標(biāo)題: Titlebook: Enhancers and Promoters; Methods and Protocol Tilman Borggrefe,Benedetto Daniele Giaimo Book 2021 The Editor(s) (if applicable) and The Aut [打印本頁] 作者: CHARY 時(shí)間: 2025-3-21 16:17
書目名稱Enhancers and Promoters影響因子(影響力)
書目名稱Enhancers and Promoters影響因子(影響力)學(xué)科排名
書目名稱Enhancers and Promoters網(wǎng)絡(luò)公開度
書目名稱Enhancers and Promoters網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Enhancers and Promoters被引頻次
書目名稱Enhancers and Promoters被引頻次學(xué)科排名
書目名稱Enhancers and Promoters年度引用
書目名稱Enhancers and Promoters年度引用學(xué)科排名
書目名稱Enhancers and Promoters讀者反饋
書目名稱Enhancers and Promoters讀者反饋學(xué)科排名
作者: cochlea 時(shí)間: 2025-3-21 21:00
Global Run-on Sequencing (GRO-Seq) RNA, such as mRNAs, rarely reflect accurately the rate of in situ transcription in nuclei by RNA polymerases (RNAPs). The “Global Run-on Sequencing (GRO-Seq)” method, developed in 2008, combines the nuclear run-on assay with next-generation deep sequencing to detect nascent RNA levels to annotate t作者: 掙扎 時(shí)間: 2025-3-22 01:08 作者: 你不公正 時(shí)間: 2025-3-22 05:16 作者: Motilin 時(shí)間: 2025-3-22 11:11 作者: Hyaluronic-Acid 時(shí)間: 2025-3-22 15:46 作者: Hyaluronic-Acid 時(shí)間: 2025-3-22 17:59 作者: inspired 時(shí)間: 2025-3-22 22:27
Low Input Targeted Chromatin Capture (Low-T2C)ed with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinf作者: 膠水 時(shí)間: 2025-3-23 03:02 作者: Seminar 時(shí)間: 2025-3-23 06:52
Analysis of Enhancer–Promoter Interactions using CAGE and RADICL-Seq Technologiesrch. Enhancer–promoter interactions are believed to mediate activation of target genes. Bidirectional transcription represents one hallmark of active enhancers that can be measured using transcriptome technologies such as Cap analysis of gene expression (CAGE). Recently, we have developed RNA and DN作者: COMA 時(shí)間: 2025-3-23 12:29 作者: 祖先 時(shí)間: 2025-3-23 15:39
ative ,matin ,roteomics (N-ChroP) to Characterize Histone Post-translational Modification (PTM) ComChIP) protocol with the mass spectrometry (MS) analysis of immunoprecipitated proteins to study the combinatorial enrichment or exclusion of histone post-translational modifications (PTMs) at specific genomic regions, such as promoters or enhancers. We describe the protocol steps from the digestion 作者: VEIL 時(shí)間: 2025-3-23 19:58
Book 2021ers and Promoters: Methods and Protocols.?is a useful guide for future experiments..Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.作者: Figate 時(shí)間: 2025-3-24 02:01 作者: 花費(fèi) 時(shí)間: 2025-3-24 05:01 作者: faction 時(shí)間: 2025-3-24 10:03 作者: 善辯 時(shí)間: 2025-3-24 14:12
Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Captureparticularly suited to genome-wide studies of promoter interactions with distal regulatory elements, such as enhancers. We present the principles and methods for Promoter Capture Hi-C (PCHi-C), from experimental design to data analysis.作者: insolence 時(shí)間: 2025-3-24 16:15
The Physical Chemistry of MEMBRANESA interacting complexes ligated and sequenced (RADICL-Seq) a novel methodology to map genome-wide RNA–chromatin interactions in intact nuclei. Here, we describe how CAGE and RADICL-Seq data can be used to characterize enhancer elements and identify their target genes.作者: Rankle 時(shí)間: 2025-3-24 19:51 作者: 不出名 時(shí)間: 2025-3-25 01:12
Book 2021scribe?enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays.?Written in the .Methods in Molecular Biology?.series format, chapters include comprehensive introductions, lists of the ne作者: optional 時(shí)間: 2025-3-25 06:29 作者: 移植 時(shí)間: 2025-3-25 09:26
The Physical Chemistry of Aqueous Systemsanscription factors (TFs) or histone marks of interest, the method achieves targeted interrogation of chromatin organization at a subset of genomic regions. PLAC-Seq is able to identify long-range chromatin interactions at kilobase-scale resolution with significantly reduced sequencing cost.作者: Pituitary-Gland 時(shí)間: 2025-3-25 15:08
https://doi.org/10.1007/978-3-319-63847-8particularly suited to genome-wide studies of promoter interactions with distal regulatory elements, such as enhancers. We present the principles and methods for Promoter Capture Hi-C (PCHi-C), from experimental design to data analysis.作者: SNEER 時(shí)間: 2025-3-25 18:36
The Physical Basis of Biochemistryascent RNA into a sequencing library followed by next-generation sequencing and computational data analysis. The protocol includes quality control measurements to monitor the success of the main steps. Following this protocol, a NET-Seq library is obtained within 5?days.作者: 腐敗 時(shí)間: 2025-3-25 20:06
The Physical Basis of Electronicspening at promoters or enhancers, nucleosome displacement, or labile nucleosome occupancy depending on the digestion condition used. As presented, MNase-Seq is a versatile tool for investigating chromatin dynamics, regulation, and to define open chromatin regions of regulatory elements in mammalian genomes.作者: Calibrate 時(shí)間: 2025-3-26 01:03 作者: invert 時(shí)間: 2025-3-26 05:38
