標(biāo)題: Titlebook: Engineering Natural Product Biosynthesis; Methods and Protocol Elizabeth Skellam Book 2022 The Editor(s) (if applicable) and The Author(s), [打印本頁] 作者: Coenzyme 時間: 2025-3-21 19:32
書目名稱Engineering Natural Product Biosynthesis影響因子(影響力)
作者: 學(xué)術(shù)討論會 時間: 2025-3-21 21:46 作者: octogenarian 時間: 2025-3-22 02:42
Cyanobacterial Genome Sequencing, Annotation, and Bioinformatics,the chapter, we briefly introduce genome mining tools and one successful genome mining example from our laboratory. This chapter provides general guidance regarding the sequencing project and thus includes several references for alternative methods and tools so that the reader can easily modify the 作者: 商品 時間: 2025-3-22 04:53 作者: neurologist 時間: 2025-3-22 12:12
Double Crossover Approach to Inactivate Target Gene in Cyanobacteria,smid, effectively replacing the wild-type gene with the disrupted gene from the plasmid. The . cassette combined with the . gene serve as positive selection to identify double crossover mutants (Cai?and Wolk?(1990), 172(6):3138–3145, J.?Bacteriol). Finally, the functional genes are cloned into anoth作者: Arthritis 時間: 2025-3-22 13:21 作者: Arthritis 時間: 2025-3-22 17:33 作者: 預(yù)定 時間: 2025-3-22 23:21 作者: 造反,叛亂 時間: 2025-3-23 03:31 作者: neoplasm 時間: 2025-3-23 06:37 作者: assent 時間: 2025-3-23 13:15 作者: 單片眼鏡 時間: 2025-3-23 15:51
Fermi surface of hexagonal tungsten carbidesmid, effectively replacing the wild-type gene with the disrupted gene from the plasmid. The . cassette combined with the . gene serve as positive selection to identify double crossover mutants (Cai?and Wolk?(1990), 172(6):3138–3145, J.?Bacteriol). Finally, the functional genes are cloned into anoth作者: 別炫耀 時間: 2025-3-23 19:21 作者: 祖?zhèn)?nbsp; 時間: 2025-3-23 23:31 作者: 一致性 時間: 2025-3-24 03:39 作者: BUST 時間: 2025-3-24 06:31 作者: Mutter 時間: 2025-3-24 13:38
https://doi.org/10.1007/978-1-4684-8947-7 Here, we describe the process of PCR-amplifying fungal gene clusters and re-assembling them in a cloning vector via target-associated recombination in .. The gene cluster-carrying construct is validated and used to transform protoplasts of ., a well-studied host that is able to express the gene clu作者: altruism 時間: 2025-3-24 16:25
Early Evolution of the Atmosphere and Ocean, are often found in fungal species that are genetically intractable or difficult to cultivate, heterologous expression is increasingly being used for compound discovery. In addition, heterologous expression is a useful strategy to elucidate the function of the genes within a BGC and shed light on th作者: UTTER 時間: 2025-3-24 20:59 作者: FOLLY 時間: 2025-3-25 00:34 作者: bypass 時間: 2025-3-25 04:00
The Chemistry of Natural Productssecondary or specialized metabolites. Recently, evolutionary principles have been incorporated into genome mining strategies in an effort to better assess and prioritize novelty and understand their chemical diversification for engineering purposes. Here, we provide an introduction to the principles作者: GULLY 時間: 2025-3-25 07:53 作者: palliative-care 時間: 2025-3-25 12:25
Christa Fest,Karl-Julius SchmidtIn this case, it is notably applied to rationally modify the biosynthetic pathways giving rise to the polyketide natural products, which are heavily exploited in the medical and agricultural arenas. Our aim here is to provide the potential user with a practical guide to exploit this approach for man作者: 下級 時間: 2025-3-25 16:02
Sediment and the phosphate cycle,we report a standard experimental protocol for the deletion of a biosynthetic gene in a . species, using the vector pCRISPomyces-2 developed by Huimin Zhao and collaborators. We also describe how carrying out metabolite analysis can reveal the putative biosynthetic function of the inactivated gene.作者: 共同生活 時間: 2025-3-25 21:33
On Cysteine and Cystine Peptides,products. Due to the sequential biochemistry processed in each domain, the domain architecture of the assembly line enzymes strictly correlates with the product molecule. This colinearity makes assembly line enzymes an ideal target for rational reprogramming. Although many of the past engineering at作者: 廢除 時間: 2025-3-26 01:55 作者: Entropion 時間: 2025-3-26 08:14
