作者: LINE 時間: 2025-3-21 23:51 作者: Commonplace 時間: 2025-3-22 01:11
https://doi.org/10.1385/1597450375DNA; Promoter; Vivo; gene expression; gene transfer; genes; transgene expression作者: myocardium 時間: 2025-3-22 06:27 作者: 壕溝 時間: 2025-3-22 09:24
https://doi.org/10.1007/978-3-662-42487-2 which to study the signals that regulate differentiation toward a specific cell type. Here, we describe methods for using embryonic stem cells to study the signals that control differentiation into neurectoderm and into vascular cell types, focusing on the Hedgehog signaling pathway.作者: vocation 時間: 2025-3-22 13:13 作者: vocation 時間: 2025-3-22 18:59
The Use of Embryonic Stem Cells to Study Hedgehog Signaling which to study the signals that regulate differentiation toward a specific cell type. Here, we describe methods for using embryonic stem cells to study the signals that control differentiation into neurectoderm and into vascular cell types, focusing on the Hedgehog signaling pathway.作者: 殺人 時間: 2025-3-22 22:14 作者: 敲詐 時間: 2025-3-23 01:22 作者: 不能逃避 時間: 2025-3-23 08:55 作者: Indolent 時間: 2025-3-23 10:00 作者: deceive 時間: 2025-3-23 16:02
https://doi.org/10.1007/978-3-642-50741-0ell established, and many mouse ES cell lines have been created so far. The establishment of rat ES cell lines, however, has proven to be difficult. Most attempts to culture rat ES cell lines and maintain them in an undifferentiated state have failed, so researchers were forced to abandon this syste作者: HALL 時間: 2025-3-23 22:03 作者: Vulnerary 時間: 2025-3-24 01:52 作者: obsession 時間: 2025-3-24 02:38 作者: 殺人 時間: 2025-3-24 08:03 作者: mortgage 時間: 2025-3-24 14:17 作者: PARA 時間: 2025-3-24 16:03
https://doi.org/10.1007/978-3-662-42507-7pes. Activation of the path-way is associated with many aspects of cellular activity, including cell growth and differen-tiation. Activation of hormone-sensitive PLC results in the rapid breakdown of polyphosphoinositides to generate two second messengers: inositol trisphosphate and dia-cylglycerol.作者: Countermand 時間: 2025-3-24 19:19
https://doi.org/10.1007/978-3-662-01988-7 made to define protocols to preferentially direct ES cells toward a defined cell type. To this end, it becomes crucial to characterize the molecular mechanisms as well as the signaling pathways implicated in ES cell differentiation. Findings highlight a key role of ., the founding member of a new c作者: gastritis 時間: 2025-3-25 03:01
https://doi.org/10.1007/978-3-662-42487-2 which to study the signals that regulate differentiation toward a specific cell type. Here, we describe methods for using embryonic stem cells to study the signals that control differentiation into neurectoderm and into vascular cell types, focusing on the Hedgehog signaling pathway.作者: Inclement 時間: 2025-3-25 06:25
https://doi.org/10.1007/978-3-662-24825-6ro differentiation procedures. To facilitate the genetic modification of ES cells, we describe how to analyze the transcriptional activities of different promoter systems. The DNA segments from a number of promoters are subcloned into the multiple cloning sites of the green fluorescent protein (GFP)作者: 不給啤 時間: 2025-3-25 08:16 作者: 健談 時間: 2025-3-25 14:27 作者: exhibit 時間: 2025-3-25 19:10
Mittel- und Langfristige Exportfinanzierungerful approach to target and inhibit gene expression, RNAi, has been applied to ES cells with the goal of teasing out the cascades of gene expression/repression that shape the early embryo. In this chapter, we describe the current understanding of the mechanisms of gene silencing by small hairpin RN作者: 漫不經(jīng)心 時間: 2025-3-25 22:00 作者: 吝嗇性 時間: 2025-3-26 02:50 作者: periodontitis 時間: 2025-3-26 06:39
Derivation and Culture of Mouse Trophoblast Stem Cells In Vitronent of the placenta and of the parietal yolk sac, making a striking contrast with embryonic stem cells, which never contribute to these tissues in chimeras. In this chapter, detailed protocols for the isolation and establishment of TS cell lines from blastocysts and their maintenance are described.作者: MOTTO 時間: 2025-3-26 09:02
Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitrotocompatibility class 1 molecule. Neuronal differentiation is achieved by standard retinoic acid treatment and endothelial differentiation can be reproducibly induced by growth on or within MatrigelS for 14 d. To induce expression of hepatocyte-specific antigens, RESCs were either grown in hepatocyt作者: 哥哥噴涌而出 時間: 2025-3-26 13:25 作者: antipsychotic 時間: 2025-3-26 18:15 作者: 可以任性 時間: 2025-3-26 22:25
1064-3745 he two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.978-1-61737-601-6978-1-59745-037-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 令人作嘔 時間: 2025-3-27 01:28 作者: 可行 時間: 2025-3-27 08:51
https://doi.org/10.1007/978-3-658-11623-1noclonal antibody-1. In addition, the phosphorylation of signal transducers and acti-vators of transcription-3 by LIF, which is sufficient to maintain the undifferentiated state of ES cells, was detected by Western blotting analysis.作者: 夜晚 時間: 2025-3-27 13:28
