標題: Titlebook: ERK Signaling; Methods and Protocol Gerardo Jimenez Book 2017 Springer Science+Business Media New York 2017 signaling pathways.ERK2 protein [打印本頁] 作者: 故障 時間: 2025-3-21 18:40
書目名稱ERK Signaling影響因子(影響力)
書目名稱ERK Signaling影響因子(影響力)學科排名
書目名稱ERK Signaling網(wǎng)絡(luò)公開度
書目名稱ERK Signaling網(wǎng)絡(luò)公開度學科排名
書目名稱ERK Signaling被引頻次
書目名稱ERK Signaling被引頻次學科排名
書目名稱ERK Signaling年度引用
書目名稱ERK Signaling年度引用學科排名
書目名稱ERK Signaling讀者反饋
書目名稱ERK Signaling讀者反饋學科排名
作者: Gentry 時間: 2025-3-21 22:47 作者: hegemony 時間: 2025-3-22 02:21
e human epidermal growth factor (EGF) and the human EGF receptor (EGFR) fused with a signal peptide at the N terminus facilitated the interaction of EGF with EGFR in an autocrine manner, followed by EGFR oligomerization and subsequent autophosphorylation. Furthermore, yeast cells expressing cell-wal作者: Classify 時間: 2025-3-22 05:55 作者: 把…比做 時間: 2025-3-22 10:52
, generating a library of radiolabeled protein pools which are subsequently subjected to biochemical kinase assays using recombinant, active Erk2. Phosphorylated proteins representing potential MAPK/Erk substrates are then detected due to their shifted mobility on SDS-PAGE gels. This protocol can be作者: 保守黨 時間: 2025-3-22 14:28 作者: 保守黨 時間: 2025-3-22 18:12 作者: 香料 時間: 2025-3-22 21:51
https://doi.org/10.1007/978-3-476-99966-5equently promotes their translocation to the nucleus. More studies are still required in order to better understand the mechanism and consequence of the nuclear translocation of ERK1/2. In this chapter, we describe some of the techniques used to study nuclear translocation of ERK1/2 in mammalian cel作者: intangibility 時間: 2025-3-23 02:44 作者: GLEAN 時間: 2025-3-23 08:00 作者: 弓箭 時間: 2025-3-23 14:21
Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE,D-DIGE gels. Validation experiments such as Phos-tag Western blotting are important steps to further elucidate the functional roles of ERK-mediated phosphorylation of these newly identified substrates.作者: 返老還童 時間: 2025-3-23 20:45
Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation,function mainly as transcription factors. The other half resides in the cytosol and other cellular organelles. Such subcellular distribution enhances the complexity of the Ras/ERK cascade and constitutes an essential mechanism to endow variability to its signals, which enables their participation in作者: 一大群 時間: 2025-3-24 00:33
The Nuclear Translocation of ERK,equently promotes their translocation to the nucleus. More studies are still required in order to better understand the mechanism and consequence of the nuclear translocation of ERK1/2. In this chapter, we describe some of the techniques used to study nuclear translocation of ERK1/2 in mammalian cel作者: hemophilia 時間: 2025-3-24 03:50
Book 2017 molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and ERK activities; proteomic and genome-wide analyses of ERK signaling targets; cell biological, genetic, quantitative and imaging approaches in cells an作者: 必死 時間: 2025-3-24 07:48
1064-3745 tion advice from the experts.This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including 作者: 鋼筆記下懲罰 時間: 2025-3-24 13:26 作者: Barrister 時間: 2025-3-24 15:22 作者: 極力證明 時間: 2025-3-24 19:40
In Vitro Enzyme Kinetics Analysis of EGFR,s a detailed protocol to purify and characterize full-length EGFR bound with EGF. This protocol facilitates the expression and preparation of full-length EGFR in transiently transfected mammalian cell culture. A method is also presented for quantitative evaluation of EGFR enzyme activity.作者: archetype 時間: 2025-3-25 01:39
Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptident isolation of native ERK signaling complexes, which are suitable for subsequent analysis by mass spectrometry. Our analysis of the ERK interactome has identified both known and novel signaling components. This method can be easily adapted for SBP-based purification of protein complexes in any expression system.作者: 尊嚴 時間: 2025-3-25 04:06 作者: Harpoon 時間: 2025-3-25 08:29 作者: MELD 時間: 2025-3-25 14:00
Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors,dynamic datasets. In this chapter, we describe the analysis of endogenous extracellular signal-regulated kinase (ERK) activity in living cells using the EKAR2G (ERK activity reporter second generation) probe. We focus on the generation of stable cell lines expressing the EKAR2G sensor as well as data acquisition and analysis.作者: 假裝是我 時間: 2025-3-25 19:42 作者: acetylcholine 時間: 2025-3-25 21:18 作者: 完成才能戰(zhàn)勝 時間: 2025-3-26 01:56
