標題: Titlebook: Elispot for Rookies (and Experts Too); Sylvia Janetzki Book 2016 Springer International Publishing Switzerland 2016 B-cell Assay.Elispot.F [打印本頁] 作者: Jefferson 時間: 2025-3-21 19:11
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書目名稱Elispot for Rookies (and Experts Too)讀者反饋學科排名
作者: 雄偉 時間: 2025-3-21 21:29
Techniques in Life Science and Biomedicine for the Non-Experthttp://image.papertrans.cn/e/image/307703.jpg作者: 侵害 時間: 2025-3-22 01:28 作者: 歡樂東方 時間: 2025-3-22 07:36
Elispot for Rookies (and Experts Too)978-3-319-45295-1Series ISSN 2367-1114 Series E-ISSN 2367-1122 作者: REP 時間: 2025-3-22 09:18
Methoden der Marketingforschung (Aufgaben),Conducting an Elispot assay is a multistep process. The main steps are:作者: 高腳酒杯 時間: 2025-3-22 15:32
https://doi.org/10.1007/978-3-322-92052-2The choice of the Elispot plate can dramatically influence the final results of an Elispot experiment. Membrane-based 96-well Elispot plates are the optimal choice for Elispot. The membrane fibers look like a tightly woven web of polymers that offers a large surface for protein binding (Fig. 3.1).作者: 高腳酒杯 時間: 2025-3-22 20:46 作者: Laconic 時間: 2025-3-22 22:27 作者: Biguanides 時間: 2025-3-23 01:43
Gianfranco Walsh,David Dose,Maria SchwabeAs an important resource, a step-by-step Elispot protocol with important annotations is provided elsewhere [79].作者: 遣返回國 時間: 2025-3-23 09:18
https://doi.org/10.1007/978-3-658-11649-1Throughout the book we have touched upon various important controls, for the sample (apoptosis and viability, see Sect. 4.6), and for the assay (negative controls, sample-specific internal controls, nonspecific positive controls, external controls, see Sect. 5.1).作者: Pruritus 時間: 2025-3-23 11:13 作者: 旋轉(zhuǎn)一周 時間: 2025-3-23 15:16
Important Assay Details,Conducting an Elispot assay is a multistep process. The main steps are:作者: guardianship 時間: 2025-3-23 21:42
Reagents and Materials,The choice of the Elispot plate can dramatically influence the final results of an Elispot experiment. Membrane-based 96-well Elispot plates are the optimal choice for Elispot. The membrane fibers look like a tightly woven web of polymers that offers a large surface for protein binding (Fig. 3.1).作者: omnibus 時間: 2025-3-24 01:47 作者: 六個才偏離 時間: 2025-3-24 03:12
Planning the Experiment,A good Elispot experiment starts with a well-thought-through plan of execution. The quality of an experiment and its results are determined by the controls it includes. Here is a summary of important controls:作者: transient-pain 時間: 2025-3-24 09:59 作者: superfluous 時間: 2025-3-24 11:16
Quality Control,Throughout the book we have touched upon various important controls, for the sample (apoptosis and viability, see Sect. 4.6), and for the assay (negative controls, sample-specific internal controls, nonspecific positive controls, external controls, see Sect. 5.1).作者: Infiltrate 時間: 2025-3-24 14:57
Troubleshooting,This book has been structured in a way that it provides detailed information on choices about how to run an optimized Elispot assay. Throughout the book examples are given for what works and what does not and for good and suboptimal outcomes, and recommendations on how to prevent suboptimal outcomes and the reasoning behind it are provided.作者: Neolithic 時間: 2025-3-24 22:40 作者: Blemish 時間: 2025-3-25 02:25 作者: 多節(jié) 時間: 2025-3-25 04:06 作者: faddish 時間: 2025-3-25 08:52 作者: Antecedent 時間: 2025-3-25 15:12
Book 2016nderstand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as 作者: inculpate 時間: 2025-3-25 19:40
2367-1114 erstand the best options for specific protocol steps, reagen.This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provid作者: AORTA 時間: 2025-3-25 23:39 作者: 預(yù)示 時間: 2025-3-26 01:23
Customer Relationship Managementd so perfectly that you end up, for example, with exactly 60 antigen-specific cells in 200,000 cells added to the well, in three consecutive wells). While some variability is acceptable, the question is this: How much variability is acceptable? Let’s take a look at some hypothetical data sets:作者: 過分自信 時間: 2025-3-26 06:12