K?niglichen Aufsichts-Kommissionnent of the placenta and of the parietal yolk sac, making a striking contrast with embryonic stem cells, which never contribute to these tissues in chimeras. In this chapter, detailed protocols for the isolation and establishment of TS cell lines from blastocysts and their maintenance are described.作者: Phagocytes 時間: 2025-3-27 14:56
https://doi.org/10.1007/978-3-642-50741-0tocompatibility class 1 molecule. Neuronal differentiation is achieved by standard retinoic acid treatment and endothelial differentiation can be reproducibly induced by growth on or within MatrigelS for 14 d. To induce expression of hepatocyte-specific antigens, RESCs were either grown in hepatocyt作者: subacute 時間: 2025-3-27 17:54
https://doi.org/10.1007/978-3-662-42507-7g-naling system appears to be constitutively active and essential for normal ES cell prolifera-tion. Here, we describe in detail the materials required and some of techniques involved in studying the phosphoinositide signaling system in mouse ES cells. Furthermore, we describe methods of analyzing t作者: 污穢 時間: 2025-3-28 00:36
https://doi.org/10.1007/978-3-662-42506-0equence comparisons among stem cells, differentiated cells, and tissues. These markers can then be genetically manipulated to understand the molecular basis for stem cell biology to help define how transcriptional mechanisms distinguish ES cells from other, less-pluripotent cell types. SAGE is, thus作者: 喚醒 時間: 2025-3-28 03:04
Book 2006Latest editionly reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation,作者: 異教徒 時間: 2025-3-28 08:32
1064-3745 cols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular作者: 貞潔 時間: 2025-3-28 11:04
https://doi.org/10.1007/978-3-662-11517-6hematopoietic and endothelial cell lineages in the presence of bFGF, stem cell factor, and oncostatin M. Equine ES cells pro-vide a powerful tool for gene targeting and the generation of transgenic clonal offspring.作者: 放肆的你 時間: 2025-3-28 18:23 作者: Ventricle 時間: 2025-3-28 18:51 作者: 澄清 時間: 2025-3-28 23:19
https://doi.org/10.1007/978-3-662-24825-6ed differing promoter activities in transient transfections of ES cells. The judicious selection of the optimum promoter system for specific genetic modification of ES cells will ensure the successful generation of transgenic ES cell lines.作者: myalgia 時間: 2025-3-29 04:48
Mittel- und Langfristige Exportfinanzierungg constructs, transfection of ES cells, and flow sorting of targeted cells is followed by methods for the analysis of phenotype and behavior of targeted cell populations using immunohistochemistry, reverse transcriptase polymerase chain reaction, Western blotting, and scanning electron microscopy.作者: 新星 時間: 2025-3-29 08:44 作者: 救護車 時間: 2025-3-29 12:04 作者: Hectic 時間: 2025-3-29 16:27
Matrix Assembly, Cell Polarization, and Cell Survivalbasement membrane assembly and its signaling consequences, epiblast polarization, apoptosis, and cavitation can be studied using a combination of genetic, biochemical, cell, and molecular biology approaches.作者: Eulogy 時間: 2025-3-29 20:45 作者: AFFIX 時間: 2025-3-30 01:02 作者: Range-Of-Motion 時間: 2025-3-30 07:54 作者: Monotonous 時間: 2025-3-30 12:03
Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4 cells by means of digital differential display of expressed sequence tag databases. Reporter gene and gel mobility shift assays are used to confirm the role of Oct3/4. Identification of novel Oct3/4 targets will facilitate our understanding of pluripotency.作者: 淡紫色花 時間: 2025-3-30 14:19 作者: 高腳酒杯 時間: 2025-3-30 17:12
https://doi.org/10.1007/978-3-662-01988-7l differentiation as embryoid bodies toward either cardiomyocytes or neurons. Although referred to as modulation of Cripto signaling, these methods build the basis for the use of other classes of secreted molecules to control ES cell differentiation.作者: 善于騙人 時間: 2025-3-30 20:50 作者: Ceramic 時間: 2025-3-31 00:56 作者: 職業(yè) 時間: 2025-3-31 08:58
Mittel- und langfristige Exportfinanzierung cells by means of digital differential display of expressed sequence tag databases. Reporter gene and gel mobility shift assays are used to confirm the role of Oct3/4. Identification of novel Oct3/4 targets will facilitate our understanding of pluripotency.作者: defibrillator 時間: 2025-3-31 10:52
Isolation and Differentiation of Medaka Embryonic Stem Cellsd to mammals, in which embryonic stem (ES) cell lines can be derived from midblastula embryos (MBEs). Like mouse ES cells, medaka ES cells most resemble the totipotent embryonic cells at the blastula stage. Medaka ES cells retain a diploid karyotype, pluripotency in vitro, and chimera competence in 作者: 法律的瑕疵 時間: 2025-3-31 14:49 作者: 作嘔 時間: 2025-3-31 18:06
Derivation and Culture of Mouse Trophoblast Stem Cells In Vitrothe inner cell mass, and the other is the trophectoderm. A subset of the trophectoderm maintains a proliferative capacity and forms the extraembryonic ectoderm, the ectoplacental cone, and the secondary giant cells of the early conceptus after implantation. A stem cell population of the trophectoder作者: Corroborate 時間: 2025-3-31 23:07 作者: 遠足 時間: 2025-4-1 03:19