標(biāo)題: Titlebook: Electrophoretic Separation of Proteins; Methods and Protocol Biji T. Kurien,R. Hal Scofield Book 2019 Springer Science+Business Media, LLC, [打印本頁] 作者: 老鼠系領(lǐng)帶 時間: 2025-3-21 18:19
書目名稱Electrophoretic Separation of Proteins影響因子(影響力)
書目名稱Electrophoretic Separation of Proteins影響因子(影響力)學(xué)科排名
書目名稱Electrophoretic Separation of Proteins網(wǎng)絡(luò)公開度
書目名稱Electrophoretic Separation of Proteins網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Electrophoretic Separation of Proteins被引頻次
書目名稱Electrophoretic Separation of Proteins被引頻次學(xué)科排名
書目名稱Electrophoretic Separation of Proteins年度引用
書目名稱Electrophoretic Separation of Proteins年度引用學(xué)科排名
書目名稱Electrophoretic Separation of Proteins讀者反饋
書目名稱Electrophoretic Separation of Proteins讀者反饋學(xué)科排名
作者: Oversee 時間: 2025-3-22 00:11
Measuring Protein Concentration with Absorbance, Lowry, Bradford Coomassie Blue, or the Smith Bicinn procedures. Decisions on the usefulness of any protein isolation procedure depend on knowing the concentration of proteins before and after a procedure. Protein concentration in solution is generally measured with spectrophotometry in the UV range or in the presence of dyes or copper interacting w作者: 神經(jīng) 時間: 2025-3-22 03:23
Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three-Phase Partitd undesirable molecules, to separate protein fractions, or to increase protein concentrations. Proteins can be concentrated by precipitation from solution with ammonium sulfate, polyethylene glycol, organic solvents, trichloroacetic acid, potassium chloride/sodium dodecyl sulfate thermal denaturatio作者: 大罵 時間: 2025-3-22 08:03
Lysis Buffer Choices Are Key Considerations to Ensure Effective Sample Solubilization for Protein Eocal adhesion protein Kindlin-2 and the transcriptional repressor Snail from choriocarcinoma cells using NP-40 and RIPA lysis buffer. We also show the use of a more denaturing urea/thiourea lysis buffer for solubilization, by comparing its effectiveness with the often utilized RIPA lysis buffer for 作者: ostracize 時間: 2025-3-22 08:51
The Cydex Blue Assay: A One-Step Protein Assay for Samples Prior to SDS Electrophoresis,d reducers in the buffers used for protein extraction. Reducers interfere with the copper-based assays, while SDS interferes with the dye-binding assays. The combined use of cyclodextrins with a commercial Bradford reagent concentrate, described in this chapter, allows to determine protein concentra作者: 詩集 時間: 2025-3-22 13:37
Cellulose Acetate Electrophoresis of Hemoglobin,t other than electrophoresis apparatus and the cellulose acetate strip. Here we describe a modified version of cellulose acetate membrane electrophoresis for hemoglobin separation from blood sample. Sharp, clear bands without tailing effects can be obtained with this method. The method and apparatus作者: 詩集 時間: 2025-3-22 20:06
Native Polyacrylamide Gels,) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the mixture to be analyzed and to provide all proteins with a negative charge. As a consequence, the proteins will be separ作者: alliance 時間: 2025-3-22 21:58
Isoelectric Focusing on Non-Denaturing Flatbed Gels,ody clonotype changes. Here we discuss the use of a sensitive native flatbed isoelectric focusing method to analyze specific antibody clonotype changes in a patient with systemic lupus erythematosus, who developed autoantibodies to the Ro 60 autoantigen under observation. Patient sera samples collec作者: HILAR 時間: 2025-3-23 02:00
Determination of Protein Molecular Weights on SDS-PAGE,odium dodecyl sulfate (SDS) separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This method was established around 1969 and has been utilized substantially even today because of its simplicity. During the following half a century, although it has been reported that man作者: 金哥占卜者 時間: 2025-3-23 08:13 作者: 強行引入 時間: 2025-3-23 13:01
Cationic Electrophoresis, where this method produces suboptimal results. In these cases electrophoresis in the presence of positively charged detergents like cetyltrimethylammonium bromide (CTAB) may work considerably better. Methods for electrophoresis and staining of such gels are presented.作者: Friction 時間: 2025-3-23 16:43 作者: indubitable 時間: 2025-3-23 18:56 作者: 輕彈 時間: 2025-3-23 23:33
Tricine-SDS-PAGE,wever, the standard system is quite complicated and specifically may not be useful when the separated proteins are to be recovered from the gel for quantitative analysis. Here, we describe a simplified system whereby these smaller proteins can be resolved in comparatively low percentage gels which h作者: Distribution 時間: 2025-3-24 05:54
Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis,orm of glycation, which have been implicated in diseases such as diabetes, Alzheimer’s disease, autoimmune diseases, and cancer. The analysis of glycated proteins is challenging due to their complexity and variability. Numerous analytical techniques have been developed that require expensive special作者: 一大塊 時間: 2025-3-24 07:40
Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies, zone electrophoresis or isoelectric focusing. Most commonly antigens (which are often immunoglobulins) are separated by electrophoresis followed by precipitation with specific antibodies in situ. However, immunoglobulins with specific reactivity can be also precipitated with the proper antigens aft作者: HALL 時間: 2025-3-24 14:32 作者: 暗語 時間: 2025-3-24 16:49 作者: cushion 時間: 2025-3-24 20:58
Book 2019g and avoiding known pitfalls..Practical and thorough, .Electrophoretic Separation of Proteins: Methods and Protocols. is a valuable resource for researchers who are interested in learning and experimenting with this field..作者: Asparagus 時間: 2025-3-25 03:02
https://doi.org/10.1007/978-3-8349-9230-7iscerned in these various gels played an indispensable role in determining the nature of the change in the haptoglobin gene (.) that leads to the polymeric series characteristic of ../.. homozygotes. Where possible the names of scientific friends who made this saga of gel electrophoresis so memorabl作者: 施加 時間: 2025-3-25 05:17
Transport Economy, Policy and Location,ibody (.) to the protein chain. Contrary to SDS, sarcosyl only binds to the amino acid chain of the PEGylated protein and thus leads to a sharper electrophoretic band and enhanced antibody binding. While the method was originally developed for anti-doping purposes, it may also be useful for the elec作者: FECT 時間: 2025-3-25 07:45
How It All Began: A Personal History of Gel Electrophoresis,iscerned in these various gels played an indispensable role in determining the nature of the change in the haptoglobin gene (.) that leads to the polymeric series characteristic of ../.. homozygotes. Where possible the names of scientific friends who made this saga of gel electrophoresis so memorabl作者: 類似思想 時間: 2025-3-25 12:33 作者: 維持 時間: 2025-3-25 16:21 作者: 催眠藥 時間: 2025-3-25 21:44
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: Desert 時間: 2025-3-26 03:50
Electrophoretic Separation of Proteins978-1-4939-8793-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: chance 時間: 2025-3-26 07:56
Biji T. Kurien,R. Hal ScofieldIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Boycott 時間: 2025-3-26 09:09
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/306513.jpg作者: acetylcholine 時間: 2025-3-26 15:33
Two-Sided Markets and Interchange Fees, where this method produces suboptimal results. In these cases electrophoresis in the presence of positively charged detergents like cetyltrimethylammonium bromide (CTAB) may work considerably better. Methods for electrophoresis and staining of such gels are presented.作者: 故意 時間: 2025-3-26 19:28
Cationic Electrophoresis, where this method produces suboptimal results. In these cases electrophoresis in the presence of positively charged detergents like cetyltrimethylammonium bromide (CTAB) may work considerably better. Methods for electrophoresis and staining of such gels are presented.作者: prosthesis 時間: 2025-3-26 22:14 作者: Fallibility 時間: 2025-3-27 03:49
https://doi.org/10.1007/978-3-8349-9230-7is with a variety of supporting media (e.g., filter paper or starch grains) was beginning to replace it. This chapter is an account of 10?years of experiments carried out by the author during which molecular sieving gel electrophoresis was developed and common genetic variants of two proteins, hapto作者: 爵士樂 時間: 2025-3-27 07:20
DSS-Komponente Prognosesteuerung,n procedures. Decisions on the usefulness of any protein isolation procedure depend on knowing the concentration of proteins before and after a procedure. Protein concentration in solution is generally measured with spectrophotometry in the UV range or in the presence of dyes or copper interacting w作者: DNR215 時間: 2025-3-27 11:52 作者: 貪婪的人 時間: 2025-3-27 14:14 作者: 寬容 時間: 2025-3-27 18:26 作者: Additive 時間: 2025-3-27 23:18 作者: FRONT 時間: 2025-3-28 05:20
Intercellular Communication and Cancer,) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the mixture to be analyzed and to provide all proteins with a negative charge. As a consequence, the proteins will be separ作者: conscribe 時間: 2025-3-28 10:15
Harini Krishnan,Gary S. Goldbergody clonotype changes. Here we discuss the use of a sensitive native flatbed isoelectric focusing method to analyze specific antibody clonotype changes in a patient with systemic lupus erythematosus, who developed autoantibodies to the Ro 60 autoantigen under observation. Patient sera samples collec作者: 館長 時間: 2025-3-28 14:11 作者: dilute 時間: 2025-3-28 18:11
