標題: Titlebook: Eicosanoid Protocols; Elias A. Lianos Book 1999 Humana Press 1999 [打印本頁] 作者: 無法修復 時間: 2025-3-21 19:40
書目名稱Eicosanoid Protocols影響因子(影響力)
書目名稱Eicosanoid Protocols影響因子(影響力)學科排名
書目名稱Eicosanoid Protocols網絡公開度
書目名稱Eicosanoid Protocols網絡公開度學科排名
書目名稱Eicosanoid Protocols被引頻次
書目名稱Eicosanoid Protocols被引頻次學科排名
書目名稱Eicosanoid Protocols年度引用
書目名稱Eicosanoid Protocols年度引用學科排名
書目名稱Eicosanoid Protocols讀者反饋
書目名稱Eicosanoid Protocols讀者反饋學科排名
作者: 忍耐 時間: 2025-3-21 21:26
1064-3745 g prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of establis作者: SLING 時間: 2025-3-22 02:15
https://doi.org/10.1007/978-3-658-11446-6 we and others reported the cloning of a second isoform of cyclooxygenase, now known as COX-2, that is rapidly induced in response to growth factors and proinflammatory cytokines and downregulated by glucocorticoid hormones (.–.).作者: 沒收 時間: 2025-3-22 07:50
https://doi.org/10.1007/978-1-4842-1842-6t decade, several mammalian PLA2s have been shown to play a role in the mobilization of AA from phospholipids. At least ten different PLA. types have been described (.,.). In general, these consist of secretory, cytosolic, or membrane bound activities.作者: folliculitis 時間: 2025-3-22 11:31
https://doi.org/10.1007/978-1-4842-3643-7resis apparatus. Sample proteins are solubilized by boiling in the presence of SDS and equal amounts of the protein solution are loaded into a gel lane, and the individual proteins separated electrophoretically. 2-Mercaptoethanol (2-ME) and dithiothreitol (DTT) are added to reduce disulfide bonds.作者: Interferons 時間: 2025-3-22 13:58
Patrick Devanthéry,Inès Lamunièretis, Arthus pleurisy, and flares of colitis; research aimed at the molecular attachments between vascular endothelial cells (.) and transendothelial leukocyte migration (.–.) is a promising strategy for discovery of anti-inflammatory medications.作者: Interferons 時間: 2025-3-22 20:58 作者: 褪色 時間: 2025-3-23 01:10 作者: generic 時間: 2025-3-23 02:47 作者: ASTER 時間: 2025-3-23 05:58 作者: 闡釋 時間: 2025-3-23 10:13
https://doi.org/10.1007/978-3-658-15040-2 obtain stable expression of the exogenous gene. Viral vectors, including retroviruses, adenoviruses, and the Sinbis virus have been developed over the last decade for foreign gene expression in mammalian cells. This chapter will discuss the use of a replication-incompetent retroviral vector in cell culture to express an exogenous protein.作者: 都相信我的話 時間: 2025-3-23 14:07
https://doi.org/10.1007/978-1-4302-4570-4n mast cells and neutrophils, the major subclasses of PC are 1-acyl- and 1-alkyl-linked, whereas 1-acyl- and 1-alk-1-enyl-linked subclasses constitute the bulk of arachidonate in PE (.–.). PI is composed mainly of 1-acyl-linked species in these cells.作者: 從屬 時間: 2025-3-23 20:23
https://doi.org/10.1007/978-1-4842-3688-8wn concentration of a labeled eicosanoid with an unknown quantity of the same unlabeled eicosanoid. The antibody used has been generated against the specific eicosanoid to be measured. EIA is similar to RIA as an assay except that instead of using a radiolabeled compound, the competing antigen is labeled with a specific enzyme.作者: TEN 時間: 2025-3-24 02:14 作者: fertilizer 時間: 2025-3-24 04:20 作者: 善于騙人 時間: 2025-3-24 07:29 作者: 婚姻生活 時間: 2025-3-24 11:12 作者: lanugo 時間: 2025-3-24 17:50
Enzyme immunoassays (EIA) of Eicosanoids,wn concentration of a labeled eicosanoid with an unknown quantity of the same unlabeled eicosanoid. The antibody used has been generated against the specific eicosanoid to be measured. EIA is similar to RIA as an assay except that instead of using a radiolabeled compound, the competing antigen is labeled with a specific enzyme.作者: TAP 時間: 2025-3-24 19:16
Transcellular Regulation of Eicosanoid Biosynthesis,s, lipid mediators, nitric oxide, and chemoattractants. It thus has become apparent that transcellular eicosanoid biosynthesis during these interactions is an important means to amplify production of and/or results in the formation of novel mediators that neither cell type can generate alone (.–.).作者: Ingratiate 時間: 2025-3-24 23:36
Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts,es by appropriate stimuli. This chapter describes methods used for the detection of PGS2 expression by western and northern analyses in murine Swiss 3T3 fibroblasts. These methods have also been used successfully to detect PGS2 expression in a number of other murine cell lines (.).作者: 切割 時間: 2025-3-25 06:13
Book 1999novel eicosanoids..Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation.Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function ofeicosanoids. ....作者: neolith 時間: 2025-3-25 09:27 作者: 為敵 時間: 2025-3-25 13:39
https://doi.org/10.1007/978-3-658-31961-8 be separated, detected, and quantitated by several analytical techniques, including immunoassay, bioassay, gas chromatography-mass spectrometry (GC-MS), thin-layer chromatography (TLC), ultraviolet spectroscopy, and high pressure liquid chromatography (HPLC).作者: 思考 時間: 2025-3-25 17:03
Assessment of Cyclooxygenase RNA Expression by Northern Hybridization,ntrifuged, yielding an upper aqueous phase containing RNA, while the lower organic phase and interphase contains protein and DNA. Following isopropanol precipitation, the RNA pellet is redissolved in a denaturing solution, reprecipitated with isopropanol, and washed with 75% ethanol.作者: 隨意 時間: 2025-3-25 22:01 作者: 構想 時間: 2025-3-26 03:31 作者: 同義聯想法 時間: 2025-3-26 07:47
https://doi.org/10.1007/978-3-658-15040-2g the proteins involved in the control of eicosanoid production. Many transient expression vectors are commercially available and are in widespread use. However, some cell types, such as the renal mesangial cell, have been difficult to transform with these vectors and require a different strategy to作者: 拘留 時間: 2025-3-26 12:21 作者: angiography 時間: 2025-3-26 16:12 作者: 紳士 時間: 2025-3-26 19:29
Privatisierung und politische Macht variety of methods exist for immunocytochemical analysis including: immunofluorescence, immunoperoxidase, and streptavidin—biotin linked methods. Furthermore, both paraffin embedded and frozen tissue samples can be utilized for immunohistologic analysis.作者: 一瞥 時間: 2025-3-26 21:04
https://doi.org/10.1007/978-3-658-31961-8s (HETEs), leukotrienes (LTs), lipoxins (Lxs), epoxy metabolites, and hepoxilins (.). Most of these compounds are formed in minute quantities in response to specific stimuli and participate in modulation of the vascular tone or of the inflammatory process. We have been studying the formation of arac作者: 物質 時間: 2025-3-27 02:10 作者: 支形吊燈 時間: 2025-3-27 08:50 作者: infinite 時間: 2025-3-27 10:41
https://doi.org/10.1007/978-1-4302-4570-4he major eicosanoids include leukotrienes, prostaglandins, and thromboxanes. In inflammatory cells, the bulk of AA is esterified into phospholipids (PE, PC, and PI/PS). Arachidonate is further localized in specific subclasses based on different . substituents of the glycerol backbone of PC and PE. I作者: Adenoma 時間: 2025-3-27 16:19 作者: 推崇 時間: 2025-3-27 20:30
https://doi.org/10.1007/978-1-4842-3688-8um), tissue, urine, or culture media. Both radioimmunoassay and enzyme immunoassay techniques for the measurement of eicosanoids are based on the principles of the competitive substrate binding assay. More specifically, these assays involve the competition (for binding sites on an antibody) of a kno作者: 榮幸 時間: 2025-3-27 23:12 作者: TERRA 時間: 2025-3-28 04:48 作者: 單片眼鏡 時間: 2025-3-28 06:24 作者: 真 時間: 2025-3-28 11:15
Integrating DBA and DevOps Processes,ivity of phospholipases is converted to PGH2, a common precursor of prostaglandins, by the enzymatic activity of prostaglandin synthase (PGS). There are two forms of PGS enzymes; PGS1 and PGS2. PGS1 is constitutively expressed in almost all cell types, whereas PGS2 is induced in a number of cell typ作者: 披肩 時間: 2025-3-28 15:47
Securing Build Systems for DevOps,cells involves redistribution of 5-LO from a soluble cellular compartment where it is inactive to the nuclear envelope where its association with an activating protein, termed FLAP, is required for activation and optimal catalysis (.). The mechanism and extent of this redistribution and activation v作者: 山間窄路 時間: 2025-3-28 20:45 作者: 雄辯 時間: 2025-3-28 23:38
