標(biāo)題: Titlebook: E. coli Plasmid Vectors; Methods and Applicat Nicola Casali,Andrew Preston Book 20031st edition Springer Science+Business Media New York 20 [打印本頁] 作者: TEMPO 時(shí)間: 2025-3-21 17:18
書目名稱E. coli Plasmid Vectors影響因子(影響力)
作者: 嫌惡 時(shí)間: 2025-3-21 22:59 作者: fetter 時(shí)間: 2025-3-22 02:28 作者: 獨(dú)行者 時(shí)間: 2025-3-22 04:42 作者: 漂白 時(shí)間: 2025-3-22 11:12
Extraction of DNA from Agarose Gels, elution of the DNA from the substrate. Other methods include hot phenol extraction of the DNA from the gel. The use of phenol is best avoided if possible because of safety and waste disposal concerns. Presented here are two alternative techniques that avoid these concerns, do not require the purcha作者: 有特色 時(shí)間: 2025-3-22 12:53
,Construction of Genomic Libraries in λ-Vectors,cloning sites facilitating the cloning of foreign DNA. In addition, λ-phage vectors have the following advantageous features: high cloning efficiency, a relatively large insert-size capacity, and suitability for screening using nucleic acid probes. In terms of gene screening, genomic DNA libraries a作者: 有特色 時(shí)間: 2025-3-22 17:58
Isolation of Plasmids from , by Boiling Lysis,described with different adaptations in a variety of protocol books (.,.). The quality of the isolated plasmid DNA is lower than that from an alkaline lysis miniprep, but it is sufficient for restriction analysis.作者: Judicious 時(shí)間: 2025-3-23 01:00
Cloning in Plasmid Vectors,d upon transformation of the recombinant molecule into a bacterial cell (.. and .) so that the DNA of interest can be investigated further. Cloning is an essential part of many experiments, including library generation (..) and expression studies (..).作者: 美學(xué) 時(shí)間: 2025-3-23 02:05
E. coli Plasmid Vectors978-1-59259-409-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 河潭 時(shí)間: 2025-3-23 05:33 作者: Semblance 時(shí)間: 2025-3-23 12:13
Dengue-Induced Ocular Complications,d upon transformation of the recombinant molecule into a bacterial cell (.. and .) so that the DNA of interest can be investigated further. Cloning is an essential part of many experiments, including library generation (..) and expression studies (..).作者: 畏縮 時(shí)間: 2025-3-23 16:02 作者: hedonic 時(shí)間: 2025-3-23 21:09
978-1-61737-391-6Springer Science+Business Media New York 2003作者: 易受騙 時(shí)間: 2025-3-23 22:23 作者: geriatrician 時(shí)間: 2025-3-24 05:11 作者: 定點(diǎn) 時(shí)間: 2025-3-24 09:43
Transport in Single Channel Quantum Wires,only used for the propagation and manipulation of recombinant DNA. . is an enteric rod-shaped Gram-negative bacterium with a circular genome of 4.6 Mb (.). It was originally chosen as a model system because of its ability to grow on chemically defined media and its rapid growth rate. In rich media, 作者: morale 時(shí)間: 2025-3-24 10:47
Exoticism and Consumption in Anne Enright’s a donor bacterium. The ability to transform or accept exogenous DNA is generally referred to as competence, although the term has been so widely used in different systems that it is difficult to generate an all-inclusive definition for competence. Natural competence occurs in a defined subset of bac作者: 解凍 時(shí)間: 2025-3-24 18:31
,Informationen und Tips für Gewürzverwender,nsformation (.,.). This procedure is an effective method for the transfer of DNA to a wide range of Gram-negative bacteria, such as ., and reports indicate that 10. electro-transformants per microgram of DNA can be achieved in this species (.,.). Electroporation is probably the most efficient and re作者: 粘連 時(shí)間: 2025-3-24 21:42
,Durch Spiroch?ten verursachte Krankheiten,DNA by conjugation is often termed . or ., as opposed to vertical transfer by which genetic information is transferred from mother to daughter cells. When genetic information is transferred by conjugation from the donor strain to the recipient strain, this population of recipient bacteria are called作者: 助記 時(shí)間: 2025-3-25 02:20 作者: STYX 時(shí)間: 2025-3-25 04:07
