number, diversity, and limited size. Unlike eukaryotes, descriptions of the morphology of prokaryotes usually yields little or no information concerning the phylogenetic affiliation作者: Obligatory 時(shí)間: 2025-3-24 03:23 作者: 細(xì)胞 時(shí)間: 2025-3-24 07:52 作者: Nostalgia 時(shí)間: 2025-3-24 13:48
Confocal Laser Scanning Microscopy of Environmental Samples,ic constituents of living organisms, have brought about a revolution in approaches to biological problems. Whereas spatial organisation at an ultrastructural level would have seemed definitely the province of electron microscopy just a few years ago, now the new methods of light microscopy can offer作者: 運(yùn)動(dòng)的我 時(shí)間: 2025-3-24 16:51 作者: resistant 時(shí)間: 2025-3-24 21:45
Experimental Biofilms and Their Applications in the Study of Environmental Processes,tudying planktonic bacteria under in vitro conditions has undoubtedly yielded important data in a wide range of areas; however, the examination of several environmental habitats, extreme or otherwise, such as a drinking water pipeline has revealed only relatively low numbers of planktonic cells. In 作者: 運(yùn)動(dòng)性 時(shí)間: 2025-3-25 01:23
Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes olecular biological approaches to these areas of study (.). Considerable effort has led to the development and application of a number of molecular procedures to profile the diversity of microbial sequences in environments.作者: anachronistic 時(shí)間: 2025-3-25 03:20
1940-6061 lecular methods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations.Many of the methods enable bacteria to 作者: MODE 時(shí)間: 2025-3-25 11:17 作者: 擋泥板 時(shí)間: 2025-3-25 12:36
A Science Comic of Urban Metro Structure(.). By contrast, newer methods based on the use of molecular biology methods to analyze total extracted DNA from natural specimens, potentially sample the entire population and, thus, provide a better representative picture of the total microbial community.作者: 冰雹 時(shí)間: 2025-3-25 17:49 作者: Enliven 時(shí)間: 2025-3-25 20:37 作者: 嫻熟 時(shí)間: 2025-3-26 00:20
,Systematic Errors and θ13 Sensitivity,sruption to the environment being studied. The principles of membrane inlet mass spectrometry (MIMS) have been described elsewhere (.,.), and are summarized in detail here. Although MIMS allows measurements of numerous gases to be made in both the liquid and gas phases, only liquid phase measurements are detailed in this example.作者: glisten 時(shí)間: 2025-3-26 08:20 作者: 擴(kuò)音器 時(shí)間: 2025-3-26 11:39
Automated Sequencing of DNA Retrieved from Environmental Samples,hniques, which greatly reduce the processing time of retrieved DNA molecules and allow the analysis of a sufficient number of clones to make findings more representative of the diversity present within a system. This chapter covers the principles and use of automated DNA sequencing systems.作者: 人造 時(shí)間: 2025-3-26 14:58 作者: considerable 時(shí)間: 2025-3-26 18:30 作者: TOXIN 時(shí)間: 2025-3-27 00:26
https://doi.org/10.1057/9780230288119niques are time consuming, labor-intensive, and give results that are only of retrospective value. For example, a typical pathogen test (e.g., .) can include pre-enrichment, selective enrichment, selective plating, and confirmation can take up to 7 d to complete.作者: 不能約 時(shí)間: 2025-3-27 04:19
New Schools and Educated People,thout a culture step. A further problem of the study of naturally occurring bacteria is the numbers of cells involved. Bacterial populations consist of immense numbers, and finding specific cells against a background that may consist of billions of nontarget cells is time-consuming and difficult.作者: GORGE 時(shí)間: 2025-3-27 08:25 作者: 沙文主義 時(shí)間: 2025-3-27 10:45 作者: 燈泡 時(shí)間: 2025-3-27 15:46 作者: 同音 時(shí)間: 2025-3-27 21:30
Recovery and Analysis of Ribosomal RNA Sequences from the Environment,ips of the uncultured taxa to be determined (.,.). The universally distributed genes most commonly used for such analyses are the rRNA genes, particularly those encoding the small ribosomal subunit RNAs (16S and 18S rRNA). rRNA genes have many advantages over other candidate genes, including the following:作者: Derogate 時(shí)間: 2025-3-28 01:09
Book 1999thods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations.Many of the methods enable bacteria to be monitor作者: urethritis 時(shí)間: 2025-3-28 03:09
