標題: Titlebook: Electron Microscopy; Methods and Protocol John Kuo Book 2014Latest edition Springer Science+Business Media, New York 2014 (EM) data.Cryo-sp [打印本頁] 作者: 租期 時間: 2025-3-21 16:06
書目名稱Electron Microscopy影響因子(影響力)
書目名稱Electron Microscopy影響因子(影響力)學科排名
書目名稱Electron Microscopy網絡公開度
書目名稱Electron Microscopy網絡公開度學科排名
書目名稱Electron Microscopy被引頻次
書目名稱Electron Microscopy被引頻次學科排名
書目名稱Electron Microscopy年度引用
書目名稱Electron Microscopy年度引用學科排名
書目名稱Electron Microscopy讀者反饋
書目名稱Electron Microscopy讀者反饋學科排名
作者: 誤傳 時間: 2025-3-21 23:58 作者: 消耗 時間: 2025-3-22 02:37
A ‘Soldier of the Third International’paration of APS-mica surfaces and the preparation of samples for AFM imaging. The protocol for synthesis and purification of APS is also provided. The AFM applications are illustrated with examples of images of DNA and protein–DNA complexes.作者: CHART 時間: 2025-3-22 07:41
History of Social Law in Germanyhods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.作者: 預防注射 時間: 2025-3-22 10:07 作者: galley 時間: 2025-3-22 16:53
Tracking DNA and RNA Sequences at High Resolution,hods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.作者: galley 時間: 2025-3-22 17:23
Biological Applications of Phase-Contrast Electron Microscopy,Zernike PC cryo-electron microscopy to biological systems such as protein molecules, virus particles, and cells, including single-particle analysis to delineate three-dimensional (3D) structures of protein and virus particles and cryo-electron tomography to reconstruct 3D images of complex protein systems and cells.作者: ASTER 時間: 2025-3-23 00:43
1064-3745 .Contains key notes and implementation advice from the exper.This third edition of .Electron Microscopy: Methods and Protocols .expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related im作者: 陳舊 時間: 2025-3-23 02:52
https://doi.org/10.1007/978-3-031-36892-9mples in aluminum specimen carriers, cell cultures grown on Sapphire discs, suspensions for freeze-fracturing, and specimens for cryo-sectioning. Additionally, we include an advanced technique to freeze monolayer cell cultures on Sapphire discs with the Leica EM PACT2 HPF machine using a composite carrier.作者: prolate 時間: 2025-3-23 06:07 作者: preservative 時間: 2025-3-23 09:47 作者: 散步 時間: 2025-3-23 16:33
,Visualization of DNA and Protein–DNA Complexes with Atomic Force Microscopy,paration of APS-mica surfaces and the preparation of samples for AFM imaging. The protocol for synthesis and purification of APS is also provided. The AFM applications are illustrated with examples of images of DNA and protein–DNA complexes.作者: Infusion 時間: 2025-3-23 20:29 作者: 背書 時間: 2025-3-23 23:50 作者: BAIL 時間: 2025-3-24 03:24
Philosophy of Religion in Australasia, are described for handling cells grown in liquid culture as well as on substrates such as culture dishes, slide culture chambers, or agar. These protocols may be used to process not only cultured organisms but also larger botanical and zoological specimens.作者: hyperuricemia 時間: 2025-3-24 07:17 作者: 完整 時間: 2025-3-24 13:06 作者: GUILT 時間: 2025-3-24 16:40 作者: biosphere 時間: 2025-3-24 22:24
Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specime are described for handling cells grown in liquid culture as well as on substrates such as culture dishes, slide culture chambers, or agar. These protocols may be used to process not only cultured organisms but also larger botanical and zoological specimens.作者: Iniquitous 時間: 2025-3-25 03:05 作者: 感激小女 時間: 2025-3-25 04:32 作者: ETCH 時間: 2025-3-25 10:30 作者: constellation 時間: 2025-3-25 15:25
https://doi.org/10.1007/978-1-62703-776-1(EM) data; Cryo-specimen; DNA and RNA tracking; Focussed Ion Beam (FIB); Scanning Electron Microscope (S作者: EXTOL 時間: 2025-3-25 17:20 作者: 安心地散步 時間: 2025-3-25 20:16
Electron Microscopy978-1-62703-776-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: municipality 時間: 2025-3-26 01:57 作者: 減至最低 時間: 2025-3-26 08:14 作者: Osteons 時間: 2025-3-26 11:44 作者: Morbid 時間: 2025-3-26 16:36
Gary W. Barrett,Terry L. Barrett,Jianguo Wuructures. Often specimens are fixed and stained with osmium tetroxide during fixation, but additional contrast is the result of additional heavy metal stains on the sections. The most common post-staining of sections is done on grids by aqueous uranyl acetate followed by lead citrate. When it is app作者: Integrate 時間: 2025-3-26 17:18
The Construction Problems of Antiquity,ture of biological specimens. The actual procedure for producing a metal shadow is relatively simple; a heavy metal is evaporated from a source at an oblique angle to the specimen. The metal atoms pile up on the surfaces that face the source, but the surfaces away from the source are shielded and re作者: 業(yè)余愛好者 時間: 2025-3-26 23:55
Auxiliary Tools in Meteorology,aling broad panoramas of membrane interior. These large panoramas reveal the three-dimensional contours of membranes making the methods well suited to studying changes in membrane architecture. Freshly split membrane faces are visualized by platinum or tungsten shadowing and carbon backing to form a作者: 含沙射影 時間: 2025-3-27 02:08
Philosophy of Religion in Australasia, are described for handling cells grown in liquid culture as well as on substrates such as culture dishes, slide culture chambers, or agar. These protocols may be used to process not only cultured organisms but also larger botanical and zoological specimens.作者: Gastric 時間: 2025-3-27 07:17 作者: 范圍廣 時間: 2025-3-27 10:54