Data Analysis,d so perfectly that you end up, for example, with exactly 60 antigen-specific cells in 200,000 cells added to the well, in three consecutive wells). While some variability is acceptable, the question is this: How much variability is acceptable? Let’s take a look at some hypothetical data sets:作者: Medley 時間: 2025-3-26 09:01
Reporting Guidelines,erum used pretested? Were reported results background corrected? Or would you like to see an example image of crucial wells (which will likely be the best example available)? Did you wonder how the authors established reading parameters, and if reading parameters were adapted when necessary? Many more questions come to mind.作者: transplantation 時間: 2025-3-26 14:12
Overview,s in parts similar to Elispot, and from the fact that spots are the actual readout of the assay. Elispot has been around for many years. It was first described in 1983 for the detection of heat-labile enterotoxin production of bacteria [1], followed by its use for the enumeration of specific antibod作者: palpitate 時間: 2025-3-26 19:26 作者: Extricate 時間: 2025-3-26 21:15
Reporting Guidelines,? Once you know about the critical protocol steps of an Elispot assay, don’t you wonder how they were addressed by others? For example: How long it took to isolate PBMC after blood draw? How was resting performed, and for how long? What was the background reactivity for the tested samples? Was the s作者: Palpitation 時間: 2025-3-27 02:25
2367-1114 tion of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation..978-3-319-83260-9978-3-319-45295-1Series ISSN 2367-1114 Series E-ISSN 2367-1122 作者: insightful 時間: 2025-3-27 05:37 作者: 橫截,橫斷 時間: 2025-3-27 12:41
Book 2016. The most important feature of Elispot is its outstanding sensitivity, allowing the detection of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation..作者: 費解 時間: 2025-3-27 16:00 作者: arsenal 時間: 2025-3-27 21:47
The Evolution of Governance in the Australian National Basketball League, 1979–2013nal governing body) administrators who desired national club competition in a sport that was to boom as part of a global social trend. By the early 1990s, basketball was Australia’s fourth most popular spectator sport behind Australian football, rugby league and cricket. Yet the NBL was struggling s作者: 出來 時間: 2025-3-27 22:50
Book 2020rom a sphere when the electric current on its surface is turned on or off, either suddenly, gradually, or periodically..Numerical simulations are an invaluable source of information about this and related subjects, but they are often more difficult to interpret than exact, closed-form solutions that作者: Digest 時間: 2025-3-28 06:00
Book 2021ion. The contents will consider scientific, as well as innovative perspectives, on the problems related to the role of triatomine bugs as parasite vectors affecting millions in the Latin American region.作者: 啞巴 時間: 2025-3-28 09:41 作者: Ibd810 時間: 2025-3-28 14:13
Transformations in the Art of Dwelling: some Anthropological Reflections on Neolithic Houses,sion into island Southeast Asia, in search of illuminating parallels regarding aspects of dwelling such as kinship, rituals, the political uses of architecture and the role of the house in social memory.作者: 重畫只能放棄 時間: 2025-3-28 18:12 作者: ostracize 時間: 2025-3-28 22:24
maton recognizing .. needs to have at least .. states..Unfortunately, the alphabet for these languages grows with .. In order to prove a similar result for languages in a fixed alphabet we consider a counterpart of Hamming codes for permutations of finite sets, i.e. sets of permutations such that an作者: 大包裹 時間: 2025-3-29 02:10
Sirisha Velampalli,V. R. Murthy Jonnalageddara and security engineer and Black Hat speaker Darren Manners, as well as new commentaries by communications expert Andy Marken and DEF CON speaker Emily Peed. .The book offers practical advice for businesses, governments and individuals to better secure the world and protect cyberspace..978-3-319-37460-4978-3-319-17244-6作者: 小說 時間: 2025-3-29 06:27
Talent-Management in Schweizer KMU,led ‘geotourists’. Geotourists with a focus on ‘geological’ features form one end of a spectrum of geotourists with those having a more ‘geographical’ focus at the opposite end. Whilst starting with an interest in geology these geographical tourists are now much more interested in learning about the作者: Accolade 時間: 2025-3-29 10:35 作者: MAG 時間: 2025-3-29 11:46
