標題: Titlebook: Engineered Zinc Finger Proteins; Methods and Protocol Joel P. Mackay,David J. Segal Book 2010 Springer Science + Business Media, LLC 2010 A [打印本頁] 作者: 衰退 時間: 2025-3-21 17:08
書目名稱Engineered Zinc Finger Proteins影響因子(影響力)
作者: myelography 時間: 2025-3-21 21:01
Quickoffice and Quickoffice Pro,e display for selecting zinc fingers with desired specificities from such libraries. In this chapter we provide updated, detailed protocols for performing zinc finger selections using the bacterial two-hybrid system.作者: 地名詞典 時間: 2025-3-22 04:26
Touching Your Photos and Videos,table repression of known target genes. We review the literature on targeted DNA methylation and gene silencing, summarize the achievements and the challenges that remain, and discuss technical issues critical for this approach.作者: nitroglycerin 時間: 2025-3-22 05:50
Book 2010teins.In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives.The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and de作者: 沒血色 時間: 2025-3-22 09:09 作者: N斯巴達人 時間: 2025-3-22 15:38
Erica Sadun,Michael Grothaus,Steve Sandeng specificities applied to C.H. zinc finger proteins, based on FoldX, a semiautomatic protein design tool. Given a template structure, this method generates candidate mutants for a given target DNA sequence selected by energetic criteria.作者: N斯巴達人 時間: 2025-3-22 17:09
Taking Your iPhone 4 to the Maxolymerase across the ZFP-binding region. Taking into account some design considerations, this method of inhibiting viral transcription can be applied to other viral infections where viral dsDNA occurs.作者: chuckle 時間: 2025-3-22 22:37 作者: BRAVE 時間: 2025-3-23 02:39 作者: 高度 時間: 2025-3-23 07:15 作者: 無能的人 時間: 2025-3-23 12:13 作者: medium 時間: 2025-3-23 15:47
https://doi.org/10.1007/978-3-662-63453-0re the nuclease is properly bound to the DNA. Here, we describe two different methods to quantify ZFN-associated toxicity: the genotoxicity assay is based on quantification of DSB repair foci induced by ZFNs whereas the cytotoxicity is based on assessing cell survival after application of ZFNs.作者: 禍害隱伏 時間: 2025-3-23 21:38 作者: 注入 時間: 2025-3-23 23:48
Quantification of Zinc Finger Nuclease-Associated Toxicityre the nuclease is properly bound to the DNA. Here, we describe two different methods to quantify ZFN-associated toxicity: the genotoxicity assay is based on quantification of DSB repair foci induced by ZFNs whereas the cytotoxicity is based on assessing cell survival after application of ZFNs.作者: Conquest 時間: 2025-3-24 03:04
Generation of Cell-Permeable Artificial Zinc Finger Protein Variantsts by using plasmids or viral vectors. As an alternative delivery method of ZFP variants, we developed cell-permeable ZFP variants by fusing cell-penetrating peptides to ZFP variants. We will describe how to generate cell-permeable artificial ZFP variants and how to examine the cell permeabilities by immunofluorescent staining.作者: 阻礙 時間: 2025-3-24 09:11
Modulation of Gene Expression Using Zinc Finger-Based Artificial Transcription Factorsion molecule and against the promoter of the RNA component of telomerase. Methods to assess DNA binding of the engineered ZFP as well as to determine and improve the cellular effect of ATFs on (endogenous) promoter activity are described.作者: Cytology 時間: 2025-3-24 11:27
In Vitro Assessment of Zinc Finger Nuclease Activityuding the specificity of DNA binding, the kinetics of dimerization of the two ZFN subunits, and the catalytic activity. In order to investigate these parameters individually, a cell-free system that models these reactions is essential. Here, we present a simple and fast method for the functional testing of ZFNs in vitro.作者: 輕打 時間: 2025-3-24 15:51
Engineered Zinc Finger Proteins for Manipulation of the Human Mitochondrial Genomein a sequence-specific manner. Here, basic methods that are helpful in adapting the engineered zinc finger technology for targeting the DNA in mitochondria are presented with the main emphasis on mitochondrial import of zinc finger proteins.作者: Glycogen 時間: 2025-3-24 20:46 作者: negotiable 時間: 2025-3-25 01:22
Silencing of Gene Expression by Targeted DNA Methylation: Concepts and Approachestable repression of known target genes. We review the literature on targeted DNA methylation and gene silencing, summarize the achievements and the challenges that remain, and discuss technical issues critical for this approach.作者: Infect 時間: 2025-3-25 03:52 作者: morale 時間: 2025-3-25 08:59
Preparing Your Media in iTunes,ion molecule and against the promoter of the RNA component of telomerase. Methods to assess DNA binding of the engineered ZFP as well as to determine and improve the cellular effect of ATFs on (endogenous) promoter activity are described.作者: Licentious 時間: 2025-3-25 15:05 作者: Working-Memory 時間: 2025-3-25 19:07
Leitlinien zur Verwundetenversorgungin a sequence-specific manner. Here, basic methods that are helpful in adapting the engineered zinc finger technology for targeting the DNA in mitochondria are presented with the main emphasis on mitochondrial import of zinc finger proteins.作者: Charitable 時間: 2025-3-25 22:47