The Physical Basis of The Direction of Time population of heterotypic H2A.Z–H2A containing nucleosomes. This protocol can be adopted to investigate any pair-wise combination of any histone variant, histone posttranslational modification, or any other protein that binds to a modified nucleosome.作者: 錫箔紙 時(shí)間: 2025-3-26 08:28
Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequeascent RNA into a sequencing library followed by next-generation sequencing and computational data analysis. The protocol includes quality control measurements to monitor the success of the main steps. Following this protocol, a NET-Seq library is obtained within 5?days.作者: CLAM 時(shí)間: 2025-3-26 15:21
Analyses of Promoter , Enhancer, and Nucleosome Organization in Mammalian Cells by MNase-Seqpening at promoters or enhancers, nucleosome displacement, or labile nucleosome occupancy depending on the digestion condition used. As presented, MNase-Seq is a versatile tool for investigating chromatin dynamics, regulation, and to define open chromatin regions of regulatory elements in mammalian genomes.作者: Electrolysis 時(shí)間: 2025-3-26 17:38 作者: Amendment 時(shí)間: 2025-3-26 21:49
Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes population of heterotypic H2A.Z–H2A containing nucleosomes. This protocol can be adopted to investigate any pair-wise combination of any histone variant, histone posttranslational modification, or any other protein that binds to a modified nucleosome.作者: CALL 時(shí)間: 2025-3-27 04:59
Analysis of Enhancer–Promoter Interactions using CAGE and RADICL-Seq TechnologiesA interacting complexes ligated and sequenced (RADICL-Seq) a novel methodology to map genome-wide RNA–chromatin interactions in intact nuclei. Here, we describe how CAGE and RADICL-Seq data can be used to characterize enhancer elements and identify their target genes.作者: hypnogram 時(shí)間: 2025-3-27 06:25 作者: 雪上輕舟飛過 時(shí)間: 2025-3-27 10:47
978-1-0716-1599-7The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 聰明 時(shí)間: 2025-3-27 17:38 作者: 可轉(zhuǎn)變 時(shí)間: 2025-3-27 17:51 作者: Ascribe 時(shí)間: 2025-3-27 21:56 作者: adumbrate 時(shí)間: 2025-3-28 05:35
The Physical Basis of Biochemistryntrol, methods are required that track genome transcription with high precision in vivo. Here, we provide step-by-step guidance for performing native elongating transcript sequencing (NET-Seq) in mammalian cells. NET-Seq allows quantitative measurements of transcription genome-wide, including enhanc作者: 裂隙 時(shí)間: 2025-3-28 06:25 作者: Irascible 時(shí)間: 2025-3-28 12:47 作者: 黑豹 時(shí)間: 2025-3-28 15:06 作者: 連鎖 時(shí)間: 2025-3-28 21:41
The Physical Basis of The Direction of Timewe describe a ChIP-reChIP protocol to identify a heterotypic (asymmetric) H2A.Z–H2A-containing nucleosome. In this method, following MNase digestion of chromatin to mostly a mononucleosome fraction, H2A.Z-containing nucleosomes are first immunoprecipitated using affinity purified anti-H2A.Z antibodi作者: Awning 時(shí)間: 2025-3-29 02:14 作者: 引導(dǎo) 時(shí)間: 2025-3-29 06:45 作者: 鞭打 時(shí)間: 2025-3-29 07:46
The Physical Chemistry of MEMBRANESrch. Enhancer–promoter interactions are believed to mediate activation of target genes. Bidirectional transcription represents one hallmark of active enhancers that can be measured using transcriptome technologies such as Cap analysis of gene expression (CAGE). Recently, we have developed RNA and DN作者: crumble 時(shí)間: 2025-3-29 12:58 作者: 變形 時(shí)間: 2025-3-29 15:48
Roger G. Lentle,Patrick W.M. JanssenChIP) protocol with the mass spectrometry (MS) analysis of immunoprecipitated proteins to study the combinatorial enrichment or exclusion of histone post-translational modifications (PTMs) at specific genomic regions, such as promoters or enhancers. We describe the protocol steps from the digestion 作者: 能夠支付 時(shí)間: 2025-3-29 20:09
The Physical Basis of the Direction of Timeed with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.作者: Progesterone 時(shí)間: 2025-3-30 01:20
Low Input Targeted Chromatin Capture (Low-T2C)ed with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.作者: Receive 時(shí)間: 2025-3-30 07:15
Tilman Borggrefe,Benedetto Daniele GiaimoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 表狀態(tài) 時(shí)間: 2025-3-30 08:46 作者: 連鎖 時(shí)間: 2025-3-30 14:43
https://doi.org/10.1007/978-1-0716-1597-3inflammation; Myc locus; Histone; eRNAs; T-Cells作者: machination 時(shí)間: 2025-3-30 16:43
The Physical Basis of The Direction of TimeAssay for Transposase-Accessible Chromatin using sequencing (.-.) is a method to investigate the accessibility of chromatin in a genome-wide fashion. In this chapter, we provide a brief history of the chromatin accessibility field followed by a detailed protocol to perform ATAC-Seq assay.