Yael Friedmann,Charles W. Danielfrom a complex mixture. Two-dimensional gel electrophoresis established more than four decades ago serves?as a powerful protocol to isolate many proteins at once for such protein analysis. In the first two decades, the original procedure to use a glass tube-based IEF had been commonly used. Since an作者: cravat 時間: 2025-3-28 20:48 作者: 欲望 時間: 2025-3-29 01:35
Natural Vegetation in Agroecosystems,n combined with sodium dodecyl sulfate polyacrylamide gel electrophoresis, this procedure allows for high-resolution separation of cellular proteins for analytical purposes. Laboratories perform IEF by (a) using carrier ampholytes that migrate through a gel to create the pH gradient or (b) using imm作者: Neutral-Spine 時間: 2025-3-29 03:08 作者: carbohydrate 時間: 2025-3-29 08:54 作者: 去世 時間: 2025-3-29 12:52
J. Davis,R. Venkatesan,J. Meindlorm of glycation, which have been implicated in diseases such as diabetes, Alzheimer’s disease, autoimmune diseases, and cancer. The analysis of glycated proteins is challenging due to their complexity and variability. Numerous analytical techniques have been developed that require expensive special作者: Critical 時間: 2025-3-29 16:25 作者: 靦腆 時間: 2025-3-29 20:21
Crosstalk Effects in Digital Circuits,has been developed that has vastly improved resolving power for very large proteins. Proteins with molecular masses between 200 and 4000?kDa can be clearly separated. Inclusion of a reducing agent in the upper reservoir buffer and use of a large pore-sized agarose have been found to be key technical作者: 空氣傳播 時間: 2025-3-30 01:58
J. Dambre,H. Van Marck,J. Van Campenhoutch as diffusion and nonspecific trapping in the gel matrix. These negative factors reduce protein concentrations within a defined gel volume with increasing migration distance and, therefore, decrease protein recovery efficiency. Here, we describe the enhancement of protein separation efficiency for作者: 讓步 時間: 2025-3-30 05:08 作者: JECT 時間: 2025-3-30 09:08
Introduction to Protein Electrophoresis,This chapter briefly discusses the developments in electrophoresis of proteins from Tiselius’ moving-boundary electrophoresis to the modern-day two-dimensional polyacrylamide gel electrophoresis. It also touches upon the staining methods used to visualize total proteins post electrophoresis.作者: 搖曳 時間: 2025-3-30 16:20 作者: AVID 時間: 2025-3-30 18:16 作者: fiction 時間: 2025-3-30 21:47 作者: Entreaty 時間: 2025-3-31 01:47
Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis,etect, identify, and analyze these posttranslational modifications. This new cost-effective methodology will aid the detection of unwanted glycation products in processed foods and may lead to new diagnostics and therapeutics for age-related chronic diseases.作者: ESPY 時間: 2025-3-31 07:26 作者: Cytology 時間: 2025-3-31 10:23 作者: 痛打 時間: 2025-3-31 16:44
Lysis Buffer Choices Are Key Considerations to Ensure Effective Sample Solubilization for Protein Esolubilization of heat shock proteins (HSP) B1 and B5 and the cytoplasmic adapter protein integrin-linked kinase (ILK) from smooth muscle. Overall, the results demonstrate the importance of optimizing lysis buffers for specific protein solubilization prior to finalizing the experimental workflow.作者: 偽書 時間: 2025-3-31 18:38 作者: Rotator-Cuff 時間: 2025-4-1 00:47
Isoelectric Focusing on Non-Denaturing Flatbed Gels,ted over several years were used for analysis using flatbed IEF. Following electrofocusing, the gel is analyzed by affinity immunoblotting utilizing Ro 60-coated nitrocellulose membrane to determine oligoclonality of the anti-Ro 60 containing sera.作者: 倔強一點 時間: 2025-4-1 03:30 作者: Offensive 時間: 2025-4-1 08:18
1064-3745 ation advice from the expertsThis volume expands upon?.Protein Electrophoresis. (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as the Cydex Blue assay; cellulose-acetate electrophoresis of hemoglobin; cat作者: reserve 時間: 2025-4-1 13:04
Screening Functions in Simple Metalssis for hemoglobin separation from blood sample. Sharp, clear bands without tailing effects can be obtained with this method. The method and apparatus described here would be appropriate to separate protein fractions under 1?h at voltages up to 60?V/cm measured between the electrodes.作者: 細胞學(xué) 時間: 2025-4-1 15:22
Mohamed A. Elgamel,Magdy A. Bayoumiantitative analysis. Here, we describe a simplified system whereby these smaller proteins can be resolved in comparatively low percentage gels which have high compatibility with modern detectors such as UV and inductively coupled plasma-mass spectrometry (ICP-MS).