3.524arate the two. Bacteria are lysed with a solution containing sodium dodecyl sulfate (SDS) and sodium hydroxide. During this step, chromosomal as well as plasmid DNA are denatured. Subsequent neutralization with potassium acetate allows only the covalently closed plasmid DNA to reanneal and to stay s作者: vibrant 時(shí)間: 2025-3-25 08:05
3.524described with different adaptations in a variety of protocol books (.,.). The quality of the isolated plasmid DNA is lower than that from an alkaline lysis miniprep, but it is sufficient for restriction analysis.作者: Arthr- 時(shí)間: 2025-3-25 13:42 作者: Ambulatory 時(shí)間: 2025-3-25 16:40
3.524n endonuclease digestion (..), DNA sequencing (..), in vitro mutagenesis (..–.), transformation (.. and .), transfection, or probe generation. The principal methodologies of extraction, involving either alkaline lysis or boiling are well established (.) and are described in detail in . and .. These 作者: Progesterone 時(shí)間: 2025-3-25 20:52 作者: Coronation 時(shí)間: 2025-3-26 00:23 作者: 止痛藥 時(shí)間: 2025-3-26 06:41
Optimization of the Pillaring of a Saponite,litate this process, the number of steps should be minimized and each step analyzed to ensure that it has been completed successfully. Often, this analysis involves restriction-enzyme digestion of DNA constructs followed by gel electrophoresis to examine the results. It is also important to be able 作者: WITH 時(shí)間: 2025-3-26 10:03 作者: 吞下 時(shí)間: 2025-3-26 14:45 作者: Range-Of-Motion 時(shí)間: 2025-3-26 17:33 作者: Nebulizer 時(shí)間: 2025-3-26 21:07
Cost Standards for Efficient Competition,h (.). In bacterial cells, λ-phage employs one of two pathways of replication: lytic or lysogenic. Commonly, λ-phage vectors replicate via the lytic pathway. During lytic growth, the viral DNA is replicated manifold, a large number of phage gene products are synthesized, and progeny phage particles 作者: Definitive 時(shí)間: 2025-3-27 03:29 作者: 減少 時(shí)間: 2025-3-27 05:24 作者: Forage飼料 時(shí)間: 2025-3-27 11:13
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/300217.jpg作者: fixed-joint 時(shí)間: 2025-3-27 14:24 作者: prostate-gland 時(shí)間: 2025-3-27 18:55 作者: 蚊子 時(shí)間: 2025-3-28 00:41 作者: bromide 時(shí)間: 2025-3-28 03:36 作者: notification 時(shí)間: 2025-3-28 09:38
3.524he terminal sequences as well as the size of the amplified fragment and, in this way, provides freedom from the problems associated with location and abundance of restriction enzyme recognition sites.作者: 權(quán)宜之計(jì) 時(shí)間: 2025-3-28 13:26
Lorrie Miller,Biljana C. Fredriksenvector will both grow on appropriate selective medium; therefore, screening is almost always necessary to select colonies containing the recombinant plasmid and, in some cases, with the correct orientation of insert.作者: 反對(duì) 時(shí)間: 2025-3-28 17:05
Chemical Transformation of ,,ence is restricted to a subset of bacteria, methods for the chemical induction of a competent state in otherwise nontransformable bacteria are an important tool in bacterial genetics. For these species, competence refers to the ability to take up and propagate plasmid DNA, usually with no sequence specificity for uptake.作者: 色情 時(shí)間: 2025-3-28 22:10
Cosmid Packaging and Infection of ,,, usually ColE1, which regulates cosmid replication and copy number within the . host (.,.), and restriction sites that facilitate cloning of the desired DNA fragments and insert verification by restriction digestion (..).作者: 消瘦 時(shí)間: 2025-3-29 01:27
