標題: Titlebook: Endocannabinoid Signaling; Methods and Protocol Mauro Maccarrone Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), u [打印本頁] 作者: Nonchalant 時間: 2025-3-21 17:07
書目名稱Endocannabinoid Signaling影響因子(影響力)
書目名稱Endocannabinoid Signaling影響因子(影響力)學科排名
書目名稱Endocannabinoid Signaling網絡公開度
書目名稱Endocannabinoid Signaling網絡公開度學科排名
書目名稱Endocannabinoid Signaling被引頻次
書目名稱Endocannabinoid Signaling被引頻次學科排名
書目名稱Endocannabinoid Signaling年度引用
書目名稱Endocannabinoid Signaling年度引用學科排名
書目名稱Endocannabinoid Signaling讀者反饋
書目名稱Endocannabinoid Signaling讀者反饋學科排名
作者: 高深莫測 時間: 2025-3-21 23:53 作者: 推測 時間: 2025-3-22 01:25 作者: 六個才偏離 時間: 2025-3-22 05:40
Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Bhidonoyl glycerol (2-AG), and endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic acid (AA) from biological tissues using liquid–liquid extraction method and simultaneous quantification by liquid chromatography multiple reaction monitoring (LC/MRM).作者: Crepitus 時間: 2025-3-22 12:13 作者: 貨物 時間: 2025-3-22 14:32
Distribution Models and Functions,olize these lipids is presented here, in orderto put in a better perspective, the relevance of methodologies that help to disclose molecular details of eCB signaling in health and disease. Proper methodological approaches form also the basis for a more rationale and effective drug design and therapeutic strategy to combat human disorders.作者: 貨物 時間: 2025-3-22 19:39
https://doi.org/10.1007/978-3-662-05409-3hput radioligand binding assay that allows to evaluate efficacy and potency of different compounds, which might represent the basis for the development of new drugs that target CB.-dependent human diseases.作者: 啞巴 時間: 2025-3-22 23:33
Materials and Device Degradation,of .-arachidonoylethanolamine (AEA) and 2-arachidonoylglycerol (2-AG) in the sites where these major eCBs are indeed needed. This chapter will briefly review some preclinical and clinical evidence for the therapeutic potential of ECS pharmacological manipulation.作者: 疾馳 時間: 2025-3-23 04:58 作者: 慢跑鞋 時間: 2025-3-23 06:15
Assay of CB1 Receptor Binding,hput radioligand binding assay that allows to evaluate efficacy and potency of different compounds, which might represent the basis for the development of new drugs that target CB.-dependent human diseases.作者: 漂泊 時間: 2025-3-23 12:07
Endocannabinoid Metabolism and Transport as Drug Targets,of .-arachidonoylethanolamine (AEA) and 2-arachidonoylglycerol (2-AG) in the sites where these major eCBs are indeed needed. This chapter will briefly review some preclinical and clinical evidence for the therapeutic potential of ECS pharmacological manipulation.作者: implore 時間: 2025-3-23 15:23 作者: 地牢 時間: 2025-3-23 20:54
Reliability Evaluation of Power Systemson proximity assay (SPA) for PPAR α, γ, and δ. These SPAs allow to assess the binding affinities of PPAR α, γ, and δ ligands, along with their respective subtype selectivity profiles. Therefore, SPA is an important tool for hit discovery and lead optimization campaigns aimed at identifying next-generation PPAR ligands.作者: 試驗 時間: 2025-3-24 00:26 作者: Ambiguous 時間: 2025-3-24 06:19
Heat Generation and Dissipation,s a chemiluminescent read-out and can be performed in 384-well plates. We have successfully used this assay to characterize a set of reference ligands (both agonists, antagonists, and an inverse agonist) on human CB.R and CB.R, of which some examples will be presented here.作者: CHARM 時間: 2025-3-24 10:07 作者: PRE 時間: 2025-3-24 14:18 作者: 得意人 時間: 2025-3-24 16:40
Measuring the Content of Endocannabinoid-Like Compounds in Biological Fluids: A Critical Overview ors (i.e., lipo amino acids, lipoamines, .-acyl amides) in biological fluids. Most of our focus will be on the analysis of these lipids in plasma/serum, but we will also discuss how the same techniques can be used for the analysis of saliva and breast milk.作者: 使人煩燥 時間: 2025-3-24 20:14