A Gentle Introduction to ML and NLP,studies. In numerous investigations of antiplatelet agents, the capacity of blood to generate thromboxane A. (TXA.) in response to an agonist has been assessed, and similar assays figure importantly in the current clinical trials (.) of cyclooxygenase-2 (COX-2) inhibitors. Early work by Sun (.), car作者: Kidnap 時間: 2025-3-29 03:36 作者: indubitable 時間: 2025-3-29 07:26
Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin, variety of methods exist for immunocytochemical analysis including: immunofluorescence, immunoperoxidase, and streptavidin—biotin linked methods. Furthermore, both paraffin embedded and frozen tissue samples can be utilized for immunohistologic analysis.作者: FLUSH 時間: 2025-3-29 12:01
Novel Eicosanoids,e IsoPs have been published recently (.–.). Therefore, many aspects of this subject will only be summarized briefly for sake of completeness. Rather, in this chapter, we have provided an update on previously published reviews by focusing in more detail on new information and new emerging areas related to the IsoPs.作者: Interdict 時間: 2025-3-29 15:47 作者: Type-1-Diabetes 時間: 2025-3-29 19:58
Privatisierung und politische Macht variety of methods exist for immunocytochemical analysis including: immunofluorescence, immunoperoxidase, and streptavidin—biotin linked methods. Furthermore, both paraffin embedded and frozen tissue samples can be utilized for immunohistologic analysis.作者: CLAM 時間: 2025-3-30 01:00
Developing with Unity and Visual Studioe IsoPs have been published recently (.–.). Therefore, many aspects of this subject will only be summarized briefly for sake of completeness. Rather, in this chapter, we have provided an update on previously published reviews by focusing in more detail on new information and new emerging areas related to the IsoPs.作者: 不能根除 時間: 2025-3-30 05:54
Use of a Retroviral Vector for Expression of a Foreign Gene,g the proteins involved in the control of eicosanoid production. Many transient expression vectors are commercially available and are in widespread use. However, some cell types, such as the renal mesangial cell, have been difficult to transform with these vectors and require a different strategy to作者: 教義 時間: 2025-3-30 09:58 作者: LASH 時間: 2025-3-30 12:51 作者: 有法律效應 時間: 2025-3-30 17:25 作者: ORE 時間: 2025-3-30 22:02 作者: 啜泣 時間: 2025-3-31 01:55
Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry,he first steps in the conversion from arachidonic acid. A cDNA for cyclooxygenase was first cloned from sheep seminal vesicles in 1988 (.,.). In 1991, we and others reported the cloning of a second isoform of cyclooxygenase, now known as COX-2, that is rapidly induced in response to growth factors a作者: Seizure 時間: 2025-3-31 07:53
Assessment of Cyclooxygenase Protein Expression by Western Blotting, an unknown complex protein sample. Electrophoresis is used to separate complex mixtures of proteins. Denaturing discontinuous one-dimensional gel electrophoresis separates proteins only based on molecular size as they move through a SDS-polyacrylamide gel (SDS-PAGE) toward the anode with the smalle作者: Pamphlet 時間: 2025-3-31 11:06 作者: 草率男 時間: 2025-3-31 17:06 作者: Canyon 時間: 2025-3-31 17:35
Enzyme immunoassays (EIA) of Eicosanoids,um), tissue, urine, or culture media. Both radioimmunoassay and enzyme immunoassay techniques for the measurement of eicosanoids are based on the principles of the competitive substrate binding assay. More specifically, these assays involve the competition (for binding sites on an antibody) of a kno作者: PAD416 時間: 2025-3-31 22:48
Transcellular Regulation of Eicosanoid Biosynthesis,ts that are generated by individual cell types in vitro. In vivo scenarios encountered in inflammation, thrombosis, atherosclerosis, asthma, and other multicellular events encompass an array of cell–cell interactions as well as a complex interplay of factors that include adhesion molecules, cytokine作者: sclera 時間: 2025-4-1 05:01 作者: 駕駛 時間: 2025-4-1 06:06
