作者: 大猩猩 時間: 2025-3-21 23:20 作者: 雪崩 時間: 2025-3-22 02:59
https://doi.org/10.1007/978-1-0716-2437-1Stem cells; Induced pluripotent stem cells; Cell lineage; Organoids; ESCs作者: 制定法律 時間: 2025-3-22 05:43
https://doi.org/10.1007/978-3-476-03665-0 powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.作者: chemoprevention 時間: 2025-3-22 11:06 作者: Aviary 時間: 2025-3-22 16:29
978-1-0716-2439-5The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Aviary 時間: 2025-3-22 20:36
Embryonic Stem Cell Protocols978-1-0716-2437-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 尊敬 時間: 2025-3-23 00:48
https://doi.org/10.1007/978-3-322-83289-4a highly multiplexed manner. A large number of genes are perturbed in a cell population through genomic integration of one single-guide RNA (sgRNA) per cell. A subset of cells with the phenotype of interest can then be enriched through fluorescence-activated cell sorting (FACS). SgRNAs with altered 作者: 鬼魂 時間: 2025-3-23 03:48
https://doi.org/10.1007/978-981-10-8087-6lture is an important milestone for human pluripotent stem cell research which eliminates the feeder cells. Various matrices and medium formulations have been generated for feeder-independent culture. Here we described an mTeSR?1 based feeder-independent human ES cell culture on Matrigel. matrix. Cu作者: Mendicant 時間: 2025-3-23 05:50
ewal and pluripotency. These cells have broad differentiation capability to convert into diverse cell types that make up the primary germ layers during embryonic development. iPS cells can spontaneously differentiate and form cell aggregates termed embryoid bodies (EBs) in the absence of differentia作者: cajole 時間: 2025-3-23 09:50
https://doi.org/10.1007/978-3-476-03665-0 powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.作者: 仔細(xì)檢查 時間: 2025-3-23 17:24 作者: Ordnance 時間: 2025-3-23 19:07 作者: N防腐劑 時間: 2025-3-23 23:42 作者: Ovulation 時間: 2025-3-24 02:27
Anthony A. Peguero,Jun Sung Hongntiation method to generate inner ear organoids containing sensory epithelia with hair cells. Human pluripotent stem cells are aggregated in low-binding 96-well plates and treated in chemically defined media with extracellular matrix to promote epithelialization. Small molecules and recombinant prot作者: 漫步 時間: 2025-3-24 06:33 作者: defeatist 時間: 2025-3-24 14:45 作者: 松緊帶 時間: 2025-3-24 16:10
https://doi.org/10.1007/978-1-84628-660-5used to isolate and quantitatively characterize human and mouse hematopoietic cells, often using fluorescently labeled antibodies. However, such analysis is limited in zebrafish due to lack of antibodies that recognize zebrafish hematopoietic cells. We here describe methods for isolation of hematopo作者: 一回合 時間: 2025-3-24 20:12 作者: Gentry 時間: 2025-3-25 01:50
https://doi.org/10.1007/978-981-10-7467-7derm, and endoderm. Pluripotency is usually demonstrated in vitro by spontaneous differentiation of hESCs grown on a monolayer of feeder-cells using an embryoid bodies (EBs)-based method. However, currently hESCs are grown mostly using fully defined media in the absence of a feeder layer. Here we de作者: 天賦 時間: 2025-3-25 06:32 作者: 詞根詞綴法 時間: 2025-3-25 10:38 作者: white-matter 時間: 2025-3-25 13:16
https://doi.org/10.1007/978-3-8349-3650-9mbryonic stem cells (ESCs), which serves as a useful model for understanding early embryo development. Due to the mixture of different cell types, it is necessary to investigate EBs at the single-cell level. Here, we describe a robust and straightforward method for single-cell gene expression profil作者: anthropologist 時間: 2025-3-25 19:00 作者: Lineage 時間: 2025-3-25 23:25
https://doi.org/10.1007/978-3-658-20614-7tiate into cells of all three germ layers (pluripotency). These unique properties make them exceptionally valuable in basic science and clinical researches, including cell replacement therapies, drug discovery, and regenerative medicine. Mouse ESCs represent an important model system for studying ge作者: ANNUL 時間: 2025-3-26 03:41 作者: CRP743 時間: 2025-3-26 07:42 作者: 中世紀(jì) 時間: 2025-3-26 08:47
Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids,ells, as well as neurons with synaptic formations to hair cells. This human stem cell–derived inner ear organoid system provides an ideal platform to study human inner ear development and disease in vitro.作者: Atrium 時間: 2025-3-26 15:09
Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs), Metabolic Assay. This may be a useful protocol for understanding how the glycolytic function of mESCs changes in certain circumstances and how is it coupled to diverse pluripotency/differentiation phenotypes.作者: connoisseur 時間: 2025-3-26 18:15 作者: Inflated 時間: 2025-3-26 22:13 作者: NORM 時間: 2025-3-27 01:23 作者: SOW 時間: 2025-3-27 06:57