Isolation of Plasmids from , by Alkaline Lysis,lasmid DNA is concentrated from the supernatant by ethanol precipitation. Using this procedure, 2–5 μg of DNA can be obtained from a 1.5-mL culture of . containing a pBR322-derived plasmid, and three- to five-fold higher yields can be expected from pUC-derived plasmids (.).作者: ODIUM 時(shí)間: 2025-3-29 03:39 作者: modest 時(shí)間: 2025-3-29 08:41 作者: 懶鬼才會(huì)衰弱 時(shí)間: 2025-3-29 15:06 作者: CUR 時(shí)間: 2025-3-29 17:55 作者: Anthology 時(shí)間: 2025-3-29 19:44
Isolation of Cosmid and BAC DNA from ,,nd sequencing. Numerous standard protocols have been developed to isolate supercoiled plasmids, but of these, the alkaline lysis protocol remains the most suitable approach to isolate large-insert clones such as cosmids and BACs.作者: 無彈性 時(shí)間: 2025-3-30 01:29
Preparation of Single-Stranded DNA from Phagemid Vectors,e phagemids as ssDNA into viral particles, which are subsequently released into the culture medium (.). ssDNA isolated from these virions is suitable for use as a template in a variety of procedures with no further manipulation, as denaturation is not required prior to its use as a template.作者: 忍受 時(shí)間: 2025-3-30 06:13 作者: 誘騙 時(shí)間: 2025-3-30 11:35 作者: declamation 時(shí)間: 2025-3-30 14:47
1064-3745 d cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.978-1-61737-391-6978-1-59259-409-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 射手座 時(shí)間: 2025-3-30 16:49
Transport in Single Channel Quantum Wires,ic manipulation has subsequently solidified the role of . as the host of choice for the propagation, manipulation, and characterization of recombinant DNA. In the past 60 yr . has been the subject of intensive research and more is now known about these bacilli than any other organisms on earth.作者: Infant 時(shí)間: 2025-3-30 20:48
3.524, Protocol 10). Plasmid minipreps are a ubiquitous part of molecular biology. They are simple and easy to perform but can be very tedious when large numbers of samples are involved. In recent years, endeavors such as the human genome project have led to the development of methods for the manageable high-throughput extraction of plasmids (.–.).作者: 蠟燭 時(shí)間: 2025-3-31 03:10 作者: 難理解 時(shí)間: 2025-3-31 07:32
High-Throughput Plasmid Extraction Using Microtiter Plates,, Protocol 10). Plasmid minipreps are a ubiquitous part of molecular biology. They are simple and easy to perform but can be very tedious when large numbers of samples are involved. In recent years, endeavors such as the human genome project have led to the development of methods for the manageable high-throughput extraction of plasmids (.–.).作者: intolerance 時(shí)間: 2025-3-31 09:19 作者: 不易燃 時(shí)間: 2025-3-31 17:14 作者: 分貝 時(shí)間: 2025-3-31 18:20
Cubic Silicon Carbide Nanowires,: insert size, copy number, incompatibility, selectable marker, cloning sites, and specialized vector functions. Several of these criteria are dependent on each other. This chapter discusses these criteria in the context of choosing a plasmid for use as a cloning vector and . displays the features of some commonly used cloning vectors.作者: insipid 時(shí)間: 2025-3-31 23:13 作者: 裁決 時(shí)間: 2025-4-1 03:05 作者: 細(xì)菌等 時(shí)間: 2025-4-1 07:02
Optimization of the Pillaring of a Saponite,to easily track the DNA manipulations. This means that each step must be documented, illustrated, and stored as the project proceeds. It is also desirable to maintain an overview of the cloning strategy and track changes as they are made. Graphical representations of DNA constructs make the process simple to follow and less prone to errors.作者: 暫時(shí)休息 時(shí)間: 2025-4-1 13:15 作者: Hallmark 時(shí)間: 2025-4-1 15:13
Z. Dlouhy,D. Baiborodin,J. Mrázekribe exclusively or predominantly extrachromosomal genetic elements that replicate autonomously. Plasmids are now known to be present in most species of Eubacteria that have been examined, as well as in Archaea and lower Eukarya (.).
