作者: Notorious 時(shí)間: 2025-3-21 23:07 作者: GEN 時(shí)間: 2025-3-22 03:07
,Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS,ion Monitoring or Multi Reaction Monitoring acquisition mode..2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid, which plays a major role in a wide variety of physiological and pathological processes. Analysis of 2-AG by means of LC-MS/MS allows the detection of very low concentrati作者: 帶來的感覺 時(shí)間: 2025-3-22 07:58 作者: 是限制 時(shí)間: 2025-3-22 11:58
Assay of CB1 Receptor Binding,both central nervous system and peripheral tissues. Today, its biochemical identification and pharmacological characterization, as well as the screening of thousands of novel ligands that might be useful for developing CB.-based therapies, are the subject of intense research. Among available techniq作者: 平淡而無味 時(shí)間: 2025-3-22 15:29 作者: 平淡而無味 時(shí)間: 2025-3-22 19:05 作者: 故意釣到白楊 時(shí)間: 2025-3-22 23:58 作者: nurture 時(shí)間: 2025-3-23 03:01 作者: 嚴(yán)重傷害 時(shí)間: 2025-3-23 05:35 作者: 充滿人 時(shí)間: 2025-3-23 11:07
Assay of NAT Activity, chain is transferred from the .-1 position of the donor phospholipid, such as phosphatidylcholine, to the amino group of phosphatidylethanolamine, resulting in the formation of .-acylphosphatidylethanolamine. NAT has long been known to be stimulated by Ca., and hence it has been referred to as Ca.-作者: Dealing 時(shí)間: 2025-3-23 15:40 作者: nostrum 時(shí)間: 2025-3-23 21:05 作者: Radiation 時(shí)間: 2025-3-23 22:46 作者: 惡臭 時(shí)間: 2025-3-24 03:20 作者: chassis 時(shí)間: 2025-3-24 10:05
Assay of Monoacylglycerol Lipase Activity,-AG is one of the main endogenous lipid agonists for cannabinoid receptors in the brain and elsewhere in the body. In the central nervous system (CNS), MGL is localized to presynaptic nerve terminals of both excitatory and inhibitory synapses, where it helps control the regulatory actions of 2-AG on作者: IOTA 時(shí)間: 2025-3-24 13:09 作者: BAIT 時(shí)間: 2025-3-24 15:23
A Sensitive and Versatile Fluorescent Activity Assay for ABHD12, enzymes in this large protein class has remained unclear. One recently found mSH is α/β-hydrolase domain containing 12 (ABHD12) enzyme, whose natural substrate in vivo appears to be the lysophospholipid lysophosphatidylserine (LPS). In vitro, ABHD12 together with monoacylglycerol lipase (MAGL) and 作者: 思想 時(shí)間: 2025-3-24 21:09 作者: 諷刺滑稽戲劇 時(shí)間: 2025-3-25 02:14 作者: 和平主義 時(shí)間: 2025-3-25 05:44
978-1-4939-8071-0Springer Science+Business Media New York 2016作者: 小木槌 時(shí)間: 2025-3-25 09:52
Endocannabinoid Signaling978-1-4939-3539-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 領(lǐng)導(dǎo)權(quán) 時(shí)間: 2025-3-25 12:15 作者: Complement 時(shí)間: 2025-3-25 19:37
Is Software Engineering , Engineering?iological conditions. We describe here an analytical protocol for extraction of endocannabinoids, anandamide (AEA) and 2-arachidonoyl glycerol (2-AG), endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic acid (AA) from biological tiss作者: 持續(xù) 時(shí)間: 2025-3-25 23:15 作者: 平項(xiàng)山 時(shí)間: 2025-3-26 01:37 作者: cutlery 時(shí)間: 2025-3-26 07:28 作者: Introvert 時(shí)間: 2025-3-26 10:49 作者: 機(jī)械 時(shí)間: 2025-3-26 14:34 作者: overweight 時(shí)間: 2025-3-26 20:41
Reliability of passive electronic parts,etect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen. SureFire. assay that targets ERK作者: Inveterate 時(shí)間: 2025-3-26 23:20
https://doi.org/10.1007/978-1-4419-5760-3f G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB. receptor.作者: 言外之意 時(shí)間: 2025-3-27 04:35 作者: 接觸 時(shí)間: 2025-3-27 08:48
Molecular Modeling for Reliability Issues, chain is transferred from the .-1 position of the donor phospholipid, such as phosphatidylcholine, to the amino group of phosphatidylethanolamine, resulting in the formation of .-acylphosphatidylethanolamine. NAT has long been known to be stimulated by Ca., and hence it has been referred to as Ca.-作者: insomnia 時(shí)間: 2025-3-27 09:47 作者: 說不出 時(shí)間: 2025-3-27 14:30
