標(biāo)題: Titlebook: Embryo Models In Vitro; Methods and Protocol Magdalena Zernicka-Goetz,Kursad Turksen Book 2024 The Editor(s) (if applicable) and The Author [打印本頁] 作者: 手或腳 時(shí)間: 2025-3-21 18:25
書目名稱Embryo Models In Vitro影響因子(影響力)
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書目名稱Embryo Models In Vitro被引頻次學(xué)科排名
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書目名稱Embryo Models In Vitro年度引用學(xué)科排名
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書目名稱Embryo Models In Vitro讀者反饋學(xué)科排名
作者: 神刊 時(shí)間: 2025-3-21 22:38 作者: 比目魚 時(shí)間: 2025-3-22 04:02
Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction Sysd applications in developmental biology, related developmental disease modeling, and drug discovery. Several cell resources have been utilized, with different efficiencies and methods for generating human blastoids, a structure similar to natural blastocysts. Human EPS cells were reported to contrib作者: Bouquet 時(shí)間: 2025-3-22 07:40 作者: 脊椎動(dòng)物 時(shí)間: 2025-3-22 08:58
Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Techlly gene editing and transcriptional regulation technology. Human trophoblast stem cell (hTSC) is a promising cell model for simulating the development and function of human placental trophoblast cells. Here, we provide an optimized workflow for efficient genome editing and transcriptional activatio作者: arcane 時(shí)間: 2025-3-22 16:56 作者: arcane 時(shí)間: 2025-3-22 19:27 作者: nautical 時(shí)間: 2025-3-23 00:20
,Generation of 3D Trophoblast Organoids from Human Na?ve Pluripotent Stem Cells,ells found within the placenta and comprise a variety of distinct cell types with specialized roles in fetal-maternal communication. Our understanding of human trophoblast development remains limited due to ethical and legal restrictions on accessing first-trimester placental tissues, as well as the作者: 燒瓶 時(shí)間: 2025-3-23 05:00 作者: Dorsal-Kyphosis 時(shí)間: 2025-3-23 08:39 作者: Communicate 時(shí)間: 2025-3-23 13:02
Generation of Stem Cell-Based Mouse Embryo-Like Structures,” ETiX-embryoids are formed from combined embryonic stem cells, trophoblast stem cells, and embryonic stem cells transiently induced to express Gata4. Cells are seeded into AggreWell dishes where they form aggregates that develop to resemble post-implantation mouse embryos following 4?days of cultur作者: 減去 時(shí)間: 2025-3-23 17:53 作者: Dappled 時(shí)間: 2025-3-23 22:01 作者: SPASM 時(shí)間: 2025-3-23 22:20 作者: 心神不寧 時(shí)間: 2025-3-24 06:22
Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos,logical systems via comprehensive delineations of individual cellular states. Single-cell RNA sequencing in particular has served as a powerful tool to the study of the molecular circuitries underlying preimplantation embryonic development in both the mouse and human. Here we describe a method to el作者: arthroscopy 時(shí)間: 2025-3-24 10:31
Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome to capture biological heterogeneity and assess the cellular composition in early embryos. Comparative analysis of the transcriptional landscapes of embryos with single-cell resolution allows us to better understand and improve stem-cell-based embryo models. However, proper comparison between differ作者: 救護(hù)車 時(shí)間: 2025-3-24 10:39
Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL, and perturbed conditions. In this chapter, we provide a detailed guideline for implementing IQCELL from a raw dataset. The steps include processing data, inferring informative genes, inferring gene regulatory network, and simulating the resulted network under normal and perturbed conditions.作者: 使出神 時(shí)間: 2025-3-24 18:54 作者: 菊花 時(shí)間: 2025-3-24 20:15
Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence,nslate findings to human development, it is crucial to select an appropriate animal model that closely resembles the specific aspect of development under study. The guinea pig is highlighted as a useful platform for reproductive studies due to similarities in in utero development and general physiol作者: 強(qiáng)行引入 時(shí)間: 2025-3-25 00:11 作者: 惡意 時(shí)間: 2025-3-25 05:19
Hans-Dieter Barke,Al Hazari,Sileshi Yitbarektablished an extended three-dimensional (3D) culture system of human blastocysts (Xiang et al., Nature 577(7791):537–542, 2020). The 3D embryo culture system could enable human blastocyst growing up to early primitive streak anlage stage in vitro. Here, we introduce the detail protocol and notes of culturing human 3D embryos.作者: 核心 時(shí)間: 2025-3-25 09:14
