標(biāo)題: Titlebook: Enhancer RNAs; Methods and Protocol Ulf Andersson ?rom Book 2017 Springer Science+Business Media New York 2017 long ncRNAs.transcription.ge [打印本頁] 作者: Destruct 時間: 2025-3-21 18:13
書目名稱Enhancer RNAs影響因子(影響力)
書目名稱Enhancer RNAs影響因子(影響力)學(xué)科排名
書目名稱Enhancer RNAs網(wǎng)絡(luò)公開度
書目名稱Enhancer RNAs網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Enhancer RNAs被引頻次
書目名稱Enhancer RNAs被引頻次學(xué)科排名
書目名稱Enhancer RNAs年度引用
書目名稱Enhancer RNAs年度引用學(xué)科排名
書目名稱Enhancer RNAs讀者反饋
書目名稱Enhancer RNAs讀者反饋學(xué)科排名
作者: 現(xiàn)任者 時間: 2025-3-21 21:31
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/311270.jpg作者: Bph773 時間: 2025-3-22 02:23 作者: acclimate 時間: 2025-3-22 05:12 作者: Parallel 時間: 2025-3-22 11:49
The philosophy of Jules Lachelierr-derived long noncoding RNAs (long ncRNAs), including enhancer RNAs (eRNAs), are an important component of enhancer function, their expression has not been broadly analyzed at a single cell level via imaging techniques. This protocol describes a method to image eRNA in single cells by in situ hybri作者: 情感脆弱 時間: 2025-3-22 15:41 作者: 情感脆弱 時間: 2025-3-22 20:41
https://doi.org/10.1007/978-1-349-19731-6 This protocol uses a small number of 22–28 nucleotide biotinylated antisense oligonucleotides, complementary to regions of the target RNA that are accessible for hybridization, to purify RNAs from a cross-linked chromatin extract. RNA–chromatin complexes are next immobilized on beads, washed, and s作者: circumvent 時間: 2025-3-23 00:09 作者: travail 時間: 2025-3-23 04:23 作者: Ganglion 時間: 2025-3-23 09:10
Carlos Moruj?o,Samuel Dimas,Susana Relvasction of noncoding RNAs at enhancers, termed eRNAs, correlates with higher expression of genes that the enhancer interacts with; hence, eRNAs provide a new tool to model gene activity in normal and disease tissues. Moreover, this unique class of noncoding RNA has diverse roles in transcriptional reg作者: 彎曲道理 時間: 2025-3-23 11:55 作者: Brittle 時間: 2025-3-23 17:56
or distally located target genes. Enhancers have many features that have been discovered using genomic analyses. Recent studies have shown that active enhancers recruit RNA polymerase II (Pol II) and are transcribed, producing enhancer RNAs (eRNAs). GRO-seq, a method for identifying the location and作者: 人充滿活力 時間: 2025-3-23 21:24 作者: Mumble 時間: 2025-3-23 22:41
https://doi.org/10.1007/978-1-349-12224-0through their interactions with specific cis-acting elements within target RNAs. Here, we describe a novel method to detect protein–mRNA interactions, which combines FLAG-peptide modified, multiply-labeled tetravalent RNA imaging probes (FMTRIPs) with proximity ligation (PLA), and rolling circle amp作者: athlete’s-foot 時間: 2025-3-24 03:02 作者: AMOR 時間: 2025-3-24 07:26
https://doi.org/10.1007/978-1-349-15243-8y used for functional genome studies and is partially replacing classical homologous recombination methods in different aspects. CRISPR/Cas9-derived tools indeed allow the production of a wide-range of engineered mutations: from point mutations to large chromosomal rearrangements such as deletions, 作者: 微粒 時間: 2025-3-24 12:26 作者: Choreography 時間: 2025-3-24 16:33
https://doi.org/10.1007/978-1-4939-4035-6long ncRNAs; transcription; genome-wide; RNA manipulation; siRNA; antisense oligos作者: 終點(diǎn) 時間: 2025-3-24 22:33 作者: ORE 時間: 2025-3-25 02:59 作者: 不朽中國 時間: 2025-3-25 06:20
Book 2017ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring transcription by assessing where transcription starts and generally how it occurs.Written in the highly successful .Methods in Molecular Biology .series format, chapters incl作者: Missile 時間: 2025-3-25 09:40
Deciphering Noncoding RNA and Chromatin Interactions: Multiplex Chromatin Interaction Analysis by Pof chromatin. Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET) is a valuable and powerful technique in molecular biology which allows the study of unbiased, genome-wide de novo chromatin interactions with paired-end tags. Here, we describe the standard version of ChIA-PET and a Multiplex ChIA-PET version.作者: intrigue 時間: 2025-3-25 15:02 作者: 魔鬼在游行 時間: 2025-3-25 18:20 作者: libertine 時間: 2025-3-25 21:27