https://doi.org/10.1007/978-1-60761-753-2Artificial transcription factors; DNA; DNA binding domains; Designer ZFPs; ZFP construction; Zinc finger 作者: Virtues 時間: 2025-3-26 02:26 作者: 歡笑 時間: 2025-3-26 05:39 作者: Incise 時間: 2025-3-26 12:29 作者: 性別 時間: 2025-3-26 13:43
Leitlinien zur Verwundetenversorgunge endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ability of the Surveyor nuclease to selectively cleave distorted duplex DNA formed via cross-annealing of mutated and wild-type sequence.作者: 嚴厲譴責 時間: 2025-3-26 17:51 作者: 睨視 時間: 2025-3-27 01:03 作者: maladorit 時間: 2025-3-27 04:59
,Harnessing “the Cloud” on Your Tablet,roteins. As such, MA has enabled a vast exploration of sequence-specific methods and reagents, ushering in the modern era of zinc finger-based applications that are described in this volume. The first zinc finger nuclease to cleave an endogenous site was created using MA, as was the first artificial作者: Chivalrous 時間: 2025-3-27 07:32 作者: 附錄 時間: 2025-3-27 11:56
Controlling Your Mac: Launchpad,ement of gene expression as well as targeting specific sites on DNA for methylation, recombination, and cleavage. To effectively and quickly conduct selections that consider the synergistic mode of DNA binding by zinc fingers, Isalan and Choo in Aaron Klug’s lab devised a bipartite phage display app作者: countenance 時間: 2025-3-27 16:04 作者: 不能妥協(xié) 時間: 2025-3-27 19:26
Taking Your iPad to the Max, iOS 5 Editionro and in vivo. Conjugation of other functional domains such as transcriptional regulatory domains and endonucleases to ZFPs provided powerful molecular tools to modulate endogenous gene expression and genetic information. These ZFP variants have been introduced into cells as DNA-encoding ZFP varian作者: Antagonism 時間: 2025-3-27 23:04 作者: 組成 時間: 2025-3-28 03:13
Preparing Your Media in iTunes,rotein (ZFP). Depending on the effector domain, ATFs can up- or downregulate gene expression and thus represent powerful tools in biomedical research and allow novel approaches in clinical practice. Here, we describe the construction of ATFs directed against the promoter of the epithelial cell adhes作者: expound 時間: 2025-3-28 06:15
Touching Your Photos and Videosfinger zinc finger proteins (ZFPs) with distinct DNA-binding specificities, each of which is fused to a transcriptional activation or repression domain. Individual zinc fingers (ZFs) recognize their target DNA subsites and retain their binding specificities in the context of artificially constructed作者: Congeal 時間: 2025-3-28 13:13
Touching Your Photos and Videos,ns of this concept include cancer treatment, treatment of viral infections and, in general, treatment of any disease that could be attenuated by the stable repression of known target genes. We review the literature on targeted DNA methylation and gene silencing, summarize the achievements and the ch作者: 貴族 時間: 2025-3-28 17:19
https://doi.org/10.1007/978-1-4302-0642-2ts are able to relax this structure, facilitating the access of transcription factors into the DNA. Herein, we describe a new method, which combines artificial transcription factors (ATFs) and chromatin-remodeling agents to specifically reactivate silenced regions of the genome and reprogram cellula作者: 鋼筆記下懲罰 時間: 2025-3-28 20:12 作者: 同義聯(lián)想法 時間: 2025-3-29 02:57 作者: Aboveboard 時間: 2025-3-29 03:51
https://doi.org/10.1007/978-3-030-31953-3enome engineering arena, including the therapeutic correction of mutated genes in the human genome. Gene editing frequencies of up to 50% in human cells under non-selective conditions reveal the power of the ZFN technology. Activity and toxicity of ZFNs are determined by a number of parameters, incl作者: 燒瓶 時間: 2025-3-29 10:15 作者: 思考才皺眉 時間: 2025-3-29 13:21
Leitlinien zur Verwundetenversorgunge endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ability of the Surveyor nuclease to selectively cleave distorted duplex DNA formed via cross-annealing of mutat作者: RACE 時間: 2025-3-29 19:08
Leitlinien zur Verwundetenversorgungility of such methods would be of great value for basic mitochondrial research and would aid in development of therapies for diseases linked with mutations in mtDNA. Engineered zinc finger proteins have been used as powerful tools for intervening in nuclear gene expression and modifying nuclear DNA 作者: Left-Atrium 時間: 2025-3-29 20:17 作者: 細胞膜 時間: 2025-3-30 01:16 作者: 骨 時間: 2025-3-30 06:08 作者: arrogant 時間: 2025-3-30 09:21 作者: 磨碎 時間: 2025-3-30 15:39 作者: 可用 時間: 2025-3-30 16:49 作者: Arbitrary 時間: 2025-3-30 21:10
Touching Your Photos and Videos library enter cells randomly at a ratio of ~1 per cell. As a result, individual cells express different ZF-TFs and thus display distinct phenotypical changes. Using an appropriate screening or selection method, one can isolate clonal cells that display phenotypes of interest. One can then identify 作者: 種植,培養(yǎng) 時間: 2025-3-31 04:08