The Rule of Law and Rule-Bound Ordersanisms. Embedded in vitreous ice, such samples can be further processed by freeze substitution or directly analyzed in their fully hydrated state by cryo-electron microscopy of vitreous sections (CEMOVIS) to explore cellular ultrastructure as close as possible to the native state. Here, we describe 作者: 折磨 時間: 2025-3-27 15:36
History of Psychiatry and Medical Psychologyotectant [1, 2]. This opened the opportunity to cryo-electron microscopy (cryo-EM) to observe biological samples at nanometer scale, close to their native state. However, poor electron penetration through biological samples sets the limit for sample thickness to less than the average size of the mam作者: PANIC 時間: 2025-3-27 21:18 作者: 擴大 時間: 2025-3-27 23:52
The Setting and Initial Events,pindles and are readily fractionated for biochemical assays, providing a good tool to dissect molecular mechanism. We developed techniques for immunoelectron microscopy of microtubule structures assembled in perfusion chambers and in solution.作者: Vertical 時間: 2025-3-28 03:34 作者: 節(jié)約 時間: 2025-3-28 06:16 作者: Gobble 時間: 2025-3-28 13:21 作者: Cacophonous 時間: 2025-3-28 16:57
History of Social Law in Germanyed primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most f作者: NADIR 時間: 2025-3-28 20:09
A ‘Soldier of the Third International’tion of the mica surface with aminopropyl silatrane (APS) to yield an APS-mica surface. This surface binds nucleic acids and nucleoprotein complexes in a wide range of ionic strengths, in the absence of divalent cations, and in a broad range of pH. The chapter describes the methodologies for the pre作者: Spina-Bifida 時間: 2025-3-28 23:50 作者: Odyssey 時間: 2025-3-29 03:04 作者: 反感 時間: 2025-3-29 07:37 作者: 莊嚴 時間: 2025-3-29 13:08
Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy,nsmission electron microscopy. This chapter describes a microwave-assisted protocol for processing, dehydrating and embedding biological material, taking them from living specimens to blocks embedded in sectionable resin in 4 h or less.作者: 輕信 時間: 2025-3-29 16:07
Processing Plant Tissues for Ultrastructural Study,ying the cellular and organelle ultrastructure of plant tissues under transmission electron microscopy. The general methods and procedures for the plant specimen preparation (including fixation, dehydration, resin infiltration, and embedding) are similar to those for animal tissues. However, certain作者: mettlesome 時間: 2025-3-29 23:36
Staining Sectioned Biological Specimens for Transmission Electron Microscopy: Conventional and , Structures. Often specimens are fixed and stained with osmium tetroxide during fixation, but additional contrast is the result of additional heavy metal stains on the sections. The most common post-staining of sections is done on grids by aqueous uranyl acetate followed by lead citrate. When it is app作者: 辮子帶來幫助 時間: 2025-3-30 03:32 作者: 巨碩 時間: 2025-3-30 06:24 作者: AVOID 時間: 2025-3-30 11:44
Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specime are described for handling cells grown in liquid culture as well as on substrates such as culture dishes, slide culture chambers, or agar. These protocols may be used to process not only cultured organisms but also larger botanical and zoological specimens.作者: 爭吵加 時間: 2025-3-30 16:23
High-Pressure Freezing: Current State and Future Prospects,l due to significant changes compared to its predecessor model. Its centerpiece is a multipart polymer cartridge which holds the specimen carrier sandwich and guides it automatically through the freezing process until immersed in liquid nitrogen. The cartridge can be adapted to the specimen and carr作者: 拘留 時間: 2025-3-30 16:38 作者: fixed-joint 時間: 2025-3-31 00:07 作者: ASSET 時間: 2025-3-31 01:17 作者: 高度贊揚 時間: 2025-3-31 05:48 作者: CRACK 時間: 2025-3-31 09:16 作者: 冷漠 時間: 2025-3-31 16:16 作者: 可忽略 時間: 2025-3-31 17:58
Cryo-electron Microscopy of Membrane Proteins,tubular crystals. This method has been used to determine the atomic resolution structures of bacteriorhodopsin, tubulin, aquaporins, and several other membrane proteins. In addition, a large number of membrane protein structures were studied at a slightly lower resolution, whereby at least secondary作者: Initiative 時間: 2025-4-1 01:19
Tracking DNA and RNA Sequences at High Resolution,ed primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most f作者: 開始發(fā)作 時間: 2025-4-1 03:23 作者: CROAK 時間: 2025-4-1 08:59 作者: 鋸齒狀 時間: 2025-4-1 14:05
Single Particle Cryo-electron Microscopy and 3-D Reconstruction of Viruses,3-D reconstruction of icosahedral viruses has now reached near-atomic resolutions (3–4 ?). With comparable resolutions and more predictable outcomes, cryo-EM is now considered a preferred method over X-ray crystallography for determination of atomic structure of icosahedral viruses. At near-atomic r作者: badinage 時間: 2025-4-1 16:46
Ancient Eastern-Iranian Culturerom the tissues and resin. Microwave-assisted procedure can reduce specimen preparation time, but both conventional and microwave-assisted chemical fixation procedures produce artifacts. Cryo-specimen preparation involves with high-pressure freezing and freeze-substitution can minimize artifact form作者: 無表情 時間: 2025-4-1 21:21
Gary W. Barrett,Terry L. Barrett,Jianguo Wurall improved visualization of ultrastructural details in biological specimens. Tannic acid and PPD improve membrane preservation, and malachite green is a phospholipid stain. All of these stains are compatible with aqueous fixatives and should be considered when the usual stains are not satisfactor
