標(biāo)題: Titlebook: Electroporation and Sonoporation in Developmental Biology; Harukazu Nakamura Book 2009 Springer-Verlag Tokyo 2009 Expression.RNA.Tempo.Ver [打印本頁] 作者: Scuttle 時(shí)間: 2025-3-21 16:13
書目名稱Electroporation and Sonoporation in Developmental Biology影響因子(影響力)
書目名稱Electroporation and Sonoporation in Developmental Biology影響因子(影響力)學(xué)科排名
書目名稱Electroporation and Sonoporation in Developmental Biology網(wǎng)絡(luò)公開度
書目名稱Electroporation and Sonoporation in Developmental Biology網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Electroporation and Sonoporation in Developmental Biology被引頻次
書目名稱Electroporation and Sonoporation in Developmental Biology被引頻次學(xué)科排名
書目名稱Electroporation and Sonoporation in Developmental Biology年度引用
書目名稱Electroporation and Sonoporation in Developmental Biology年度引用學(xué)科排名
書目名稱Electroporation and Sonoporation in Developmental Biology讀者反饋
書目名稱Electroporation and Sonoporation in Developmental Biology讀者反饋學(xué)科排名
作者: 并排上下 時(shí)間: 2025-3-21 22:49 作者: abysmal 時(shí)間: 2025-3-22 00:45 作者: PLUMP 時(shí)間: 2025-3-22 06:28
Temporal Control of Gene Expression by Combining Electroporation and the Tetracycline Inducible Systinteract and regulate various aspects of vertebrate development in the last decade. This technique provides an efficient way to transfect embryonic cells in vivo with exogenous DNA by cre ating transient holes in the plasma membrane with short, squared electric pulses of low voltage (Itasaki et al.,作者: geometrician 時(shí)間: 2025-3-22 10:50 作者: sinoatrial-node 時(shí)間: 2025-3-22 14:20 作者: sinoatrial-node 時(shí)間: 2025-3-22 17:59
Enhancer Analysisatory regions in the genome, usually identified as enhancers (Kondoh, 2008). The identification and mapping of a set of enhancers allow clarification of essential regulatory elements involved in the enhancer action and their interacting protein factors. Enhancer analysis also determines upstream sig作者: Certainty 時(shí)間: 2025-3-22 21:16
Electroporation into the Limb: Beyond Misexpression with which to study pattern formation in vertebrates. We have used chick limb development to answer a simple but basic question, namely, why heterogeneous tissues are formed at correct positions and times from a homogeneous population of cells (Pearse & Tabin, 1998)..Limb development starts as two 作者: expire 時(shí)間: 2025-3-23 03:32 作者: 評論者 時(shí)間: 2025-3-23 07:05 作者: 啪心兒跳動(dòng) 時(shí)間: 2025-3-23 10:54 作者: 出來 時(shí)間: 2025-3-23 15:17 作者: 背叛者 時(shí)間: 2025-3-23 19:28
In Utero Electroporation: Assay System for Migration of Cerebral Cortical Neuronse and then migrate toward the pial surface to form the highly organized 6-layered cerebral cortex. Detailed profiles of these processes and their molecular mechanisms had been largely unknown because of the absence of an efficient assay system. The in vivo electroporation system was initially devise作者: Spinal-Fusion 時(shí)間: 2025-3-24 00:47 作者: Ligament 時(shí)間: 2025-3-24 04:55
Single Cell Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Culturesrevealing many neural connection patterns in the CNS (Itaya and van Hoesen, 1982; Fabian and Coulter, 1985; Yoshihara, 2002). Moreover, a tracing method with fluorescent dye has enabled the observation of growing axons in living conditions, and demon strated a lot of developmental aspects in axon gr作者: Flavouring 時(shí)間: 2025-3-24 09:44 作者: 軟弱 時(shí)間: 2025-3-24 13:40
Gene Transfer into Mouse Haemogenic Sites, as a Mean to Functionally Approach the Control of Mesoderf two independently generated pools of haematopoietic precursors. These two haematopoietic precursors waves are dedicated to specialized functions regarding haematopoiesis ontogeny. The first, which occurs in the extra-embryonic compartment, in the yolk Sac (YS) blood islands, rapidly produces the d作者: 不透明 時(shí)間: 2025-3-24 17:13 作者: jettison 時(shí)間: 2025-3-24 21:25 作者: 構(gòu)想 時(shí)間: 2025-3-25 00:06
The External Accounting Framework,of in ovo electroporation. Here, we show the application of in ovo electroporation to the developmental neural tube in chick embryo. electroporation, only the anode side is transfected and the cathode side could be used as the control (Funahashi et al., 1999; Nakamura et al., 2000; Odani et al., 2008).作者: 赦免 時(shí)間: 2025-3-25 06:35
The Future of Earth’s Climate After Parisiwa et al., 1991; Sato et al., 2007). We applied this system for tracing retinal fibers (Harada et al., 2008). In this chapter, we demonstrate the method of tracing retinal fibers from both small and large groups of the retinal ganglion cell (RGC) with transposon-mediated gene transfer by in ovo electroporation to chick embryos.作者: 一小塊 時(shí)間: 2025-3-25 10:30 作者: 屈尊 時(shí)間: 2025-3-25 15:21 作者: 革新 時(shí)間: 2025-3-25 16:54