Andreas Türler,Kenneth E. Gregorich rich sources of natural bioactive products, including toxic substances, but also because they play an important role in global nitrogen and carbon cycles, and are capable of maintaining versatile environmental niche adaptations. A vast number of cyanobacterial genomes have become available due to f作者: 猜忌 時間: 2025-3-26 08:44 作者: Harbor 時間: 2025-3-26 12:58
Fermi surface of hexagonal tungsten carbidesm, particularly for studying cell differentiation, nitrogen fixation, photosynthesis, production of high-value chemicals, and synthetic biology. Gene knockout is a common approach to assess the function of gene products through assessing phenotypic loss of function. In the method described here, a 作者: 浸軟 時間: 2025-3-26 19:03
Wallace W. Schulz,Robert A. Pennemane clusters (BGCs) that encode cyanobacterial natural products are known, the slow growth and lack of genetic tools in the native producers hampers their modification, characterization, and large-scale production. By engineering heterologous hosts for the expression of cyanobacterial BGCs, sufficient作者: DAUNT 時間: 2025-3-26 22:30 作者: Coordinate 時間: 2025-3-27 03:22
Wolfgang H. Runde,Wallace W. SchulzPNPs are largely unknown. Heterologous pathway reconstitution is a heavily adopted strategy in secondary metabolism characterization. Yeast systems have been broadly utilized in the heterologous production of PNPs and have been considered as a promising platform to investigate plant biosynthetic pat作者: bourgeois 時間: 2025-3-27 06:34
https://doi.org/10.1007/978-1-0716-2273-5Bioactive metabolites; Drug discovery; Transcriptomes; Bioengineering; Non-natural natural products作者: oncologist 時間: 2025-3-27 11:51 作者: Tracheotomy 時間: 2025-3-27 13:36
Adducts and Heterometallic Alkoxides,industries. Recently, heterologous expression has become an irreplaceable technique to functionalize fungal biosynthetic gene clusters and synthesize fungal natural products in various chassis organisms. This chapter describes the general method of using . as a chassis host to investigate fungal biosynthetic pathways.作者: 起波瀾 時間: 2025-3-27 19:16
Sediment and the phosphate cycle,we report a standard experimental protocol for the deletion of a biosynthetic gene in a . species, using the vector pCRISPomyces-2 developed by Huimin Zhao and collaborators. We also describe how carrying out metabolite analysis can reveal the putative biosynthetic function of the inactivated gene.作者: 拋媚眼 時間: 2025-3-27 22:52
Targeted Genetic Engineering via ,-Mediated Transformation in ,box is missing for the investigation of gene function and metabolism. In this chapter, we describe how .-mediated transformation can be used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in ., is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in . f. sp. ..作者: 濕潤 時間: 2025-3-28 02:42
Investigating Fungal Biosynthetic Pathways Using , as a Heterologous Host,industries. Recently, heterologous expression has become an irreplaceable technique to functionalize fungal biosynthetic gene clusters and synthesize fungal natural products in various chassis organisms. This chapter describes the general method of using . as a chassis host to investigate fungal biosynthetic pathways.作者: Kindle 時間: 2025-3-28 07:59 作者: chisel 時間: 2025-3-28 13:47 作者: thrombus 時間: 2025-3-28 15:10 作者: 外露 時間: 2025-3-28 19:27
Elizabeth SkellamIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: garrulous 時間: 2025-3-29 02:30 作者: collagen 時間: 2025-3-29 04:13
A Bioinformatics Workflow for Investigating Fungal Biosynthetic Gene Clusters, of such predictions remains restricted as they provide total numbers of biosynthetic pathways with only very limited biological significance. In this chapter, we describe a workflow to predict and analyze biosynthetic gene clusters in fungal genomes. It relies on similarity networking and phylogeny作者: 自傳 時間: 2025-3-29 09:45 作者: phase-2-enzyme 時間: 2025-3-29 14:16 作者: 強壯 時間: 2025-3-29 17:49