,Scintillation Proximity Assay (SPA)-Based Radioligand Binding for PPARα, PPARγ, and PPARδ Receptorson proximity assay (SPA) for PPAR α, γ, and δ. These SPAs allow to assess the binding affinities of PPAR α, γ, and δ ligands, along with their respective subtype selectivity profiles. Therefore, SPA is an important tool for hit discovery and lead optimization campaigns aimed at identifying next-generation PPAR ligands.作者: Talkative 時間: 2025-3-25 01:35 作者: 門閂 時間: 2025-3-25 06:00 作者: 群居男女 時間: 2025-3-25 08:55
Assay of NAT Activity, family (also known as HRAS-like suppressor family) show Ca.-independent NAT activity. In this chapter, we describe (1) partial purification of Ca.-dependent NAT from rat brain, (2) purification of recombinant cPLA.ε and PLAAT-2, and (3) NAT assay using radiolabeled substrate.作者: 偶像 時間: 2025-3-25 12:45 作者: BLANC 時間: 2025-3-25 16:34 作者: 惡臭 時間: 2025-3-25 22:19 作者: 異端邪說2 時間: 2025-3-26 01:06
Distribution Models and Functions,e Δ.-tetrahydrocannabinol. During the last 20 years, several .-acylethanolamines and acylesters have been shown to act as eCBs, and a complex array of receptors, metabolic enzymes, and transporters (that altogether form the so-called “eCB system”) has been shown to finely tune their manifold biologi作者: 有害處 時間: 2025-3-26 08:08
https://doi.org/10.1007/978-3-662-54209-5ical conditions. We describe here an analytical protocol for the extraction of endocannabinoids, anandamide (archidonoyl ethanolamide, AEA) and 2-arachidonoyl glycerol (2-AG), and endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic a作者: MEET 時間: 2025-3-26 09:41 作者: 乳白光 時間: 2025-3-26 14:31 作者: 鋸齒狀 時間: 2025-3-26 19:42 作者: LURE 時間: 2025-3-26 23:29
Complex System Reliability Analysis Methods,olecular mechanisms underlying the therapeutic properties of the phytocannabinoids, the active compounds in this plant, occurred in the last years of the past century. In the late 1980s and early 1990s, seminal studies demonstrated the existence of cannabinoid receptors and other elements of the so-作者: MOTIF 時間: 2025-3-27 01:25 作者: membrane 時間: 2025-3-27 07:45 作者: affluent 時間: 2025-3-27 10:03
Application of the binomial distribution,ion of a wide variety of chemical and physical noxious stimuli, ranging from exogenous and endogenous ligands to noxious heat (>42?°C) and low pH (pH?
作者: incisive 時間: 2025-3-27 17:00
Systems with non-exponential distributions,esity, inflammatory-related diseases, and cancer. Therefore, identifying novel drugs that are able to restore PPAR-γ activity is a current challenge, which is however slowed down by the lack of a rapid and reproducible activity assay. To date, only a few methods are able to characterize PPAR-γ activ作者: MOTIF 時間: 2025-3-27 20:48
Reliability Evaluation of Power Systemsrent selectivity for the PPAR α, γ, and δ subtypes have been introduced into the market or have reached advanced clinical trials. Binding assays are of utmost importance for the discovery and profiling of such PPAR ligands. Binding assays are often based on radioligands, in particular, tritiated mol作者: 無可爭辯 時間: 2025-3-28 00:10
https://doi.org/10.1007/978-1-4899-1860-4ysiologic processes, through heterodimerization with retinoid X receptor and complex formation with various cofactors. The specific cofactors recruited to PPAR-RXR complexes in response to different ligands lead to major differences in the transactivation of target genes. We developed a cofactor rec作者: 可商量 時間: 2025-3-28 03:03
Rui Peng,Qingqing Zhai,Jun Yangs) of G-protein coupled receptor ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available nonradioligand ready-to-use kits and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB. receptor.作者: ULCER 時間: 2025-3-28 07:36
Heat Generation and Dissipation,ation by (partial) agonists including the G protein pathway and the recruitment of β-arrestins. Differences in the activation level of these pathways lead to biased signaling. Here, we describe a detailed protocol to characterize the potency and efficacy of ligands to induce or inhibit β-arrestin re作者: 預防注射 時間: 2025-3-28 12:45
Materials and Device Degradation,r the development of novel therapeutic drugs for treating several diseases. However, the need for strategies to circumvent the unwanted psychotropic and immunosuppressive effects associated with cannabinoid receptor agonism/antagonism has led to considerable research in the field of molecular altern作者: 重疊 時間: 2025-3-28 16:15