Book 1999research in recent years that has challenged some long-held principles of traditional microbiology, Environmental Monitoring of Bacteriagives timely and practical access to powerfultechniques that allow the study of bacterial ecology as communities, as single cells, or at the molecular level. . ....作者: micronutrients 時(shí)間: 2025-3-28 07:02
Some Problems Posed by Natural Environments for Monitoring Microorganisms,. Unfortunately, it is often the case that experimental protocols developed for model species are not transposable to other bacteria. This gulf is often most apparent when deductions made from organisms grown as pure cultures in the laboratory are assumed to apply to those occurring in natural envir作者: Incise 時(shí)間: 2025-3-28 12:31 作者: 黃瓜 時(shí)間: 2025-3-28 17:28 作者: 消滅 時(shí)間: 2025-3-28 18:56
Analysis of DNA Sequences,ce of target organisms. In addition, fluorescently labeled olignucleotides are increasingly being used in combination with flow cytometry for cell counting or sorting (.,.), and with confocal laser scanning microscopy (.,.) to generate . data revealing close spatial associations of organisms in envi作者: 旋轉(zhuǎn)一周 時(shí)間: 2025-3-28 23:48
Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology,ariation in the targeted nucleotide sequences. Nucleotide pair dissociation is mediated in denaturing gradient gels (DGGE) by the chemicals urea and formamide. These denaturants are incorporated into the gel in increasing concentrations to form the denaturing gradient. A so-called melting domain wit作者: antiquated 時(shí)間: 2025-3-29 05:53
Reporter Gene Expression for Monitoring Microorganisms in the Environment,ular organism or to monitor its activity. The reporter gene can be located on a plasmid or to increase the stability of the gene within the cell it can be incorporated into the bacterial chromosome. There are a wide variety of reporter genes available, and all have particular properties that make th作者: 難聽的聲音 時(shí)間: 2025-3-29 08:06 作者: 災(zāi)難 時(shí)間: 2025-3-29 14:04
Fluorescent , Hybridization and the Analysis of the Single Cell,ered as mosaics of highly conserved and highly variable sequence. Regions of highly conserved sequence have remained virtually unchanged throughout evolution and provide ideal targets for so-called universal or consensus probes and for probes directed at higher levels of taxonomic rank. The variable作者: 繁榮中國(guó) 時(shí)間: 2025-3-29 19:23 作者: Chemotherapy 時(shí)間: 2025-3-29 21:34 作者: coddle 時(shí)間: 2025-3-30 02:27 作者: Overstate 時(shí)間: 2025-3-30 04:53
https://doi.org/10.1057/9780230504837. Unfortunately, it is often the case that experimental protocols developed for model species are not transposable to other bacteria. This gulf is often most apparent when deductions made from organisms grown as pure cultures in the laboratory are assumed to apply to those occurring in natural envir作者: 失望未來 時(shí)間: 2025-3-30 11:42 作者: vasculitis 時(shí)間: 2025-3-30 15:08
https://doi.org/10.1007/978-3-642-11196-9lecule of interest. Methods for intact cell separations include flow cytometric cell sorting (.), optical trapping (.), micromanipulation (.), dielectrophoresis (.), ultrasound sedimentation (.), sedimentation field-flow fractionation (.) and elutriation (.,.). The general applicability and the degr作者: Ornithologist 時(shí)間: 2025-3-30 19:46 作者: 同來核對(duì) 時(shí)間: 2025-3-30 23:15
https://doi.org/10.1007/978-1-4419-9788-3ariation in the targeted nucleotide sequences. Nucleotide pair dissociation is mediated in denaturing gradient gels (DGGE) by the chemicals urea and formamide. These denaturants are incorporated into the gel in increasing concentrations to form the denaturing gradient. A so-called melting domain wit作者: 尊嚴(yán) 時(shí)間: 2025-3-31 01:23 作者: 范例 時(shí)間: 2025-3-31 07:17 作者: Occupation 時(shí)間: 2025-3-31 12:23
The Performance of Exclusive Economic Zonesered as mosaics of highly conserved and highly variable sequence. Regions of highly conserved sequence have remained virtually unchanged throughout evolution and provide ideal targets for so-called universal or consensus probes and for probes directed at higher levels of taxonomic rank. The variable作者: ascetic 時(shí)間: 2025-3-31 14:15 作者: 嚴(yán)重傷害 時(shí)間: 2025-3-31 19:03 作者: 辯論的終結(jié) 時(shí)間: 2025-4-1 00:11 作者: 不可磨滅 時(shí)間: 2025-4-1 03:39
https://doi.org/10.1057/9780230504837wth, and maintenance of microorganisms in the laboratory as pure cultures to enable subsequent biochemical and genetic analyses. This approach has been extremely successful for the manipulation and exploitation of microorganisms in terms of disease control and eradication, development of biotechnolo