Heterologous Expression of Fungal Biosynthetic Pathways in , Using Episomal Vectors, are often found in fungal species that are genetically intractable or difficult to cultivate, heterologous expression is increasingly being used for compound discovery. In addition, heterologous expression is a useful strategy to elucidate the function of the genes within a BGC and shed light on th作者: 向外才掩飾 時間: 2025-3-29 20:39 作者: 擴張 時間: 2025-3-30 01:26
Investigating Fungal Biosynthetic Pathways Using , as a Heterologous Host,industries. Recently, heterologous expression has become an irreplaceable technique to functionalize fungal biosynthetic gene clusters and synthesize fungal natural products in various chassis organisms. This chapter describes the general method of using . as a chassis host to investigate fungal bio作者: 稱贊 時間: 2025-3-30 04:15 作者: GEON 時間: 2025-3-30 08:46 作者: IDEAS 時間: 2025-3-30 14:39
Engineering Modular Polyketide Biosynthesis in , Using CRISPR/Cas: A Practical Guide,In this case, it is notably applied to rationally modify the biosynthetic pathways giving rise to the polyketide natural products, which are heavily exploited in the medical and agricultural arenas. Our aim here is to provide the potential user with a practical guide to exploit this approach for man作者: STENT 時間: 2025-3-30 18:19
CRISPR/Cas9-Based Methods for Inactivating Actinobacterial Biosynthetic Genes and Elucidating Functwe report a standard experimental protocol for the deletion of a biosynthetic gene in a . species, using the vector pCRISPomyces-2 developed by Huimin Zhao and collaborators. We also describe how carrying out metabolite analysis can reveal the putative biosynthetic function of the inactivated gene.作者: Bernstein-test 時間: 2025-3-30 21:27
Understanding and Manipulating Assembly Line Biosynthesis by Heterologous Expression in ,products. Due to the sequential biochemistry processed in each domain, the domain architecture of the assembly line enzymes strictly correlates with the product molecule. This colinearity makes assembly line enzymes an ideal target for rational reprogramming. Although many of the past engineering at作者: 出汗 時間: 2025-3-31 02:02
Heterologous Expression, Purification, and Characterization of Type II Polyketide Synthase Acyl Carable cost-effective and sustainable access to a range of pharmaceutically relevant molecules. Progress toward this goal hinges on gaining ample access to materials for in vitro characterizations and structural analysis of the components of these synthases. A central component of PKSs is the acyl car作者: Pert敏捷 時間: 2025-3-31 06:08 作者: 現(xiàn)實 時間: 2025-3-31 09:40 作者: FEAS 時間: 2025-3-31 15:28 作者: 袖章 時間: 2025-3-31 18:40
Expression of Cyanobacterial Biosynthetic Gene Clusters in ,e clusters (BGCs) that encode cyanobacterial natural products are known, the slow growth and lack of genetic tools in the native producers hampers their modification, characterization, and large-scale production. By engineering heterologous hosts for the expression of cyanobacterial BGCs, sufficient作者: 瑣碎 時間: 2025-3-31 22:57 作者: 沒有準(zhǔn)備 時間: 2025-4-1 02:47 作者: expdient 時間: 2025-4-1 07:59 作者: legacy 時間: 2025-4-1 14:01 作者: considerable 時間: 2025-4-1 17:12
Inducing Global Expression of Actinobacterial Biosynthetic Gene Clusters,n be employed to broadly stimulate the expression of biosynthetic gene clusters in . and their relatives, follow the transcription of these genes, and assess the antimicrobial activity of the resulting molecules.作者: languor 時間: 2025-4-1 21:58
Expression of Cyanobacterial Biosynthetic Gene Clusters in , material can be produced for research or industry. Although several hosts have been evaluated for the expression of cyanobacterial natural products, this work details the process of expressing BGCs in . via promoter exchange.
