標(biāo)題: Titlebook: Ebolaviruses; Methods and Protocol Thomas Hoenen,Allison Groseth Book 2017 Springer Science+Business Media LLC 2017 Ebola virus.Virology.Za [打印本頁] 作者: Helmet 時(shí)間: 2025-3-21 20:06
書目名稱Ebolaviruses影響因子(影響力)
書目名稱Ebolaviruses影響因子(影響力)學(xué)科排名
書目名稱Ebolaviruses網(wǎng)絡(luò)公開度
書目名稱Ebolaviruses網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Ebolaviruses被引頻次
書目名稱Ebolaviruses被引頻次學(xué)科排名
書目名稱Ebolaviruses年度引用
書目名稱Ebolaviruses年度引用學(xué)科排名
書目名稱Ebolaviruses讀者反饋
書目名稱Ebolaviruses讀者反饋學(xué)科排名
作者: 不能根除 時(shí)間: 2025-3-21 22:47 作者: 半身雕像 時(shí)間: 2025-3-22 01:19 作者: MILL 時(shí)間: 2025-3-22 08:04 作者: indigenous 時(shí)間: 2025-3-22 10:55 作者: 薄荷醇 時(shí)間: 2025-3-22 14:23 作者: 薄荷醇 時(shí)間: 2025-3-22 19:48
1064-3745 implementation advice from the experts.Includes supplementarThis vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and fo作者: 彩色的蠟筆 時(shí)間: 2025-3-23 01:05 作者: BET 時(shí)間: 2025-3-23 03:13
Modeling Ebolavirus Budding with Virus Like ParticlesP40 residues involved in this process. VLPs are now produced routinely in several laboratories as a tool to study virus entry or egress and have even been proposed as vaccine candidates against Ebola virus disease. Here we described protocols for the production and the analysis of Ebola virus VLP release.作者: SEED 時(shí)間: 2025-3-23 07:29
Treatment of Advanced-Stage Ovarian Cancer,disease features. We also describe the current animal models used in ebolavirus research, detailing each model’s unique strengths and weaknesses. We focus on Ebola virus representing the type species . of the genus ., as most work relates to this pathogen.作者: 討厭 時(shí)間: 2025-3-23 11:16 作者: 代理人 時(shí)間: 2025-3-23 14:30
Advances in Distillation Retrofitnize RLR signaling. Ebola virus (EBOV) and other filoviruses produce VP35 proteins that block IFN induction via RLR signaling. We describe here cell-based reporter gene assays that quantify the IFN-antagonist function of filovirus VP35 proteins by assessing activation of the IFN-β promoter.作者: bypass 時(shí)間: 2025-3-23 18:39 作者: 其他 時(shí)間: 2025-3-24 01:59
Book 2017ancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious eb作者: 失望未來 時(shí)間: 2025-3-24 05:57 作者: 丑惡 時(shí)間: 2025-3-24 07:33 作者: wangle 時(shí)間: 2025-3-24 12:25 作者: cloture 時(shí)間: 2025-3-24 17:41 作者: 音樂會(huì) 時(shí)間: 2025-3-24 19:13 作者: HEW 時(shí)間: 2025-3-25 01:56
https://doi.org/10.1007/0-306-47914-1rect study of .-acting regulatory regions as well as .-acting factors involved in Ebola virus minigenome transcription compared to the indirect measurement of reporter protein activity that additionally reflects translational effects (. Chapter . in this book for details).作者: 免除責(zé)任 時(shí)間: 2025-3-25 07:00
Navin C. Nanda,Joel S. Raichlention of tagged and particularly fluorescent or luminescent viruses can be applied as tools for both diagnostic applications and for screening to identify novel countermeasures. Here we describe the generation and basic characterization of recombinant Ebola viruses rescued from cloned cDNA using a T7-driven system.作者: 實(shí)現(xiàn) 時(shí)間: 2025-3-25 08:29 作者: 項(xiàng)目 時(shí)間: 2025-3-25 11:53
Forty Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent Through the Developh, if not driven by, advancements in the technical methodologies available for these kinds of studies. Here we review of the current state of our knowledge regarding ebolavirus biology and emphasize the associated methods that made these discoveries possible.作者: 吼叫 時(shí)間: 2025-3-25 19:18
Nonradioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNArect study of .-acting regulatory regions as well as .-acting factors involved in Ebola virus minigenome transcription compared to the indirect measurement of reporter protein activity that additionally reflects translational effects (. Chapter . in this book for details).作者: 宣誓書 時(shí)間: 2025-3-25 23:23
Generation of Recombinant Ebola Viruses Using Reverse Geneticstion of tagged and particularly fluorescent or luminescent viruses can be applied as tools for both diagnostic applications and for screening to identify novel countermeasures. Here we describe the generation and basic characterization of recombinant Ebola viruses rescued from cloned cDNA using a T7-driven system.作者: 加花粗鄙人 時(shí)間: 2025-3-26 02:52
Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals short assay times. Here we provide a detailed protocol for the use of such a luciferase-expressing reporter virus for antiviral screening in a 96-well format, with parallel assessment of cytotoxicity of the screened compounds.作者: Minutes 時(shí)間: 2025-3-26 05:20 作者: 騙子 時(shí)間: 2025-3-26 08:38 作者: extinct 時(shí)間: 2025-3-26 15:25 作者: Reservation 時(shí)間: 2025-3-26 19:42
A Semi-automated High-Throughput Microtitration Assay for Filovirusesng a luminescent-based cell viability assay and an automated plate reader, has improved workflow efficiency, reduced operator burden and assay time, decreased assay variability, and increased data return.作者: 替代品 時(shí)間: 2025-3-26 22:53 作者: 智力高 時(shí)間: 2025-3-27 03:54 作者: Negligible 時(shí)間: 2025-3-27 07:16
