標題: Titlebook: ELISA; Methods and Protocol Robert Hnasko Book 2015 Springer Science+Business Media New York 2015 Enzyme-Linked Immunosorbent Assay.antibod [打印本頁] 作者: 時間 時間: 2025-3-21 18:45
書目名稱ELISA影響因子(影響力)
書目名稱ELISA影響因子(影響力)學科排名
書目名稱ELISA網(wǎng)絡公開度
書目名稱ELISA網(wǎng)絡公開度學科排名
書目名稱ELISA被引頻次
書目名稱ELISA被引頻次學科排名
書目名稱ELISA年度引用
書目名稱ELISA年度引用學科排名
書目名稱ELISA讀者反饋
書目名稱ELISA讀者反饋學科排名
作者: 共棲 時間: 2025-3-21 21:22 作者: 維持 時間: 2025-3-22 03:50
Affinity Purification of Antibodies,n crude form for the detection of target antigens by immunoassay. However, it is advantageous to use purified antibody in defined quantity to facilitate assay reproducibility, economy, and reduced interference of nonspecific components as well as improved storage, stability, and bio-conjugation. Alt作者: 孤僻 時間: 2025-3-22 05:07 作者: Goblet-Cells 時間: 2025-3-22 12:43
Indirect ELISA,d surface. Being one of the most sensitive immunoassays, ELISA offers commercial value in laboratory research, diagnostic of disease biomarkers, and quality control in various industries. This technique utilizes an enzyme-linked antibody binding to a surface-attached antigen. Subsequently, a substra作者: 黃瓜 時間: 2025-3-22 14:37
Direct ELISA,of an antigen using an enzyme-labeled antibody. Besides routine laboratory usage, ELISA has been utilized in medical field and food industry as diagnostic and quality control tools. Traditionally performed in 96-well or 384-well polystyrene plates, the technology has expanded to other platforms with作者: 黃瓜 時間: 2025-3-22 18:49 作者: 獨特性 時間: 2025-3-22 21:28
,ELISpot and DC-ELISpot Assay to Measure Frequency of Antigen-Specific IFNγ-Secreting Cells,d with a specific anti-analyte antibody. In response to the stimulation, cells release cytokines that are bound to the anti-analyte antibody. After a washing step, which removes the cells from the wells, the location of the cytokine-releasing cell is visualized by an enzyme-labeled detection antibod作者: 細微差別 時間: 2025-3-23 01:24 作者: MURAL 時間: 2025-3-23 05:39 作者: parasite 時間: 2025-3-23 10:49 作者: 陳腐的人 時間: 2025-3-23 14:50
,Multiplex Immunoassay: A Planar Array on a Chip Using the MagArray? Technology, to multiplex are often complex and expensive. Here, we describe a low-cost planar array on a chip capable of simultaneously detecting up to 80 different analytes using the MagArray technology in as little as 10 min. This technology is easy to operate, has a small footprint, and is highly portable a作者: 不發(fā)音 時間: 2025-3-23 18:49 作者: yohimbine 時間: 2025-3-23 23:03 作者: Delirium 時間: 2025-3-24 02:24
Tyramide Signal Amplification for Immunofluorescent Enhancement, Tyramide signal amplification (TSA) is based on a catalytic reporter deposit in close vicinity to the epitope of interest. The advantages of this technique are its simplicity, enhanced sensitivity, high specificity, and compatibility with modern multi-label fluorescent microscopy. Here, we describe作者: 固定某物 時間: 2025-3-24 10:24
Correlative Microscopy for Localization of Proteins In Situ: Pre-embedding Immuno-Electron Microscos are available for immuno-EM: pre-embedding method, post-embedding, and cryosectioning (Tokuyasu “style”). Here we describe a pre-embedding technique that allows the labeling of a target protein in situ. retention of fluorescence signal in plastic, and its localization at the EM level in a given ce作者: 刪減 時間: 2025-3-24 12:36 作者: Bravura 時間: 2025-3-24 18:41 作者: 喊叫 時間: 2025-3-24 21:21
1064-3745 ation advice from the experts.This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance proces作者: glucagon 時間: 2025-3-25 02:31 作者: artifice 時間: 2025-3-25 06:35 作者: 錯 時間: 2025-3-25 07:48 作者: 爆米花 時間: 2025-3-25 13:01 作者: Outspoken 時間: 2025-3-25 18:20
https://doi.org/10.1007/978-981-15-1408-1vides the procedures required for carrying out indirect ELISA, one of the many forms of ELISA, to detect polystyrene-immobilized antigen. Methodological approaches to optimize this assay technique are also described, a prerequisite for automation and multiplexing.作者: insincerity 時間: 2025-3-25 19:59