Book 2009 and loss of function in desired tissue at any desired stage in chick embryos. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of 作者: 嘴唇可修剪 時(shí)間: 2025-3-25 23:10
Book 2009the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced. .作者: Myofibrils 時(shí)間: 2025-3-26 02:26 作者: 嫌惡 時(shí)間: 2025-3-26 07:31
Electroporation and Sonoporation in Developmental Biology作者: Etymology 時(shí)間: 2025-3-26 09:33
Electroporation and Sonoporation in Developmental Biology978-4-431-09427-2作者: indemnify 時(shí)間: 2025-3-26 13:42 作者: 兇兆 時(shí)間: 2025-3-26 18:07 作者: 善于騙人 時(shí)間: 2025-3-26 22:29
Temporal Control of Gene Expression by Combining Electroporation and the Tetracycline Inducible Systplication of a small current through the targeted area. To date, all three germ layers — endoderm, mesoderm and ectoderm — as well as an increasing number of differentiated structures have been efficiently transfected (Dubrulle et al., 2001; Grapin-Botton et al., 2001; Itasaki et al., 1999; Luo and 作者: 制造 時(shí)間: 2025-3-27 03:31
In Ovo Electroporation for Targeting the Somitic Mesodermion-mediated transgenesis. However, as long as the PSM was targeted, transgenesis was not successful for unknown reasons. Several years ago, we achieved somitic transgenesis by targeting PSM precursors (presumptive somitic cells) of earlier embryos, the cells residing in the epiblast of the anterior作者: alcoholism 時(shí)間: 2025-3-27 08:30
Method of Electroporation for the Early Chick Embryooses, a whole embryo culture system, originally invented by Dennis A. T. New (New, 1955), and its derivatives (Flamme, 1987; Sundin and Eichele, 1992; Stern, 1993; Chapman et al., 2001) have been widely used..Here we describe a method of electroporation for the early chick embryos using the . whole-作者: BILK 時(shí)間: 2025-3-27 10:43 作者: Mhc-Molecule 時(shí)間: 2025-3-27 16:09
Electroporation into the Limb: Beyond Misexpressionn formation during limb bud stages. Following establishment of these axes, one can visually recognize condensation of cartilages. Muscles, tendons, and neurons migrate and differentiate after cartilage formation. Because the stages and events are easily recognized morphologically and in detail, it i作者: Compass 時(shí)間: 2025-3-27 19:38
Retroviral Vector-Mediated Gene Transfer into the Chick Optic Vesicle by In Ovo Electroporationonal analysis of genes has become possible in combination with classical techniques of developmental biology and neurobiology..One general method is replication-competent retroviral particle-mediated gene transfer in the chick embryo (Morgan and Fekete, 1996). After the infection of viral particles,作者: orient 時(shí)間: 2025-3-28 00:36
Clonal and Widespread Gene Transfer by Proviral Electroporation for Analysis of Brain Laminar Format1; Sugiyama and Nakamura, 2003; Huber et al., 2008)..In spatial terms, widespread gene transfer by electroporation has provided an efficient way to unveil a new gene function on a given tissue (Nakamura and Funahashi, 2001). On the other hand, the spatial precision of gene transfer also has been add作者: 媒介 時(shí)間: 2025-3-28 05:34 作者: Morose 時(shí)間: 2025-3-28 08:23
In Utero Electroporation: Assay System for Migration of Cerebral Cortical Neuronsal., 2002). In utero electroporation of other sites in the brain, including the hippocampus (Navarro-Quiroga et al., 2007), cerebral basal ganglia (Borrell et al., 2005; Nakahira et al., 2006), cortical hem (Takiguchi-Hayashi et al., 2004), and dorsal thalamus (Bonnin et al., 2007), has recently bee作者: NAV 時(shí)間: 2025-3-28 13:34 作者: 認(rèn)識 時(shí)間: 2025-3-28 15:18
Single Cell Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Culturesruber et al., 2001; O‘Brien et al., 2001; Ma et al., 2002; Sahly et al., 2003). In particular, the electroporation technique was proved as a valuable tool, since it can be applied to a wide range of tissues and cell types with little toxicity and can be performed with relative technical easiness. Mo作者: deciduous 時(shí)間: 2025-3-28 19:27 作者: 加花粗鄙人 時(shí)間: 2025-3-29 01:55
Gene Transfer into Mouse Haemogenic Sites, as a Mean to Functionally Approach the Control of Mesoderaintenance of haematopoiesis (Cumano and Godin, 2007)..In lower vertebrate species, such as Avian and Amphibian, the independent generation of extra- and intra-embryonic precursors pools, as well as the transient nature of the extra-embryonic one, was demonstrated through the use of interspecific ch作者: 機(jī)械 時(shí)間: 2025-3-29 05:41