Misleading Marketing Communicationt and function of human placental trophoblast cells. Here, we provide an optimized workflow for efficient genome editing and transcriptional activation in hTSCs using a lentivirus-dependent method. We also present methods to evaluate the gene targeted mutations and transcriptional activation.作者: 熱情的我 時(shí)間: 2025-3-25 11:48
https://doi.org/10.1007/978-3-663-10811-5 developing embryo. Recently, it has been shown that self-renewing extraembryonic mesoderm cells (EXMCs) can be modeled in vitro by using human naive pluripotent stem cells. Here, we present a detailed step-by-step protocol to induce EXMCs from naive pluripotent stem cells in vitro.作者: aspect 時(shí)間: 2025-3-25 16:06
Human Pre-gastrulation Embryo Culture in 3D Condition,tablished an extended three-dimensional (3D) culture system of human blastocysts (Xiang et al., Nature 577(7791):537–542, 2020). The 3D embryo culture system could enable human blastocyst growing up to early primitive streak anlage stage in vitro. Here, we introduce the detail protocol and notes of culturing human 3D embryos.作者: Valves 時(shí)間: 2025-3-25 21:01 作者: 嗎啡 時(shí)間: 2025-3-26 03:11
Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells, developing embryo. Recently, it has been shown that self-renewing extraembryonic mesoderm cells (EXMCs) can be modeled in vitro by using human naive pluripotent stem cells. Here, we present a detailed step-by-step protocol to induce EXMCs from naive pluripotent stem cells in vitro.作者: interference 時(shí)間: 2025-3-26 04:23
Misinformation and Disinformation and maternal environment. Here, we present a protocol to obtain different types of placental trophoblast cells and trophoblast organoids using hTSCs. The generation of hTSC-organoids takes 6 days. hTSC-organoids permit passaging and can differentiate into EVT lineage.作者: caldron 時(shí)間: 2025-3-26 09:32 作者: intrude 時(shí)間: 2025-3-26 14:37 作者: agitate 時(shí)間: 2025-3-26 18:52
https://doi.org/10.1007/978-1-4039-7854-7ucidate the cellular dynamics of the embryo further by performing both single-cell RNA sequencing (Smart-Seq2) and single-cell small non-coding RNA sequencing (Small-Seq) on the same individual embryonic cell.作者: acrimony 時(shí)間: 2025-3-26 23:32 作者: exorbitant 時(shí)間: 2025-3-27 01:59
Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome is chapter, we focus on the analysis of human blastoids, which model the blastocyst, and their integrative analysis with human embryo datasets and a 2D in vitro early development model system dataset, which models epiblast, extraembryonic mesoderm, and trophoblast cells.作者: Deduct 時(shí)間: 2025-3-27 06:02
Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models, and maternal environment. Here, we present a protocol to obtain different types of placental trophoblast cells and trophoblast organoids using hTSCs. The generation of hTSC-organoids takes 6 days. hTSC-organoids permit passaging and can differentiate into EVT lineage.作者: 粗野 時(shí)間: 2025-3-27 10:26 作者: 寬宏大量 時(shí)間: 2025-3-27 15:13 作者: Horizon 時(shí)間: 2025-3-27 21:35
Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos,ucidate the cellular dynamics of the embryo further by performing both single-cell RNA sequencing (Smart-Seq2) and single-cell small non-coding RNA sequencing (Small-Seq) on the same individual embryonic cell.作者: 設(shè)想 時(shí)間: 2025-3-27 22:10 作者: CLAIM 時(shí)間: 2025-3-28 04:48 作者: 拉開這車床 時(shí)間: 2025-3-28 06:15
https://doi.org/10.1007/978-3-8350-9611-0k, a 3D organoid system called “somitoid” supporting the simultaneous formation of somite-like structures, and another organoid system called “segmentoid” reconstituting in vivo–like hallmarks of somitogenesis. Together, these complementary model systems provide an excellent platform to decode somitogenesis and advance human developmental biology.作者: 我吃花盤旋 時(shí)間: 2025-3-28 10:43
Detlef Angermann,Roland Pail,Urs Hugentoblerflush and smear to confirm successful mating, performing euthanasia of the guinea pig, and flushing in vivo embryos. Once collected, the embryos can be utilized for numerous downstream applications. Here we will cover embryo culturing and processing embryos for immunofluorescence.作者: 柳樹；枯黃 時(shí)間: 2025-3-28 17:56
Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (,PASE),we provide detailed instructions needed to reproduce μPASEs, including the immunofluorescence staining and cell retrieval protocols for characterizing μPASEs obtained under different experimental conditions.作者: 畸形 時(shí)間: 2025-3-28 20:38 作者: 言行自由 時(shí)間: 2025-3-29 01:30
Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence,flush and smear to confirm successful mating, performing euthanasia of the guinea pig, and flushing in vivo embryos. Once collected, the embryos can be utilized for numerous downstream applications. Here we will cover embryo culturing and processing embryos for immunofluorescence.作者: affinity 時(shí)間: 2025-3-29 05:13
Book 2024in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written for the highly successful .Methods in Molecular Bio作者: 叫喊 時(shí)間: 2025-3-29 08:58
978-1-0716-3688-6The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 挖掘 時(shí)間: 2025-3-29 13:51 作者: Affirm 時(shí)間: 2025-3-29 18:35 作者: 土產(chǎn) 時(shí)間: 2025-3-29 23:48 作者: 設(shè)施 時(shí)間: 2025-3-30 00:42
stoids serve as an accessible model for human blastocysts and are amenable for large-scale production. Here, we describe a detailed step-by-step protocol for the robust and high-efficient generation of human blastoids from naive human pluripotent stem cells.作者: Parabola 時(shí)間: 2025-3-30 04:54 作者: 阻撓 時(shí)間: 2025-3-30 11:47
https://doi.org/10.1057/9780230287372d applications in developmental biology, related developmental disease modeling, and drug discovery. Several cell resources have been utilized, with different efficiencies and methods for generating human blastoids, a structure similar to natural blastocysts. Human EPS cells were reported to contrib作者: phytochemicals 時(shí)間: 2025-3-30 13:21 作者: 強(qiáng)制令 時(shí)間: 2025-3-30 19:55 作者: 反復(fù)拉緊 時(shí)間: 2025-3-30 23:28
https://doi.org/10.1007/978-3-663-10808-5in knowledge to in vitro modeling. Previous models have relied on monolayer co-cultures, which do not replicate the complexity of endometrial tissue. Here, we detail the establishment of three-dimensional endometrial assembloids, comprising gland-like epithelial organoids in a stromal matrix. Endome作者: 性學(xué)院 時(shí)間: 2025-3-31 01:41
https://doi.org/10.1057/9780230212800r and morphological features resembling the progressive development of the early post-implantation human embryonic sac. The microfluidic device is specifically designed to control the formation of hPSC clusters and expose the clusters to different morphogen environments, allowing the development of 作者: 苦惱 時(shí)間: 2025-3-31 05:48
https://doi.org/10.1007/978-3-662-28405-6ells found within the placenta and comprise a variety of distinct cell types with specialized roles in fetal-maternal communication. Our understanding of human trophoblast development remains limited due to ethical and legal restrictions on accessing first-trimester placental tissues, as well as the作者: COWER 時(shí)間: 2025-3-31 13:07
https://doi.org/10.1007/978-3-663-10811-5ot in rodents. EXM is mesenchymal and plays an important role in embryogenesis, including early erythropoiesis, and provides mechanical support to the developing embryo. Recently, it has been shown that self-renewing extraembryonic mesoderm cells (EXMCs) can be modeled in vitro by using human naive 作者: 我邪惡 時(shí)間: 2025-3-31 15:57
https://doi.org/10.1007/978-3-8350-9611-0ody plan. Somites are sequentially formed from head to tail in a rhythmic manner controlled by an oscillating gene regulatory network known as the segmentation clock. We know very little about this important process during human development due to limited access to human embryos and ethical concerns作者: Inexorable 時(shí)間: 2025-3-31 19:11
Missile Defences and Asian-Pacific Security” ETiX-embryoids are formed from combined embryonic stem cells, trophoblast stem cells, and embryonic stem cells transiently induced to express Gata4. Cells are seeded into AggreWell dishes where they form aggregates that develop to resemble post-implantation mouse embryos following 4?days of cultur作者: Dna262 時(shí)間: 2025-4-1 01:21 作者: mucous-membrane 時(shí)間: 2025-4-1 05:17
Missile and Space Projects Guide 1962 bio-mechanical and bio-chemical cues, to regulate gene expression and influence cell fate. Deciphering such mechanisms is essential to understand early embryogenesis, as well as to harness differentiation disorders. Currently, several early developmental events remain unclear, mainly due to ethical作者: DOLT 時(shí)間: 2025-4-1 09:43 作者: exceptional 時(shí)間: 2025-4-1 11:22
https://doi.org/10.1007/978-1-4039-7854-7logical systems via comprehensive delineations of individual cellular states. Single-cell RNA sequencing in particular has served as a powerful tool to the study of the molecular circuitries underlying preimplantation embryonic development in both the mouse and human. Here we describe a method to el