NoC Reuse for SoC Modular Testing, the biological effects of this lipid mediator. The final products of this reaction are arachidonic acid and ethanolamine. In the method described herein, FAAH activity is measured through the use of a radioactive substrate by quantification of reaction products, that is, [.C]-ethanolamine from [.C-作者: 危機(jī) 時(shí)間: 2025-3-27 20:29 作者: 商店街 時(shí)間: 2025-3-27 23:54
Reliability, Yield, and Stress Burn-InB.) cannabinoid receptors. It is thought to be produced through the action of .-1 selective diacylglycerol lipase (DAGL) that catalyzes 2-AG biosynthesis from .-2-arachidonate-containing diacylglycerols. Since 2-AG biosynthetic enzymes have been identified only recently, little information on method作者: DNR215 時(shí)間: 2025-3-28 03:10
https://doi.org/10.1007/978-94-009-0757-7-AG is one of the main endogenous lipid agonists for cannabinoid receptors in the brain and elsewhere in the body. In the central nervous system (CNS), MGL is localized to presynaptic nerve terminals of both excitatory and inhibitory synapses, where it helps control the regulatory actions of 2-AG on作者: Epithelium 時(shí)間: 2025-3-28 08:24
Reliability-based Structural Designse (MAGL), the major 2-arachidonoyl glycerol (2-AG) hydrolase in the brain. Here, we describe a sensitive fluorescent assay of ABHD6 activity in a 96-well-plate format that allows parallel testing of inhibitor activities of up to 40 compounds in a single assay. The method utilizes lysates of HEK293 作者: 亂砍 時(shí)間: 2025-3-28 10:28 作者: agnostic 時(shí)間: 2025-3-28 17:46 作者: 使人煩燥 時(shí)間: 2025-3-28 22:49
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/309696.jpg作者: 稱贊 時(shí)間: 2025-3-28 23:10
https://doi.org/10.1007/978-3-540-34282-3s able to bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB. receptor.作者: 單調(diào)女 時(shí)間: 2025-3-29 03:21 作者: 商談 時(shí)間: 2025-3-29 09:27 作者: Hyperalgesia 時(shí)間: 2025-3-29 12:06
NoC Reuse for SoC Modular Testing, the biological effects of this lipid mediator. The final products of this reaction are arachidonic acid and ethanolamine. In the method described herein, FAAH activity is measured through the use of a radioactive substrate by quantification of reaction products, that is, [.C]-ethanolamine from [.C-ethanolamine]-AEA.作者: Adenocarcinoma 時(shí)間: 2025-3-29 16:12
The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells,s able to bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB. receptor.作者: 模仿 時(shí)間: 2025-3-29 21:09
The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells,f G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB. receptor.作者: 拋媚眼 時(shí)間: 2025-3-30 03:47
Assay of NAPE-PLD Activity,(FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).作者: GNAT 時(shí)間: 2025-3-30 05:16 作者: 我邪惡 時(shí)間: 2025-3-30 09:07
https://doi.org/10.1007/978-1-4939-3539-0Cannabis sativa; endocannabinoid system; N-arachidonoylethanolamine; 2-arachidonoylglycerol; membrane an作者: 全等 時(shí)間: 2025-3-30 13:59 作者: 托運(yùn) 時(shí)間: 2025-3-30 19:31 作者: 難解 時(shí)間: 2025-3-30 22:24
Reliability of passive electronic parts,bent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen. SureFire. assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by .-α-lysophosphatidylinositol (LPI) and certain cannabinoids.作者: intrude 時(shí)間: 2025-3-31 03:46
U. Dinesh Kumar,John Crocker,M. El-Haram anti-inflammatory and analgesic agents. Here, we introduce an NAAA assay system, using [.C]palmitoylethanolamide and thin-layer chromatography. The preparation of NAAA enzyme from native and recombinant sources as well as the chemical synthesis of .-[1′-.C]palmitoyl-ethanolamine is also described.作者: bourgeois 時(shí)間: 2025-3-31 05:31 作者: insurrection 時(shí)間: 2025-3-31 10:29 作者: Melanocytes 時(shí)間: 2025-3-31 15:50 作者: finite 時(shí)間: 2025-3-31 18:55 作者: 平項(xiàng)山 時(shí)間: 2025-4-1 01:15
Reliability, Yield, and Stress Burn-Ineoyl[1-.C]-2-arachidonoylglycerol as the substrate is reported. All the steps needed to perform lipid extraction, fractionation by thin-layer chromatography (TLC), and quantification of radiolabeled [.C]-oleic acid via scintillation counting are described in detail.