J. Bleyer,D. T. Pham,P. de Buhand to assess protein-RNA complexes. Here we describe an adapted RNA immunoprecipitation technique that permits the quantification of RNA content in Ebola virus nucleocapsids that have been reconstituted in vitro by transient transfection.作者: 引起 時(shí)間: 2025-3-27 11:53
Marburg- and Ebolaviruses: A Look Back and Lessons for the Futures as dramatic as most of the subsequent ones, but none of them was as devastating as the West-African Ebola virus outbreak in 2013–2015. Although progress toward a better understanding of the viruses is impressive, there is clearly a need to improve and strengthen the measures to detect and control these deadly infections.作者: OATH 時(shí)間: 2025-3-27 16:03
Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitationd to assess protein-RNA complexes. Here we describe an adapted RNA immunoprecipitation technique that permits the quantification of RNA content in Ebola virus nucleocapsids that have been reconstituted in vitro by transient transfection.作者: 痛打 時(shí)間: 2025-3-27 21:39
Ren-You Pan,Chun-Bing Chen,Wen-Hung Chungs as dramatic as most of the subsequent ones, but none of them was as devastating as the West-African Ebola virus outbreak in 2013–2015. Although progress toward a better understanding of the viruses is impressive, there is clearly a need to improve and strengthen the measures to detect and control 作者: delusion 時(shí)間: 2025-3-27 22:52 作者: 和平 時(shí)間: 2025-3-28 05:42 作者: Ornithologist 時(shí)間: 2025-3-28 07:01 作者: 不能逃避 時(shí)間: 2025-3-28 12:20 作者: archenemy 時(shí)間: 2025-3-28 15:05
J. Bleyer,D. T. Pham,P. de Buhand to assess protein-RNA complexes. Here we describe an adapted RNA immunoprecipitation technique that permits the quantification of RNA content in Ebola virus nucleocapsids that have been reconstituted in vitro by transient transfection.作者: 破布 時(shí)間: 2025-3-28 20:02
Probabilistic Recurrence Relations,ls. Further development of the protocol later allowed for the dissection of the Ebola virus budding mechanism and for the identification of critical VP40 residues involved in this process. VLPs are now produced routinely in several laboratories as a tool to study virus entry or egress and have even 作者: 招待 時(shí)間: 2025-3-28 23:26
Raphael Boll,Matteo Petrera,Yuri B. Suris As biosafety level 4 (BSL4) agents, work with live ebolaviruses is restricted to maximum containment laboratories. Transcription and replication-competent viruslike particle (trVLP) systems are reverse genetics-based life cycle modeling systems that allow researchers to model virtually the entire e作者: 我沒有命令 時(shí)間: 2025-3-29 05:32
Advances in Distillation Retrofithat includes IFN-β and several IFN-αs. For the filoviruses and many other RNA viruses that replicate in the cytoplasm, the RIG-I-like pattern recognition receptors (RLRs) are potential triggers of IFN production. To counteract such innate antiviral responses, many viruses encode proteins that antago作者: 浮夸 時(shí)間: 2025-3-29 10:34 作者: 消息靈通 時(shí)間: 2025-3-29 12:37 作者: 沒有貧窮 時(shí)間: 2025-3-29 19:33
Navin C. Nanda,Joel S. Raichlenl-length clone systems allow us to use plasmid-encoded versions of the ribonucleoprotein (RNP) components to initiate the transcription and replication of a plasmid-encoded version of the complete viral genome, thereby initiating the complete virus life cycle and resulting in infectious virus. As su作者: 優(yōu)雅 時(shí)間: 2025-3-29 20:08 作者: 織物 時(shí)間: 2025-3-30 00:04 作者: BOOM 時(shí)間: 2025-3-30 04:52
Advances in Economic Measurementity and should presumably be further investigated in animal efficacy trials. However, despite the many compounds that are purported to have good antiviral activity in in vitro studies, there are few instances where any efficacy has been reported in nonhuman primate models. Many of the high-throughpu作者: 頌揚(yáng)本人 時(shí)間: 2025-3-30 09:49 作者: Adj異類的 時(shí)間: 2025-3-30 15:14
Modeling Ebola Virus Genome Replication and Transcription with Minigenome SystemsIn this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.作者: 負(fù)擔(dān) 時(shí)間: 2025-3-30 17:21 作者: 殘忍 時(shí)間: 2025-3-30 21:44
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/301062.jpg作者: chassis 時(shí)間: 2025-3-31 03:58
https://doi.org/10.1007/978-1-4939-7116-9Ebola virus; Virology; Zaire ebolavirus; Biosafety levels; Filoviruses; Immune response; infectious diseas作者: CULP 時(shí)間: 2025-3-31 08:08 作者: 復(fù)習(xí) 時(shí)間: 2025-3-31 10:32
Marburg- and Ebolaviruses: A Look Back and Lessons for the Futures as dramatic as most of the subsequent ones, but none of them was as devastating as the West-African Ebola virus outbreak in 2013–2015. Although progress toward a better understanding of the viruses is impressive, there is clearly a need to improve and strengthen the measures to detect and control 作者: 肥料 時(shí)間: 2025-3-31 14:26
Forty Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent Through the Develop, RNA, proteins, and/or other macromolecules (e.g., those involved in the modification of these substrates). Consequently, it relies on the availability of a wide variety of methods that deal with the collection, preservation, inactivation, separation, manipulation, imaging, and analysis of these mo