antibodies (mAbs) suitable for use as capture antibodies and detector antibodies in a sELISA targeting bacterial protein toxins. The approach was designed for early identification of monoclonal antibodies (mAbs), in the initial hybridoma screen.作者: 狂熱語言 時間: 2025-3-26 02:18 作者: 南極 時間: 2025-3-26 08:04
protein-A colloidal gold conjugate, which imparts a pink color. The test can be completed within 3 min at room temperature without any instrumentation. The sensitivity and specificity of the FTA are in agreement with ELISA.作者: 清晰 時間: 2025-3-26 09:18 作者: 瘋狂 時間: 2025-3-26 15:39
Affinity Purification of Antibodies,th basic chromatographic principles warrants purification when antibody source material is available in sufficient quantity. Here, we define three simple methods using immobilized (1) protein-A, (2) protein-G, and (3) protein-L agarose beads to yield highly purified antibody.作者: 失望昨天 時間: 2025-3-26 17:41
Indirect ELISA,vides the procedures required for carrying out indirect ELISA, one of the many forms of ELISA, to detect polystyrene-immobilized antigen. Methodological approaches to optimize this assay technique are also described, a prerequisite for automation and multiplexing.作者: 傲慢人 時間: 2025-3-27 00:38 作者: 要求比…更好 時間: 2025-3-27 04:47
,ELISpot and DC-ELISpot Assay to Measure Frequency of Antigen-Specific IFNγ-Secreting Cells,ytokine had been located..Here we describe the standard ELISpot protocol and a variation denominated dendritic cell (DC)-ELISpot for the detection of IFNγ-secreting cells upon stimulation with oligopeptides and protein antigens, respectively.作者: CANT 時間: 2025-3-27 08:49
Flow-Through Assay for Detection of Antibodies Using Protein-A Colloidal Gold Conjugate as a Probe,protein-A colloidal gold conjugate, which imparts a pink color. The test can be completed within 3 min at room temperature without any instrumentation. The sensitivity and specificity of the FTA are in agreement with ELISA.作者: DAFT 時間: 2025-3-27 12:04 作者: Synthesize 時間: 2025-3-27 15:24 作者: Seminar 時間: 2025-3-27 20:01
Multiplex ELISA Using Oligonucleotide Tethered Antibodies,umption, sample requirements and labor thereby lowering the cost per test. Here we describe a novel multiplex immunoassay technology based upon creating microarrays in microtiter plates that are formed upon the self-assembly of oligonucleotide–antibody conjugates.作者: GOAT 時間: 2025-3-27 22:18 作者: 小隔間 時間: 2025-3-28 02:38 作者: bioavailability 時間: 2025-3-28 07:28 作者: 詞匯表 時間: 2025-3-28 14:03
s that recognize the analyte of interest. As monoclonal antibody technology becomes more accessible to smaller laboratories, there has been increased interest in developing LFIA prototypes for potential commercial manufacture. In this chapter, the basics of designing and building an LFIA prototype are described.作者: exclamation 時間: 2025-3-28 16:50
https://doi.org/10.1007/978-3-319-66373-9mplification. The amplification power of the PCR allows for the detection of even single molecules of nucleic acid templates, making it well suited for a broad range of applications. Here, we describe the application of an IPCR assay for detection of trace amount of antigens using ricin as an example.作者: RAGE 時間: 2025-3-28 21:27 作者: Admire 時間: 2025-3-29 00:12 作者: 挫敗 時間: 2025-3-29 04:11
-throughput format. Luminex refers to this technology as xMAP.. Here we describe a routine protocol for a Luminex immunoassay. Other Luminex assays would have to be optimized for specific conditions according to their use.作者: Ventricle 時間: 2025-3-29 10:54 作者: URN 時間: 2025-3-29 13:26 作者: 中古 時間: 2025-3-29 18:04