https://doi.org/10.1007/978-3-322-80760-1nt isolation of native ERK signaling complexes, which are suitable for subsequent analysis by mass spectrometry. Our analysis of the ERK interactome has identified both known and novel signaling components. This method can be easily adapted for SBP-based purification of protein complexes in any expression system.作者: 自傳 時間: 2025-3-26 06:24
https://doi.org/10.1007/978-3-476-99011-2p between actin tension and ERK phosphorylation is missing. In this chapter, we describe our novel methods to quantify tensile force and ERK phosphorylation on individual actin stress fibers. These methods have enabled us to show that ERK is activated on stress fibers in a tensile force-dependent manner.作者: modish 時間: 2025-3-26 10:05
https://doi.org/10.1007/978-3-642-51143-1newal and early lineage commitment. In this chapter, we describe methods used for the isolation and establishment of mouse ES cell lines from blastocyst embryos and for the measurement of ERK1/2 activity in ES cells.作者: 遵循的規(guī)范 時間: 2025-3-26 16:14 作者: Vital-Signs 時間: 2025-3-26 16:57 作者: ABYSS 時間: 2025-3-27 00:01 作者: 老人病學 時間: 2025-3-27 05:06
https://doi.org/10.1007/BFb0077472 an air–liquid interface to enable diffusion and nourishment from the medium below. Subsequently, the organotypic cultures can be used for immunohistochemical staining of various components of ERK signaling, which is a key player in mediating communication between cells and their microenvironment.作者: botany 時間: 2025-3-27 07:42 作者: DEFER 時間: 2025-3-27 12:50
Assaying Activation and Subcellular Localization of ERK in Cells and Tissues, we describe methods to assay ERK activity based on the ability of ERK immunocomplexes to phosphorylate a substrate, as well as on immunoblot analysis using antibodies that recognize ERK1/2 phosphorylated in the Thr-X-Tyr motif. In addition, we describe an immunocytochemistry procedure to reveal stimuli-induced nuclear translocation of ERK1/2.作者: 發(fā)酵 時間: 2025-3-27 14:34
Detection and Functional Analysis of SUMO-Modified MEK,escribe several tools and techniques utilized for the elucidation of the properties of SUMO-MEK in our previous reports. We believe that these methods can be used as robust tools for investigating and understanding the biological roles of various SUMO-modified (sumoylated) proteins.作者: 出處 時間: 2025-3-27 17:52
3D Organotypic Culture Model to Study Components of ERK Signaling, an air–liquid interface to enable diffusion and nourishment from the medium below. Subsequently, the organotypic cultures can be used for immunohistochemical staining of various components of ERK signaling, which is a key player in mediating communication between cells and their microenvironment.作者: Individual 時間: 2025-3-27 22:23
https://doi.org/10.1007/978-981-19-3551-0 ERK2 activation loop in various different phosphorylation states. Here we describe the methods used to derive crystallization-grade complexes of ERK2-PEA-15, which may also be adapted for other regulators that associate with the activation loop of ERK1/2.作者: Genteel 時間: 2025-3-28 02:46 作者: 名詞 時間: 2025-3-28 09:28
How Genetics Has Helped Piece Together the MAPK Signaling Pathway,These networks can be decomposed into a multitude of signaling pathways that relay signals from the microenvironment to the cellular components involved in eliciting a specific response. Perturbations in these signaling processes are at the root of multiple pathologies, the most notable of these bei作者: muscle-fibers 時間: 2025-3-28 12:33 作者: temperate 時間: 2025-3-28 15:39 作者: N防腐劑 時間: 2025-3-28 20:07
Structural Studies of ERK2 Protein Complexes,ciate with ERK1/2 through distinct D-peptide- and DEF-docking sites on their kinase domains. While understanding of D-peptides that bind to ERK1/2 has become increasingly clear over the last decade, only more recently have structures of proteins interacting with other binding sites on ERK1/2 become 作者: Carbon-Monoxide 時間: 2025-3-29 00:17 作者: 小木槌 時間: 2025-3-29 04:48