riefly, a specific number of iPS cells are placed in droplets on the lid of culture dishes and incubated for 2?days, yielding embryoid bodies, which are suspended and plated. Spontaneous beating of cardiomyocytes can be seen 7–14?days after the plating of EBs and specific cardiac markers can be obse作者: 注入 時間: 2025-3-27 09:39
https://doi.org/10.1007/978-3-642-94399-7 shape of EBs, and translocation of individual cells and cell layers. This chapter describes a comprehensive framework for HCIA for 3D EB differentiation model that allows investigators to analyze EB growth, differentiation, and morphogenetic dynamics.作者: CLOWN 時間: 2025-3-27 14:58 作者: SOB 時間: 2025-3-27 18:57
https://doi.org/10.1007/978-3-658-28394-0 Metabolic Assay. This may be a useful protocol for understanding how the glycolytic function of mESCs changes in certain circumstances and how is it coupled to diverse pluripotency/differentiation phenotypes.作者: 小卒 時間: 2025-3-28 01:01 作者: 北極熊 時間: 2025-3-28 04:41
https://doi.org/10.1007/978-3-030-02391-1tia nigra pars compacta (SNpc) in a Parkinson’s disease rodent model. Here, we describe cell culture protocols for EB generation from PSCs that show significant in vivo differentiation toward dopaminergic neurons.作者: GUILT 時間: 2025-3-28 07:04
ChIP-qPCR for Polycomb Group Proteins During Neuronal Differentiation of Human Pluripotent Stem Cel作者: 含沙射影 時間: 2025-3-28 10:35 作者: FISC 時間: 2025-3-28 17:14 作者: 雪白 時間: 2025-3-28 18:51 作者: Complement 時間: 2025-3-29 02:47
https://doi.org/10.1007/978-981-10-8087-6ave been generated for feeder-independent culture. Here we described an mTeSR?1 based feeder-independent human ES cell culture on Matrigel. matrix. Culture, freeze/thaw, passaging and initiation of differentiation in suspension culture were described.作者: 使迷惑 時間: 2025-3-29 04:37 作者: 油氈 時間: 2025-3-29 07:52 作者: 隨意 時間: 2025-3-29 12:27 作者: 終止 時間: 2025-3-29 19:11
Book 2022Latest editionve methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their r作者: evanescent 時間: 2025-3-29 21:32
Feeder-Free Human Embryonic Stem Cell Culture Under Defined Culture Conditions,ave been generated for feeder-independent culture. Here we described an mTeSR?1 based feeder-independent human ES cell culture on Matrigel. matrix. Culture, freeze/thaw, passaging and initiation of differentiation in suspension culture were described.作者: fallible 時間: 2025-3-30 01:00 作者: PHAG 時間: 2025-3-30 04:11 作者: 紳士 時間: 2025-3-30 10:22
https://doi.org/10.1007/978-3-322-95197-7S based on good manufacturing practice and cell therapy-grade reagents. We further describe fully defined protocols to terminally differentiate lt-NES toward GABA-ergic, dopaminergic, and motor neurons.作者: 防銹 時間: 2025-3-30 14:25 作者: 改變立場 時間: 2025-3-30 17:03
https://doi.org/10.1007/978-1-4302-0848-8cols to dissociate hiPSC-CMs by using collagenase A&B, Collagenase II, TrypLE, and EDTA and reseeding on various matrix materials including fibronectin, laminin, imatrix, Matrigel, and Geltrex. By the replating methods described here, a single cell or cluster-containing hiPSC-CM cultures can be generated effectively.作者: 網(wǎng)絡(luò)添麻煩 時間: 2025-3-30 21:56 作者: tenosynovitis 時間: 2025-3-31 02:29 作者: 戰(zhàn)役 時間: 2025-3-31 07:01
https://doi.org/10.1007/978-3-8349-3650-9quires standard molecular biology techniques and lab equipment. It allows for accurate 3′ counting of transcript at the single-cell level, which helps reveal cellular identities during EB formation. Combined with perturbation experiments, the method provides an opportunity for mechanistic studies of embryo development at the single-cell level.作者: 小丑 時間: 2025-3-31 10:57 作者: 天空 時間: 2025-3-31 15:51 作者: FEAS 時間: 2025-3-31 17:55
Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol,lished for mESCs. Here we describe the isolation, culture, and preparation feeder cell layers and establishment of feeder-dependent/independent protocol for mESC culture. In addition, basic mESC protocols for culture, storage, and differentiation were described.作者: 障礙 時間: 2025-4-1 01:01
,Replating Protocol for Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes,cols to dissociate hiPSC-CMs by using collagenase A&B, Collagenase II, TrypLE, and EDTA and reseeding on various matrix materials including fibronectin, laminin, imatrix, Matrigel, and Geltrex. By the replating methods described here, a single cell or cluster-containing hiPSC-CM cultures can be generated effectively.作者: 大猩猩 時間: 2025-4-1 05:14