Multiplexed Microsphere Suspension Array-Based Immunoassays,-throughput format. Luminex refers to this technology as xMAP.. Here we describe a routine protocol for a Luminex immunoassay. Other Luminex assays would have to be optimized for specific conditions according to their use.作者: delta-waves 時間: 2025-3-29 23:45
Tyramide Signal Amplification for Immunofluorescent Enhancement,ng with primary antibody, (4) binding with horseradish peroxidase-conjugated secondary antibody, (5) reacting with fluorescent tyramide substrate, and (6) imaging of the signal. The procedures described herein detail these steps and provide additional guidance and background to assist novice users.作者: 周年紀念日 時間: 2025-3-30 01:40 作者: 哄騙 時間: 2025-3-30 06:14 作者: Tdd526 時間: 2025-3-30 10:03
The Western Blot,ex mixture. This chapter will outline the requisite steps including gel electrophoresis of a protein sample, transfer of protein from a gel to a membrane support, and immunodetection of a target antigen.作者: alcohol-abuse 時間: 2025-3-30 13:24
Lateral Flow Immunoassay,s that recognize the analyte of interest. As monoclonal antibody technology becomes more accessible to smaller laboratories, there has been increased interest in developing LFIA prototypes for potential commercial manufacture. In this chapter, the basics of designing and building an LFIA prototype are described.作者: TEM 時間: 2025-3-30 19:15 作者: leniency 時間: 2025-3-30 22:13 作者: Malfunction 時間: 2025-3-31 03:18
Gas Plasma Surface Chemistry for Biological Assays,There are many commercial examples of plasma-modified products. These include well plates, filtration membranes, dispensing tools, and medical devices. This chapter presents an overview of gas plasma technology and provides a guide to using gas plasma for modifying surfaces for research or product development.作者: SPECT 時間: 2025-3-31 06:41
https://doi.org/10.1007/978-3-030-83324-4In situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols.作者: filial 時間: 2025-3-31 09:21
In Situ Proximity Ligation Assay (PLA),In situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols.作者: periodontitis 時間: 2025-3-31 14:56 作者: DEVIL 時間: 2025-3-31 20:58 作者: 小隔間 時間: 2025-4-1 00:41
https://doi.org/10.1007/978-3-663-02969-4Their antigen-binding properties define both the sensitivity and selectivity of an immunoassay. Understanding the biochemical properties of this class of protein will provide users with the knowledge necessary to select the appropriate antibody composition to maximize immunoassay results. Here we de作者: TERRA 時間: 2025-4-1 04:11 作者: 火海 時間: 2025-4-1 09:17
n crude form for the detection of target antigens by immunoassay. However, it is advantageous to use purified antibody in defined quantity to facilitate assay reproducibility, economy, and reduced interference of nonspecific components as well as improved storage, stability, and bio-conjugation. Alt作者: 包租車船 時間: 2025-4-1 12:07
tection of a target antigen can be achieved. The use of heterobifunctional cross-linkers to covalently link antibodies to HRP provides a simple and convenient means to maintain antibody affinity while imparting a functional reporter used for antigen detection. In this chapter, we describe a process 作者: Infiltrate 時間: 2025-4-1 17:38
https://doi.org/10.1007/978-981-15-1408-1d surface. Being one of the most sensitive immunoassays, ELISA offers commercial value in laboratory research, diagnostic of disease biomarkers, and quality control in various industries. This technique utilizes an enzyme-linked antibody binding to a surface-attached antigen. Subsequently, a substra作者: 慢慢流出 時間: 2025-4-1 19:06
Japan, Wirtschaftsmacht des fernen Ostensof an antigen using an enzyme-labeled antibody. Besides routine laboratory usage, ELISA has been utilized in medical field and food industry as diagnostic and quality control tools. Traditionally performed in 96-well or 384-well polystyrene plates, the technology has expanded to other platforms with作者: Mast-Cell 時間: 2025-4-1 22:59