Assaying Activation and Subcellular Localization of ERK in Cells and Tissues,nd pathological processes, such as cell proliferation and differentiation, and oncogenic transformation, respectively. ERK1/2 belong to the mitogen-activated protein kinase (MAPKs) family, which are serine/threonine kinases that participate in signal transduction and are activated by dual phosphoryl作者: Chagrin 時間: 2025-3-29 10:08
Detection and Functional Analysis of SUMO-Modified MEK,y alters their functions. Protein sumoylation has been linked to various cellular functions such as cell division, DNA repair, and import of nuclear proteins. Thus, its dysregulation is implicated in diverse human diseases such as neurodegenerative disorders and cancers. We recently found that the k作者: 鞠躬 時間: 2025-3-29 15:04
Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide combined with mass spectrometry (AP-MS) has become a favorite method to study protein complexes. Here we describe a procedure for single-step purification of ERK (Rolled) and associated proteins from . cultured cells. The use of the streptavidin-binding peptide (SBP) tag allows for a highly efficie作者: Demulcent 時間: 2025-3-29 16:35 作者: Gratulate 時間: 2025-3-29 20:24
Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE, not yet been fully characterized. To date, several phosphoproteomic approaches have been employed to identify novel substrates for ERK. In this chapter, we describe a method to globally identify ERK substrates by combining immobilized metal affinity chromatography (IMAC) and two-dimensional differe作者: 漂亮才會豪華 時間: 2025-3-30 02:36
Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation,lular functions, including key processes such as proliferation, cell cycle progression, differentiation, and survival. The duration, intensity and specificity of the responses are, in part, controlled by the compartmentalization/subcellular localization of the signaling intermediaries. Ras proteins 作者: 類型 時間: 2025-3-30 05:47 作者: anatomical 時間: 2025-3-30 10:44 作者: acheon 時間: 2025-3-30 12:57
Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA),ties in RAS/MAPK signaling cascade have been intimately implicated in numerous human diseases, including cancer. Herein, we describe a novel methodology to study activation of this pivotal signaling pathway. The Proximity Ligation Assay (PLA) is employed to monitor kinase–substrate interactions betw作者: textile 時間: 2025-3-30 18:49
Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors,lls with subcellular resolution. There are quite a number of already existing sensors and this technology is increasingly used to obtain quantitative dynamic datasets. In this chapter, we describe the analysis of endogenous extracellular signal-regulated kinase (ERK) activity in living cells using t作者: 頌揚國家 時間: 2025-3-30 22:01 作者: 無聊的人 時間: 2025-3-31 01:47
Co-culture Activation of MAP Kinase in , S2 Cells,ological detection of phosphorylated MAPK can be used to monitor signaling in vivo, identify novel pathway components, and assess ligand activity. In this chapter, I describe a cell co-culture method to assess activity of cell-bound extracellular ligands that result in phosphorylation of the ERK (ex作者: pacific 時間: 2025-3-31 05:07 作者: 費解 時間: 2025-3-31 10:34 作者: Commemorate 時間: 2025-3-31 13:55 作者: 蜈蚣 時間: 2025-3-31 18:37
978-1-4939-8195-3Springer Science+Business Media New York 2017作者: cornucopia 時間: 2025-4-1 01:02
Gerardo JimenezIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts作者: 表否定 時間: 2025-4-1 04:37
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/300482.jpg作者: monochromatic 時間: 2025-4-1 07:42 作者: 殘暴 時間: 2025-4-1 13:06 作者: PARA 時間: 2025-4-1 17:02
ERK Signaling978-1-4939-6424-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 帶來的感覺 時間: 2025-4-1 18:57
https://doi.org/10.1007/978-3-476-04152-4many membrane receptors. Here, we summarize different techniques set up to study changes in cell morphology and cell motility regulated by ERK/caveolin1 interactions during induction of epithelial mesenchymal transition (EMT) in mesothelial cells (MCs).作者: 誘騙 時間: 2025-4-2 02:26
https://doi.org/10.1007/978-3-476-99761-6ties in RAS/MAPK signaling cascade have been intimately implicated in numerous human diseases, including cancer. Herein, we describe a novel methodology to study activation of this pivotal signaling pathway. The Proximity Ligation Assay (PLA) is employed to monitor kinase–substrate interactions between MEK1 and HSF1, or MEK1 and ERK1 in situ.作者: 擁擠前 時間: 2025-4-2 05:55