https://doi.org/10.1007/978-1-349-12224-0lification (RCA). This assay detects native RNA in a sequence specific and single RNA sensitive manner, and PLA allows for the quantification and localization of protein–mRNA interactions with single-interaction sensitivity.作者: 品嘗你的人 時間: 2025-3-26 01:07 作者: Gentry 時間: 2025-3-26 07:32
https://doi.org/10.1007/978-94-017-4740-0is fractionation protocol, which can be readily applied to many mammalian cell types. For the study of long noncoding RNAs and enhancer RNAs in regulation of transcription especially the preparation of chromatin-associated RNA can contribute significantly to further developments.作者: 羽毛長成 時間: 2025-3-26 10:20
The philosophy of Jules Lachelieranscript at the site of transcriptional elongation, thereby permitting analysis of dynamics between the two transcript species in single cells. Our approach is not limited to eRNAs, but can be implemented on other transcripts.作者: accordance 時間: 2025-3-26 15:06
https://doi.org/10.1007/978-1-349-19731-6dentify RNA–chromatin associations on a large scale. CHART-based strategies can be applied to determine the nature and extent of long noncoding RNA (long ncRNA) association with chromatin genome-wide and identify direct long ncRNA transcriptional targets.作者: Assemble 時間: 2025-3-26 17:24 作者: Painstaking 時間: 2025-3-26 23:10 作者: Gorilla 時間: 2025-3-27 03:27 作者: 咒語 時間: 2025-3-27 06:20
Visualization of Enhancer-Derived Noncoding RNA,anscript at the site of transcriptional elongation, thereby permitting analysis of dynamics between the two transcript species in single cells. Our approach is not limited to eRNAs, but can be implemented on other transcripts.作者: guzzle 時間: 2025-3-27 11:31
Mapping Long Noncoding RNA Chromatin Occupancy Using Capture Hybridization Analysis of RNA Targets dentify RNA–chromatin associations on a large scale. CHART-based strategies can be applied to determine the nature and extent of long noncoding RNA (long ncRNA) association with chromatin genome-wide and identify direct long ncRNA transcriptional targets.作者: Outwit 時間: 2025-3-27 16:09
,Detecting Long-Range Enhancer–Promoter Interactions by Quantitative Chromosome Conformation Capturech less frequent. 3C is becoming increasingly widespread in its use for understanding genome organization. Here we provide a protocol for quantitative 3C using real-time PCR analysis, along with essential quality controls and normalization methods.作者: 植物茂盛 時間: 2025-3-27 20:54
Targeted Gene Activation Using RNA-Guided Nucleases,bes key experimental design considerations, methods for plasmid construction, gene delivery protocols, and procedures for analysis of targeted gene activation in mammalian cell lines using CRISPR-Cas transcription factors.作者: glomeruli 時間: 2025-3-28 01:23 作者: 祖?zhèn)?nbsp; 時間: 2025-3-28 04:33 作者: 畢業(yè)典禮 時間: 2025-3-28 09:56 作者: COST 時間: 2025-3-28 11:17 作者: BROW 時間: 2025-3-28 17:44
1064-3745 ation advice from the experts.This volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and explo作者: Essential 時間: 2025-3-28 21:37 作者: ANT 時間: 2025-3-29 02:00
Book 2017and tips on troubleshooting and avoiding known pitfalls..?. .Authoritative and cutting-edge, .Enhancer RNAs: Methods and Protocols. aims to ensure successful results in this rapidly developing field..作者: 臭了生氣 時間: 2025-3-29 06:50 作者: 皮薩 時間: 2025-3-29 10:17
https://doi.org/10.1057/9780230306479ased Peak Identification (DPI) and the PROmiRNA software. We apply both methodologies to the challenging task of identifying primary microRNAs transcript (pri-miRNA) start sites and compare the results.作者: conjunctiva 時間: 2025-3-29 13:31