Statistical Inference from Incomplete Data, chain is transferred from the .-1 position of the donor phospholipid, such as phosphatidylcholine, to the amino group of phosphatidylethanolamine, resulting in the formation of .-acylphosphatidylethanolamine. NAT has long been known to be stimulated by Ca. and hence referred to as Ca.-dependent NAT作者: 來自于 時間: 2025-3-28 19:46
https://doi.org/10.1007/978-981-16-0995-4a family of bioactive lipids including anandamide as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin layer chromatography (TLC).作者: cogent 時間: 2025-3-29 00:57 作者: 是突襲 時間: 2025-3-29 03:41
Mauro MaccarroneIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 共和國 時間: 2025-3-29 08:37
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/309695.jpg作者: Onerous 時間: 2025-3-29 11:43 作者: 沒有貧窮 時間: 2025-3-29 16:37 作者: legacy 時間: 2025-3-29 20:33 作者: sclera 時間: 2025-3-30 01:28 作者: 中止 時間: 2025-3-30 06:21 作者: gait-cycle 時間: 2025-3-30 08:35 作者: Frenetic 時間: 2025-3-30 16:13
Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells,an bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB. receptor.作者: 大量殺死 時間: 2025-3-30 17:09 作者: insular 時間: 2025-3-30 21:36 作者: 并置 時間: 2025-3-31 00:57 作者: 變量 時間: 2025-3-31 06:06
,Assay of GTPγS Binding in Autoradiography,Autoradiography of radiolabeled GTPγS ([.S]GTPγS) binding is a relevant technique to study the function of G protein-coupled receptors (GPCRs) .. Here, we describe the protocol for such a method, suitable for investigating CB. receptor functionality in tissue slices from rodent brains.作者: LIEN 時間: 2025-3-31 10:24
1064-3745 ing: Methods and Protocols, Second Edition .is a valuable resource for all researchers interested in learning more about this important and developing field..978-1-0716-2730-3978-1-0716-2728-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Innocence 時間: 2025-3-31 13:24
Reliability Analysis in the Design Phase,docannabinoids. The chapter contains a step-by-step protocol for the analysis of (n-3) endocannabinoids in plasma, including sample collection and solid phase extraction, LC–MS analysis, and data processing. In addition, protocol modifications are provided to allow quantification of n-3 PUFA-derived作者: 使尷尬 時間: 2025-3-31 18:41
Complex System Reliability Analysis Methods,physiological effects at both central and peripheral levels, which may have therapeutic value in different human pathologies, then providing a solid experimental explanation for both the ancient medicinal uses of Cannabis plant and the recent advances in the development of cannabinoid-based specific作者: decode 時間: 2025-3-31 22:58 作者: ferment 時間: 2025-4-1 02:26 作者: employor 時間: 2025-4-1 09:17
Analysis of Omega-3 Fatty Acid-Derived ,-Acylethanolamines in Biological Matrices,docannabinoids. The chapter contains a step-by-step protocol for the analysis of (n-3) endocannabinoids in plasma, including sample collection and solid phase extraction, LC–MS analysis, and data processing. In addition, protocol modifications are provided to allow quantification of n-3 PUFA-derived作者: 斥責 時間: 2025-4-1 11:57
Endocannabinoid-Binding Receptors as Drug Targets,physiological effects at both central and peripheral levels, which may have therapeutic value in different human pathologies, then providing a solid experimental explanation for both the ancient medicinal uses of Cannabis plant and the recent advances in the development of cannabinoid-based specific作者: 真 時間: 2025-4-1 16:55 作者: 會犯錯誤 時間: 2025-4-1 19:03
,DNA–Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding, its peroxisome proliferator response elements (PPRE) consensus sequence. Thus, DPI-ELISA represents a useful assay for PPAR-γ studies, as well as for the identification of novel PPAR-γ ligands for the development of innovative therapeutic approaches to human diseases where PPAR-γ signaling is dysre作者: dermatomyositis 時間: 2025-4-2 01:05