Electroporation of Embryonic Kidney Explantsring RNA (Davies et al., 2004), by treating with pharmaceutical agents (Tang et al., 2002), or by using viral transduction to overexpress mutant proteins (Favre et al., 2000). More recently, microinjection followed by electroporation has been used to successfully express plasmid DNA encoding protein作者: obviate 時(shí)間: 2025-3-29 10:40 作者: GRATE 時(shí)間: 2025-3-29 13:55 作者: Debility 時(shí)間: 2025-3-29 16:25
International Cases of Corporate Governancehickens has provided numerous novel insights into the understanding of early development in vertebrates, making the chicken a unique model animal..One of few shortfalls of the original technique has been that expression of electroporated genes does not persist for a long period of time probably beca作者: 思考而得 時(shí)間: 2025-3-29 21:26 作者: tariff 時(shí)間: 2025-3-30 00:47
International Cell Biology 1980–1981ion-mediated transgenesis. However, as long as the PSM was targeted, transgenesis was not successful for unknown reasons. Several years ago, we achieved somitic transgenesis by targeting PSM precursors (presumptive somitic cells) of earlier embryos, the cells residing in the epiblast of the anterior作者: Arctic 時(shí)間: 2025-3-30 05:51
International Citizens‘ Tribunalsoses, a whole embryo culture system, originally invented by Dennis A. T. New (New, 1955), and its derivatives (Flamme, 1987; Sundin and Eichele, 1992; Stern, 1993; Chapman et al., 2001) have been widely used..Here we describe a method of electroporation for the early chick embryos using the . whole-作者: BUCK 時(shí)間: 2025-3-30 10:59
Parenchymal Changes of CT Imaginggation of regulatory regions associated with a gene, various animal systems and tissue-cultured cells are widely employed, each having unique advan tages and drawbacks. We find that electroporation of chicken embryos is most advantageous for systematic survey of development-associated regulatory reg作者: 巨頭 時(shí)間: 2025-3-30 14:48 作者: 黃油沒有 時(shí)間: 2025-3-30 19:40 作者: AIL 時(shí)間: 2025-3-31 00:06
Muhamad Fairus Kamaruzaman,Riaza Mohd Rias1; Sugiyama and Nakamura, 2003; Huber et al., 2008)..In spatial terms, widespread gene transfer by electroporation has provided an efficient way to unveil a new gene function on a given tissue (Nakamura and Funahashi, 2001). On the other hand, the spatial precision of gene transfer also has been add作者: Enthralling 時(shí)間: 2025-3-31 02:04
https://doi.org/10.1007/978-981-10-4460-1to manipulate gene expression, these techniques take much time and financial costs, which limit their use..Whole-embryo mammalian culture system was established by New and colleague in the 1970s, and was modified thereafter by several researchers (reviewed by New, 1971, 1978; Sturm and Tam, 1993; Ho作者: TEM 時(shí)間: 2025-3-31 08:41
al., 2002). In utero electroporation of other sites in the brain, including the hippocampus (Navarro-Quiroga et al., 2007), cerebral basal ganglia (Borrell et al., 2005; Nakahira et al., 2006), cortical hem (Takiguchi-Hayashi et al., 2004), and dorsal thalamus (Bonnin et al., 2007), has recently bee作者: Oscillate 時(shí)間: 2025-3-31 10:08 作者: PLUMP 時(shí)間: 2025-3-31 16:48 作者: Generic-Drug 時(shí)間: 2025-3-31 18:09 作者: Enthralling 時(shí)間: 2025-4-1 01:16
https://doi.org/10.1007/978-1-4757-5563-3aintenance of haematopoiesis (Cumano and Godin, 2007)..In lower vertebrate species, such as Avian and Amphibian, the independent generation of extra- and intra-embryonic precursors pools, as well as the transient nature of the extra-embryonic one, was demonstrated through the use of interspecific ch作者: Instinctive 時(shí)間: 2025-4-1 03:51
C. J. P. M. van Mosseveld,P. van Sonring RNA (Davies et al., 2004), by treating with pharmaceutical agents (Tang et al., 2002), or by using viral transduction to overexpress mutant proteins (Favre et al., 2000). More recently, microinjection followed by electroporation has been used to successfully express plasmid DNA encoding protein作者: 事物的方面 時(shí)間: 2025-4-1 07:30
The Future of the Lucas Paradox,ited to bac teria trans formation. Nevertheless, electroporation in higher organisms has been carried out for the transfer of the drugs for treatment of cancer; bleomycin was successfully applied to hepatocellular carcinoma in the Donryu rat (Okino and Mohri, 1987). Mr. Hayakawa (Nepa Gene; Ichikawa作者: FORGO 時(shí)間: 2025-4-1 12:55
The External Accounting Framework,plication. In chick embryos, the in ovo electroporation technique has enabled molecular analysis of developmental mechanisms in chick embryos and has revived chick embryos as the model animals in developmental biology (Muramatsu et al., 1997; Sakamoto et al., 1998; Funahashi et al., 1999; Nakamura e