Identification of Transcribed Enhancers by Genome-Wide Chromatin Immunoprecipitation Sequencing, cloud downstream analysis. Here we present a protocol that combines wet and dry bench methods to accurately identify transcribed enhancers genome-wide as well as an experimental procedure to validate these datasets.作者: PRO 時間: 2025-3-29 19:29 作者: 自由職業(yè)者 時間: 2025-3-29 20:02 作者: ambivalence 時間: 2025-3-30 01:21
Computational Approaches for Mining GRO-Seq Data to Identify and Characterize Active Enhancers,re-processing, alignment, and transcript calling. In addition, we describe protocols and computational pipelines for mining GRO-seq data to identify active enhancers, as well as known transcription factor binding sites that are transcribed. Furthermore, we discuss approaches for integrating GRO-seq-作者: 性冷淡 時間: 2025-3-30 07:04
Evaluating the Stability of mRNAs and Noncoding RNAs,ion of yet uncharacterized regulatory ncRNAs. In this chapter, we discuss the methodologies currently used to estimate RNA decay and outline an experimental protocol for genome-wide estimation of RNA stability of protein-coding and lncRNAs. This protocol details the transcriptional blockage of cultu作者: 愛管閑事 時間: 2025-3-30 09:50
Bioinformatics Pipeline for Transcriptome Sequencing Analysis,作者: bacteria 時間: 2025-3-30 15:02
Erratum to: Bioinformatics Pipeline for Transcriptome Sequencing Analysis,作者: 做事過頭 時間: 2025-3-30 20:20
1064-3745 ucible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?. .Authoritative and cutting-edge, .Enhancer RNAs: Methods and Protocols. aims to ensure successful results in this rapidly developing field..978-1-4939-8160-1978-1-4939-4035-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: aerobic 時間: 2025-3-30 23:19
https://doi.org/10.1007/978-94-010-2025-1ch takes advantage of the fact that UV light will only cross-link proteins and nucleic acids that are directly interacting. This approach can provide key mechanistic insight into the function of these newly identified ncRNAs.作者: 有組織 時間: 2025-3-31 01:58
re-processing, alignment, and transcript calling. In addition, we describe protocols and computational pipelines for mining GRO-seq data to identify active enhancers, as well as known transcription factor binding sites that are transcribed. Furthermore, we discuss approaches for integrating GRO-seq-作者: conference 時間: 2025-3-31 07:52
The Philosophy of Science of A. S. Eddingtonion of yet uncharacterized regulatory ncRNAs. In this chapter, we discuss the methodologies currently used to estimate RNA decay and outline an experimental protocol for genome-wide estimation of RNA stability of protein-coding and lncRNAs. This protocol details the transcriptional blockage of cultu作者: commonsense 時間: 2025-3-31 12:31
Cellular Fractionation and Isolation of Chromatin-Associated RNA,ation of cells prior to RNA isolation thus enables the analysis of distinct steps in the lifetime of individual RNA molecules that would be masked in bulk RNA preparations from whole cells. Here, we describe a simple two-step differential centrifugation protocol for the isolation of cytoplasmic, nuc作者: 中古 時間: 2025-3-31 16:51 作者: 洞察力 時間: 2025-3-31 19:46
Visualization of Enhancer-Derived Noncoding RNA,r-derived long noncoding RNAs (long ncRNAs), including enhancer RNAs (eRNAs), are an important component of enhancer function, their expression has not been broadly analyzed at a single cell level via imaging techniques. This protocol describes a method to image eRNA in single cells by in situ hybri作者: 半球 時間: 2025-4-1 00:44
UV-RNA Immunoprecipitation (UV-RIP) Protocol in Neurons,ted. These nonprotein-coding RNAs (ncRNAs) come in many different forms, and they have been shown to have a variety of functions within the cell, influencing processes such as gene expression, mRNA splicing, and transport, just as a few examples. As we delve deeper into studying their mechanisms of 作者: 不整齊 時間: 2025-4-1 04:01 作者: HEDGE 時間: 2025-4-1 08:49 作者: Surgeon 時間: 2025-4-1 11:29
